1. Transcription begins at a RNA polymerase-binding site called a _____.
A. ribosome
B. replicator
C. inducer
D. promoter
2. In Eukaryotes, following transcription, the intron sequences are cut out of the primary
transcript and the ends are joined again prior to its use in protein synthesis. This processing of
the RNA is called RNA __________.
A. modification
B. translation
C. splicing
D. transcription
3. Which of the following is not true about eukaryotic DNA?
A. It is an exceedingly long and fragile molecule.
B. It is packaged into successively compact formations.
C. The entire molecule has encoded information for protein synthesis.
D. In the condensed form, it is transcriptionally inactive.
E. It must be unpackaged before it can be transcribed into RNA.
4. The "one gene-one enzyme" hypothesis was proposed by
A. Watson and Crick.
B. Griffith.
C. Garrod.
D. Franklin.
E. Beadle and Tatum.
5. Amino acids are transported to the ribosome for use in building the polypeptide by
A. mRNA molecules.
B. tRNA molecules.
C. DNA polymerase molecules.
D. rRNA molecules.
E. DNA ligase molecules.
6. You are conducting a genetic screen to isolate nutritional mutants in yeast. Specifically, you
want to isolate a histidine leucine double mutant. You start with a wild type yeast strain and
mutagenize it with a UV light. Which of the following outlines the remaining steps for isolating
such a mutant?
A. Grow mutagenized yeast on rich media. Grow yeast on minimal media, as well as on minimal
media containing histidine, minimal media containing leucine, and minimal media containing
both histidine and leucine. Select for yeast that do not grow on minimal media, do not grow on
media supplemented with only histidine or leucine, but do grow on minimal media supplemented
with both histidine and leucine.
B. Grow mutagenized yeast on minimal media. Grow yeast on rich media, as well as on minimal
media containing histidine, minimal media containing leucine, and minimal media containing
both histidine and leucine. Select for yeast that do not grow on rich media, do not grow on media
supplemented with only histidine or leucine, but do grow on minimal media supplemented with
both histidine and leucine.
C. Grow mutagenized yeast on rich media. Grow yeast on minimal media, as well as on minimal
media containing histidine, minimal media containing leucine, and minimal media containing
both histidine and leucine. Select for yeast that grow on minimal media, grow on media
supplemented with only histidine or leucine, but do not grow on minimal media supplemented
with both histidine and leucine.
D. Grow mutagenized yeast on minimal media. Grow yeast on rich media, as well as on minimal
media containing histidine, minimal media containing leucine, and minimal media containing
both histidine and leucine. Select for yeast that grow on rich media, grow on media
supplemented with only histidine or leucine, but do not grow on minimal media supplemented
with both histidine and leucine.
7. Given the sentence "THE FAT CAT ATE THE RED RAT," which of the following would
represent a frameshift mutation?
A. THE FAT CAT ATE THE RED RAT
B. THE CAT ATE THE RED RAT
C. THE FAC ATA TET HER EDR AT
D. THE FAT RAT ATE THE RED RAT
8. Base “wobble” refers to:
A. alternative RNA splicing
B. instability of the DNA molecule
C. the ability of the same tRNA anticodon to recognize different codons
D. the high mutability of certain genes
9. If you wanted to block transcription of a group of functionally related genes in a prokaryote,
you could place an obstacle
A. upstream of both the promoter and operon.
B. downstream of both the promoter and operon.
C. place an obstacle downstream of the promoter, but upstream of the operon.
D. place an obstacle upstream of the promoter, but downstream of the operon.