Standard Operating Procedure
Settlement Class: Peroxide Forming Chemicals, Class 2
Diethyl Ether
Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
Department:
Chemistry
Date SOP was written:
11/26/2012
Date SOP was approved by PI/lab supervisor:
11/27/2012
Principal Investigator:
Richmond Sarpong
Internal Lab Safety Coordinator/Lab Manager:
Rebecca Murphy
Lab Phone:
510-643-2485
Office Phone:
510-643-6312
Emergency Contact:
Richmond Sarpong 626-644-2407
(Name and Phone Number)
Latimer 834, 836, 837, 838, 842, 844, 847, 849, 907
Location(s) covered by this SOP:
(Building/Room Number)
Type of SOP:
Diethyl Ether
☐ Process
☒Hazardous Chemical
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SOP Template developed by The UC Center for Laboratory Safety
☐ Hazardous Class
Date: 1/10/2013
Purpose
Diethyl ether is a Peroxide Forming Chemical (PFC). It forms explosive mixtures with air. Ether peroxides are contact explosives when dry. Diethyl
ether is also extremely flammable. The auto-ignition temperature of diethyl ether is 160°C (320°F) and can therefore it can be ignited by a hot
surface without a flame or spark. If not stored and handled properly, this can pose a serious threat to the health and safety of laboratory personnel,
emergency responders and chemical waste handlers. Hence, it is important to follow safety protocols to handle this chemical.
Diethyl ether is a common laboratory solvent used in liquid-liquid extractions. It is particularly important as a solvent in the production of cellulose
plastics such as cellulose acetate.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 60-29-7
Class: Highly Flammable and Peroxide Former
Molecular Formula: C4H10O
Form (physical state): Liquid
Color: colorless
Boiling point: 34.6°C
Flash Point: -40oC (-40oF)
Potential Hazards/Toxicity
Prolonged storage of diethyl ether could lead to the formation of explosive peroxides. It has also been shown to have mutagen effects. Causes
skin, eye, and respiratory tract irritation. Inhalation of vapors may cause narcosis, nausea, loss of consciousness, dizziness and drowsiness. May
cause digestive tract irritation and central nervous effects through ingestion. Symptoms include headache, excitement, fatigue, nausea, vomiting,
stupor, and coma.
CalOSHA Permissible Exposure Limit (PEL): 400 ppm, 500 ppm STEL
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
Engineering Controls
NOTE: Lab-specific information on engineering controls may be included in the Protocol/Procedure section.
Work with diethyl ether should be conducted in a fume hood unless other controls are designated in the Protocol/Procedure section. Sash height
should be kept low to avoid escaping fumes and provide explosion barrier. Use supplemental explosion protective equipment like a blast shield
where appropriate to protect from explosions in the case of peroxide detonation
Personal Protective Equipment (PPE)
NOTE: Lab-specific information on PPE selection may be included in the Protocol/Procedure section.
Respiratory Protection
NOTE: Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S. This is a regulatory requirement.
Respirators should be used only under any of the following circumstances:
 As a last line of defense (i.e., after engineering and administrative controls have been exhausted).
 When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that PEL will be exceeded.
 Regulations require the use of a respirator.
 An employer requires the use of a respirator.
 There is potential for harmful exposure due to an atmospheric contaminant (in the absence of PEL)
 As PPE in the event of a chemical spill clean-up process
Hand Protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid
skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash
and dry hands. The recommended glove types for use with diethyl ether are laminate film, polyvinyl alcohol, neoprene and nitrile. If the possibility
exists for extended contact consider double gloving or wearing a thicker version of the selected glove material.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with diethyl ether
For more info, go to: http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection-and-usage.html
NOTE: Lab-specific information on glove selection may be included in the Protocol/Procedure section.
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
Eye Protection
Safety glasses with side shields or tightly fitting safety goggles. Use face shield (8-inch minimum) when appropriate. Use equipment for eye
protection tested and approved under appropriate government standards such as ANSI Z87-1, NIOSH (US) or EN 166(EU).
