Jeffrey Nicholas Fisk 289-C Perkins Green |Rochester, NY 14623| (575)-644-9356| Email: jnf3769@rit.edu Education: Rochester Institute of Technology Rochester, NY June 2016 (expected) Major(s): BS in Biotechnology and Molecular Bioscience, BS in Bioinformatics, Combined MS in Bioinformatics| Minor: Spanish | Undergraduate/Graduate GPA: 3.84/4.0 on 4.0 scale Research: USC Wrigley Institute NSF Summer REU Fellow (2014): The Identification and Quantification of deep bacterial and archaeal communities at the San Pedro Ocean Time-series (SPOT) station using metagenomic and metatranscriptomic analyses Performed marine microbiology research under Dr. John Heidelberg and USC Graduate student Rohan Sachdeva. Identified and quantified bacterial and archaeal communities and their activity at 885meters below sea level. QIIME, mothur, Geneious, Bio-python, and R programs used. Cancer Informatics (2014-present): Searching for Oncogene targets for miRNA therapies Using TGDB, COSMIC, and miRBase, I have begun computationally looking for potential targets for synthetic microRNA therapies that present heightened specificity to the oncogenic form of the proto-oncogene. Note: This project is without mentorship. Bioinformatics Research (2013-present): A SNP-Dense Exon Approach to Identifying Paralogs in Humans. An extension of a unique project from my Bioinformatics course. Using Galaxy, NCBI, DGD, and USC Genome Browser, I have constructed a protocol for identifying potential paralogs in humans via gene functionality. Evolutionary Biology (2012): Geographic Reservoir Prediction and Phylogeny of Filoviruses Using NCBI, CodonCodeAligner, and a phylogenetic tree construction software, I reconstructed the evolutionary history of the filovirus family using bootstrap computational methods. After, I used the natural history of the virus and phylogenetic trees to make a prediction of the geographical origin of the common ancestor and a potential animal reservoir. Education/ Outreach: Biology Education Research (2012-present): Central Dogma Misconceptions and Their Origins Conducted education research aimed at identifying student misconceptions in the Central Dogma of Molecular Biology, recognizing potential sources thereof, and designing instructional tools and models for correcting them. DNA→RNA: What Do Students Think the Arrow Means? Published in CBE—Life Sciences Education Vol. 13, 338–348, Summer 2014 Phylogenetics and Cladistics Education (2013-present): Pokémon Phylogenetics: A educational exercise in cladistics Constructed a cladogram of over 300 of the fictional creatures Pokémon using categorical characters for instructional use. MEGA and Mesquite were used to develop the cladogram. Subsequently, the results were developed into a hands-on educational activity presented at ImagineRIT 2014. ImagineRIT Creativity and Innovation Festival Exhibitor (2012, 2013, 2014, 2015): 2012 How Genetics Works: Preformed a hands-on presentation about genetics and population genetics to layman community. 2013 CSI Debunked: The Truth about Forensics: Preformed a hands-on forensics presentation, including a simple HTML game, aimed at debunking common misconceptions about forensics. Was lead exhibitor and coordinator. 2014 CSI Debunked: The Truth about Fingerprints: Presented a hands-on activity about forensics, focusing on how fingerprinting and DNA fingerprinting work. Was lead exhibitor and coordinator. 2014 Pokémon Phylogenetics: Designed and presented a cladistics exercise geared towards teaching systematics to children. Was lead exhibitor and coordinator 2015 The Science of Superheroes: Designed and presented a series of interactive modules to engage people in scientific learning, using superheroes as case studies. Stress was put on communication in the scientific community. Was lead exhibitor and coordinator Celebrate Science and Mathematics (2014): Rochester Institute of Technology COS Presented an adapted version of the Pokémon Phylogenetics exhibit described above. Skills: Computational: Python, Java, MySQL, Terminal (Bash), R, C Bioinformatic: Galaxy, UCSC Genome Browser, Chimera, CodonCodeAlginer, NCBI, MEGA, Mesquite, mothur, QIIME, Bio-Python, Bio-Java, AutoDockVina, Geneious, FastQC, Tuxedo Package. Biotechnology: Blotting, Gel-Electrophoresis, Recombinant DNA techniques, Isolation of Microbes, PCR, RT-PCR, qPCR, Cell culture (CHO, HeLa, ect), hybridization techniques. Awards: Dean's List (Fall 2011-Fall 2015); Baldwin Wallace Memorial Scholarship (2013); Nathaniel Rochester Scholarship (2012, 2013), MG James Ursano Scholarship (2013, 2014), ThanksUSA Scholar (2014) Coursework: General Chemistry I-III, Organic Chemistry I-III, Biochemistry I&II, Genetics, Genetic Engineering, Bioinformatic Analysis of Macromolecules, Bioinformatics, Computer Science I&II, Molecular Modeling and Proteomics, Evolutionary Biology, Tissue Culture, Human Genetics, Microbiology, Databases and Data Modeling, Cell Biology, Molecular Biology, Cancer Biology, Mechanics of Programming (C), Bioinformatics Algorithms High-throughput Sequence Analysis, Genomics, Scientific Paper Writing, Bioinformatics Seminar, Bioinformatics Languages, Biological Separations, Genetic Diseases and Disorders, Concepts of Parallel and Distributed Systems, Statistical Models for Bioinformatics, Intermediate Spanish I-III, Medical Informatics Seminar, Ethics in Bioinformatics, Discrete Mathmatics, Physics I&II, Data Analysis Experience: Graduate Teaching Assistant: College of Science, Rochester Institute of Technology Fall 2014: Cell and Molecular Biology for Engineers I: Lab Instructor o Transformation, PCR, Electrophoresis, and other molecular biology techniques Spring 2015: Cell and Molecular Biology for Engineers II: Lab Instructor o Tissue Culture of Eukaryotic Cells Bioprep Technician:Bio-preparatory Lab at Rochester Institute of Technology:Fall 2012-present Producing and maintaining cell cultures aseptically (HeLa, CHO, ect) for use in course laboratories and research. Duty includes training successor. Preparing media and reagents using sterile technique for laboratory use Biological and chemical waste manifestation and disposal Interaction and communication with faculty concerning laboratory needs Publications: DNA→RNA: What Do Students Think the Arrow Means? L.Kate Wright, J. Nick Fisk, Dina Newman CBE—Life Sciences Education Vol. 13, 338–348, Summer 2014 Posters: 2014 USC Wrigley Institute for Environmental Studies NSF REU Research Symposium: “The Identification and Quantification of deep bacterial and archaeal communities at the San Pedro Ocean Time-series (SPOT) station using metagenomic and metatranscriptomic analyses” Nick Fisk, Rohan Sachdeva, Dr. John Heidelberg SABER 2014 “Development of a Central Dogma Concept Inventory (CDCI) for Use at All Levels of Undergraduate Biology” Dina L. Newman, J. Nick Fisk, Christopher Snyder, L. Kate Wright PKAL 2014“Development of a Central Dogma Concept Inventory (CDCI) for Use at All Levels of Undergraduate Biology” Dina L. Newman, J. Nick Fisk, Christopher Snyder, L. Kate Wright Professional Memberships: International Society for Computational Biology (2014-present) Systematics Association (2014-present) Activities: RIT Men's Division III Wrestling Team: (2011-Present): 3 Time Academic All-Conference Team Volunteer tutor at organized, weekly study sessions. Interim Captain Bioinformatics Journal Club: Public Relations Officer (2013-Present) Biology Education Research Group (2012-Present): Peer evaluation and Journal Studies Research Scholars (Biology) at RIT (2013-Present) Pokémon League at RIT (2014-Present)