) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of Science
Department
Biology
Full Name as written
in Passport
Shaymaa Fouad Rasheed Al-kaziragy
e-mail
Sh.fbio@yahoo.com
Career
Thesis Title
Year
Abstract
Assistant Lecturer
Lecturer
Assistant Professor
Professor
Master
PhD
3
The Effect of Glucan Binding Protein on Streptococcus mutans Biofilm Formation
and Determination of chemical Inhibitors
T
2010
hAbstract
e Fifty three isolates related to oral streptococci were obtained from one hundred
thirty three samples taken from patients with caries active. Thirteen isolates
E(24.52%) of total number of oral streptococci was identified as Streptococcus
mutans
while the rest isolates were related to species Streptococcus sobrins,
f
Streptococcus
sanguis, Streptococcus oralis, Streptococcus salivarus, and
f
eStreptococcus mitis which represented 18.86% ,15.09%,11.32% ,13.20%, and
c16.98% respectively.
t The ability of S. mutans isolates to produce slime layer was detected by using
tubes method and Congo red method. The results indicated that seven isolates
owhich represented 53.85% were slime layer producer by tubes method, while ten
isolates
which represented 76.92 % were slime layer producer by Congo red
f
method.
G The ability of S. mutans isolates to form biofilms was detected by using
microtiter
plates. The results indicated that all isolates have the ability to form
l
ubiofilms with different thickness degree and the absorbance values at 490nm are
cranged between (0.407-2.510). S7 isolate was the best biofilms producer isolate, its
aabsorbance value was (2.510).
n The sensitivity test against different antibiotics was done .It was found that S.
mutans isolates were sensitive to Amoxicillin, Cephalexin, Cephalothin,
BCiprofloxicin,
i
n
d
i
Cefixime, Erythromycin, Gentamycin, and Tetracyclin and
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
intermediated sensitive to Ampicillin while they showed resistance to Bacitracin.
The minimum inhibitory concentrations of antibiotics (Ampicillin,
Amoxicillin, Cephalexin and Erythromycin) to S. mutans isolates had been
determined. They were 1 µg/ml, 32 µg/ml, 16 µg/ml, and 64 µg/ml respectively
for the isolate S1 and they were 1 µg/ml, 64 µg/ml, 32 µg/ml, and 64 µg/ml
respectively for the isolate S7.
The minimum inhibitory concentrations of chemical inhibitors (Crest, Brushup, Stannous fluoride and Zak) to S. mutans isolates had been determined.
They were 0.01mg/ml, 0.023 mg/ml, 0.0125 mg/ml, and 0.0037 mg/ml
respectively for isolate S1, and they were 0.02 mg/ml, 0.023 mg/ml, 0.025
mg/ml, and 0.0075 mg/ml respectively for isolate S7.
The effect of sub minimum inhibitory concentrations of antibiotics
(Ampicillin,
Amoxicillin,Cephalexin,and
Erythromycin)on
biofilms
formation by S1 and S7 isolates in microtiter plate had been tested .The results
indicated that these concentrations of antibiotics inhibited bacterial adhesion
on polysteren surface of microtiter plates and consequently caused biofilms
detachment and this causes a decrease in absorbance values of biofilms.
The effect of sub minimum inhibitory concentrations of chemical
inhibitors (Crest, Brush-up,Stannous fluoride and Zak)on biofilms formation
by S1 and S7 isolates in microtiter plate had been tested . The results indicated
that the values of biofilms decreased after treatment with sub minimum
inhibitory concentrations of chemical inhibitors.
Glucan binding proteins (GBPs) were extracted and purified by using
affinity chromatography on Sephadex G-150 column with dimension of (1.2 x
10) cm and with flow rate (30ml/hr). The glucan binding proteins were eluted
from Sephadex G-150 by (3M) guanidine HCl.
Antibodies (Anti-GBPs) were prepared for this protein by injection this
protein in experimental animal. The titration of anti-glucan binding protein
(antibodies) for glucan binding protein of S. mutans in serum of rabbits
(control
and
experimental)
was
determined
by
using
passive
haemagglutination test. The results indicated that optimum concentration of
GBP was 50µg which gave positive reaction with highest dilution of serum of
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
1/320.
The effect of anti-GBP on biofilm formation by S. mutans was tested by
using microtiter plates. The results indicated that absorbance values of biofilm
decrease. Also results showed significant effect on biofilm formation after
treatment with dilution of anti-GBP. Experimental immunization with S.
mutans antigens involved in the adherence and accumulation of bacteria to
tooth surface (Gbp) have been shown to induce antibody response.