2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > TOOLS > MY GRADES > VIEW ATTEMPTS > REVIEW ASSESSMENT: UNIT 01
STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/4/09 6:23 PM
Name
Unit 01 Study questions
Status
Completed
Score
6 out of 15 points
Instructions
Question 1
0 out of 1 points
Messenger RNA is different from other types of RNA because messenger RNA has:
Selected Answer:
Question 2
1 out of 1 points
Which of the following is the correct order of the steps that occur in a standard PCR cycle?
Selected Answer:
Question 3
Promoter +, Operator +, Repressor -, no inducer present
0 out of 1 points
Patient DNA has been mixed in separate wells with a probe that detects the G1691A mutation
associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is
added to all wells, and the fluorescence is measured in each well. Fluorescence was detected
in the well that included the normal probe, and no fluorescence was detected in the well
containing the mutant probe. Which of the following is the correct interpretation of these
results?
Selected
Answer:
Question 7
30 μg/mL
1 out of 1 points
In the lactose operon, which of the following configurations would result in gene expression?
Selected Answer:
Question 6
Multiplex inversion probe assay
0 out of 1 points
When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance
reading is multiplied by the dilution and the absorptivity constant, which is:
Selected Answer:
Question 5
Denaturation, annealing, and extension
0 out of 1 points
Which of the following methods can be used to screen for the presence of unknown mutations?
Selected Answer:
Question 4
introns and exons
The results are not conclusive either way and should be confirmed
using a different method.
1 out of 1 points
The process of separating the two DNA strands into two single strands is called:
Selected Answer:
Question 8
0 out of 1 points
The codons that do not code for an amino acid and terminate protein synthesis are what kind
of codon?
Selected Answer:
Question 9
denaturation
Anticodon
1 out of 1 points
SyBr green fluorescence is detectable at which stage of the PCR amplification process?
Selected Answer:
Question 10
0 out of 1 points
Which of the following types of RNA is directly involved in removing introns from RNA in
eukaryotes?
Selected Answer:
Question 11
Primer extension
Transfer
1 out of 1 points
The loop of transfer RNA that interacts with the codon on mRNA in translation is called the:
Selected Answer:
anticodon loop
Question 12
0 out of 1 points
The melting temperature of nucleic acid is the temperature when:
Selected Answer:
Question 13
all of the double-stranded DNA is single-stranded
0 out of 1 points
The term used to describe the arrangement of the individual strands in the double-stranded
DNA molecule is:
Selected Answer:
parallel
Question 14
1 out of 1 points
A protein that has lost its tertiary structure is called:
Selected Answer:
Question 15
denatured
0 out of 1 points
A chromosome that has the centromere in the center of the chromosome is called:
Selected Answer:
telocentric
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 1:50 PM
Name
Unit 01 Study questions
Status
Completed
Score
8 out of 15 points
Instructions
Question 1
1 out of 1 points
A reference segment of DNA has the following sequence: GCTCACCATGG. The fourth
nucleotide is changed to an A in a particular genetic disease. How would this mutation be
denoted?
Selected Answer:
Question 2
4C>A
0 out of 1 points
A technologist performs fluorescence in situ hybridization (FISH) on interphase chromosomes
using a probe to chromosome 21. In all cells examined, two signals were seen. These results
are interpreted as:
Selected Answer:
Abnormal; the patient has a Philadelphia chromosome
Question 3
0 out of 1 points
The set of nucleotides in mRNA that encode for an amino acid is called a(n):
Selected Answer:
Question 4
1 out of 1 points
Which of the following PCR modifications was developed to allow for the amplification of an
RNA template?
Selected Answer:
Question 5
codon
Reverse transcriptase
0 out of 1 points
Human chromosomes are described as which of the following with regard to the location of the
centromere?
Selected Answer:
Genocentric
Question 6
0 out of 1 points
Which of the following conditions are the least stringent?
Selected Answer:
Question 7
40% formamide, 0.2X SSC, 60oC
1 out of 1 points
When gene expression is regulated by mechanisms other than the interaction of cis elements
and trans factors, the regulation is called:
Selected Answer:
epigenetics
Question 8
1 out of 1 points
When chromosomes are stained with Giemsa, the resulting bands are called:
Selected Answer:
Question 9
G bands
1 out of 1 points
A band that is present in both the hydrazine and hydrazine plus salt-treated sample lanes is
read as which of the following nucleotides?
