Ly 3/2-3/6 Lesson Plan Ob jec tiv es Monday (A day) Tuesday (B day) Wednesday (A day) Thursday (B day) SWBAT: - Demonstrate an understanding of the process and purpose of gel electrophoresis -identify gender, and the absence or presence of a genetic mutation such as nondisjunction in meiosis and mitosis, monosomy (Turner Syndrome) and trisomy (Down Syndrome) from examples of karyotypes -describe genetic modifications such as the insertion of animal genes into bacterial plasmids for insulin production SWBAT: Demonstrate an understanding of the process and purpose of gel electrophoresis -identify gender, and the absence or presence of a genetic mutation such as nondisjunction in meiosis and mitosis, monosomy (Turner Syndrome) and trisomy (Down Syndrome) from examples of karyotypes -describe genetic modifications such as the insertion of animal genes into bacterial plasmids for insulin production SWBAT: -Recognize, describe, and sequence the stages of meiosis -Explain the importance of crossing over and how it contributes to the genetic variation of species -Evaluate the importance of two divisions to reduce chromosome number for sexual reproduction -Differentiate between gametes and somatic cells SWBAT: Demonstrate an understanding of the process and purpose of gel electrophoresis -identify gender, and the absence or presence of a genetic mutation such as nondisjunction in meiosis and mitosis, monosomy (Turner Syndrome) and trisomy (Down Syndrome) from examples of karyotypes -describe genetic modifications such as the insertion of animal genes into bacterial plasmids for insulin production ENGAGE: In what sorts of crimes would DNA fingerprinting help identify a criminal or clear a suspect? What kinds of samples would the forensic scientists need? SWBAT: -Recognize, describe, and sequence the stages of meiosis -Explain the importance of crossing over and how it contributes to the genetic variation of species -Evaluate the importance of two divisions to reduce chromosome number for sexual reproduction -Differentiate between gametes and somatic cells -Recognize, describe, and sequence the stages of meiosis -Explain the importance of crossing over and how it contributes to the genetic variation of species -Evaluate the importance of two divisions to reduce chromosome number for sexual reproduction -Differentiate between gametes and somatic cells -Recognize, describe, and sequence the stages of meiosis -Explain the importance of crossing over and how it contributes to the genetic variation of species -Evaluate the importance of two divisions to reduce chromosome number for sexual reproduction -Differentiate between gametes and somatic cells P Friday (A day) ENGAGE: How do you think forensic scientists are able to use blood samples to identify someone’s identity https://www.youtube.com/watch? v=AkBUriMK9u8 ENGAGE: Ask students where they got their traits from Do they look exactly the same as their parents or are they a combination of their parents’ traits? How much of your dad’s/ mom’s DNA do you have? ENGAGE: Review DNA fingerprinting -ask “what is the process/technique scientists use in order to obtain the unique DNA fingerprint?” For meiosis portion: Ask students where they got their traits from ENGAGE: Review stages of meiosis https://edpuzzle.com/media/54 f28d976a375a5a4961dea0 Page 1 of 3 Ly 3/2-3/6 Lesson Plan https://www.youtube.com/wat ch?v=AkBUriMK9u8 For meiosis portion: Ask students where they got their traits from Do they look exactly the same as their parents or are they a combination of their parents’ traits? How much of your dad’s/ mom’s DNA do you have? Do you have unique characteristics that neither of your parents have? L A EXPLORE: Gel Electrophoresis http://learn.genetics.utah.edu/ content/labs/gel/ DNA fingerprinting activity -Students work through the online activity and answer the questions on the worksheet Meiosis Crash Course video https://edpuzzle.com/media/5 4f262cf0b3b410d3a1c84da EXPLAIN: Students explain how DNA can be used to identify individuals using DNA fingerprinting and Gel electrophoresis. In what sorts of crimes would DNA fingerprinting help identify a criminal or clear a suspect? What kinds of samples would the forensic scientists need? Do you have unique characteristics that neither of your parents have? How is it that even though we all have half of our parents’ DNA, we still manage to look different from them? Ask students to raise their hands if they have siblings and ask these students if they look like their siblings. How is it that even though you and your siblings look different unless you