Supporting figure/table legends
Figure S1. ABA content, ethylene release, fruit firmness, anthocyanin content, soluble sugar,
titratable acid content, and related gene expression in strawberry pulp during development.
Maturity stages: SG, small green stage; LG, large green stage; BG, bright green stage; W,
white stage; T, turning stage; PR, pink ripening stage; R, red ripening stage. Actin mRNA was
used as the internal control. Error bars represent the SE (n= 3).
Figure S2. Effect of deachening, auxin, and ABA treatments on strawberry fruit
development. The achenes were carefully removed from large green stage and then the nonachenes fruits were treated with a lanolin paste containing either the auxin NAA or ABA. The
fruits were sampled at 5 days and 8 days after removed achenes.
Actin mRNA was used as the internal control. Error bars represent the SE (n= 3).
A, Achenes from large green fruit were removed. B, 5 days fruit after deachening. C, 5 days
after deachening fruit + NAA (a lanolin paste containing 1 mM 1-naphthaleneacetic acid in
1% [v/v] DMSO). D, 5 days after deachening fruit + ABA (a lanolin paste containing 0.1 mM
ABA). E, ABA contents. F, IAA contents. G, expression of FaNCED in different treatment
fruits. H, expression of FaCYP707A in different treatment fruits.
Figure S3. Expression of BGs, FaNCEDs, and CYP707A1, and β-glucosidase activity and
ABA content in dehydrated strawberry fruits. Fruits were harvested at pink stage and stored
at 20oC under high RH (95%, control) and low RH (45%, dehydrated fruits). Actin mRNA
was used as the internal control. Error bars represent the SE (n= 3).
Figure S4. Expression of BGs, FaNCEDs, and CYP707A1, and β-glucosidase activity and
ABA content in dehydrated strawberry leaves. Set control as 1 for expression of genes (A).
ABA content and expression level of genes were under lost 30% water in leaves. Error bars
represent the SE (n=3). Stomatal density was observed by microscope of (40×10) times.
Figure S5. Changes in ABA content, and β-glucosidase activity, and expression of
FaNCED2, FaCYP707A1, and FaBGs in strawberry fruits after treatment with
exogenous ABA. Fruits were harvested at white stage, then soaked by 200 μM of
ABA for 15 min. Actin mRNA was used as the internal control. Error bars represent
the SE (n= 3).
Figure S6. Effect of different concentrations of B. cinerea fungal spores on
strawberry fruits. Spore concentration of B. Cinerea fungal was 0 (CK), 104/μl,
105/μl, 106/μl and 107/μl, respectively.
Figure S7. Changes in ABA content and expression of FaNCED2, FaCYP707A1, and FaBG3
during seed germination of strawberry. Actin mRNA was used as the internal control. Error
bars represent the SE (n = 3).
Figure S8. Amino acid sequence alignment of strawberry β-glucosidases with other
β-glucosidase homologs. The deduced amino acid sequences of the eight
strawberry β-glucosidases were aligned with other β-glucosidases homologs.
Identical amino acid residues are highlighted. Sequences corresponding to the
catalytic acid/base and nucleophile consensus sequences are marked by a thick line
over the top of the sequences while those conserved sequences are marked by a thick
line over the top of the sequences while those conserved residues making contacts
with the sugar are marked by : ◊. The alignment was generated using the Clustal X
and MEGA, and shaded with the BOXSHADE 3.21 program.
Figure S9. Chromatogram of the standard substances of five neutral phenolic
compounds.
Figure S10. Chromatogram of standard substance of chlorogenic acid.
Table S1. Real-time PCR primers used in this study.
Table S2. Similarity of Fa/FvBGs based on deduced amino acids.
Table S3. Similarity of Fa/FvBGs and other species BGs based on deduced amino
acids.
Method S1: Exogenous ABA application of detached strawberry fruits
Method S2: Effect of dehydration stress on strawberry fruits
Method S3： Effect of dehydration stress on strawberry leaves
Method S4：Determination of the total β-glucosidase activity
Appendix 1: Supporting Results