Skin and Body Protection
Long pants, closed-toed and closed-heeled shoes, cotton-based clothing/attire, and flame resistant lab coat must be worn for protecting against
chemical hazards
Hygiene Measures
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
First Aid Procedures
Notify supervisor and EH&S immediately. Follow up with a call to 510-642-9090 to report the incident.
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. Consult a physician.
In case of skin contact
Take off contaminated clothing immediately. Wash off with soap and plenty of water for 15 minutes. Take victim immediately to hospital. Consult a
physician.
In case of eye contact
Rinse thoroughly with plenty of water for at least 15 minutes using an emergency eyewash station, occasionally lifting the upper and lower eyelids.
Get medical aid immediately. Continue to wash eyes during transport to the hospital.
If swallowed
Do not induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.
Special Handling and Storage Requirements
NOTE: Laboratory-specific information on handling and storage may be included in the Protocol/Procedure section.
For additional information on handling and storage requirements refer to the EH&S Fact Sheet “Guidelines for the Safe Handling of Peroxidizable
Chemicals” (http://www.cchem.berkeley.edu/cchasp/sites/ehss/files/pdf/section7_insert17.pdf) and The UC Berkeley Standard operating
Procedure “Peroxide Forming Chemicals”.
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
Working alone Certain extremely hazardous operations should not be performed if the PI or Lab Safety Contact(s) are not present. Never work
alone with extremely hazardous materials/operations. See the Protocol/Procedure section below for specific prohibitions (if any) on working alone.
Precautions for safe handling Avoid contact with skin and eyes. Avoid inhalation of vapor or mist. Use explosion-proof equipment. Keep
away from sources of ignition - No smoking. Take measures to prevent the buildup of electrostatic charge. Be sure there are no white crystals
forming on the outside of the bottle. Work in an area with adequate ventilation. When handling the chemical, use it away from shock, friction, and
open flames. Prevent electric static build-up with a grounding cable. Wash thoroughly after handling.
Conditions for safe storage Please label the date received and the date opened, prior to working with diethyl ether. If there are white
crystals around the cap of the bottle, do not attempt to move the bottle and call EH&S immediately. The white crystals are a sign of explosive
peroxide formation and needs to be removed from the lab by a professionally trained person. Keep the chemical in a tightly sealed container in
dry and well-ventilated area. Containers of diethyl ether should be protected from physical damage, direct sunlight, and ignition sources. It should
be stored separately from strong oxidizing agents, strong acids, halogens, interhalogens, sulfur and sulfur compounds. Segregate the chemical
and store in secondary containment. Label the bottle, secondary containment, and storage cabinet as “Potentially Explosive Chemical.” Write the
date the chemical was received and date the chemical was opened.
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to
prevent leakage. Keep storage container closed (air tight) when not in use. Maintain the smallest amount necessary for ongoing work. Use in the
smallest practical quantities for the experiment being performed. Purchase diethyl ether with inhibitors added (for peroxide-forming) when
possible. All containers with diethyl ether should be labeled with a peroxide-testing label and stored in secondary containment. Test open diethyl
ether containers with peroxide test strips every 3 months.
An opened container can be stored for a maximum of 6 months. Dispose of unused amounts after that period of time has passed (or if peroxides
are found to be present by testing). Degassed diethyl ether may be stored in resealable Schlenk flasks under an atmosphere of argon or nitrogen,
or stored in closed containers in a glove box with a nitrogen or argon atmosphere
Spill and Accident Procedure
Chemical Spill Dial 911 and 510-642-9090
Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine
the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for
chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 and EH&S at 510-642-9090 for assistance.
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical
attention. Notify supervisor and EH&S immediately at 510-642-9090
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water from the emergency eyewash station for 15
minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S immediately at 510-642-9090.
Medical Emergency Dial 911
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 or go to the nearest emergency room.) Note: All serious
injuries must be reported to EH&S within 8 hours at510-642-9090.
