Minimum Dataset Deliverable 4.2 December 2012 1 Table of Contents Executive summary.....................................................................................................................3 Introduction .................................................................................................................................4 Variable-rate irrigation................................................................................................................4 Irrigation scheduling .................................................................................................. 5 Results on water consumption ................................................................................. 14 Conclusions on precision irrigation ......................................................................... 18 Variable-rate fertilization ..........................................................................................................18 Fertilizer application ................................................................................................ 18 Crop response to variable-rate fertilizer application ................................................ 19 Comparison of variable-rate fertilizer application to farmer practice ..................... 20 Correlation of canopy reflectance to soil properties and cotton yield ..................... 21 Requirements for adoption of the technology.......................................................... 21 Summary and conclusions ....................................................................................... 23 Site-specific pest management ..................................................................................................36 Herbicide application ............................................................................................... 36 Insecticide application ............................................................................................. 36 Pesticide Use Efficiency .......................................................................................... 36 Digital weed mapping .............................................................................................. 37 Requirements for site-specific pest management .................................................... 38 Conclusions – Management decisions ..................................................................... 40 Parameters related to the development of regional GIS database.............................................41 Methodology ............................................................................................................ 41 2 Results ...................................................................................................................... 42 References .................................................................................................................................44 Minimum Dataset Executive summary The ground sensor technologies used in this project allowed the remote estimation of spatially-variable fertilizer and irrigation water within the growing season. When inputs estimated this way were applied to management zones that were delineated by differences in soil organic matter content, there was a significant reduction of water, chemical and energy inputs and the crop responded by an average of 10% yield gains in 2011. Considering the excessive and unguided applications by the farmers in the area, a substantial reduction of fertilizer and water inputs can be achieved by the adoption of multispectral sensors, soil moisture sensors and evapo-transpiration devices for uniform application of entire fields before somebody considers spatially-variable inputs. Most farmers in the area already possess the equipment needed to uniformly apply fertilizer and irrigation water at recommended rates without additional costs. For more efficient management of inputs within single fields, delineation of management zones and a spatially-variable application system is needed. However, variable rates of fertigation in different management zones would require a differential drip irrigation system which is associated with additional costs and farmer involvement. A decision support tool developed by the HydroSense project was the production of high-resolution maps of soil organic matter distribution across the Pinios watershed using the relationship between bare soil satellite NIR reflectance and soil nitrogen content. These maps can be used to delineate simple management zones within fields and, thus, reduce farmer costs associated with setting-up differential fertigation systems. A sound alternative to variable-rate fertilizer application by management zone is the employment of real-time and variable-rate technology that is expected to become commercially available in the next few years. Whether the ground sensor systems are used for uniform or spatially-variable fertilizer application within fields, they are associated with purchasing costs, farmer training and involvement. A feasibility study is required to identify possibilities and reach further conclusions for the adoption of this technology by individual farmers or networks or for provision of services by agricultural unions and private companies. Greater incentives for the adoption of the irrigation and fertilizer technologies can be provided through subsidies for the protection of environmentally sensitive areas or through the adoption of water pricing policies by local government. 3 Introduction This deliverable concerns the assessment of site-specific management in the demonstration sites during the summer growing seasons of 2010 and 2011. The site-specific management practices were applied by management zone in the cases of irrigation and N fertilization and by real-time variable-rate in the case of weeds. The report evaluates the degree of success of these new approaches at the field scale in terms of crop response, environmental performance and potential of adoption by producers. This deliverable also examines the potential of modeling key soil and plant properties that can be used to predict productivity patterns or delineate management zones at the watershed scale. For this purpose, soil or crop properties should correlate to individual wavebands or vegetation indices of satellite imagery so that classification and mapping of the entire watershed can be achieved for the purpose of regional-scale planning. Variable-rate irrigation The developed variable-rate irrigation system was able to provide irrigation water to each management zone independently. Figure 1 is a schematic layout of the components which make up the irrigation system used. The main supply of irrigation water (after pumping from irrigation canals, or from irrigation network) connected with a filtration system and a fertilizer injection device. A number of different drip irrigation lines ran along the crop rows to deliver water to the different management zones. A faucet and a hydrometer connect the drip irrigation pipes with the main system, thereby allowing the selective irrigation of the desired management zone and the measurement of total irrigation water applied. 4 Figure 1. Schematic presentation of the differential drip irrigation system used in Gyrtoni. Each of the three management zones is provided with an independent drip irrigation network. The drip irrigation lines run between the cotton rows. Irrigation scheduling Each management zone of the pilot areas was regularly monitored for the entire irrigation period in terms of water needs with the different systems of sensors. Every type of sensor gave different information: Smart Crop infrared system: Timing of irrigation, on-line monitoring of irrigation efficiency Watermark soil moisture sensors: evaluation of the effectiveness of rain and irrigation water, timing of irrigation Evapotranspiration device: volume of irrigation water to apply Smart Crop data for each management zone were continuously downloaded to the website. Soil moisture and evapotranspiration data were recorded manually every 2 days. The experience gained in the 2010 season was used for more timely and efficient use of the systems in 2011. In Gyrtoni and Omorfochori pilots, the timing of irrigation was determined by the combined utilization of the Smart Crop system and Watermark sensors. When canopy temperature began to decrease approaching ambient and watermark sensor readings were over 80-100kPa, then irrigation started. In the Gyrtoni pilot, the timing of irrigation was based solely on watermark readings because the Smart Crop system was not operational. Pilot plots were irrigated the same way to the controls, that is, uniformly during the first 6 irrigation events. In this way we avoided any water stress in the first growth stages of cotton that could have a yield-limiting effect. Variable rate irrigations started in early July 2011 when the crop started to demand greatest amounts of water. It was day 72 after planting for Gyrtoni, 74 for Omorfochori and 75 for Gentiki. Volume of irrigation was based mainly on the ET gage data. According to the growth stage of the crop, water demand was calculated and appropriate amount of irrigation water was applied in each management zone. Differences in the volume of irrigation water in the management zone of the same field reflect different soil water content as indicated by the soil moisture sensors (Figure 2). Differences in soil properties between management zones were met with the variable application of irrigation water. In Gyrtoni, higher concentration of clay and soil organic matter in zone A resulted in higher soil water holding capacity and less extreme variations in soil water content compared to zone B (Figure 2). 5 Figure 2. Watermark soil moisture readings by management zone for the 2011 irrigation period at Gyrtoni. Red lines indicate irrigations events. Evapotranspiration measurements were performed with ET gage every 2 days after cotton planting. Cumulative evapotranspiration for each of the pilot plots is shown in Figures 3, 5 and 7. Total evapotransiration for the Gyrtoni pilot plot was 729 mm. Figure 3. Cummulative evapotranspiration estimated by ETgage for the 2011 irrigation period at Gyrtoni 6 Variable-rate application of irrigation water resulted in different water volumes in the management zones reative to the control. Total irrigation water was also different among management zones (Table 1). Table 1. Volume of irrigation water applied (mm) at Gyrtoni in 2011 irrigation period Zone A Zone B Control 27/4 20 20 20 15/5 30 30 30 30/5 35 35 35 14/6 45 45 45 23/6 38,5 38,5 38,5 4/7 38,5 38,5 38,5 Variable rate irrigation 11/7 55,6 57,6 66 19/7 57,8 31,4 66 23/7 28,9 16,7 38,5 27/7 30,6 22,4 38,5 2/8 - - 38,5 8/8 61,7 59,6 38,5 17/8 49,9 33,7 44 20/8 31,09 43,2 44 30/8 0,44 12,0 38,5 Total 523 483 619 In the Omorfochori pilot, management zones responded to irrigation events in different ways and in some cases the volume of irrigation water differed between management zones. For example, zone A received a low volume of irrigation at 90 days after planting resulting in the continued decrease of soil moisture (increased sensor values). The opposite trend occurred at the same date in Zone B and C indicating more effective irrigation (Fig. 4). Ineffective irrigation was also evident in Zone B 95 days after planting in contrast to Zone A and C. Total evapotranspiration of the Omorfochori field was measured to be 737mm and was almost the same to that of the Gyrtoni field (Fig. 5). 