Table S1. Strains and plasmids used in the studies.
Strain and plasmid
Relevant characteristics
Source or reference
EHA105
C58 strain containing pTiBo542 harboring the vir
genes but not T-DNA
(Hood et al. 1993)
EHA105ΔvirE2
EHA105 derivative, with virE2 deleted from
pTiBo542
This study
EHA105virE2::GFP11
EHA105 derivative, with the GFP11 coding
sequence inserted into virE2 on pTiBo542 at 162 bp This study
downstream from ATG+1
EHA105virE2::GFP11nls1
EHA105virE2::GFP11 derivative, with the first
nuclear localization signal residues,
221KLR. . .KYGRR237, replaced with alanine
A. tumefaciens
This study
A348
EHA105virE2::GFP11derivative, with virD4
deleted from pTiBo542
EHA105 derivative, with the GFP11 coding
sequence inserted at C-terminus of virE2 on
pTiBo542
A136 (pTiA6NC) (Octopine-type)
A348ΔvirE2
A348 derivative, with virE2 deleted from pTiA6NC This study
A348-105virE2
A348 derivative, with its original virE2 gene
replaced by EHA105 virE2
This study
A348-105virE2::GFP11
A348 derivative, with its original virE2 gene
replaced by EHA105 virE2::GFP11
This study
Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
Open Biosystems
pCMV-mGFP1-10
A vector containing GFP1-10 coding sequence
American Peptide Company
pmGFP Cterm S11
A vector containing GFP11 coding sequence
American Peptide Company
EHA105virE2::GFP11ΔvirD4
EHA105virE2-GFP11C
This study
This study
(Knauf and Nester 1982)
S. cerevisiae
BY4741
Plasmids
r
pCB301
A mini binary vector; Km
(Oliver et al. 1999)
pHB
A binary vector; pCAMBIA3301 derivative, with
GUS coding sequence replaced by htpII, encoding
hygromycin phosphotransferase II; an expression
(Mao et al. 2005)
cassette containing 2×35S promoter, MCS, and
RBCS poly(A) sequence, was cloned into EcoRI and
HindIII sites.
pHB-GFP1-10
pHB derivative, with the GFP1-10 coding sequence
This study
inserted at HindIII.
pQH04-GFP1-10
The GFP1-10 coding sequence was inserted at
HindIII to replace AD4 of pACT2 (Clontech), LEU2 This study
was replaced by HIS3
pHT101
A derivative of the binary vector pCB301, ligated at
SalI with pACT2, in which the GAL4AD gene is
This study
replaced at HindIII by the EGFP reporter gene
driven by the ADH1 promoter.
pEX18Tc
Counterselectable plasmid carrying sacB marker,
oriT; Tcr
pEX18TcKm
pEX18Tc derivative, with a 1029 bp nptIII cassette
This study
fragment inserted at KpnI, Kmr
pA348-VE2KO
pEX18TcKm derivative, with a 834 bp fragment
upstream of virE2 (from pTiA6NC) inserted at PstI
This study
and a 985 bp fragment downstream of virE2 (from
pTiA6NC) inserted at BamHI
pA348-105VE2
pA348-105VE2::GFP11
(Schweizer et al. 1998)
pA348-VE2KO derivative, with a 1650 bp EHA105
virE2 coding sequence inserted between the A348 This study
virE2 upstream and downstream sequence
pA348-VE2KO derivative, with a 1698 bp EHA105
virE2::GFP11 coding sequence inserted between the This study
A348 virE2 upstream and downstream sequence
pEHA105-VE2::GFP11
pEX18TcKm derivative, with a virE2::GFP11
fragment inserted at PstI
pEHA105-VE2-GFP11C
pEX18TcKm derivative, with a virE2-GFP11 coding
sequence together with a 1205 bp fragment
This study
downstream of virE2 inserted at PstI.
pQH308A
The GFP1-10 coding sequence was inserted into
pDs-Lox (Woody et al. 2007) to replace Bar. The
resulting cassette Pmas:GFP1-10:Tnos was PCR
amplified and inserted into pBI121 (Chen et al.
2003) at ClaI-HindIII. A DsRed ORF was then
inserted at XbaI-BamHI downstream of 35S
promoter.
A. thaliana
H16
N. benthamiana
Nb308A
This study
This study
Transgenic Arabidopsis thaliana plant with T-DNA
from pHB-GFP1-10
This study
Transgenic N. benthamiana plant with T-DNA from
pQH308A
This study
REFERENCES
Chen, P.Y., Wang, C.K., Soong, S.C. and To, K.Y. (2003) Complete sequence of the binary vector
pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breeding, 11,
287-293.
Hood, E.E., Gelvin, S.B., Melchers, L.S. and Hoekema, A. (1993) New Agrobacterium helper plasmids
for gene transfer to plants. Transgenic Res, 2, 208-218.
Knauf, V.C. and Nester, E.W. (1982) Wide host range cloning vectors: a cosmid clone bank of an
Agrobacterium Ti plasmid. Plasmid, 8, 45-54.
Mao, J., Zhang, Y.C., Sang, Y., Li, Q.H. and Yang, H.Q. (2005) A role for Arabidopsis cryptochromes
and COP1 in the regulation of stomatal opening. Proc Natl Acad Sci USA, 102, 12270-12275.
Oliver, D.J., Xiang, C.B., Han, P., Lutziger, I. and Wang, K. (1999) A mini binary vector series for plant
transformation. Plant Mol Biol, 40, 711-717.
Schweizer, H.P., Hoang, T.T., Karkhoff-Schweizer, R.R. and Kutchma, A.J. (1998) A broad-host-range
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