Table S5: Kegg pathways and disease terms significantly enriched by target genes of differentially expressed miRNAs during E. coli or S. aureus challenge of MAC-T cells. miRNA
Bta-miR-30b-5p
Bta-miR-30c
Bta-let-7a-5p
Kegg pathway
Axon guidance
Neurotophin signaling pathway
Ubiquitin mediated proteolysis
Long term potentiation
Amytrophic lateral sclerosis
B cell receptor signaling pathway
Pathways in cancer (colorectal cancer, non-small cell lung cancer, prostate cancer)
Arrhythmogenic right ventricular cardiomyopathy
14
Regulation of actin cytoskeleton
Dilated cardiomyopathy
27
15
Hypertrophic cardiomyopathy
Aldosterone-regulated sodium reabsorption
Wnt signaling pathway
14
9
20
Glioma 11
SNARE interactions in vesicular transport 8
ErbB signaling pathway
Endocytosis
MAPK signaling pathway
T-cell receptor signaling pathway
13
21
28
14
Focal adhesion
Type II diabetes mellitus
MAPK signaling pathway
Chronic myeloid leukemia
Pathways in cancer (pancreatic cancer, colorectal cancer, bladder cancer, small cell lung cancer, prostate cancer, nonsmall cell lung cancer, thyroid cancer,
22
8
42
18
41
# genes
24
23
22
14
12
14
33
*P-Value (**Benjamini
P-value)
2.5E-5 (0.004)
4.1E-5 (0.003)
5.1E-4 (0.026)
7.5E-4(0.028)
9.4E-4 (0.028)
0.002 (0.048)
0.002 (0.042)
0.002 (0.041)
0.004 (0.070)
0.005 (0.068)
0.006 (0.080)
0.007 (0.084)
0.009 (0.100)
0.012 (0.120)
0.015 (0.150)
0.018 (0.160)
0.033 (0.260)
0.034 (0.240)
0.039 (0.250)
0.043 (0.260)
0.044 (0.260)
6.7E-8 (1.1E-5)
3.1E-6 (2.5E-4)
3.7E-5 (0.002)
Disease
Schizophrenia, bipolar disorder, autism, body mass, hearing loss
(deafness), Schizoaffective disorder, bipolar disorder, methamphetamine abuse, type 2 diabetes, diurnal preference, hypertension, longevity, weight gain, retinal vascular occlusion, insulin
Breast cancer , melanoma, bone mass, esophageal cancer, colon cancer, colorectal cancer, heart failure, prostate cancer, blood pressure, arterial hypertension, ovarian cancer, bone mineral

endometrical cancer)
Melanoma
Glioma
P53 signaling pathway
Neurotrophin signaling pathway
Hypertrophic cardiomyopathy
Adipocytokine signaling pathway
Focal adhesion
Axon guidance
ECM-receptor interaction
GnRH signaling pathway
TGF-beta signaling pathway
Heparan sulfate biosynthesis
Insulin signaling pathway
Cell cycle
Type II diabetes mellitus
Dilated cardiomyopathy
Lysosome
O-Glycan biosynthesis
T cell receptor signaling pathway
Progesterone-mediated oocyte maturation
Chondroitin sulfate biosynthesis
ErbB signaling pathways
Bta-miR193a-3p GnRH signaling pathway
Pathways in cancer (colorectal cancer,
Bta-miR423-5p renal cell carcinoma)
Neurotrophin signaling pathway
Wnt signaling pathway
Acute myeloid leukemia
Melanogenesis
MAPK signaling pathway
Oocyte meiosis
MAPK signaling pathway
8
8
5
6
10
6
18
5
11
8
14
8
12
14
6
12
13
12
6
16
15
13
11
15
14
14
17
13
11
22
16
1.2E-4 (0.005)
1.3E-4 (0.004)
2.9E-4 (0.005)
0.005 (0.079)
0.007 (0.092)
0.009 (0.110)
0.016 (0.160)
0.016 (0.160)
0.017 (0.170)
0.017 (0.170)
0.021 (0.160)
0.022 (0.160)
0.024 (0.160)
0.027 (0.180)
0.028 (0.170)
0.031 (0.180)
0.034 (0.200)
0.039 (0.390)
0.041 (0.210)
0.045 (0.220)
0.048 (0.220)
0.049 (0.220)
0.001 (0.140)
0.004 (0.180)
0.005 (0.170)
0.014 ()0.230
0.017 (0.240)
0.027 (0.310)
0.038 (0.350)
0.019 (0.340)
0.004 (0.390) density, bladder cancer, heart rate, resting, body mass (obesity, localised), cardiomyopathy (heart failure), periodontitis, metabolism disorders, longevity, chromosomal damage, multiple sclerosis, osteoarthritis, stomach cancer, chronic obstructive pulmonary disease, bone density (fracture, vertebral), type 2 diabetes
(insulin), lymphoma, preterm delivery, Alzheimer’s disease, liver transplant, hypertension, heart transplant
Alzheimer’s disease
Thyrotoxic periodic paralysis