7 Figure 4. Watermark soil moisture readings by management zone for the 2011 irrigation period in Omorfochori. Red lines indicate irrigation events. Figure 5. Cumulative evapotranspiration measured by ETgage for the 2011 irrigation period in Omorfochori 8 Table 2. Volume of irrigation water applied (mm) in Omorfochori during the 2011 season Zone A Zone B Zone C Control 3/5 30 30 30 30 10/5 25 25 25 25 25/5 30 30 30 30 7/6 35 35 35 35 18/6 40 40 40 40 30/6 40 40 40 40 Variable rate irrigation 5/7 45 45 45 57 12/7 50 50 50 57 18/7 33 33,4 33,4 38 21/7 25,8 38,9 26,2 38 27/7 9,7 51,2 34 38 3/8 40,4 16 46,2 47 10/8 13,6 21,4 15,5 38 17 17 17 17 15/8 26,6 42,1 30,5 38 22/8 35,3 50,1 39,3 47 30/8 21,8 29,4 24,4 38 Total 518,2 594,5 561,5 653 10/8 rain 9 Figure 6. Watermark soil moisture readings by management zone for the 2011 irrigation period in Gendiki. Red lines indicate irrigation events. Figure 7. Cummulative evapotranspiration estimated by ETgage for the 2011 irrigation period in Gentiki 10 Overall results indicated that the soil water sensors were able to monitor the soil water status and that measurements recorded by the systems reflected general trends of soil water fluctuations during the growing season. In Gentiki, the management zone with the higher concentration of soil organic matter and greater water holding capacity received the smallest amount of irrigation water. In contrast, zone C received 21% more irrigation water than zone A because of its low organic matter and water holding capacity. This confirms the basic reasoning that soil organic matter and clay content affect the volume of irrigation water applied in a spatially variable field. Table 3. Volume of irrigation water applied (mm) in Gendiki during 2011 season Zone A Zone B Zone C Control 10/5 25 25 25 25 26/5 20 20 15 15 13/6 50 50 50 50 26/6 55 55 55 55 6/7 60 60 60 60 Variable rate irrigation 12/7 57 57 57 57 18/7 65,4 44,0 73,7 57 22/7 22,9 20,9 33,2 38 26/7 18,4 22,6 18,0 38 1/8 5,9 42,2 45,8 57 10/8 32,9 33,0 54,0 57 15/8 9,0 24,8 42,2 38,0 20/8 15,9 15,2 26,1 38,0 25/8 23,5 21,3 32,3 38,0 1/9 17,3 15,7 25,2 19,0 ΣΥΝΟΛΟ 478 506 612 642 11 The daily crop evapotranspiration (ET) for cotton was estimated in each pilot area. The cumulative ET between the 3 pilots differed by about 2% - 7%. Farmers irrigate their plots with 85% up to 93% of the estimated evapotranspiration. The pilots were irrigated with 69% to 88% of the estimated evapotransiration which equated to 4% - 25% less than the controls (Table 4). Table 4. Comparison of 2011 irrigation data between pilot areas Field Gyrtoni Omorfochori ET(mm) Management Irrigation Zones water(mm) Percentage of irrigation water to control (%) 729 Control 619.5 84.9 100 A 523.11 71.7 84.4 B 594.5 81.5 95.6 652 88.4 100 A 518.2 70.3 79.4 B 594.5 80.6 91.1 C 561.5 76.1 86.1 642 92.7 100 A 478.2 69.1 74.4 B 506.7 73.2 78.9 C 612.5 88.5 95.4 737 Control Gentiki Percentage of irrigation water to ET (%) 692 Control 12 Table 5. Pearson correlations for irrigation data of the Gyrtoni pilot area ET_cum Irrigation Watermark Canopy_temp Zone A Irrigation -,334 Watermark ,347 -,252 Canopy_te -,426 ,282 -,015 δΘ -,010 ,006 -,239 -,392 Zone B Irrigation -,349 Watermark ,060 -,220 Canopy te -,259 ,103 ,057 δΘ -,063 -,002 -,201 -,612 Significant correlations between irrigation water, evapotransiration and canopy temperature were detected in some management zones of Gyrtoni and Omorfochori pilots. More specifically, canopy temperature of cotton plants was strongly correlated to evapotranspiration in Omorfochori (Table 6). Effects of irrigation on canopy temperature and soil water were observed with an increase in soil water within the root zone as shown by irrigation-canopy temperature correlations. This does not apply for Gyrtoni (Table 16) but there are several local and seasonal environmental factors (sunshine, air temperature, wind and humidity) which affect canopy temperature independent of transpiration that cause a loss of soil water (Padhi et al., 2012). Table 6. Pearson correlations for irrigation data of the Omorfochori pilot area ET_cum Irrigation Watermark Zone A Irrigation Watermark Canopy_temp -,050 ,250 ,158 -,703 ,445 13 -,099 Canopy_temp δΘ ,492 -,276 ,207 -,887 Zone B Irrigation -,020 Watermark -,085 ,138 Canopy_temp -,773 ,455 -,109 ,592 -,296 ,107 δΘ -,887 Zone C Irrigation Watermark Canopy_temp δΘ -,044 ,344 -,143 -,679 ,386 ,025 ,384 -,223 -,431 -,855 Although the monitoring of canopy temperature was useful for the timing of irrigation at Gyrtoni and Omorfochori, irrigation scheduling could only be performed by the monitoring of soil moisture and evapotranspiration in Gentiki. There are several levels at which canopy temperature data can be instructive. In real time or short (daily or weekly) timescales, temperature can be monitored to indicate irrigation system performance, rainfall utilization and the current stress status of the plant. The installation of a number of IR sensors strategically placed within a field or within management zones will allow the producer to see water deficits spatially within an irrigation system. When in-season rainfall provides a significant portion of the crop’s water requirement, monitoring of canopy temperature can result in significant reduction of irrigation water because it provides plant-based information on the water status of the crop. Since canopy temperature is collected on short time intervals (15 min in this study), it can provide a level of resolution that is unequaled by virtually all other measures of the plant. However, there are several issues affecting the performance of the Smart Crop system during field operation that seem to be important obstacles for its adoption by farmers without a relevant familiarity with the technology. Nonetheless, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Results on water consumption The results regarding water use in the 3 experimental fields include the water consumption for the entire irrigation period, the seed yield and the water use efficiency. 14 Water consumption refers to the sum of irrigation water applied and rainfall received during the growing season expressed in mm water. The yield expressed in kg of seed cotton /ha and irrigation water use efficiency was calculated from the water consumption and yield and expressed as kg of seed cotton /m3 irrigation water. Results from the Gentiki demonstration site are presented in Figure 8. With precision irrigation zone A received 34,23% (478,2mm H2O), zone B 26,7% (506,7mm H2O) and zone C 4,8% (612,5mm H2O) less irrigation water than was applied in the control (642 mm H2O). Cotton yield in zone A increased by 17,5%, in zone B increased by 11,2% and in zone C decreased by 1,4% in comparison to the corresponding zones of the control. Therefore, water use efficiency increased significantly in two management zones. Irrigation water use efficiency in Zone A (0.50 kg /m3 ) was 34,38% higher than the control (0.37kg /m3), in zone B (0.59kg /m3) 20% higher than the control (0.49kg /m3) and in zone C (0.45kg /m3) 11,9% less than the control (0.51kg /m3). WATER CONSUMPTION (mm H2O) COTTON YIELD (kg/ha) IRRIGATION WATER USE EFFICIENCY (kg/m3) 15 Figure 8. Results of water consumption, cotton yields and water use efficiency in the Gentiki demonstration site Results from Omorfochori demonstration site are presented in figure 9. With precision irrigation zone A received 26% (518,2mm H2O), zone B 9.8% (594,5mm H2O) and zone C 16,3% (561,5mm H2O) less irrigation water than that applied in the control. Cotton yield in zone A increased by 8,7%, in zone B by 14,5% and in zone C by 31% in comparison to the corresponding zones of the control. Irrigation water use efficiency in Zone A (0.66 kg /m3) was 27,3% higher than the control (0.48kg /m3), in zone B (0.61kg /m3) 19.7% higher than the control (0.49kg /m3) and in zone C (0.71kg /m3) 35,2%less than the control (0.46kg /m3). WATER CONSUMPTION (mm H2O) COTTON YIELD (kg/ha) IRRIGATION WATER USE EFFICIENCY (kg/m3) Figure 9. Results of water consumption, yields and irrigation water use efficiency in Omorfochori experimental field 16 Results from Gyrtoni demonstration site are presented in Figure 10. With precision irrigation zone A received 18.4% (523,11mm H2O) and zone B 28,1% (594,5mm H2O) less irrigation water than the control. Cotton yield in zone A decreased by 10,9% (4154 kg/ha) and in zone B by 18% in comparison to the control (4664 kg/ha). Water use efficiency obtained in Zone A (0.7 kg /m3) was 4,9% higher than the control (0.67kg /m3) and in zone B (0.79kg /m3) 5% higher than the control (0.49kg /m3). WATER CONSUMPTION (mm H2O) COTTON YIELD (kg/ha) IRRIGATION WATER USE EFFICIENCY (kg/m3) Figure 10. Results of water consumption, cotton yield and water use efficiency in Gyrtoni experimental site. 17 Conclusions on precision irrigation The results indicated the great potential of precision irrigation to reduce irrigation water without adversely affecting cotton yields in the region of the Pinios River Basin. Since yield data are directly affected by climatic conditions, more years of experimentation are required to precisely quantify the extent of water savings with the adoption of the precision irrigation techniques used. Current attitudes of farmers towards irrigation, fertilization and crop protection practices are based mostly on their empirical assumptions concerning the needs of the crop, with minimal consideration given to the interlinked environmental impact, energy consumption and potential cost savings. Precision agriculture can contribute to more efficient irrigation of cotton in the region with environmental and economical benefits. The adoption of these technologies by farmers remains a critical issue because of the need to link new management practices with clear economic benefits. Farmers may not be easily convinced of the capacity of the novel technologies introduced to them, which seems to challenge their acquired empirical knowledge and experience gained over the years on fundamental crop management as many of them are totally unaware of new irrigation technologies. The application of precision irrigation by the HydroSense project indicated that soil moisture sensors and