Selected Answer:
T
Question 10
0 out of 1 points
Which DNA sequence is homologous to 5′GATTCTCAAAGGACT3′?
Selected Answer:
Question 11
1 out of 1 points
The fundamental physical and functional unit of inheritance that encodes a functional product
is a:
Selected Answer:
Question 12
Pulse–field gel electrophoresis
1 out of 1 points
Which of the following types of chromatin are open and actively involved in gene expression
(transcription)?
Selected Answer:
Question 15
β′
0 out of 1 points
A very limited amount of nucleic acid, 500-1500 bp in size, needs to be analyzed in a short
time (same day) with the results available immediately. Which of the following electrophoresis
procedures will satisfy those conditions?
Selected Answer:
Question 14
gene
0 out of 1 points
Which component of RNA polymerase is responsible for initiating transcription at the right
site?
Selected Answer:
Question 13
5′GATTCTCAAAGGACT3′
Euchromatin
1 out of 1 points
The entire human genome can be analyzed at one time by which of the following methods?
Selected Answer:
Microarray
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 3:08 PM
Name
Unit 01 Study questions
Status
Completed
Score
7 out of 15 points
Instructions
Question 1
1 out of 1 points
Which of the following is the first step in DNA isolation from cells in a clinical sample?
Selected Answer:
Question 2
0 out of 1 points
A portion of a sequencing gel is described as follows: the first band at the bottom of the gel
was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP
lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the
ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as
determined on this gel?
Selected Answer:
Question 3
4C>A
1 out of 1 points
All but two amino acids are encoded by more than one codon, thus making the genetic code:
Selected Answer:
Question 6
Capillary electrophoresis
1 out of 1 points
A reference segment of DNA has the following sequence: GCTCACCATGG. The fourth
nucleotide is changed to an A in a particular genetic disease. How would this mutation be
denoted?
Selected Answer:
Question 5
5′GACCTAGCTA3′
0 out of 1 points
A molecular biologist needs to separate and analyze a mixture of nucleic acids that are about
100,000 bp in size. Which of the following methods will yield the best results?
Selected Answer:
Question 4
Lysis of cells
redundant
0 out of 1 points
A band that is present in only the formic acid–treated sample lanes is read as which of the
following nucleotides?
Selected Answer:
G
Question 7
0 out of 1 points
DNA replication requires the presence of:
Selected Answer:
RNA polymerase
Question 8
0 out of 1 points
RNA is degraded by:
Selected Answer:
Question 9
polymerases
1 out of 1 points
In order to monitor the progress of electrophoresis, which of the following is added to a sample
that does not associate with DNA and runs ahead of the smallest fragments in the sample?
Selected Answer:
Question 10
0 out of 1 points
What is the yield of RNA from the sample in which 1:10 dilution had an absorbance reading of
0.675 at 260nm when the sample has a volume of 0.4 mL?
Selected Answer:
Question 11
a purine and a pyrimidine
1 out of 1 points
When gene expression is regulated by mechanisms other than the interaction of cis elements
and trans factors, the regulation is called:
Selected Answer:
Question 15
β
1 out of 1 points
In a double-stranded DNA molecule, base-pairing between strands occurs between:
Selected Answer:
Question 14
depolymerization
0 out of 1 points
Which component of RNA polymerase is responsible for initiating transcription at the right
site?
Selected Answer:
Question 13
13.5 μg
0 out of 1 points
The process of separating the two DNA strands into two single strands is called:
Selected Answer:
Question 12
Bromophenol blue
epigenetics
1 out of 1 points
When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance
reading is multiplied by the dilution and the absorptivity constant, which is:
Selected Answer:
40 μg/mL
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 3:28 PM
Name
Unit 01 Study questions
Status
Completed
Score
6 out of 15 points
Instructions
Question 1
1 out of 1 points
Which of the following is the first step in DNA isolation from cells in a clinical sample?
Selected Answer:
Question 2
Lysis of cells
1 out of 1 points
Which of the following is the correct order of steps when performing a Southern blot after
isolation of the DNA?
Selected
Answer:
Restriction enzyme digestion, electrophoresis, denaturation, probe
hybridization
Question 3
0 out of 1 points
One DNA strand binds to the other strand through:
Selected Answer:
phosphodiester bonds
Question 4
1 out of 1 points
The large ribosome subunit in prokaryotes consists of ribosomal proteins and:
Selected Answer:
Question 5
0 out of 1 points
Colcemid is used in the procedure of preparing a chromosome spread for karyotype analysis to
do which of the following?