Bta-miR-499
Bta-miR-99b
Bta-miR365-3p
Glycerophospholipid metabolism
Axon guidance
GnRH signaling pathway
Insulin signaling pathway
Calcium signaling pathway)
Pathways in cancer (colorectal, prostate, non-small cell lung cancer, renal cell carcinoma, pancreatic cancer, small cell lung cancer, endometrical cancer)
Neutrophin signaling pathway mTOR signaling pathway
Apoptosis
Wnt signaling pathway)
MAPK signaling pathway
Chemokine signaling pathway
Progesterone-mediated oocyte maturation
Glioma
Melanogenesis
Regulation of actin cytoskeleton
Long-term-potentiation
Melanoma
Chronic myeloid leukemia
B cell receptor signaling pathway
Vascular smooth muscle contraction
Jak-STAT signaling pathway
Cell cycle
Pathways in cancer (colorectal cancer, basal cell carcinoma)
Regulation of actin cytoskeleton
Axon guidance
Cell adhesion molecules
Oocyte meiosis
Pathways in cancer (colorectal cancer)
Long-term potentiation
8
11
9
10
11
14
9
6
7
9
12
9
6
8
6
6
4
5
3
5
6
9
5
5
5
5
6
7
6
11
0.005 (0.280)
0.007 (0.260)
0.011 (0.310)
0.025 (0.480)
0.048 (0.660)
0.000 (0.045)
0.001 (0.036)
0.001 (0.044)
0.003 (0.049)
0.004 (0.043)
0.003 (0.039)
0.009 (0.100)
0.022 (0.100)
0.017 (0.140)
0.020 (0.150)
0.020 (0.140)
0.023 (0.140)
0.026 (0.150)
0.031 (0.160)
0.031 (0.160)
0.032 (0.150)
0.036 (0.160)
0.048 (0.200)
0.021 (0.850)
0.038 (0.690)
0.041 (0.610)
0.044 (0.560)
0.003 (0.130)
0.010 (0.220)
0.014 (0.210)
-
-
Cognitive function depression

