Part A – Review Answers Write as many different definitions as you can for each term listed below and make sure that they make sense to you. There is no sense in copying down a definition you do not understand. Stimulus, Catabolism, anabolism, metabolism, photosynthesis, cellular respiration, fermentation, anaerobic, aerobic, prokayote, eukaryote, monera, protista, plantae, fungi, animalia, endosymbiotic hypothesis producer, consumer, decomposer, autotroph, heterotroph, all the cell organelles, diffusion, osmosis, passive transport facilitated transport active transport, concentration gradient, KMT, hypertonic, hypotonic, isotonic, equilibrium, homeostasis, DNA, double helix, anti-parallel, hydrogen bond, phosphodiester bond, sugar phosphate backbone, nucleotide, nitrogenous base, purine, pyrimidine, base pairs, adenine, guanine, cytosine, thymine, uracil, RNA, complimentary mRNA sequence, tRNA, rRNA, DNA replication, semi-conservative, conservative, dispersive, leading strand, lagging strand, Messelson-Stahl Experiment, transcription, translation, codon, anti-codon, amino-acid, protein, enzyme/catalyst, mitosis, meiosis, diploid, haploid, ex. Decomposer – saprophyte, fungi and some monera and protista, heterotroph, breaks down dead organic matter for nutrients, digests before ingestions, important base in every ecosystem because they help recycle nutrients Using the word list above, match each definition with its term. Check the key when you finish group of organisms that are saprophytic heterotrophs _____Fungi______________________ term used to describe a lower solute concentration _______hypotonic________________________ an organism that depends on oxygen for the chemical reaction is uses to produce energy ________aerobic_______________________ the nitrogenous base that is a pyrimidine and base pairs with Adenine______thymine_________________________ cell process that occurs during interphase of its life-cycle in preparation for cell division _______DNA replication________________________ scientific theory that states that all molecules regardless of their state are in constant motion _______Kinetic Molecular Theory______ one example of an anabolistic reaction that autotrophs use ______photosynthesis_________________________ name the strand that because DNA strands are anti-parallel to one another takes longer to replicate ______lagging strand_______________ cells that do not contain homologous pairs of chromosomes ______haploid_________________________ theory that explains how the first eukaryotic cells evolved________endosymbiont hypothesis_______________________ only organelle other than the cell membrane that all prokaryotes have _______ribosome________________________ Organisms that are anaerobic often use this catabolistic reaction to produce energy _____fermentation__________________________ Describes the movement of water caused by the concentration gradients of solutes______osmosis_________________________ Process by which your blood cells are constantly being replaced ______mitosis_________________________ Type of RNA that has a binding site for both an amino acid and codon _____tRNA__________________________ Most primitive group of organisms that now has been split into two kindgoms and their own domain ____Monera_____________________ type of bond that ensures that the sequence of nucleotides is protected in both DNA and RNA ____phosphodiester bonds__________ theory of DNA replication eliminated after the first generation in the Messelson-Stahl experiment ______conservative_________________ would cause a cell to swell and possibly burst ____ hypotonic _____ solution A little Harder Now: Try making sentences using as many of the terms as you can but in their correct definition OR groups words together (2, 3, 4, or more) and explain their connection to one another. Ex. Photosyntesis and Cellular Respiration are both part of the metabolism of plantae which are autotrophic organisms that produce energy by an anabolistic reaction first to produce glucose and then a catabolistic reaction to release the energy in the form of ATP Making Connections: 1. Why does equilibrium not necessarily mean homeostasis? This is especially true for single celled organisms such as protists and monerans because in a multicellular organism they are able to better maintain the environment (solute concentration) of the cells within the organism. When a cell however if not in an isotonic environment, diffusion will likely occur and the cell/organism may experience nonideal gain or loss of solutes/solvents as a result until equilibrium is reached which may not be homeostatic for that cell/organism. 2. What are the two steps that ALL organisms need to satisfy in order for cells to have energy and which step so photosynthesis and cellular respiration fit into. In order for all living things to gain ATP energy for their cell to stay alive and function, they must first obtain an energy rich molecule and then they must release its energy through either an anaerobic or aerobic chemical reaction. Many organisms are heterotrophs and obtain the energy rich molecules through consuming them or decomposition however aututrophs make the molecules through chemical reactions. One such reaction is photosynthesis which produces glucose. Cellular respiration is the most common aerobic reaction used by living organisms that breaks down glucose releasing ATP energy as well as the waste products carbon dioxide and water. 