Selected Answer:
Question 6
Fix cells to the slide prior to staining
1 out of 1 points
A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the
fragments were run on the gel, there were bands distributed through the lanes corresponding
to ddTTP, ddCTP, and ddATP. In the ddGTP lane, there was one large band at the bottom of
the gel. What is the interpretation of these results?
Selected Answer:
Question 7
23S rRNA and 5S rRNA
No dGTP was added to the reaction mix.
0 out of 1 points
A DNA change that affects the structure of entire chromosomes is which type of mutation?
Selected Answer:
Question 8
0 out of 1 points
In inorganic DNA isolation or “salting out” procedures, in the presence of low pH and high salt
concentrations, which intracellular component precipitates out of solution?
Selected Answer:
Question 9
Nucleotide
DNA
1 out of 1 points
A portion of a sequencing gel is described as follows: the first band at the bottom of the gel
was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP
lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the
ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as
determined on this gel?
Selected Answer:
Question 10
0 out of 1 points
Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191.
What is the 260 nm/280 nm ratio?
Selected Answer:
Question 11
conjugation
1 out of 1 points
Which of the following materials is most commonly used to bind nucleic acids in blotting
procedures?
Selected Answer:
Question 13
1.6
0 out of 1 points
The movement of DNA from one bacterium to another through the activity of bacteriophages
is called:
Selected Answer:
Question 12
5′ATCGATCCAG3′
Nitrocellulose
0 out of 1 points
A melt curve analysis was performed to genotype hepatitis C virus (HCV), which has been
identified in a patient. When the derivative of fluorescence was plotted versus the temperature
for the patient's isolate as well as for a known genotype of HCV, the two sequences had
identical peaks and thus the same Tms. Which of the following is the correct interpretation of
these results?
Selected Answer:
The patient's HCV genotype is different from the known genotype.
Question 14
0 out of 1 points
Transcription-mediated amplification obtains:
Selected Answer:
Question 15
DNA from RNA and RNA from DNA
0 out of 1 points
In the Sanger sequencing method, which of the following when incorporated into the growing
strand are used to determine the identity of the base at a particular position in a piece of
DNA?
Selected Answer:
Ribonucleotides
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 3:28 PM
Name
Unit 01 Study questions
Status
Completed
Score
6 out of 15 points
Instructions
Question 1
1 out of 1 points
Which of the following is the first step in DNA isolation from cells in a clinical sample?
Selected Answer:
Question 2
Lysis of cells
1 out of 1 points
Which of the following is the correct order of steps when performing a Southern blot after
isolation of the DNA?
Selected
Answer:
Restriction enzyme digestion, electrophoresis, denaturation, probe
hybridization
Question 3
0 out of 1 points
One DNA strand binds to the other strand through:
Selected Answer:
phosphodiester bonds
Question 4
1 out of 1 points
The large ribosome subunit in prokaryotes consists of ribosomal proteins and:
Selected Answer:
Question 5
0 out of 1 points
Colcemid is used in the procedure of preparing a chromosome spread for karyotype analysis to
do which of the following?
Selected Answer:
Question 6
23S rRNA and 5S rRNA
Fix cells to the slide prior to staining
1 out of 1 points
A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the
fragments were run on the gel, there were bands distributed through the lanes corresponding
to ddTTP, ddCTP, and ddATP. In the ddGTP lane, there was one large band at the bottom of
the gel. What is the interpretation of these results?
Selected Answer:
Question 7
0 out of 1 points
A DNA change that affects the structure of entire chromosomes is which type of mutation?
Selected Answer:
Question 8
Nucleotide
0 out of 1 points
In inorganic DNA isolation or “salting out” procedures, in the presence of low pH and high salt
concentrations, which intracellular component precipitates out of solution?
Selected Answer:
Question 9
No dGTP was added to the reaction mix.
DNA
1 out of 1 points
A portion of a sequencing gel is described as follows: the first band at the bottom of the gel
was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP
lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the
ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as
determined on this gel?
Selected Answer:
Question 10
0 out of 1 points
Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191.
What is the 260 nm/280 nm ratio?
