Quick Blue = Rapid Coomassie Post-Stain
For complex mixtures: lysates, serum etc
Real Time Blue = Coomassie Stain + Sample Loading Buffer + Pre-stained Protein Ladder
Engineered for simpler samples: partially purified proteins, protein fragments, FPLC fractions etc
This questionnaire allows us to determine which stain is best suited to your applications.
Name:
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Organisation
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Department
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Address
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City
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Postcode
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Tel
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E-mail
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BACKGROUND INFORMATION:
1) Are you currently running SDS-PAGE?
☐Yes
☐No
2) How do you currently stain your proteins? Eg Coomassie, Silver stain? -------------------------------------------------------------3) Who is your current supplier of stain eg PageBlue from Pierce? ----------------------------------------------------------------------4) What are the main drawbacks of your current method? --------------------------------------------------------------------------------5) How many SDS-PAGE do you perform in your lab?
☐ 1 gel/week
☐ 2 - 5 gels/week
☐ 6-10 gels/week
☐ 11-15 gels/week
☐ 15 -20 gels/week
☐ >20 gels/week
EXPERIMENTAL DETAILS:
Triple Red
Tel: 01844 201142 Fax: 01844 203650 E-mail: info@triplered.com Web: www.triplered.com
Sample:
1) Typical samples
☐Soluble Proteins
☐Membrane Proteins
☐Recombinant Protein
☐Antibody
☐Virus
☐Other ---------------------------------------------------------------------------------------------------
2) What is the composition of the buffer containing your sample? ----------------------------------------------------------------------Application:
1) Why do you perform SDS-PAGE?
☐ Soluble Expression
☐ Lysates
☐ Western Blot
☐ Protein Purity
☐ FPLC fractions
☐ Protein Yield
☐ Sizing Proteins
☐ Other, please specify
2) What % of SDS-PAGE is performed for expression as opposed to purification ? --------------------------------------------------APPRAISAL:
3) Would you replace your current method with Real time stain in entirety and, if not, what would stop you?
-------------------------------------------------------------------------------------------------------------------------------------------------------------4) How would you rate the importance of the following performance parameters from a scale of 1 to 5? (1 =
unimportant, 5 = critical)
Speed
Price
No additional steps
Ease of Use
Sensitivity to 50-100 ng protein
☐1
☐1
☐1
☐1
☐1
☐2
☐2
☐2
☐2
☐2
☐3
☐3
☐3
☐3
☐3
☐4
☐4
☐4
☐4
☐4
☐5
☐5
☐5
☐5
☐5
Please let us know if you are interested in any of the following products:
☐ Safety Cabinet
☐ Centrifuge
☐CO2 Incubator
☐ Pure Water System
☐ Autoclave
☐ ULT Freezer
Thank you for taking the time to fill in this questionnaire. Please return all sample requests to info@triplered.com.
Triple Red
Tel: 01844 201142 Fax: 01844 203650 E-mail: info@triplered.com Web: www.triplered.com