evapo-transpiration devices can be readily adopted by farmers regardless of the creation of different management zones within the fields. The reasons are their low purchasing costs, simplicity of use and data interpretation. In this project, evapotranspiration was used to estimate volume of irrigation water and soil moisture sensors were used to determine timing of irrigation. The production of soil organic matter, soil texture or cotton yield maps will allow the strategic or guided placement of soil moisture sensors within a field. It is estimated that the adoption of the above technologies could cost under 40 euros/ha. Training of farmers on the use of these instruments could be achieved by the creation of a network between farmers and researchers in the region and expanded to include other innovative technologies in the future for more efficient water use in the Pinios River Basin. The ability of the infrared sensors to directly detect crop water stress could provide growers or consultants more effective methods to manage water stress by management zone within fields. However, the cost of these tools coupled with needed expertise for their use make their adoption by the farmers a difficult task at present. Variable-rate fertilization Fertilizer application The applied method for spatially-variable fertilizer application used a N application model that directly relates normalized sensor measurements to a generalized plant growth function (Holland and Schepers 2010). The sensor measurements were in the form of a Chlorophyll 18 Index (CI=( NIR / Red edge)-1) that was computed from individual waveband reflectance values of the Crop Circle sensors. This vegetation index has been shown to accurately estimate (R2=0.95) the canopy chlorophyll content (g m-2) under contrasting conditions of canopy architecture and leaf structure represented by species of soybean and maize. Thus, CI can be applied to estimate canopy chlorophyll in other crops or under mixed pixel scenarios (Gitelson et al. 2005). The sensor normalization technique used by the N application model is referred as the sufficiency index (SI) which is the ratio of the sensed canopy CI to a reference CI. A unique reference CI was used for each growth stage of the crop in each pilot area by the identification of non-N limiting plants from the extracted CI maps (Holland and Schepers 2011). The N application model has the following mathematical form: where Nopt is the maximum N rate or the crop N assimilating capacity, Nprefert is the sum of fertilizer N applied prior to crop sensing, Nom is the N credit for the field’s average organic matter content, SI is the sufficiency index, ΔSI is the sufficiency index difference parameter, m is the back-off rate variable (0<m<100) and SIt is the back-off cut-on point. Segmented fertilizer application within the growing season occurred in 2 to 4 increments of 20 to 40 kg N/ha in the pilot areas via the drip irrigation system (Table 7). The amount of fertilizer to be applied in each management zone was calculated by the N application model that converts canopy CI to N requirement. In practice, the management zones of some pilot areas received the same amount of N in the initial fertilizer applications due to delays in the installation of the irrigation systems (2010) or due to farmer interference (2011). In these cases, only the final application determined the difference in the amount of fertilizer between management zones in the pilot areas (Table 7). Crop response to variable-rate fertilizer application The response of canopy chlorophyll index (CI) was positive to within-season fertilizer applications and indicated that CI was a sensitive indicator of crop N uptake (Fig. 11 and Fig. 12). The magnitude of the response varied with pilot area, management zone and growth stage of the crop. These interacting factors explain the lack of overall correlation between canopy CI and rate of N application. For example, the plants of the Gentiki pilot area had a greater response to fertilizer inputs in both growing seasons in comparison to other pilot areas. The maximum response occurred with a 20 kg N/ha fertilizer application at 63 DAP of 2011 (Fig. 12) with a 0.65 unit increase of CI corresponding to 150% increase relative to the CI of the canopy prior to fertilizer application. A smaller increase in CI of 0.20 units in the previous fertilizer application at 49 DAP (Fig. 12) was attributed to the lower N demand of younger plants. The effect of late growth stage appears to be the reason for the little CI response to N inputs at 122 DAP in the Gyrtoni pilot area in 2010 (Fig. 11). The plants in the Omorfochori pilot area in 2011 responded equally well to N applications at 64 and 73 DAP (Fig. 12) with an average CI increase by 0.2 units or by 20 to 60% increase relative to the CI of the canopy prior to fertilizer application. 19 A histogram comparison of CI between pilot areas and sampling times is shown in Figures 13 and 14. Histograms reveal features of the distribution such as skewness, range and double humps of plant vigor within fields that are not evident in conventional tables of descriptive statistics. During early growth (up to 73 DAP) there was great variability of plant sizes as indicated by the wide range of CI and exposure of sensors to bare soil as indicated by the low CI values in 2010 (Fig. 13). The uniformity of plant growth within fields increased progressively from 90 DAP to 110 DAP as indicated by narrower CI ranges. The highest CI values indicated greater plant vigor in the Gentiki pilot area at 110 DAP and lowest CI values were obtained in the Eleftherio pilot area in 2010 (Fig. 13). Similar temporal patterns were evident in the 2011 pilot areas although comparative field measurements were available up to 89 DAP (Fig. 14). During early growth, the histograms of Omorfohori and Gyrtoni had the appearance of a double hump which is typical when two management zones are expressed in terms of plant vigor. The disappearance of the double humps and the narrow range of CI later in the season (Fig. 14) indicated that our differential fertilizer applications accomplished uniformity of plant vigor within fields. Compared to the other two pilot areas, Gyrtoni had higher CI values and, thus, greater chlorophyll content from early growth till 89 DAP. The two Gyrtoni fields in 2010 and 2011 at 90 DAP had similar CI histograms both in terms of range and magnitude. Differences in CI between management zones were persistent within the growing season in Gentiki, Gyrtoni and Omorfochori (Fig. 12) despite the application of variable-rate fertilizer inputs to optimize growth (Table 7). The stability of the differences in CI indicated limitations in N uptake imposed by differences in soil fertility between management zones. CI differences between management zones were small in the Eleftherio and Gyrtoni pilots in 2010 (Fig. 11). Within the more effective 2011 season, average maximum CI differences between management zones were between 10 and 30% that translated into 9 to 13% differences in applied N fertilizer. Comparison of variable-rate fertilizer application to farmer practice A comparison of the timing of fertilizer N applications in the pilot and control areas relative to crop N demand is shown graphically in Fig. 15 and 16. In-season fertilizer applications occurred in advance of crop N needs in Gentiki and Omorfohori in 2011 (Fig. 16). The poor synchronization of soil available N with crop demand indicated increased risk of N losses to the environment. Due to lack of significant summer rainfall, these losses would be caused by greater leaching and denitrification in the control areas due to excessive irrigation of the control areas by the farmer. The total amount of fertilizer applied by the farmers exceeded crop N assimilation capacity in both fields (Fig. 16) without considering the additional sitespecific N sources available to the crop. These would be of the order of 100 kg N/ha (Table 8) which means that a total in excess of 110 kg N/ha would be lost to the environment in the control areas. In contrast, the N requirement estimated by the sensor-N model system resulted in final fertilizer N rates below the crop N requirement in the pilot areas (Fig. 16). By adding the available site-specific N sources to the applied N (Table 8), an excess of 50-70 kg N/ha would occur in the pilot areas at the end of the growing season. But this is a safe margin considering that some fertilizer N losses would incur during the growing season. The greater yield of the pilot areas justifies our approach. The fertilization procedure in the third 20 pilot area (Gyrtoni 2011) was not as successful despite the appropriate timing of N inputs. The total amount of available N (fertilizer + site-specific N = 230 kg N/ha) was well below the exceptionally high crop N demand (300 kg N/ha) in management zones A and B. This underestimation of crop assimilation capacity, as an integral component of the N application model, resulted in under-fertilization of the pilot management zones. The greater amount of fertilizer N applied by the farmer resulted in greater cotton production in the control areas. The 2010 growing season was characterized by large amounts of early-season soil residual N in the Gentiki and Gyrtoni sites (Table 8) that was the reason to avoid preplant fertilizer application and to reduce in-season fertilizer N in the pilot areas by the N application model (Fig. 15). However, the slightly lower yield attained in the Gentiki and Eleftherio pilot areas, compared to their respective controls, was perhaps caused by delayed fertilizer applications at a time when most soil N was taken up by the crop (Fig. 6). The estimates of crop N uptake were probably underestimated in the Gyrtoni site in 2010 (Fig. 13) due to a severe pest damage that resulted in reduced cotton yield. For this reason, our N strategies are not evaluated for this site. Differences in the amount of fertilizer applied were much greater between the pilot and the control areas than between management zones within the pilot areas. On average, 57% less fertilizer was applied in the pilot areas with only an 8% yield loss in 2010 and 34% less fertilizer with an actual yield gain in 2011 (Tables 9 and 10). These values translate to a great increase of NUE in the pilot areas, 75% greater NUE in 2010 (Gyrtoni site omitted) and 65% greater NUE in 2011, which mean respective savings in N losses compared to the farmer practice under long-term steady-state conditions. The large differences in fertilizer rates and environmental performance indicators between the control and pilot areas indicate that the large fertilizer doses by the farmers were excessive despite their attempt to synchronize N inputs with crop needs by multiple within-season applications. Correlation of canopy reflectance to soil properties and cotton yield