Selected Answer:
Question 11
conjugation
1 out of 1 points
Which of the following materials is most commonly used to bind nucleic acids in blotting
procedures?
Selected Answer:
Question 13
1.6
0 out of 1 points
The movement of DNA from one bacterium to another through the activity of bacteriophages
is called:
Selected Answer:
Question 12
5′ATCGATCCAG3′
Nitrocellulose
0 out of 1 points
A melt curve analysis was performed to genotype hepatitis C virus (HCV), which has been
identified in a patient. When the derivative of fluorescence was plotted versus the temperature
for the patient's isolate as well as for a known genotype of HCV, the two sequences had
identical peaks and thus the same Tms. Which of the following is the correct interpretation of
these results?
Selected Answer:
The patient's HCV genotype is different from the known genotype.
Question 14
0 out of 1 points
Transcription-mediated amplification obtains:
Selected Answer:
DNA from RNA and RNA from DNA
Question 15
0 out of 1 points
In the Sanger sequencing method, which of the following when incorporated into the growing
strand are used to determine the identity of the base at a particular position in a piece of
DNA?
Selected Answer:
Ribonucleotides
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 3:45 PM
Name
Unit 01 Study questions
Status
Completed
Score
9 out of 15 points
Instructions
Question 1
1 out of 1 points
In the lactose operon, which of the following configurations would result in gene expression?
Selected Answer:
Question 2
Promoter +, Operator +, Repressor -, no inducer present
0 out of 1 points
The 260 nm/280 nm ratio for isolated DNA was determined to be 1.2. What should be done
next with this sample?
Selected Answer:
Dilute the sample 1:10 and repeat spectrophotometry.
Question 3
1 out of 1 points
Which DNA sequence is homologous to 5′GATTCTCAAAGGACT3′?
Selected Answer:
Question 4
3′CTAAGAGTTTCCTGA5′
1 out of 1 points
When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance
reading is multiplied by the dilution and the absorptivity constant, which is:
Selected Answer:
40 μg/mL
Question 5
0 out of 1 points
An exon is:
Selected Answer:
Question 6
within 100 bp of ORFs
0 out of 1 points
Which of the following assays can be used to detect multiple mutations simultaneously?
Selected Answer:
Question 7
Heteroduplex analysis
1 out of 1 points
DNA quality can be assessed relatively simply and quickly by which of the following
procedures?
Selected Answer:
Electrophoresis
Question 8
1 out of 1 points
Which end of the sequenced DNA is found at the bottom of a sequencing gel?
5′
Selected Answer:
Question 9
0 out of 1 points
Nucleic acid has been isolated from a urethral swab and a PCR is performed on the sample in
order to detect Neisseria gonorrhoeae. The positive control has a band at the expected size;
the negative template control and the contamination controls do not have bands; and the
amplification control has a band of expected size. The patient sample has a band consistent
with the amplification control band and does not have a band corresponding to the positive
control band. How is this result interpreted?
Selected Answer:
Question 10
The patient has a false negative result for Neisseria gonorrhoeae.
1 out of 1 points
Which of the following techniques is a primer-directed in vitro enzymatic reaction for the
production of multiple copies of a specific DNA fragment found in a clinical sample?
Selected Answer:
Question 11
Polymerase chain reaction
1 out of 1 points
Which of the following is the correct order of steps when performing a Southern blot after
isolation of the DNA?
Selected
Answer:
Restriction enzyme digestion, electrophoresis, denaturation, probe
hybridization
Question 12
0 out of 1 points
Which of the following solutions has the highest stringency?
Selected Answer:
Question 13
1 out of 1 points
What is the next step in the Southern blot procedure after restriction enzyme digestion?
Selected Answer:
Question 14
Gel electrophoresis
0 out of 1 points
What is the melting temperature (Tm) of the following hybrid: AGCTATGCCGGCTAGCAC
TCGATACGGCCGATCGTG
Selected Answer:58
Question 15
High salt buffer
65oC
1 out of 1 points
As compared with traditional slab gel electrophoresis, which of the following is not an
advantage of capillary electrophoresis?
Selected Answer:
Cost of instrumentation and labels
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 6:13 PM
Name
Unit 01 Study questions
Status
Completed
Score
12 out of 15 points
Instructions
Question 1
1 out of 1 points
A molecular biologist needs to separate a mixture of oligonucleotides with a resolution between
bands of 1 bp. Which of the following methods should be used?
