Abby pd. 4 10/4/11 West Branch River Study Statement of the Problem: What is the water quality of the West Branch of the Susquehanna River through Curwensville? Research: The land around the sampling site is between farmland and a city. Farmland water quality is usually good, and city water quality is usually poor. There is a bridge near the sampling site as well as a road. There are plenty of trees in the area. There are no large factories or industries near the site. Hypothesis: The water quality of the West Branch of the Susquehanna River across from the VFW in Curwensville, PA is good, but not excellent, based on the land use. The independent variable is the water quality. The dependent variable is the land use around the sampling site. Materials: Shoulder length gloves Waders Hand lenses Tweezers and spoons Large, shallow dish pans Plastic sorting tubs or ice cube trays Taxonomic key to macroinvertebrate life Calculator Kick net Abby pd. 4 10/4/11 Procedure: 1. Find the sampling site. There should be three different sites all in a 91 meter (or about 100 yard) area. The sites should be 41 meters (or about 45 yards) from any man-made objects. It is better if the site has riffles. 2. Start at the most downstream site so that the other sites are not biased by dislodging sediment or macro invertebrates. Select a one square meter riffle area. 3. Go upstream from the net holder about one meter and kick the first few inches of the streambed up for about one minute. The net holder should hold the net at a 45 degree angle to the water’s surface and firmly against the streambed. 4. The kicker grabs the bottom of the net and removes it with a forward scooping motion so that no macro invertebrates are carried away by the current when the net is removed. The net holder and the kicker carry the net to shore. 5. Sorters use spoons and tweezers to remove macro invertebrates from the net and sort them into containers to identify them. The macro invertebrates need to be sorted because some of them would harm others if they were in the same container. 6. Repeat the above steps at the other two sites. 7. Identify and record the types of macro invertebrates collected. 8. Gently release organisms upstream from where they were collected. Results: Macro Invertebrates found in West Branch of Susquehanna 60 Number found 50 40 30 20 10 0 Dobsonfly Larva Caddisfly Larva Other Mayfly Type of Macroinvertebrate Fingernail Clam Abby pd. 4 Type of Macro Invertebrate Dobsonfly Larva Caddisfly Larva Cranefly Larva Fingernail Clam Number found 8 51 20 6 Taxa 1 1 1 2 10/4/11 Relative Aubndance Rare Common Common Rare The location where the data was collected was in the West Branch of the Susquehanna River across from the VFW in Curwensville. The date was 9/26/11 and the time was 11:30 a.m. There were 19 participants in the experiment. The weather was mostly cloudy and the temperature was 26 degrees Celsius (79 degrees Fahrenheit). The water temperature was 19 degrees Celsius (66 degrees Fahrenheit) and the pH of the water was 6. Analysis: In the Group 1 Taxa there were 8 Dobsonflies collected, making them rare, 51 Caddisfly Larvae, making the common, and 20 other Mayflies, making them common. In the Group 2 Taxa there were 6 Fingernail Clams collected, making them rare. There were no organisms collected from the Group 3 Taxa. Most of the organisms collected were from the Group 1 Taxa, meaning they were the most sensitive. A wide array of organisms was not found; only a few types of organisms were found. Conclusion: The water quality is poor, but just barely. The water quality rating for the West Branch of the Susquehanna across from the VFW in Curwensville is 19.4, and the cutoff to be fair water quality is 20. So the water quality is on the upper end of poor. My hypothesis was wrong. I predicted that the water quality would be good, but it was poor. Therefore, the conclusion rejects my hypothesis. To improve my experiment, I could have tried to collect macro invertebrates on different days when the weather, water temperature, air temperature, etc. was different. Abby pd. 4 10/4/11 Through this experiment, I learned that you cannot base your hypothesis on just one factor. My hypothesis was based only on the land use, but the weather, temperatures, pH, time of day, and time of year might also have an effect on the water quality. Self Evaluation: 100/100