POST-DOCTORAL POSITION
GENOME ORGANIZATION AND ADVANCED
MICROSCOPY
Curie Institute, Paris
Laboratory: Nuclear dynamics, UMR 3664
Address: 26 rue d'Ulm, Paris 75005
Angela Taddei Team: Compartmentalization and dynamics of nuclear functions
Starting in January 2015
Summary of lab's interests :
The eukaryotic genome is packaged into large-scale chromatin structures that occupy
distinct domains in the nucleus. This DNA organization is a key contributor to genome
functions. Our aim is to understand what determines the spatial and temporal behavior of
chromatin and how this affects two essential functions of the genome: gene expression and
the maintenance of genome integrity. To understand these fundamental processes, we
combine genetics, molecular biology and advanced live microscopy.
Project:
In this project, we will investigate DNA organization using super resolution
microscopy in both budding yeast and human primary fibroblast cells. It was recently shown
that DNA mobility is increased in response to DNA double strand breaks. Here, we will use
super resolution microscopy (PALM/STORM) to directly observe the changes in DNA
organization following DNA damages.
The Taddei team provides all equipment’s necessary for yeast cells cultures and the
UMR3664 provides access to a cell culture room. A super resolution microscope is already
available in collaboration with the laboratory of Maxime Dahan and another super resolution
microscope is under construction in the UMR 3664. A spinning disk and a dual view
microscope are also devoted to yeast microscopy. The UMR 3664 of the Curie Institute is a
very stimulating environment to work in the epigenetics field and collaborations with
physicists leaders in microscopy is an important asset for the project.
The applicant should have experience in microscopy, and a strong interest in cell
biology. Experience in budding yeast and cell culture is appreciated. Organization,
responsibility and autonomy are required.
Please provide CV, names and emails of 2 referees to:
Judith.mine@curie.fr