Canopy CI of management zones, before the application of in-season N fertilizer and differential irrigation at 70 DAP, was correlated to cotton yield, applied fertilizer, irrigation water and NUE in different years. But one consistent positive correlation across pilot zones and years was between CI and WUE indicating that management zones of high canopy CI values had average to high cotton yields and low needs for irrigation water. This relationship would suggest that vegetation indices such as CI or NDVI of ground sensors or satellite imagery could serve as input variables in the GIS model to predict areas of high productivity and low irrigation needs and provide useful scenarios for water pricing and recovery of irrigation costs at watershed scale. Requirements for adoption of the technology However, the main purpose of monitoring canopy CI was to apply variable-rate N within fields and the sensor technology is designed for this purpose. The large and excessive fertilizer doses applied by the farmers indicate that the use of our N application model would reduce fertilizer inputs, optimize yields and protect the environment. As a first step, the use of our sensor data in an N application model for the estimation of a uniform application in the entire field would bring a spectacular improvement in farmer N 21 management practices before somebody considers monitoring N inputs by management zone. This can be achieved by a single pass of the sensor-carrying vehicle within the growing season at 70 to 80 DAP after an initial pre-plant application of about 20 to 40 kg N/ha. The exact amount of preplant N application can be determined by the residual nitrate content in the soil. The results of the 2011 growing season indicate that a reduction of fertilizer N by 30% can be achieved in this way. This figure was calculated by comparing the maximum fertilizer rate per management zone of each pilot area to that applied by the farmer. The sensor-N application model has advantages over the traditional N mass balance approach in that it accounts and corrects for variable weather conditions and soil organic matter mineralization during the growing season. An alternative to the sensor equipment is the use of satellite data in the N application model. Satellite imagery has the advantage of delivering spectral information rapidly over relatively large areas of the landscape. The new satellite imagery of World View 2 has the appropriate resolution and wavebands (including red edge) to compute the spatial variability of the chlorophyll index within single fields. However, the information provided by airborne platforms may not be available in time to implement critical management decisions. This is because the availability of airborne sensor data is constrained by weather conditions, revisit frequency and elaborate data processing. The ground sensing system used in this project has no such limitations. For spatially-variable fertilizer applications, experienced farmers would be able to visually identify distinct management zones within their fields without the need to employ remote sensing technologies and ground-truthing procedures. However, different fertilizer rates in different management zones would require a differential irrigation system considering that it is a common practice in the area to apply liquid fertilizer through drip irrigation. A differential irrigation system is associated with additional costs and involvement which the farmers may not have. A sound alternative to variable-rate application by management zone is the employment of real-time and variable-rate technology that is expected to become commercially available in the next few years. Employment of real-time technology does not require the delineation of fields into management zones and the set-up of differential irrigation systems while it is designed to maximize yields and NUE by spatially optimizing N inputs. Whether the sensor system is used for uniform or spatially-variable N application within fields, it is associated with purchasing costs, farmer training and involvement. The current price of N fertilizers will not justify the use of such equipment unless producers manage larger fields of the order of 100 ha where fertilizer savings can be significant. Most farmers in the area will manage multiple fields of a total area of 50 ha or less. A feasibility study is required to identify possibilities and reach further conclusions for the adoption of this technology by individual farmers or for provision of services by agricultural unions and private companies. Greater incentives for the adoption of the technology can be provided through subsidies for the protection of environmentally sensitive areas. This is a realistic scenario because such subsidies have been given in the past for the protection of groundwater quality by limiting the use of N fertilizers by farmers in the Thessaly Plain. 22 Summary and conclusions The sensor technology used in combination with the N application model allowed the remote estimation of spatially-variable crop N requirement within the growing season. When fertilizer N estimated this way was applied to management zones that were delineated by differences in soil organic matter content, the crop responded to each segmented application and an average reduction of N inputs by 35% with 10% yield gains was achieved in 2011. The remote estimation of crop N requirement is a superior tool for spatially-variable and site-specific N management when compared to conventional methods (mass balance approaches, intensive soil and plant sampling). The lack of significant correlations between canopy chlorophyll index and measurable soil properties indicated the magnitude of interacting factors in determining crop N uptake and yield potential and the need of using this technology on a field-by-field basis. A consistent relationship between CI and WUE indicated that CI could possibly serve as an input variable in the GIS model to predict areas of low irrigation needs and, thereby, provide useful scenarios for water pricing and recovery of irrigation costs. Considering the excessive and unguided fertilizer applications by the farmers in the area, a significant reduction of fertilizer N inputs can be achieved by the adoption of this technology for uniform application of entire fields before somebody considers spatially-variable N inputs. Remotely-sensed data can be obtained by ground sensors used in this project or by appropriate satellite imagery and used to estimate N requirement by the N application model. However, additional information will be needed in order to adjust the N application model to individual fields (site-specific N sources, expected yield, planting date and timing of application). Most farmers in the area can provide the required data and possess the equipment needed to uniformly apply fertilizer at recommended rates with no additional costs. For more efficient N management within single fields, a spatially-variable N application system is needed. However, different fertilizer rates in different management zones would require a differential irrigation system which is associated with additional costs and involvement which the farmers may not have. A sound alternative to variable-rate application by management zone is the employment of real-time and variable-rate technology that is expected to become commercially available in the next few years. Whether the sensor system is used for uniform or spatially-variable N application within fields, it is associated with purchasing costs, farmer training and involvement. A feasibility study is required to identify possibilities and reach further conclusions for the adoption of this technology by individual farmers or for provision of services by agricultural unions and private companies. Greater incentives for the adoption of the technology can be provided through subsidies for the protection of environmentally sensitive areas. 23 Table 7. Segmented fertilizer applications by pilot area and management zone. Pilot area Gentiki Fertilizer applied (kg N/ha) Zone A Zone B Zone C 40 40 40 23 23 23 20 20 20 43 32 38 126 115 121 -9 -4 Year 2011 Application 1 2 3 4 Sum % zone diff. DAP -21 49 63 79 Omorfohori 2011 1 2 3 4 5 Sum % zone diff. -14 64 73 73 90 40 29 17 17 32 135 40 29 17 17 15 118 -13 Gyrtoni2 2011 1 2 3 4 Sum % zone diff. -15 86 95 111 50 25 30 13 118 50 15 25 13 103 -13 Gentiki 2010 1 2 3 Sum % zone diff. 75 96 103 30 23 22 75 30 23 22 75 0 Eleftherio 2010 1 2 Sum % zone diff. 50 99 50 22 72 35 10 45 -38 Gyrtoni1 2010 1 Sum % zone diff. 122 35 35 25 25 -29 24 40 29 17 17 25 128 -5 30 23 0 53 -29 35 35 0 25 Table 8. Estimation of site-specific N sources available to the crop, other than fertilizer, in each demonstration site. Gentiki 2010 Eleftherio Gyrtoni1 Gentiki 2011 Omorfohori Gyrtoni2 Early-season soil nitrate N Early-season available N† Soil organic N Legume N‡ Irrigation N Manure N‡ 170 111 40 0 20 0 77 50 40 0 20 0 223 145 40 0 20 0 40 26 40 0 20 0 62 40 40 0 20 0 93 60 40 0 20 0 Total available N sources 171 110 205 86 100 120 † assuming 35% N losses during the growing season ‡ sites had no recent history of legume rotations or manure applications 26 Table 9. Comparison of fertilizer application and nitrogen-use efficiency (NUE) between pilot and control management zones in 2010 Field Zone -1 Fertilizer N (kg ha ) Pilot Control % Diff. -1 Seed N (kg ha ) Pilot Control % Diff. Pilot NUE Control % Diff. Eleftherio Eleftherio A B 72 45 130 130 -45 -65 49 44 54,1 51,8 -10 -16 0,68 0,98 0,42 0,40 62 145 Gyrtoni1 Gyrtoni1 Gyrtoni1 A B C 35 25 35 129 129 129 -73 -81 -73 42 42 39 44,6 44,0 41,2 -5 -6 -5 1,21 1,66 1,11 0,35 0,34 0,32 246 388 247 Gentiki Gentiki Gentiki A B C 75 75 53 110 110 110 -32 -32 -52 62 58 52 66,8 65,0 54,2 -6 -11 -5 0,84 0,77 0,98 0,61 0,59 0,49 38 31 100 Table 10. Comparison of fertilizer application and nitrogen-use efficiency (NUE) between pilot and control management zones in 2011 Field Zone Fertilizer N (kg ha-1) Pilot Control % Diff. Seed N (kg ha-1) Pilot Control % Diff. Pilot NUE Control % Diff. Gyrtoni2 Gyrtoni2 A B 118 103 158 158 -25 -35 74 76 82a 93a -11 -18 0.62 0.74 0.52 0.59 20 26 Omorfo Omorfo Omorfo A B C 118 101 111 181 181 181 -35 -44 -39 68 73 79 63 64 60 9 15 31 0.58 0.73 0.72 0.35 0.35 0.33 68 106 115 Gentiki Gentiki Gentiki A B C 126 115 121 179 179 179 -29 -35 -32 47 59 54 40 53 55 17 11 0 0.37 0.51 0.45 0.23 0.30 0.31 66 72 45 a - small sample size 27 28 Gentiki 2010 1.3 1.2 Sensor CI 1.1 1.0 B 0.9 A C 0.8 0.7 0.6 60 80 100 120 140 Days after planting Gyrtoni 2010 1.4 1.2 AB C Sensor CI 1.0 0.8 0.6 0.4 0.2 0.0 60 70 80 90 100 110 120 130 140 150 Days after planting Eleftherio 2010 1.4 1.2 Sensor CI 1.0 0.8 0.6 A 0.4 B 0.2 0.0 60 80 100 120 140 Days after planting Fig. 11 Canopy Chlorophyll Index (CI) during the 2010 growing season by management zone (A, B, C) in each pilot area. Arrows indicate the timing of fertilizer applications. 