Table S1. Summary table of N. ceranae and N. apis prevalence from

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Table S1. Summary table of N. ceranae and N. apis prevalence from studies conducted across several years and countries. Prevalence varies
considerably across space and time, though absolute estimates probably depend to some extent on methods of detection employed (efficiency of
DNA/RNA extraction, PCR primers employed), sample size (number of workers per colony analysed) and when they were collected during the
flight season.
Country
Spain
Spain
Spain
Spain
Spain Montane
Spain supra-mediterranean
Spain Colline
Spain Meso-mediterranean
Spain Termo-mediterranean
Spain Andalusia
Spain Andalusia
Spain Catalonia
Spain Catalonia
Spain Valencia
Spain Valencia
Spain Andalusia
Spain
Spain (Canary Islands)
Spain (Canary Islands)
Spain (Canary Islands)
Spain (Canary Islands)
Spain
Spain
N. ceranae
prevalence (%)
46
43
47
44
35
38
42
56
57
29
65
7
22
27
47
95
67
0
36
32
49
50
70
N. apis
prevalence (%)
15
10
8
11
16
17
3
10
6
10
8
7
0
24
12
19
21
0
0
0
0
14
13
Source
[1]
[1]
[1]
[1]
[1]
[1]
[1]
[1]
[1]
[2]
[2]
[2]
[2]
[2]
[2]
[3]
[3]
[4]
[4]
[4]
[4]
[5]
[5]
No colonies
screened
629
454
526
329
301
454
191
492
256
80
80
30
9
34
17
446
604
18
44
44
45
113
115
Year
2006spring
2006autumn
2007spring
2007autumn
2006/07
2006/07
2006/07
2006/07
2006/07
2002
2007
2002
2007
2002
2007
2006/07
2007
1998
2008
2010
2011
2006
2010
Scotland
78
73
Germany
7
20
Germany
10
24
Germany
19
12
Germany
19
27
Germany
16
22
Germany
5
8
Germany
2
8
Germany
2
5
Germany
2
3
Sweden
3
33
Lithuania
64
68
a
Balkans
84
<1a
Florida/California
60
20
a
Average across Serbia, Bosnia and Herzegovina, Montenegro and FYROM
[6]
[7]
[7]
[7]
[7]
[7]
[7]
[7]
[7]
[7]
[8]
[9]
[10]
[11]
71
220
238
228
209
210
237
226
219
210
967
129
325
76
2011
2005spring
2006spring
2007spring
2008spring
2009spring
2005 autumn
2006autumn
2007autumn
2008autumn
2007
2011
2002/09
2007
References
1.
Martín-Hernández R, Botías C, Bailón EG, Martínez-Salvador A, Prieto L, Meana A, Higes M. 2012 Microsporidia infecting Apis
mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis? Environ. Microbiol. 14, 2127–38. (doi:10.1111/j.14622920.2011.02645.x)
2.
Botías C, Martín-Hernández R, Garrido-Bailón E, González-Porto A, Martínez-Salvador A, De la Rúa P, Meana A, Higes M. 2012 The
growing prevalence of Nosema ceranae in honey bees in Spain, an emerging problem for the last decade. Res. Vet. Sci. 93, 150–5.
(doi:10.1016/j.rvsc.2011.08.002)
3.
Fernández JM, Puerta F, Cousinou M, Dios-Palomares R, Campano F, Redondo L. 2012 Asymptomatic presence of Nosema spp. in
Spanish commercial apiaries. J. Invertebr. Pathol. 111, 106–10. (doi:10.1016/j.jip.2012.06.008)
4.
Muñoz I, Cepero A, Pinto MA, Martín-Hernández R, Higes M, De la Rúa P. 2014 Presence of Nosema ceranae associated with honeybee
queen introductions. Infect. Genet. Evol. 23, 161–8. (doi:10.1016/j.meegid.2014.02.008)
5.
Jara L, Cepero A, Garrido-Bailón E, Martín-Hernández R, Higes M, De la Rúa P. 2012 Linking evolutionary lineage with parasite and
pathogen prevalence in the Iberian honey bee. J. Invertebr. Pathol. 110, 8–13. (doi:10.1016/j.jip.2012.01.007)
6.
Bollan KA, Hothersall JD, Moffat C, Durkacz J, Saranzewa N, Wright GA, Raine NE, Highet F, Connolly CN. 2013 The microsporidian
parasites Nosema ceranae and Nosema apis are widespread in honeybee (Apis mellifera) colonies across Scotland. Parasitol. Res. 112,
751–9. (doi:10.1007/s00436-012-3195-0)
7.
Gisder S, Hedtke K, Möckel N, Frielitz MC, Linde A, Genersch E. 2010 Five-year cohort study of Nosema spp. in Germany: does climate
shape virulence and assertiveness of Nosema ceranae? Appl. Environ. Microbiol. 76, 3032–3038. (doi:10.1128/aem.03097-09)
8.
Forsgren E, Fries I. 2013 Temporal study of Nosema spp. in a cold climate. Environ. Microbiol. Rep. 5, 78–82. (doi:10.1111/j.17582229.2012.00386.x)
9.
Blažytė-Čereškienė L, Skrodenytė-Arbačiauskienė V, Būda V. 2014 Microsporidian parasites of honey bees Nosema ceranae and N. apis
in Lithuania: supplementary data on occurrence along Europe. J. Apic. Res. 53, 374–376. (doi:10.3896/IBRA.1.53.3.04)
10.
Stevanovic J, Stanimirovic Z, Genersch E, Kovacevic SR, Ljubenkovic J, Radakovic M, Aleksic N. 2011 Dominance of Nosema ceranae
in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. Apidologie 42, 49–58.
(doi:10.1051/apido/2010034)
11.
Vanengelsdorp D, et al. 2009 Colony collapse disorder: a descriptive study. PLoS One 4, e6481. (doi:10.1371/journal.pone.0006481)
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