are identical twins? EXPLORE: DNA fingerprinting activity -Students work through the online activity and answer the questions on the worksheet EXPLAIN: *show portion of DNA technology notes to introduce both DNA fingerprinting and Gel electrophoresis after the engage Students explain how DNA can be used to identify individuals using DNA fingerprinting and Gel electrophoresis. Students explain how they were able to identify the parents (in DNA fingerprinting activity) by comparing the bands of the parents and children Do they look exactly the same as their parents or are they a combination of their parents’ traits? How much of your dad’s/ mom’s DNA do you have? Do you have unique characteristics that neither of your parents have? How is it that even though we all have half of our parents’ DNA, we still manage to look different from them? Ask students to raise their hands if they have siblings and ask these students if they look like their siblings. How is it that even though you and your siblings look different unless you are identical twins? EXPLORE: Start Meiosis Matching Lab/Activity -students use textbook as a resource to match specific events with the different phases of meiosis EXPLORE: Gel Electrophoresis online activity http://learn.genetics.utah.edu/co ntent/labs/gel/ -students go through online simulation of gel electrophoresis EXPLAIN: Short review on mitosis before starting meiosis Meiosis Crash Course video https://edpuzzle.com/media/54f2 62cf0b3b410d3a1c84da Meiosis Sequencing Notes EXPLAIN: Students explain how DNA can be used to identify individuals using DNA fingerprinting and Gel electrophoresis. EXPLORE: Finish Meiosis Matching Lab -students use textbook as a resource to match specific events with the different phases of meiosis EXPLAIN: Meiosis Crash Course video https://edpuzzle.com/media/54f 262cf0b3b410d3a1c84da ELABORATE: Mitosis vs Meiosis Venn Diagram ELABORATE: Expand on how cross over and homologous recombination can lead to advantageous mutations in survival of the fittest Students explain how smaller DNA fragments travel down the gel faster than the larger pieces in gel electrophoresis. Page 2 of 3 Ly 3/2-3/6 Lesson Plan Students explain how smaller DNA fragments travel down the gel faster than the larger pieces in gel electrophoresis. Students explain how they were able to identify the parents (in DNA fingerprinting activity) by comparing the bands of the parents and children ELABORATE: DNA fingerprinting can be used to identify the culprit of a crime if the person left behind a sample of DNA at the scene of the crime DNA fingerprinting is not limited to crime scene evidence, it can also be used in wildlife conservation as well- to help identify which elephant herd illegal ivory trade is originating from. N Re sou rce s EVALUATE/ASSESS: Students turn in worksheets from both assignments -How are DNA fingerprinting and gel electrophoresis related to each other? -Students compare the similarities and differences between mitosis and meiosis ELABORATE: DNA fingerprinting can be used to identify the culprit of a crime if the person left behind a sample of DNA at the scene of the crime DNA fingerprinting is not limited to crime scene evidence, it can also be used in wildlife conservation as well- to help identify which elephant herd illegal ivory trade is originating from. https://www.youtube.com/watch ?v=toWK0fIyFlY Short review on mitosis before starting meiosis Meiosis Sequencing Notes Meiosis Crash Course video https://edpuzzle.com/media/54f2 62cf0b3b410d3a1c84da ELABORATE: DNA fingerprinting can be used to identify the culprit of a crime if the person left behind a sample of DNA at the scene of the crime DNA fingerprinting is not limited to crime scene evidence, it can also be used in wildlife conservation as well- to help identify which elephant herd illegal ivory trade is originating from. **So if you were to have a blood sample from a crime scene that contains DNA from two separate individuals, how might you identify the identity of these individuals? How many different DNA samples would you need to obtain? EVALUATE/ASSESS: Students turn in worksheets from assignments Mutations Quiz (first thing) Students explain how they were able to identify the parents (in DNA fingerprinting activity) by comparing the bands of the parents and children EVALUATE/ASSESS: Exit Ticket: What are the main differences between mitosis and meiosis? What is the main purpose of meiosis? What is the main purpose of mitosis? EVALUATE/ASSESS: Students turn in worksheets from assignments EVALUATE/ASSESS: Meiosis Quiz Page 3 of 3