Selected Answer:
Question 2
Polyacrylamide gel electrophoresis
1 out of 1 points
Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191.
What is the 260 nm/280 nm ratio?
Selected Answer:
1.4
Question 3
1 out of 1 points
The short arm of the chromosome is designated as which of the following?
Selected Answer:
p
Question 4
0 out of 1 points
Real-time PCR differs from standard PCR in which way?
Selected Answer:
Question 5
1 out of 1 points
Which of the following molecules polymerize to form a support medium through which nucleic
acids move?
Selected Answer:
Question 6
Standard PCR is faster.
Polyacrylamide
0 out of 1 points
DNA sequences that are involved in the regulation of gene expression are called:
Selected Answer:
Question 7
repressors
1 out of 1 points
Which of the following bind(s) specifically to a sequence of interest, thus facilitating the
analysis of that sequence?
Selected Answer:
Probes
Question 8
1 out of 1 points
Which end of the sequenced DNA is found at the bottom of a sequencing gel?
Selected Answer:
Question 9
5′
1 out of 1 points
The sequence of amino acids in a protein constitutes which level of protein structure?
Selected Answer:
Question 10
Primary
1 out of 1 points
A melt curve analysis was performed to genotype hepatitis C virus (HCV), which has been
identified in a patient. When the derivative of fluorescence was plotted versus the temperature
for the patient's isolate as well as for a known genotype of HCV, the two sequences had
identical peaks and thus the same Tms. Which of the following is the correct interpretation of
these results?
Selected Answer:
The patient's HCV genotype is the same as the known genotype.
Question 11
1 out of 1 points
DNA replication requires the presence of:
Selected Answer:
DNA template
Question 12
0 out of 1 points
An exon is:
Selected Answer:
Question 13
within 100 bp of ORFs
1 out of 1 points
DNA quality can be assessed relatively simply and quickly by which of the following
procedures?
Selected Answer:
Electrophoresis
Question 14
1 out of 1 points
One DNA strand binds to the other strand through:
Selected Answer:
Question 15
hydrogen bonds
1 out of 1 points
The fundamental physical and functional unit of inheritance that encodes a functional product
is a:
Selected Answer:
gene
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 6:24 PM
Name
Unit 01 Study questions
Status
Completed
Score
10 out of 15 points
Instructions
Question 1
1 out of 1 points
The loop of transfer RNA that interacts with the codon on mRNA in translation is called the:
Selected Answer:
anticodon loop
Question 2
0 out of 1 points
Genomic imprinting is accomplished primarily through:
Selected Answer:
transcription
Question 3
0 out of 1 points
The genetic code consists of a sequence of how many nucleotides?
Selected Answer:
Question 4
Four
1 out of 1 points
Which of the following laboratory methods used to detect mutations relies on differences in
melt characteristics between two DNA samples that have different sequences such that
double-stranded DNA becomes single-stranded at different points in a gel?
Selected Answer:
Denaturing gradient gel electrophoresis
Question 5
1 out of 1 points
One DNA strand binds to the other strand through:
Selected Answer:
hydrogen bonds
Question 6
0 out of 1 points
The set of nucleotides in mRNA that encode for an amino acid is called a(n):
Selected Answer:
anticodon
Question 7
1 out of 1 points
The short arm of the chromosome is designated as which of the following?
Selected Answer:
p
Question 8
1 out of 1 points
In PCR, an instrument is used to increase and decrease the temperature at set intervals as
programmed by the technologist in order to:
Selected Answer:
Question 9
determine the reaction that is occurring in the sample
1 out of 1 points
In inorganic DNA isolation or “salting out” procedures, in the presence of low pH and high salt
concentrations, which intracellular component precipitates out of solution?
Selected Answer:
Protein
Question 10
0 out of 1 points
Which of the following is a purine?
Selected Answer:
Cytosine
Question 11
1 out of 1 points
The most abundant form of RNA in all cells is:
Selected Answer:
ribosomal
Question 12
1 out of 1 points
Which of the following is a cis factor of the lactose operon?
Selected Answer:
Question 13
1 out of 1 points
PolyT oligomers bound to a matrix resin column will selectively isolate which of the following?
Selected Answer:
Question 14
Operator
Messenger RNA
0 out of 1 points
Human chromosomes are described as which of the following with regard to the location of
the centromere?