3. The 5 Kingdom system that we learned at the start of the year has since evolved into a 6 Kingdom system and then later into a 3 Domain system. What was the basis for each system and the reasons for the changes In the 5 Kingdom system all monera are placed into the one Kingdom. Recent understanding of the diversity of Monera and their biochemical differences resulted in separating the Kindgom in to two, Archeabacteria(ancient bacteria) and Eubacteria(true bacteria) thus creating the 6 Kingdom system. We now know that the two have distinct and different evolutionary histories. Notice the branches in the cladogram above. 4. There are 5 basic functions of all living organisms. List them. Obtain and use energy, reproduce, growth and development, respond to stimuli, are made up to one or more cells. 5. Do you know your organelles? I suggest trying Quizlet or some other online quiz system. Set up an account for free and try a quiz or two to test yourself. https://quizlet.com/414811/cell-organelles-flash-cards/ 6. It is important that you understand the monomers and polymers of DNA, RNA, Proteins, Chromosomes. DNA is the double stranded polymer built with the monomer nucleotides. Each nucleotide consists of a phosphate group and deoxyribose sugar and a nitrogen base. RNA is a single stranded polymer built with monomer nucleotides also but the sugar is ribose instead and the nitrogen base T is U. Proteins are the polymer made from amino-acid monomers. There are 20 different amino acids which we aquire through ingestion. We don’t make them. Chromosomes are made up of DNA that is supercoiled and sections of the DNA are classed genes, which are the codes for specific proteins. 7. Why does the mRNA sequence even exist if the DNA and tRNA sequences are identical other than the U replacing T? mRNA is the messanger between the nucleus and the cytoplasm. DNA never leaves the nucleus but Protein synthesis occurs in the cytoplasm outside the nucleus thus the information must be carried from one to the other. Complimentary base pairing enables this process because the bases have a natural affinity for one another allowing for the “copying” of the message in its complimentary form. 8. DNA replication is very important why? And what does this process look like for Chromosomes DNA replication is essential because part of the definition of living things is that they are able to reproduce. In order for cells to divide they must contain two complete copies of their entire DNA to pass on to the new cells. During Interphase of a cell cycle the DNA replicates completely and then proofreads its work. This process transforms a chromosome consisting of one copy of the DNA into a chromosome that contains two copies in the form of attached sister chromatids. They still act and are considered a single chromosome. 9. How is it that homologous pairs of chromosomes have the same genes but can have different alleles. A set of homologous chromosomes refers to a diploid cell and chromosomes of the same chromosome number but each was inherited from a different parent. This means that although the pair of chromosomes contain all the same genes in all the same locations, since they were inherited from different parents, they may contain different versions of the genes which are called the alleles of the genes. Alleles are versions of genes that exist in a population as the result of mutations and natural selection over time. They are codes for different proteins of the same gene (function). 10. Can sister chromatids have different alleles? Explain Sister chromatids should not have different alleles since they are the product of DNA replication and if no errors are made in the process, then they should be identical. However is errors are made, this could be the source of new alleles in a population and it is possible however unlikely since only 1 mutation per three replications typically occurs. 11. Make up the transcribed side of a DNA sequence that is code for 5 amino acids. Determine its correct mRNA, tRNA and amino acid sequences. TTA ACC GCG CCC ATC AAU UGG CGC GGG UAG UUA ACC GCG CCC AUC Asn Trp Arg Gly stop DNA mRNA tRNA amino acids 12. Show why an substitution mutation may not result in a protein mutation, but a deletion or insertion mutation will. Insertions or deletion mutations shift all of the codons over or back by one base thus changing all of the codons after that point in the sequence and potential the rest of the amino acids in the chain whereas a substitution mutation simply replaces one base with another which may not result in any change to the protein since there is more that one codon sequence for many of the amino acids.