29 Gentiki 2011 1.4 1.2 Sensor CI 1.0 0.8 B 0.6 A C 0.4 0.2 0.0 40 50 60 70 80 90 100 110 100 110 Days after planting Omorfohori 2011 1.4 1.2 1.0 Sensor CI BC 0.8 A 0.6 0.4 0.2 0.0 40 50 60 70 80 90 Days after planting Gyrtoni 2011 1.4 1.2 Sensor CI 1.0 B A 0.8 0.6 0.4 0.2 0.0 40 50 60 70 80 90 100 110 Days after planting Fig. 12 Canopy Chlorophyll Index (CI) during the 2011 growing season by management zone (A, B, C) in each pilot area. Arrows indicate the timing of fertilizer applications. 30 31 Eleftherio 2010 800 800 600 600 Count Count 71-73 DAP Gentiki 2010 400 200 400 200 0 0 0.0 0.2 0.4 0.6 0.8 1.0 1.2 0.0 1.4 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Gyrtoni1 2010 Chlorophyll Index Chlorophyll Index 800 1200 1000 800 600 800 600 400 Count Count Count 86-90 DAP 1000 600 400 400 200 200 0 0.0 0.2 0.4 0.6 0.8 1.0 1.2 200 0 0 1.4 0.2 0.4 Chlorophyll Index 0.8 1.0 1.2 0.2 1.4 0.4 0.6 0.8 1.0 1.2 1.4 Chlorophyll Index Chlorophyll Index 1000 1800 800 1600 800 1400 600 1200 600 400 1000 Count Count Count 110-115 DAP 0.6 800 400 600 200 200 400 200 0 0.0 0.2 0.4 0.6 0.8 Chlorophyll Index 1.0 1.2 1.4 0 0 0.2 0.4 0.6 0.8 1.0 1.2 Chlorophyll Index 1.4 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Chlorophyll Index Figure 13. Histogram comparisons of the distribution of Chlorophyll Index [(NIR/Red edge)-1] by pilot area and days after planting in 2010. 32 Gentiki 2011 Gyrtoni 2011 Omorfohori 2011 180 2500 1000 2000 800 1500 600 140 120 100 80 Count Count Count 57-61 DAP 160 1000 400 500 200 60 40 20 0 0 0 0.2 0.4 0.6 0.8 1.0 1.2 0.0 1.4 0.2 0.4 0.6 0.8 1.0 1.2 0.0 1.4 300 250 0.2 0.4 0.6 0.8 0.6 0.8 1.0 1.2 1.4 1000 800 200 600 Count Count 150 Count 72-76 DAP 250 200 150 400 100 100 200 50 50 0 0 0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 0.2 0.4 0.6 0.8 1.0 1.2 0.0 1.4 0.2 0.4 1.0 1.2 1.4 Chlorophyll Index 600 700 600 500 Count 400 Count 83-89 DAP 500 300 400 300 200 200 100 100 0 0 0.2 0.0 0.2 0.4 0.6 0.8 1.0 1.2 0.4 0.6 0.8 1.0 1.2 1.4 1.4 Chlorophyll Index Chlorophyll Index Figure 14. Histogram comparisons of the distribution of Chlorophyll Index [(NIR/Red edge)-1] by pilot area and days after planting in 2011. 33 Benaki Phytopathological Institute Gentiki 2010 200 Cumulative kg N ha -1 150 Control 100 Zone A, B 50 Zone C 0 0 20 40 60 80 100 120 140 Days after planting Gyrtoni 2010 200 Cumulative kg N ha -1 150 Control 100 50 Zone A, C Zone B 0 0 20 40 60 80 100 120 140 Days after planting Eleftherio 2010 200 Cumulative kg N ha -1 150 Control 100 Zone A 50 Zone B 0 -40 -20 0 20 40 60 80 100 120 140 Days after planting Fig. 15. Timing and rates of fertilizer N applied in the pilot zones and the controls relative to crop N uptake (shaded in gray) in 2010. The crop N uptake curve was estimated from crop yield at the end of the growing season. 34 Benaki Phytopathological Institute Gentiki 2011 Cumulative kg N ha -1 300 200 Control 100 Zone B Zone A 0 -40 -20 0 20 40 60 80 100 120 140 Days after planting Omorfohori 2011 Cumulative kg N ha -1 300 200 Control Zone A 100 Zone B 0 -40 -20 0 20 40 60 80 100 120 140 Days after planting Gyrtoni 2011 Cumulative kg N ha -1 300 200 Control Zone A 100 Zone B 0 -40 -20 0 20 40 60 80 100 120 140 Days after planting Fig. 16. Timing and rates of fertilizer N applied in the pilot zones and the controls relative to crop N uptake (shaded in gray) in 2011. The crop N uptake curve was estimated from crop yield at the end of the growing season. 35 Benaki Phytopathological Institute Site-specific pest management Herbicide application During the growing periods of 2010 and 2011, for band application of herbicides, individual nozzles were selected to be on only when they were spraying the cotton line and all the other were shut. By doing so, there was a band (approximately 35cm) of applied herbicide that followed the planting line. Comparing to control fields, band pre-emergence applications at pilot fields led to an average 57% herbicide saving for 2010 and 60% for 2011. During 2010 experimental year, in Gyrtoni and Gentiki sites there were few weeds left after the band application and the first mechanical cultivation which is the common practice for cotton in the region. Therefore, site-specific glyphosate application with WeedSeeker was not proven necessary. In contrast, Eleftherio site had a high number of purple nutsedge and the application of the WeedSeeker resulted in 55% glyphosate savings. Similar to 2010, in 2011 two of the three pilot fields lacked severe weed infestations making WeedSeeker application unjustified. However, in the third field (Omorfohori) where WeedSeeker did applied, herbicide savings were raised to 75% comparing to conventional management. Insecticide application Insect monitoring was done for major cotton pests in the area,in the three pilot cotton fields of the project. Major pests are considered the pink bollworm, Pectinophora gossypiella, and the American bollworm Helicoverpa armigera. The first is a local pest with relatively stable populations every year, whereas the second has occasional outbreaks. Monitoring was performed for a total of five insect pests, namely: Pectinophora gossypiella, Helicoverpa armigera, Agrotis segetu, Agrotis exclamationis, Agrotis litigiosus. The levels of incidence for the three noctuide pests (Agrotis sp.) are unknown in the area. Monitoring was based on trap captures of pheromone traps, a preferable method for these pests. Delta traps were used for P. gossypiella and funnel traps for the rest of the species. One trap per species was used in each field so three traps per species in total for all pilot areas. In the three pilot sites, for both 2010 and 2011 growing seasons, all pesticide applications for the most important pest (P. gossypiella) were done after monitoring the adult insects in the traps and estimation of the ball damage in the crop. In all locations there were no catches of S. littoralis, so there is no evidence for the presence of this pest in the widely area. Pesticide Use Efficiency Herbicide use efficiency was compared between the pilot and the control areas because there was no differentiation on herbicide sprayings between management zones. Data showed that the weed management strategies (band applications, use of the WeedSeeker spraying system) resulted in significant higher use efficiencies in the pilot areas. 36 Benaki Phytopathological Institute Band applications of herbicides result in significant herbicide savings and provide chemical weed control only in the area where competition of weeds to the crop is necessary to be retained. According to the literature, herbicide applications using special systems with sensors (such as the WeedSeeker system) in the directed-post emergent applications with hoods, herbicide savings were ranged from 40-70%. During the 2010 growing season, total amount of herbicides used was 57, 59, and 54% lower in the pilot areas compared to the control areas for Eleftherio, Gyrtoni1 and Gentiki, respectively. As a result, the total HUE was 104, 131, and 101% lower in the control areas compared to the pilot areas for Eleftherio, Gyrtoni1 and Gentiki, respectively. For Elefterio, Gyrtoni1 and Gentiki the Band-HUE was 113, 131, and 101% lower in the control areas compared to the pilot areas, respectively. For Elefterio, the WeedSeeker-HUE was 94% lower in the control areas compared to the pilot area. During the 2011 growing season, total amount of herbicides used was 60, 68, and 60% lower in the pilot areas compared to the control areas for Gyrtoni2, Omorfohori and Gentiki, respectively. As a result, the HUE was 114, 268, and 171 lower in the control areas compared to the pilot areas for Gyrtoni2, Omorfohori and Gentiki, respectively. For Gyrtoni2, Omorfohori and Gentiki, the B-HUE was 114, 195, and 171% lower in the control areas compared to the pilot areas, respectively. For Omorfohori the WeedSeeker-HUE was 372 lower in the control areas compared to the pilot areas, respectively. Digital weed mapping The objective was to spatially record and map weed density in cotton fields with the combination of four different methodological approaches as follows: traditional field work, the use of RGB field photographs, of multispectral ground-based sensors and on the analysis of high resolution satellite images. Traditional field work. The actual state of weed distribution identifying and counting the existing weed species in situ was done in preselected ground points inside the borders of the pilot farm. The selection of these points was primarily based on a macroscopic observation of weed occurrence in the study field and secondly on a spatially interpolated map of NDVI values, which was derived from the groundbased sensors’ readings produced earlier in June 2011. The number of the predefined points were selected in order to fully cover the classes of NDVI values produced by interpolating proximal sensors’ readings. In each point, monitoring was done on 9 frames (0.5 x 0.5 m each) on the ground between four adjacent rows of cotton. This is a very laborious work but a necessary procedure for validation of the other 3 methods RGB field photographs. All aforementioned frames and weeds contained in them, for each control point were photographed with a digital camera, which was mount on a tripod at 1.0 m distance from ground level. Image processing was carried out on a PC with MATLAB software. A specific image analysis algorithm was developed for the automatic calculation of weed cover percentage contained in each photograph. In this methodology, field work was significantly reduced and by using the specific algorithm, sound estimations of weed density was achieved. However, this procedure is not a real-time one since it requires a lot of processing work. Multispectral sensor readings. Weed monitoring between cotton rows was done using multispectral sensors connected to dGPS so that the output readings were georeferenced 37 Benaki Phytopathological Institute reflectance measurements in red, near-infrared, red-edge bands and also spatial referenced values of the spectral vegetation indices red-edge NDVI and NDVI. During the growing period of 2012, a new set of field sites were deployed in order to validate this method as compared with the other previous two methods. The use of proximal groundbased multispectral sensors in weed monitoring, which is based in the relation between reflection measurements and spectral signatures of weed flora, is proved to retain the advantage of quick and reliable estimation of weed presence across a cotton field. This technology as long as it is used in combination with field work, which ultimately verifies its results, can offer a significant solution to digital weed management. The most important weakness of this procedure comes from the fact that there is no estimation of the weed