Selected Answer:
Holocentric
Question 15
1 out of 1 points
The enzyme that bonds two amino acids together in protein synthesis is:
Selected Answer:
peptidyl transferase
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/5/09 8:38 PM
Name
Unit 01 Study questions
Status
Completed
Score
11 out of 15 points
Instructions
Question 1
0 out of 1 points
Patient DNA has been mixed in separate wells with a probe that detects the G1691A mutation
associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is
added to all wells, and the fluorescence is measured in each well. Fluorescence was detected
in the well that included the normal probe, and no fluorescence was detected in the well
containing the mutant probe. Which of the following assays was performed?
Selected Answer:
Question 2
1 out of 1 points
Taqman, molecular beacons, and scorpion-type primers are all used in which of the following
procedures?
Selected Answer:
Question 3
Quantitative PCR
1 out of 1 points
Human chromosomes are described as which of the following with regard to the location of the
centromere?
Selected Answer:
Question 4
High-density oligonucleotide array
Acrocentric
0 out of 1 points
What is the concentration of RNA whereby a 1:10 dilution has an absorbance reading of 0.675
at 260 nm?
Selected Answer:
33.75 μg/mL
Question 5
1 out of 1 points
Which of the following enzymes performs transcription in bacteria?
Selected Answer:
Question 6
DNA-dependent RNA polymerase
1 out of 1 points
Which of the following materials is most commonly used to bind nucleic acids in blotting
procedures?
Selected Answer:
Nitrocellulose
Question 7
0 out of 1 points
RNA and DNA are structurally similar because they both:
Selected Answer:
Question 8
have ribose as their sugar moiety
1 out of 1 points
As compared with traditional slab gel electrophoresis, which of the following is not an
advantage of capillary electrophoresis?
Selected Answer:
Question 9
Cost of instrumentation and labels
1 out of 1 points
When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance
reading is multiplied by the dilution and the absorptivity constant, which is:
Selected Answer:
Question 10
40 μg/mL
1 out of 1 points
DNA quality can be assessed relatively simply and quickly by which of the following
procedures?
Selected Answer:
Electrophoresis
Question 11
0 out of 1 points
Transcription-mediated amplification obtains:
Selected Answer:
DNA from RNA
Question 12
1 out of 1 points
The most abundant form of RNA in all cells is:
Selected Answer:
ribosomal
Question 13
1 out of 1 points
The matrix in capillary electrophoresis through which nucleic acids pass is:
Selected Answer:
silica
Question 14
1 out of 1 points
The enzyme that bonds two amino acids together in protein synthesis is:
Selected Answer:
Question 15
peptidyl transferase
1 out of 1 points
Which component of RNA polymerase is responsible for initiating transcription at the right
site?
Selected Answer:
σ
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > TOOLS > MY GRADES > VIEW ATTEMPTS > REVIEW ASSESSMENT: UNIT 01
STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/6/09 6:30 PM
Name
Unit 01 Study questions
Status
Completed
Score
12 out of 15 points
Instructions
Question 1
1 out of 1 points
Nucleic acid has been isolated from a urethral swab and a PCR is performed on the sample in
order to detect Neisseria gonorrhoeae. The positive control has a band at the expected size;
the negative template control and the contamination controls do not have bands; and the
amplification control has a band of expected size. The patient sample has a band consistent
with the amplification control band and does not have a band corresponding to the positive
control band. How is this result interpreted?
Selected
Answer:
The patient does not have Neisseria gonorrhoeae, and it is a true
negative result.
Question 2
1 out of 1 points
The large ribosome subunit in prokaryotes consists of ribosomal proteins and:
Selected Answer:
Question 3
1 out of 1 points
A change in DNA sequence that is present in at least 1%-2% of the population is called a(n):
Selected Answer:
Question 4
polymorphism
1 out of 1 points
When gene expression is regulated by mechanisms other than the interaction of cis elements
and trans factors, the regulation is called:
Selected Answer:
Question 5
23S rRNA and 5S rRNA
epigenetics
1 out of 1 points
Which of the following types of chromatin are open and actively involved in gene expression
(transcription)?
Selected Answer:
Euchromatin
Question 6
1 out of 1 points
The process of protein synthesis is also called:
Selected Answer:
translation
Question 7
1 out of 1 points
Which end of the sequenced DNA is found at the bottom of a sequencing gel?
