Optimization of protein extraction for differential proteomic analysis of the date palm infested with red palm weevil Khawaja Ghulam Rasool1, Abdulrahman S. Aldawood1, Muhammad Tufail1 and Makio Takeda2 1 Department of Plant Protection, King Saudi University, Riyadh, Saudi Arabia 2 Graduate School of Agricultural Science, Kobe University, Japan Abstract: The Kingdom of Saudi Arabia is one of the major producers of fine quality dates and ranks second in global dates production. This important fruit crop is under threat due to highly invasive pest, the red palm weevil (RPW) (Rhynchophorus ferrugineus). Failure in early detection of RPW infestation is the major obstacle in its successful management. In present study, we plan to identify the differentially expressed peptides of date palm possibly associated with red palm weevil infestation. Five protein extraction protocols with some modifications were used to extract protein from date palm leaves and stem. The objective of protein extraction protocol optimization was to select the best one with good protein yield in short time period. Therefore, yield and protein profiling were compared among the tested protocols. We observed that protocol with phenol/SDS extraction gave higher yield with reduced number of steps. Mini 2DE gels were used to assess reproducibility of protein profiling of infested and control protein samples. Both infested and control samples showed abundant proteins of different molecular weight/ sizes. Some protein spots were identified revealing variability in the intensity or relative abundance. The comparison of infested and control gels revealed that some protein spots were relatively up regulated. Also, some additional spots were identified in the infested samples. However, our future experiments with DIGE and Maldi TOF TOF will confirm the integrity of these differentially expressed peptides.