Bta-miR-24-3p
Bta-miR-148a
Progesterone-mediated oocyte maturation
Melanogenesis
Axon guidance
MAPK signaling pathway
Colorectal cancer
Circadian rhythm
Melanogenesis
3
Endocytosis
Neurotrophin signaling pathway
ErbB signaling pathway
TGF-beta signaling pathway
Regulation of actin cytoskeleton
Wnt signaling pathway
14
11
SNARE interactions in vesicular transport 5
Calcium signaling pathway 12
13
10
8
8
3
13
19
9
4
9
13
20
10
26
Focal adhesion
Focal adhesion
Glioma
Pathways in cancer (renal cell carcinoma, prostate cancer, pancreatic cancer, nonsmall cell lung cancer)
TGF-beta signaling pathway
Regulation of actin cytoskeleton
Hypertrophic cardiomyopathy
Melanoma
Wnt signaling pathway
ECM-receptor interaction
P53 signaling pathway
ErbB signaling pathway
Cell cycle
Acute myeloid leukemia
Chronic myeloid leukemia
Dilated cardiomyopathy
Axon guidance
13
9
8
9
11
11
18
10
9
7
8
9
11
0.023 (0.200)
0.029 (0.210)
0.002 (0.210)
0.005 (0.310)
0.009 (0.330)
0.009 ()0.380
0.022 (0.460)
0.027 (0.470)
0.029 (0.440)
0.032 (0.430)
0.032 (0.430)
0.037 (0.440)
0.038 (0.410)
0.042 (0.420)
0.044 (0.40)
0.048 (0.410)
2.5E-4 (0.031)
6.3E-4 (0.039)
7.6E-4 (0.032)
0.002 (0.055)
0.004 (0.090)
0.005 (0.100)
0.006 (0.087)
0.014 (0.160)
0.015 (0.160)
0.015 (0.150)
0.019 (0.160)
0.022 (0.170)
0.024 (0.170)
0.025 (0.170)
0.025 (0.160)
0.027 (0.170)
HIV, tuberculosis, cleft lip with cleft palate, cleft lip without cleft palate, arthritis, type 1 diabetes, recurrent pregnancy loss, paratyphoid feber, typhoid fever
Type 2 diabetes, cholesterol, atherosclerosis, endometriosis, height, juvenile arthritis,
Huntington disease, Alzheimer’s disease

Bta-miR-2339
Bta-miR-23a
Bta-miR21-3p
Bta-miR-486
Bta-miR-92a
Adipocytokine signaling pathway
Adherens junction
Axion guidance
Pathways in cancer (renal cell carcinoma, colorectal cancer, pancreatic cancer, prostate cancer)
Adherens junction
Tight junction
Focal adhesion
Regulation of actin cytoskeleton
Glycosphingolipid biosynthesis
MAPK signaling pathway
FC gamma R-mediated phagocytosis
Wnt signaling pathway
Chronic myeloid leukemia
Axon guidance
Chondroitin sulfate biosynthesis
Endocytosis
Neurotrophin signaling pathway
P53signaling pathway
Phosphatidylinositol signaling system
TGF-beta signaling pathway
-
Melanoma
Pathways in cancer (small cell lung cancer, prostate cancer)
P53 signaling pathway
Glioma
Axon guidance
Dilated cardiomyopathy
Hypertrophic cardiomyopathy
Phosphatidylinositol signaling system
Calcium signaling pathway
Regulation of actin cytoskeleton
Pantothenate and CoA biosynthesis
7
4
5
45
18
22
25
26
7
29
14
19
12
17
10
9
15
17
4
5
4
5
11
6
15
10
10
11
-
6
11
0.044 (0.250)
0.052 (0.990)
0.053 (0.910)
1.1E-6 (1.6E-4)
4.5E-6 (3.2E-4)
9.4E-5 (0.003)
0.002 (0.053)
0.002 (0.052)
0.004 (0.069)
0.006 (0.086)
0.007 (0.092)
0.007 (0.087)
0.007 (0.084)
0.007 (0.078)
0.011 (0.110)
0.025 (0.180)
0.027 (0.190)
0.044 (0.270)
0.049 (0.290)
-
8.6E-4 (0.066)
0.002 (0.078)
0.006 (0.140)
0.029 (0.440)
0.047 (0.540)
0.006 (0.570)
0.011 (0.520)
0.014 (0.480)
0.019 (0.490)
0.023 (0.470)
0.025 (0.440)
-
IgE, spinocerebellar ataxia,
Dupuytren’s disease, juvenile polyposis, cleft lip with cleft palate, cleft lip without cleft palate
-
Post-traumatic stress disorder,
Alzheimer’s disease, type 2 diabetes, autism, longevity, sporadic primary melanoma
Spinocerebellar ataxia

Bta-miR-184
Long-term potentiation
Ubiquitin mediated proteolysis
MAPK signaling pathway
Cell cycle
Chronic myeloid leukemia
8
12
19
11
3
0.027 (0.410)
0.034 (0.450)
0.039 (0.460)
0.043 (0.460)
0.059 (0.761) -
*P-Value: The significance of gene-term enrichment with a modified Fisher's Exact Test
**Benjamini P-Value: Globally corrects enrichment p-values in order to control family-wide false discovery rate.