Selected Answer:
Question 8
5′
1 out of 1 points
Which of the following is the binding site for the repressor protein of the lactose operon?
Selected Answer:
Operator
Question 9
1 out of 1 points
The movement of DNA from one bacterium to another through the activity of bacteriophages is
called:
Selected Answer:
Question 10
transduction
1 out of 1 points
DNA quality can be assessed relatively simply and quickly by which of the following
procedures?
Selected Answer:
Question 11
0 out of 1 points
A DNA-specific dye that is used in fluorometry procedures to measure DNA concentration is:
Selected Answer:
Question 12
Phosphodiester
0 out of 1 points
A band that is present in only the formic acid–treated sample lanes is read as which of the
following nucleotides?
Selected Answer:
Question 15
Derivative chromosome
1 out of 1 points
The nucleotides in a single strand of DNA are held together by which of the following bonds?
Selected Answer:
Question 14
SyBr Green
1 out of 1 points
A chromosome formed when parts of two or more chromosomes are joined to a third normal
chromosome is called a(n):
Selected Answer:
Question 13
Electrophoresis
T
0 out of 1 points
In organic isolation of RNA procedures, guanidinium isothiocyanate is added to:
Selected Answer:
separate RNA from DNA
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User
Lisa Lavery
Submitted
10/8/09 7:47 PM
Name
Unit 01 Study questions
Status
Completed
Score
10 out of 15 points
Instructions
Question 1
1 out of 1 points
The codons that do not code for an amino acid and terminate protein synthesis are what kind
of codon?
Selected Answer:
Question 2
1 out of 1 points
In order to increase the density of a sample relative to the density of the buffer prior to loading
the sample into a gel, which of the following is added to the sample?
Selected Answer:
Question 3
metacentric
1 out of 1 points
Which of the following types of chromatin are open and actively involved in gene expression
(transcription)?
Selected Answer:
Question 5
Glycerol
1 out of 1 points
A chromosome that has the centromere in the center of the chromosome is called:
Selected Answer:
Question 4
Nonsense
Euchromatin
0 out of 1 points
A very limited amount of nucleic acid, 500-1500 bp in size, needs to be analyzed in a short
time (same day) with the results available immediately. Which of the following electrophoresis
procedures will satisfy those conditions?
Selected Answer:
Pulse–field gel electrophoresis
Question 6
1 out of 1 points
A protein that has lost its tertiary structure is called:
Selected Answer:
Question 7
1 out of 1 points
Dye terminator sequencing has been performed using capillary electrophoresis to separate the
fragments. ddATP has been labeled with a green fluor, ddTTP with red, ddCTP with blue, and
ddGTP with black. The colors are detected in the following order: red, green, black, red, green,
blue, red, black, black, green, blue. What is the sequence of this fragment?
Selected Answer:
Question 8
denatured
TAGTACTGGAC
0 out of 1 points
A PCR assay is performed, and the results are being analyzed. The positive control has a band
at the expected size; the negative template control has a band at the same size as the positive
control; the contamination control has no band; and the amplification control has a band of
expected size. All of the patient samples that were run had a band the same size as the band
seen in the positive and negative template control wells. How are these results interpreted?
Selected
Answer:
Question 9
This run is not acceptable because the contamination control should
have a band.
1 out of 1 points
The nucleotides in a single strand of DNA are held together by which of the following bonds?
Selected Answer:
Question 10
1 out of 1 points
The loop of transfer RNA that interacts with the codon on mRNA in translation is called the:
Selected Answer:
Question 11
anticodon loop
0 out of 1 points
A molecular biologist needs to separate and analyze a mixture of nucleic acids that are about
100,000 bp in size. Which of the following methods will yield the best results?
Selected Answer:
Question 12
Phosphodiester
Capillary electrophoresis
0 out of 1 points
In which of the following nucleic acid amplification procedures is target nucleic acid labeled
with enough signal to be detectable?
Selected Answer:
Polymerase chain reaction
Question 13
1 out of 1 points
In an RNA molecule, adenine always base-pairs with:
Selected Answer:
uracil
Question 14
1 out of 1 points
The matrix in capillary electrophoresis through which nucleic acids pass is:
Selected Answer:
silica
Question 15
0 out of 1 points
Which of the following solutions has the highest stringency?
Selected Answer:
High salt buffer