Variability in microbial community composition and function between different niches within a coral reef
Jessica Tout1*, Thomas C. Jeffries 1, Nicole S. Webster2, Roman Stocker 3, Peter J. Ralph1, Justin R. Seymour1
1
Plant Functional Biology & Climate Change Cluster, University of Technology, Sydney, NSW, Australia.
2
Australian Institute of Marine Science, Townsville, Qld, Australia.
3
Ralph M. Parsons Laboratory, Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge MA
USA.
*
Jessica.tout@student.uts.edu.au
+61 4 31 499 465
Online Resource Table 1 PRIMER table showing SIMPER analysis of the top seven Genera between coral (lagoon-coral and reef crest-coral) and non-coral (open water and sandy substrate)
metagenomes with an average dissimilarity of 9.73. Oligotrophic genera such as Synechococcus and Prochlorococcus were discriminating genera of the non-coral metagenomes as they had a
higher average abundance compared to the coral metagenomes and overall contributed to a cumulative 5.7% of dissimilarity between the metagenomes. Copiotrophic genera such as
Alteromonas, Mycobacterium and Vibrio were shown to be discriminating genera in the coral metagenomes as they had a higher average abundance compared to the non-coral metagenome and
contributed up to 3.6% of the dissimilarity between metagenomes.
Groups
Coral (reef crest &
lagoon)
Non coral (sandy
substrate & open
water)
Average abundance
Average abundance
Average
dissimilarity
Dissimilarity/SD
Contribution
%
Cumulative %
Synechococcus
1.87
2.76
0.32
1.34
3.33
3.33
Prochlorococcus
1.49
2.12
0.23
1.42
2.32
5.65
Alteromonas
0.88
0.46
0.15
0.95
1.58
7.23
T4-like phages
1.65
1.34
0.11
2.38
1.17
8.40
Ciona (sea squirt)
0.00
0.30
0.11
125.97
1.10
9.50
Mycobacterium
0.75
0.53
0.10
1.41
1.02
10.52
Vibrio
1.51
1.24
0.10
1.27
0.99
11.52
Genera