KIGALI INSTITUTE OF SCIENCE AND TECHNOLOGY
INSTITUT DES SCIENCES ET TECHNOLOGIE
Avenue de l'Armée, B.P. 3900 Kigali, Rwanda
INSTITUTE EXAMINATIONS – ACADEMIC YEAR 2012-2013
SUPPLEMANTARY EXAMINATION
FACULTY OF SCIENCE
SCIENCE 3-BIOLOGY
FOURTH YEAR SEMESTER II
BIO 3426- Advanced Recombinant DNA Technology
DATE:………./……../2013
TIME: 2 HOURS
MAXIMUM MARKS = 60
INSTRUCTIONS
1. This paper consists of two sections A and B.
2. Answer all questions in section A, and any TWO out of THREE questions in
Section B.
3. No written materials are allowed into the examination room.
4. Write all your answers in the answer booklet provided.
5. Do not forget to write your registration number.
6. Do not write any answers on this question paper.
7. Where appropriate draw large clearly labeled diagrams in your answers.
Section A Compulsory
Question 1
20 marks
a)
How are restriction enzymes such as EcoRI named?
4 marks
b)
How plasmids are named?
3 marks
c)
d)
Give two types of plasmid with their functions.
2 marks
What are the advantages and disadvantages of M13 vector in genetic engineering?
3 marks
e)
What are the differences between the lytic and lysogenic life cycles of typical bacterial
viruses?
4 marks
f)
What are the differences between plasmid, cosmid and YAC? Under what circumstances
are each useful?
4 marks
Section B Answer any two questions from the following three questions
Question 2 Discuss the different types of plasmids replication
40 marks
20 marks
Question 3. You are studying an experimental plant known as Musa acuminata. You would like to create
a genomic library of Musa acuminata so you can find the gene responsible for bitter taste called BT
a) You create the genomic library (starting with an EcoRI digesting of the DNA), and identify one
vector that contains BT gene. You decide to analyze this vector further. You cut the vector with
EcoRI and purify the genomic insert. You then digest the insert with 2 different restriction
enzymes SpaII and HindIII. You obtain the following results:
Draw the map of insert genomic indicating the restriction sites for the all enzymes. Please, include the
distance.
8marks
b) You then decided to sequence by Sanger the small portion find of the BT gene found on your
insert. Give all enzymes and reagents that you will need to perform a sequencing experiment.
6 marks
c) You obtain the following results on the gel:
What is the sequence represented on the gel? Be sure to indicate 5’ and 3’ end
3 marks
d) After sequencing the BT obtained from your genomic library you find a 59 base pair insert in the
middle of it that does not correspond to the mRNA for BT. what is that?
3 marks
Question 4
20 marks
a) What is PCR? And when is it used?
4 marks
b) Discuss on different types of PCR and the processing of its products
16 marks
End of question papers