NINDS JHU Center for Neuroscience Research
Monoclonal Antibody Core Production Request Form
(Complete entire form and forward to Core Manager, Min Dai, mdai1@jhmi.edu)
(Prior to submitting an application, all users MUST read our document describing Important User Information,
located on our website at neuroscience.jhu.edu/resources/research-facilities/antibody-core
Applicant: Click here to enter text.
Building & Room No:Click here to enter text.
Phone:Click here to enter text.
E-mail: Click here to enter text.
Applicant PI:Click here to enter text.
Building & Room No: Click here to enter text.
Phone: Click here to enter text.
E-mail: Click here to enter text.
--------------------------------------------------------------------------------------------------------------------------------------------------Project Rationale: Click here to enter text.
NINDS* Grant # /Title: Click here to enter text.
*or other funding source
Specific Aim(s) of grant addressed by project: Click here to enter text.
--------------------------------------------------------------------------------------------------------------------------------------------------Antigen Name: Click here to enter text.
Antigen Form: Click here to enter text.
Molecular Weight: Click here to enter text.
Antigen Concentration: Click here to enter text.
Reagent/Materials in Sample: Click here to enter text.
Hybridoma
Production
Prefusion
Fusion
Cloning
mAb production:
Ascites production
mAb production:
Integra system for
production of mAb
mAb production:
cell culture method
Cell freezing
Check
if
Desired Service Description
Immunization of four (4) mice (Balb/c) and serum screening (approx.
6 to 10 weeks)
One or two mice (titer ³1:10,000) are selected for the fusion. Primary
screening is done by ELISA; confirmatory screening can be user’s
particular application (approx. 2-3 weeks)
Two of most promising hybridomas will be selected and cloned
(appprox. 2-3 weeks)
10 mice will be injected (approx. 4 weeks)
CL 350or CL 1000 flask will be used (concentration approx 1 –2
mg/ml) (approx. 4 weeks)
50 ml of antibody containing supernatant will be collected
(concentration ~50 ug/ml) (approx.. 4 weeks)
6 Vials will be delivered
--------------------------------------------------------------------------------------------------------------------------------------------------NINDS Center Terms of Use:
Because the Monoclonal Antibody Core is supported by NINDS, all publications involving work done in the
Core must comply with the NIH Public Access Policy (see publicaccess.nih.gov). It is the investigator’s
responsibility to ensure compliance.
Investigators are required to cite the Core (NS050274) in the Acknowledgments section of any publication that
results from use of Monoclonal Antibody Core.
Investigators are required to inform the Core Manager, Min Dai, when a paper which makes use of the facility
is accepted for publication.
Investigators are encouraged to inform the Core Manager, Min Dai, when an antibody generated by the Core is
shared with another lab. It is the applying lab’s responsibility to maintain records which allow tracking of
reagent sharing.
Pursuant to NIH policy, antibodies generated by the Monoclonal Antibody Core must be shared with the
scientific community once initial publication has occurred.
Applicant: Please enter name and date below to indicate acceptance of terms outlined above.
Applicant: Click here to enter text.
Date: Click here to enter text.
Laboratory PI: Please enter name and date below to indicate approval of the Application for Service
and acceptance of terms outlined above.
Approved by Lab PI: Click here to enter text.
Date: Click here to enter text.
--------------------------------------------------------------------------------------------------------------------------------------------------Monoclonal Antibody Core Facility Contact Information
Core Manager/Investigator Contact: Min Dai, 4-2077, mdai1@jhmi.edu, 911 Hunterian Bldg.
Core Director: Rick Huganir, PhD, 5-4050, rhuganir@jhmi.edu
Center Director: Alex Kolodkin, PhD, 4-9499, kolodkin@jhmi.edu
--------------------------------------------------------------------------------------------------------------------------------------------------For internal use only:
Approved by Core Manager: Click here to enter text.
Date:Click here to enter text.
Approved by Core Director: Click here to enter text.
Date: Click here to enter text.
Category: Center Investigator☐
Other NINDS Investigator☐
Non-NINDS Investigator☐