presence just in the sowing line but only through the closest proximal points from points within crop lines. Remote sensing data. In HydroSense, we used resolution merge techniques (pansharpening) to produce high resolution, multispectral imagery. In this way, the interpretability of the data is improved by having high resolution information which is also in colour. This methodology has got the clear advantage of estimations of weed presence at a larger scale (watershed level). However, it shows potential use at field level particularly in crops such as cotton where the sowing lines are 98cm giving enough space for proper weed detection. Requirements for site-specific pest management Collecting data. Remote sensing is the term used to describe the acquisition of information from far away locations such as satellite-based and aircraft-based sensors. The advantage of using multispectral images collected in this way is that large areas can be rapidly and repeatedly covered throughout a growing season. In general, by examining the reflectance values at certain regions of the electromagnetic spectrum it is possible to differentiate various targets like crops, weeds and soil. For monitoring weed and pest outbreaks a very high resolution (≤ 50 cm) is rather needed. The cost of purchasing imagery over a desired area is usually dependent upon the spatial resolution and as far as weed identification and mapping is concerned, where precision is needed, the cost is high and thus prohibitive for an average producer in Greece. Pesticides and herbicides are the two of the most expensive controllable costs in cotton production. Remote sensing technology and image processing analyses offer a potential to diminish these expenses. Mapping weeds using multispectral imagery prior to crop emergence or between crop rows is relatively straightforward as in most cases weeds are not randomly distributed across a field but are grown in patches. These weed patches can be detected by the presence of biomass in contrast to bare soil spectral reflectance. The calculation of weed cover through image analysis techniques is rather easy and is dependent on the image resolution and hence on/off prescription maps for spraying weeds with a minimum amount of herbicide is a true perspective. On the contrary, species identification, or discrimination between weeds and crop plants is a difficult task. The experience from the project indicates that with the use of multispectral imaging this kind of procedure in not applicable and that hyperspectral imaging could be of great help towards this direction. Under the same notion, pest and disease infestations can be detected with remote sensing techniques as long as they alter leaf chlorophyll, leaf area index or biomass. On time recognition of infested areas can lead to early zone spraying, eliminating insects with 38 Benaki Phytopathological Institute minimal amount of pesticides. However, it should be noted that the mobility of insects makes their site-specific management much more difficult than that of weeds. In both weed and insect monitoring of great importance is the temporal factor, meaning that data collection should be implemented at the right time window, which is defined by the balance between the reliability of data leading to proper decisions and the timely but successful treatment of the pest problem. Certain limitations of remote sensing techniques include atmospheric conditions, time scheduling and costs. However, this technology is rapidly evolved and will soon overcome present burdens. With the use of ground based sensors (multispectral sensors and RGB cameras), in Hydrosense project the opportunity of automating the collection of weed cover data was given, at a spatial resolution that is not economically feasible with manual sampling methods. Indeed, increasing the intensity of sampling results in a more accurate characterization of within-field variability. Towards increasing samples, the use of the multispectral sensor in relation to the camera is preferable because the user simply runs the selected scan area and his speed can refine the number of measurements. Capturing data with the camera, presupposes the choice of shooting points, going over to each one, positioning the camera system (tripod) and taking the picture. This process greatly increases measurement time and accordingly the user’s fatigue. To expedite shooting procedures, a video file could have been produced and further split into individual images or a corresponding aerial photograph could have been purchased. In terms of time, the number of records that a user can take with the multispectral sensor is higher than the according number of photos with the camera. Besides, increasing the number of records optimizes the spatial coverage of the interest area and thereby one can obtain a more representative picture of the weed flora in the region. Consequently, according to the work of the year 2012, the method of weed mapping using multispectral sensor is safer in terms of reliability, compared to taking photos. An aerial photo of the study area (e.g. by a camera mounted on a remote controlled airplane or helicopter) is expected to reduce time for raw data recording.Indeed these methods minimize measurement time and offer large scale results by capturing a large area with one shot, however they introduce increased costs and post processing office work. The purchase of equipment including the multispectral sensor, the data logger and the GPS, is quite expensive (according to current prices) and therefore unattractive to the average Greek producer. Instead, the camera system is cheaper and much easier to learn and use. Analysing data. Once data on weed spatial variability have been collected, it is necessary to establish a decision support tool to visualize the significant in field variation and to suggest the imminent management solutions. A means towards decision support in Precision Agriculture is Geographical Information Systems (GIS). GIS are systems, consisting of hardware and software, through which the user can acquire, store, retrieve, manage, analyse and map spatial and tabular data. What makes GIS a powerful decision support system is the expert knowledge that ought to integrate. Indeed, the integration of various data sources with expert knowledge and other decision models, can aid producers in taking strategic decisions for both short and long term. The cost of purchasing a GIS package is an issue for most Greek produces, and when this is overcome, the producer should prior be trained in how to use it and in fully developing its capabilities. A more realistic scenario under Greek farming conditions would be that of external servicing and agronomic consultation. Such services would undertake the data collection and analysis via GIS procedures, on behalf of the producer, and would be responsible for decision and strategic planning in farm and producer level. 39 Benaki Phytopathological Institute Upscaling data. The usage of weed data collected at field scale towards upscaling them at watershed level could be realized if a link between those sources of information was established. Under a reliable and substantiated link, which depends on the number of initial observations and their distribution in space, an upscaling through extrapolation procedure could be accomplished. More specifically, actions that could be undertaken through the upscaling of field data can be summarized as follows: 1. Defining the spectral signature of representative weeds, soils and crops in the pilot fields could be used to classify a satellite image covering the watershed area. As prerequisitesare the selection of unmixed and individual land cover types as reference areas, the verification of image classification results with field work and the determination of reliable and site specific reflectance value limits for each land cover. 2. The correlation of weed presence with soil and/or climate characteristics for which there is data availability at a watershed level, could serve as an indirect approach for defining wider weed flora zones, or risk zones susceptible to certain weed species infestations. 3. The information collected as part of the configuration of baseline status in the project’s area, through the deployment of questionnaires, is invaluable for decoding and depicting the importance of specific species grown in the area at a larger area. This could be strengthened by historical records found for the study area from secondary sources. Conclusions – Management decisions Weed management consists of a crucial and demanding agronomic process prior and during a cultivation period. Weeds among others factors can seriously diminish yield and degrade the quality of final product through competition phenomena, hosting and spreading diseases and enemies, preventing harvesting etc. Weed incidents are not homogenous across fields. In fact weed populations generally grow in patches; however producers tend to apply uniform rates of agrochemicals across their fields. This practice suggests unjustified use of pesticides and implies extra costs and environmental burdens. The key point in adapting and balancing inputs according to weed locations is the integrated monitoring of weed flora in terms of time and space. The methodology we used gave sufficient results as far as the management of weeds and insects in a cotton field are concerned. The insecticide methodology was based on the classic method of monitoring insects’ population through captures of pheromone traps. It constitutes a rather simple and efficient method. The advantages of this method are that the cost of trap establishment is low and no particular training of farmers is required. Finally it can ensure high effectiveness depressing the application cost. Generally, the specific conditions within each area and the presence of beneficial insects determine the balance between the insect population and level of damage on cotton production. In any case proper monitoring and timely communication of the observed situation would enable proper management decisions for pest control. Band applications of herbicides result in significant herbicide savings and provide chemical weed control only in the area where competition of weeds to the crop is necessary to be retained. In addition, herbicide applications using cutting-edge systems with sensors in the case of directed-post emergent applications with hoods, herbicide savings were ranged from 40-70%, which was accompanying with no significant reduction in cotton yield. However, the use of this method in a farmer’s level may have some difficulties. It is more than obvious that special training is required. To test effectively and to implement site-specific 40 Benaki Phytopathological Institute applications on a large scale, a sprayer suited for precisely targeting weed patches