Non-Life Threatening Emergency – Go to the Occupational Health Facility (OHF). After hours go to the nearest emergency room. Note: All
serious injuries must be reported to EH&S within 8 hours at 510-642-9090.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water
for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse \
and then enter your extension. After hours go to the nearest emergency room. Note: All needle stick/puncture exposures must be reported to
EH&S within 8 hours.
Decontamination/Waste Disposal Procedure
NOTE: Laboratory-specific information on decontamination/waste disposal may be included in the Protocol/Procedure section.
Wearing proper PPE, please decontaminate equipment and bench tops using soap and water. Please dispose of the used diethyl ether and
disposables contaminated with diethyl ether as hazardous waste.
General hazardous waste disposal guidelines:
Label Waste
 Label all containers with the label provided at http://ehs.berkeley.edu/hm/279-new-hazardous-waste-program-hwp.html. See the EH&S Fact
Sheet, “Hazardous Waste Management” for general instructions on procedures for disposing of hazardous waste.
Dispose of Waste


Dispose of regularly generated chemical waste within 6 months
Call EH&S for questions
Safety Data Sheet (SDS) Location
SDS can be accessed online at http://ucmsds.com
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
Preparation
Never open a dented or otherwise compromised container of diethyl ether. Alert lab safety manager
immediately.
Never open/use/move a diethyl ether container that is suspected to contain peroxides or is in contact with
crystals indicative of peroxide formation. Do not touch the crystals. Alert lab safety manager immediately.
Know the location of the nearest fire extinguisher, eyewash, and safety shower before beginning work.
Eliminate ignition sources such as open flames, hot surfaces, steam baths, and static electricity.
Lab-Specific
Information
When distilling diethyl ether appropriate precautions must be taken in case peroxides are present.
When distilling diethyl ether, care must be taken to insure the vapor is properly trapped.
Procedure/Use
Scale
Diethyl ether as a
solvent for
chemical reactions
Less than
1ml to 2
liters
Engineering
Controls/Equipment
All work using diethyl
ether should be carried
out inside a chemical
fume hood with proper
ventilation.
PPE (eye, face, gloves, clothing)
Procedure Steps and Special
Precautions for this Procedure
Eye Protection: Depending on
Use for reactions between -100oC and
o
the volume and hazardous nature 50 C.
of reagents, eye protection will
Insure than there are no ignition
vary from safety glasses with side
sources are present. This is particularly
shields to safety goggles (with or true when large volumes of ether are
without a face shield).
used.
Face Protection: As noted above
Never shake a container of ether.
a face shield may be necessary.
the container holding the solvent
Work is always done in a hood
should be capped to prevent
keeping the sash as low as
excessive evaporation of solvent.
possible or closed.
Hand Protection: The level of
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
As a peroxide forming chemical,
protection will be determined by
the hazards of the procedure.
The protection will vary from
nitrile (single or double layered),
polyvinyl alcohol, neoprene, or
laminate film.
Skin Protection: Depending o the
level of the experiment
protection will vary from long
pants, closed-toed and closedheeled shoes, cotton-based
clothing/attire, and flame resistant
lab coat must be worn for
protecting against chemical
hazards
Notes
Initials of
individuals using
this procedure
Any deviation from this SOP requires approval from PI.
NOTE
Diethyl Ether
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
diethyl ether should be stored for no
longer than 12 months after the date
of opening. All containers should be
dated with the date they are
received and opened. If any old
containers of diethyl ether test
positive for the presence of
peroxides or are suspected of
containing peroxides (due to the
presence of white crystals, etc.) they
should be tagged as hazardous
waste and disposed of properly.
Any deviation from this SOP requires approval from PI.
Documentation of Training (signature of all users is required)

Prior to conducting any work with diethyl ether, designated personnel must provide training to his/her laboratory personnel specific to the
hazards involved in working with this substance, work area decontamination, and emergency procedures.

The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the
manufacturer.

The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher
training within the last one year.
I have read and understand the content of this SOP:
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SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013
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Diethyl Ether
10
SOP Template developed by The UC Center for Laboratory Safety
Date: 1/10/2013