in the field is necessary. Given that cotton is susceptible to glyphosate applications over the top, the spraying system should have specially constructed hoods that retain the substance and therefore no phytotoxicity will be appeared. On the other hand the cost of such equipment is high for an average Greek cotton producer mainly because of the small farms (about 4 ha/farmer). However, an investment in a cooperative level could be a realistic solution. Parameters related to the development of regional GIS database Introduction The goal of this study was to detect correlations between field properties of the demonstration sites monitored during the growing season (ground truthing ) and radiance values of satellite imagery. These correlations need to apply not only within fields, but also across fields, in order to project them to the entire Pinios watershed and thereby provide any meaningful information to the GIS database. Exploratory correlation and factor analysis indicated that only few across field correlations were obtained between soil - plant variables and the wavebands of the satellite imagery. The lack of consistent correlations between any two variables across fields should not be surprising because of intrinsic variation in soil properties and management practices, i.e., different histories of fertilization and different soil types. Other interfering factors that made our goal difficult to achieve were a) the variable-rate inputs that altered field properties during the growing season and, b) the difficulty to exactly align the coordinates of canopy measurements of the multispectral sensors to those of the fixed sampling positions. The few significant across-field correlations and their importance in the GIS database are the subject of this report. Methodology Composite soil and plant samples from four sampling locations in each management zone were taken several times during the growing season and analyzed in the laboratory. The exact position of each sampling location was recorded with a GPS receiver that received differential signal (Omnistar BV, Leidschendam, NL) with an accuracy of <0.5 m. Standard soil quality analysis included water content (w/w), pH and electrical conductivity (EC) adjusted to a 1:1 soil-water ratio. Nitrate content was determined colorimetrically (FIAstar 5000 analyzer by Foss) in soil extracts of 1M potassium chloride. Carbonate content, as an estimate of inorganic C, was determined by using a Bernard calcimeter to measure the released CO2 after addition to soil of dilute HCl solution. Total nitrogen and total carbon content, as well as isotopic composition (δ15N, δ13C) of soil and leaf samples, were measured by an automated combustion elemental analyzer interfaced with a triple collector isotope ratio mass spectrometer (IRMS, PDZ Europa, Crewe, UK). Soil organic matter was determined by the Walkley-Black method of wet oxidation. Soil texture was determined by physical fractionation. A World View 2 multispectral image was taken on March 23, 2010 covered an area of 122 km2 that included the HydroSense demonstration sites. At that time the soils were already plowed to a depth of 30 cm with unknown soil water content. The four spectral bands (blue at 442-515 nm, green at 506-586 nm, red at 624-694 nm and NIR at 765-901 nm) of the image were processed at a spatial resolution of 2.5 m in multispectral mode (0.5 m in 41 Benaki Phytopathological Institute panchromatic mode). Radiance values of surface reflectance of the pixels corresponding to each sampling location were recorded after the data was corrected for solar irradiance and atmospheric effects. Factor analysis, linear correlation and regression were used to quantify the strength of the relationship of soil and plant properties with image radiance values of the four spectral bands. The Statistical Analysis System (SAS Institute, 1990) was used for all data analyses. Results and conclusions Several correlations were obtained between soil-plant variables and pixel radiance values of satellite imagery within fields, but much fewer across fields. The lack of consistent correlations between any two variables across fields should not be surprising because of intrinsic variation in soil properties and management practices, i.e., different histories of fertilization and different soil types. However, significant correlations were also obtained across fields between pixel radiance values of satellite imagery and soil N, C, electrical conductivity, water content and texture. The highest (negative) correlation was obtained between satellite NIR reflectance and soil N content (Fig. 17). Our results indicate that NIR reflectance of high resolution satellite imagery was not only able to quantify within-field variability in total soil N, but also acrossfield variability in a single regression equation. This equation was used to predict the spatial distribution of soil organic matter content across a large section of the Thessaly Plain (included in the GIS maps provided in Action 5) and to provide a decision support tool for site-specific management. It is expected that the importance of these maps will be verified in future case studies for soil quality modeling at the field and regional scale. 42 Benaki Phytopathological Institute 140 y = 184 - 0.35x + 2.2x10-4x2 R2 = 0.77 -1 Total soil N (mg kg ) 120 100 80 60 40 20 200 400 600 800 Radiance of NIR band Figure 17. The negative relationship between satellite NIR reflectance and total soil N at the 5-30 cm depth across the three fields (circle: Gyrtoni, square: Omorfohori, triangle: Eleftherio). 43 Benaki Phytopathological Institute References Adhikari K., Carre F.,Toth G., and Montanarella L., (2009). Site specific Land Management. General concepts and Applicatioions. (Ed). Luxembourg: Office for Official Publications of the European Communities Alam, M., and Trooien, T. P., (2001). Estimating reference evapotranspiration with an atmometer. Appl. Eng. Agric., 17~2!, 153–158. Al-Karadsheh, E., Sourell, H., & Krause, R. (2002). Precision irrigation: New strategy irrigation water management. Conference on International Research on Food Security, Natural Resource Management and Rural Development, Available at: http://www.tropentag.de/2002/abstracts/full/34.pdf. Accessed 12 march 2012. Ben-Dor E. and Banin A., (1995). Near infrared analysis (NIRA) as a simultaneously method to evaluate spectral featureless constituents in soils., Soil Science, 159:259-269. Blume, H. R., Kuder, L. J., Jantz, D. R., and Shaw, A. D., (1988). “Methods in determining crop water usage.” Proc., Planning Now for Irrigation and Drainage in the 21st Century, D. R. Hay, ed. ASCE, New York, 368–375. Broner, I. and Law, R.A.P. (1991). Evaluation of a modified atmometer for estimating reference ET. Irrigation Science 12, 21-26. Broner, I., and Law, R. A. , (1991). Evaluation of a modified atmometer for estimating reference ET. Irrig. Sci., 12, 21–26. Burman, R. D., Nixon, P. R., Wright, J. L., and Pruitt, W. O., (1983). Water requirements. Design and operation of farm Irrigation systems, M. E. Jensen, ed., ASAE, Monograph No. 3, St. Joseph, Mich. Cardenas-Lailhacar B., Dukes M.D., (2010). Precision of soil moisture sensor irrigation controllers under field conditions Agricultural Water Management 97: 666-672 Crookston, M. A., (1988). Canvas covered Bellani plate atmometer. Proc., Planning Now for Irrigation and Drainage in the 21st Century, D. R. Hay, ed., ASCE, New York, 716– 723 Doerge, T., (1998). Defining management zones for precision farming. Crop Insights 8, 21. Durrence, J.S., Perry, C.D., Vellidis, G., Thomas, D.L., Kvien, C.K., (1998). Evaluation of commercially available cotton yield monitors in Georgia field conditions, ASAE Paper No. 983106. ASAE, St. Joseph, MI. Ehler W.L. Idso SB, Jackson RD, Reginato RJ., (1978). Wheat canopy temperatures: Relation to plant water potential. Agron J. 70:251J 70:251 44 Benaki Phytopathological Institute Eldredge, E.P., C.C. Shock, and Stieber, T.D., (1993). Calibration of granular matrix sensors for irrigation management. Agronomy Journal 85:1228-1232. European Commission (2002). Implementation of Council Directive 91/676/EEC Concerning the Protection of Waters against Pollution Caused by Nitrates from Agricultural Sources. Office for Official Publications of the European Communities, Luxembourg Evans, R. G., Buchleiter, G. W., Sadler, E. J., King, B. A., & Harting, G. B., (2000). Control for precision irrigation with self-propelled systems. In R. G. Evans, B. L. Benham, & T. P. Trooien (Eds.), Proceedings of the 2000 ASAE 4th decennial national irrigation symposium, St. Joseph, Michigan, USA,pp. 322–331. Evans, R. G., Han, S., & Kroeger, M. W., (1996). Precision center pivot irrigation for efficient use of water and nitrogen. In P. C. Robert, R. H. Rust, & W. E. Larson (Eds.), Proceedings of the 3rd international conference on precision agriculture (pp. 75– 84). Madison, WI, USA: ASA/CSSA/SSSA. Fares, A. and Polyakov V., (2006). Advances in Crop Water Management Using Capacitive Water Sensors. In: Advances in Agronomy, D. Sparks (Editor). Elsevier, Amsterdam, Vol. 90, pp. 43-77. Ferre, P.A., and Topp, G.C., (2002). Time domain reflectometry, in Dane, J.H., and Topp, G.C., eds., Methods of soil analysis, Part 4--Physical methods: Soil Science Society of America Book Series No. 5, Soil Science Society of America, Madison, Wisconsin, p 434-446. Galanopoulos, K., Aggelopoulos, S., Kamenidou, I. and K. Mattas (2006). Assessing the effects of managerial and production practices in the efficiency of commercial pig farming. Agric. Syst. U.K., 88: 125-141. Gates, D.M., (1964). Leaf temperature and transpiration. Agron. J., 56: 273-277GA Geerts S., Raes, D., (2009).Deficit irrigation as an on-farm strategy to maximize crop water productivity in dry areas. A review. Agricultural Water Management, 96:1275-1284 Gibbons, G., (2000). Turning a farm art into science/an overview of precision farming. URL: http://www.precisionfarming.com. Gitelson A.A., Vina A., Ciganda V., Rundquist D.C. and Arkebauer T.J. 2005. Remote estimation of canopy chlorophyll content in crops. Geophysical Research Letters 32 L08403. Goumas, K., (2006). The irrigation in the plain of Thessaly: Consequences in groundwater and surface waters, Greek, Hydrotechnical Union Meeting February 2, 2006, Water Resources and Agriculture. Hellenic Ministry for the Environment, Physical Planning and Public Works (2003) “Regional plan for sustainable development in Thessaly region”, Athens, Greece. (in Greek) 45 Benaki Phytopathological Institute Hess, T., (1998). Evapotranspiration estimates for water balance scheduling in the UK. Univ. Rep., Silsoe College, Cranfield Univ., U.K. Hignett, C., and Evett, S., (2008b). Electrical Resistance Sensors for Soil Water Tension Estimates. In: Evett, S.R., L.K. Heng, P. Moutonnet, and M.L. Nguyen, editors. Field Estimation of Soil Water Content: A Practical Guide to Methods, Instrumentation, and Sensor Technology. IAEA-TCS-30. International Atomic Energy Agency, Vienna, Austria. ISSN 1018-5518. Holland K.H. and Schepers J.S. 2010. Derivation of a variable rate nitrogen application model for in-season fertilization of corn. Agronomu Journal 102:1415-1424. Hunsaker DJ, Pinter PJ Jr, Cai H., (2003). Alfalfa basal crop coefficients for FAO-56 procedures in the desert southwestern US. Trans ASAE 45(6):1799–1815 Idso, S.B., (1982). Non-water-stressed baselines: a key to measuring and. Agric. Meteorology 27, 59-70. Irmak, M.D. Dukes, Jacobs J.M., (2005). Using modified Bellani plate evapotranspiration gauges to estimate short canopy reference evapotranspiration J. Irrig. Drain. Eng. ASCE, 131 (2), pp. 164–175 Irrometer Company, Inc., (2009). WATERMARK Soil Moisture Sensor with Voltage Output – MODEL 200SS-V. #405. Riverside, CA. Available online at:http://www.irrometer.com/datasheets/405.pdf Jackson R.D., (1982).Canopy temperature and crop water stress. In Hillel, D.(ed.) Advances in irrigation, Vol.1.Academic Press,New York,London,pp.43-85 Jackson,R.D., Idso, S.B., Reginato, R.J. and Pinter, P.J., (1981). Canopy temperature as a crop water stress indicator. Water Resources Res. 17, 1133-1138 Jones H.G., (1990). Plant water relations and implications for irrigation scheduling Acta Hortic., 278, pp. 67–76 Kvien, C., & Pocknee, S., (2000). Introduction to management zones. University of Georgia, USA: National Environmental Sound Production Agriculture Laboratory (NESPAL), College of Agricultural and Environmental Science Livingston B.E., (1915). A modification of the Bellani porous plate atmometer. Science 41:872-874 Loukas, A., (2010). Surface water quantity and quality assessment in Pinios River, Thessaly, Greece, Desalination, 250:266–273. Loukas, A. and Vasiliades L., (2004). Probabilistic analysis of drought spatiotemporal characteristics in Thessaly region, Greece, Natural Hazards and Earth System Sciences (2004) 4: 719–731. 46 Benaki Phytopathological Institute Magliulo V., R. d’Andria, Rana, G., (2003). Use of the modified atmometer to estimate reference evapotranspiration in Mediterranean environments Agric. Water Manage., 63, pp. 1–14 Mahleras, A., Kontogianni, A. and Skourtos M., (2007). PINIOS RIVER BASIN - GREECE (Deliverable D34), Aqua Money, Project report, pp. 27. Accessed August 2011 in (http://www.aquamoney.org/sites/download/piniosgr.pdf) accessed August 2011 Maohua, W., (2001). Possible adoption of precision agriculture for development countries at the threshold of the new millennium. Comp. and Elect. in Agric. 30: 45-50 McBratney AB & Whelan BM., 1995. The null hypothesis of precision Agriculture. In: Precision Agriculture `99', eds JV Stafford, Shefeld Academic Press Shefeld pp 947957. Moran, M.S., Vidal, A., Troufleau, D., Inoue, Y., Qi, J., Clarke, T.R., Pinter, P.J. Jr., Mitchell, T., and Neale, C.M.U., (1997). Combining multi-frequency microwave and optical data for farm management. Remote Sensing of Environment, Vol. 61, pp. 96–109. National Research Council (1997). Precision Agriculture in the 21st Century Geospatial and Information Technologies in Crop Management. National Academy Press Washington, D.C. Nemenyi M., P. Mesterhazi, Z. Pecze, Stepan Z., (2003).The role of GIS and GPS in precision farming Computers and Electronics in Agriculture, 40 (1–3) (2003), pp. 45–55 O'Shaughnessy, S. A., and Evett, S. R., (2010). Canopy temperature based system effectively schedules and controls center pivot irrigation of cotton. Agric. Water Manage. 97:1310-1316Shaughnessy and Evet (2010) Padhi J, R.K. Misra Payero J.O., (2012). Estimation of soil water deficit in an irrigated cotton field with infrared thermography. Field Crops Research. 126: 45-55 Paltineanu, I.C. and Starr, J.L., (1997). Real-Time Soil Water Dynamics Using Multisensor Capacitance Probes: Laboratory Calibration. Soil Sci. SOCA. m. J. 61:1576-1585. Perry, C.D., Vellidis, G., Wells, N., Kvien, C., (2001). Simultaneous evaluation of multiple commercial yield monitors in Georgia,. In: Richter, D.A. (Ed.), Proceedings of the Beltwide Cotton Conference. National Cotton Council of America, Memphis, TN, pp. 328–339. Peterson, P. R., C. C. Sheaffer and Hall, M.H., (1992). Drought effects on perennial forage legume yield and quality. Agronomy Journal 84: 774-779. Polizos, S., Sofios, S. and Goumas K, (2006). Longitudinal changes in groundwater resources of the region of Thessaly and the impact on regional development and the environment, 9th Panhellenic Conference of Rural Economy, Athens, 2-4 November 2006 47 Benaki Phytopathological Institute Postel, 1999. Pillar of Sand: Can the Irrigation Miracle Last?. New York and London: Worldwatch/W.W. Norton, 1999 Reitz P. and Kutzbach. H.D., (1996). Investigations on a particular yield mapping system for combine harvesters. Computers and Electronics in Agriculture, 14: 137-150. Roades, J.P., Beck, A.D., Searcy, S.W., (2000). Cotton yield mapping: Texas experiences in 1999. In: Richter, D.A. (Ed.), Proceedings of the Beltwide Cotton Conference. National Cotton Council of America, Memphis, TN, pp. 404–408. Sadler, E. J., Evans, R. G., Stone, K. C., & Camp, C. R., (2005a). Opportunities for conservation with precision irrigation. Journal of Soil and Water Conservation, 60(6), 371–379. Sassenrath-Cole, G.F., Thomson, S.J., Williford, J.R., Hood, K.B., Thomasson, J.A., Williams, J., Woodard, D., (1999). Field testing of cotton yield monitors. In: Richter, D.A. (Ed.), Proceedings of the Beltwide Cotton Conference. National Cotton Council of America, Memphis, TN, pp. 364–366. Searcy, S.W., (1998). Evaluation of weighing flow-based cotton yield mapping techniques. In Proc of the Fourth International Conference on Precision Agriculture, 1151-1163. St. Paul, MN, 19-22 July. Shibusawa, S., 1998. “Precision Farming and Terra-Mechanics.”Paper presented at the 5th ISTVS Asia-Pacific Regional Conference in Korea, October 20–22. Shock, C.C., J.M. Barnum, and Seddigh. M., (1998). Calibration of Watermark soil moisture sensors for irrigation management, pp.139-146, Proceedings of the International Irrigation Show, San Diego, CA. Irrigation Association. Spaans, E.J.A., and Baker, J.M., (1992). Calibration of Watermark soil moisture sensors for soil matric potential and temperature. Plant and Soil 143:213-217. Stamatiadis S., Evangelou L., Tsadilas C., Tsitouras A., Chroni C., Tsadila E, Christofides P., Blanta A., Samaras V. and Dalezios N., (2011).Satellite NIR reflectance correlates to soil organic matter and carbonate content in three fields of the Thessaly Plain (central Greece). Proceedings of the international Conference of Soil and Plant analysis. Chania, Greece 6-10 June 2011 Starr, J.L. and Paltineanu, I.C. , (2002). Capacitance Devices. In:Methods of Soil Analysis, J.H. Dane and G.C. Topp (Ed). Soil Sci. SOCo.f Am., Medison, Wisconsin, pp. 463-474. Sui R., J.A.Thomasson., R.Mehrle., M.Dale., C.Perry and Rains, G., (2004). Mississippi cotton yield monitor:beta test for commercialization. Computers and Electronics in Agriculture 42: 149-160 Thomasson, J.A., Pennington, D.A., Pringle, H.C., Columbus, E.P., Thomson, S.J., Byler, R.K., (1999). Cotton mass flow measurement: experiments with two optical devices. Applied Engineering in Agriculture 15 (1), 11–17 48 Benaki Phytopathological Institute Thompson R.B., M. Gallardo, T. Agüera, L.C. Valdez, Fernández, M.D., (2006). Evaluation of the watermark sensor for use with drip irrigated vegetable crops. Irrig. Sci., 24, pp. 185–202 Thompson, S.J., and Armstrong. C.F., (1987). Calibration of the Watermark Model 200 soil moisture sensor. Applied Engineering in Agriculture 3:186-186. Thomson, S.J., Fisher, D.K., Sassenrath-Cole, G.F., (2002). Use of granular–matrix sensors, models, and evaporation measuring devices for monitoring cotton water use and soil water status in the Mississippi Delta. In: Proceedings of the 2002 Beltwide Cotton Conference, National Cotton Council, Memphis, TN, USA, pp. 623–637. Tóth, G., Montanarella, L. and Rusco, E., (2007). Threats to Soil Quality in Europe (Eds.) Luxembourg: Office for Official Publications of the European Communities Unlu M, Kanber R, Levent DK, Tekin S, Kapur B., (2010). Effect of deficit irrigation on the yield and yield components of drip irrigation cotton in Mediterranean environment. Agric. Water Manage., 98: 597-605. Wall, R. W., King, B. A., & McCann, I. R., (1996). Center-pivot irrigation system control and data communications network for real-time variable water application. In P. C. Robert, R. H. Rust, & W. E. Larson (Eds.), Proceedings of the 3rd international conference on precision agriculture (pp. 757–766). Madison, WI, USA: ASA/CSSA/SSSA. Wang M., (1999). Technology development in ‘precision agriculture’ and technological innovation in agricultural equipment W. Cui (Ed.), Digital Earth, China Environmenal Science Press, pp. 47–53 Wanjura D.F and Upchurch D.R., (2000). Canopy temperature characterization of corn and cotton water status. Trans. ASAE 43 (4): 867-875 Wanjura D.F., D.R. Upchurch, Mahan, J.R., (2006). Behavior of temperature-based water stress indicators in biotic-controlled irrigation Irrig. Sci., 24 (2006), pp. 223–232 Weiss M., (1996). PF and spatial economic analysis: research challenges and opportunities. American Journal of Agricultural and Applied Economics, 78 (1996), pp. 1275–1280 Whelan, B.M., McBratney, A.B., Boydell, B.C., (1997). The Impact of Precision Agriculture. Proceedings of the ABARE Outlook Conference, ‘The Future of Cropping in NW NSW’, Moree, UK, July 1997, p. 5. Wilcox JC., (1963). Effect of weather on evaporation from Bellani plates and evapotranspiration from lysimeters. Can J Plant Sci 43:1-11 Wilkerson, J.B., Kirby, J.S., Hart, W.E., Womac, A.R., (1994). Real-time cotton flow sensor, ASAE Paper No. 941054. ASAE, St. Joseph, MI. 49 Benaki Phytopathological Institute Wilkerson, J.B., Moody, F.H., Hart, W.E., (2002). Implementation and field evaluation of a cotton yield monitor. Applied Engineering in Agriculture 18 (2), 153–159 Wolak, F.J., Khalilian, A., Dodd, R.B., Han,Y.J.,Keshlkin, M., Lippert, R.M., Hair,W., (1999). Cotton yield monitor evaluation, South Carolina–year 2. In: Richter, D.A. (Ed.), Proceedings of the Beltwide Cotton Conference. National Cotton Council of America, Memphis, TN, pp. 361–364. Yazar A, Sezen SM, Sesveren S., (2002). LEPA and trickle irrigation of cotton in the Southeast Anatolia Project (GAP) area in Turkey. Agric. Water Manage. 54(3): 189-203 Zhang, N. M Wang and Wang N., (2002). Precision Agriculture – a worldwide overview. Computers and Electronics in Agriculture (36) 113-132 Zhang, X., L. Shi., X., Jia, G. Seielstad and Helgason G., (2010). Zone mapping application for precision farming: a decision support tool for variable rate application. Precision Agriculture.11:103-114. 50