NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING (NIBGE) JHANG ROAD, P.O. BOX: 577, FAISALABAD, PAKISTAN Ph: +92-41-2651475 to 79; Fax: +92-41-2651472; URL: www.nibge.org ISO 9001:2008 ISO# ___________________ Page 1 of 4 NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING (NIBGE) JHANG ROAD, P.O. BOX: 577, FAISALABAD, PAKISTAN Ph: +92-41-2651475 to 79; Fax: +92-41-2651472; URL: www.nibge.org ISO 9001:2008 ISO# ___________________ Standard Operating Procedures (SOPs) for Using NIBGE Animal House (NAH) Facility_ 1. Rationale and purpose of the proposed use of animals for experimentation must be justified. 2. Approval must be obtained on prescribed “Registration form” from animal house committee before starting any experiment related to use of animal for research. 3. Approval of animal experiment(s) and/or use of NIBGE animal house will be granted by the In-charge of NIBGE animal house facility and at least two committee members after discussing the proposed experiments. 4. Experiment must be designed after discussion with any one of the committee member of animal house committee NIBGE. 5. Each division interested to use NAH facility must be authorized/ depute a person(s) involve in work related to NIBGE animal house facility to look after their animal’s health, feeding, cleanliness as well as responsible for maintenance of facility during and after the experimentations. 6. Only authorized users or their nominated person will be allowed to enter/visit facility. No other person would be allowed to enter the facility without permission / knowledge of animal house facility committee. 7. Authorized person make entry into the register for using/ entering the facility. 8. User of NAH facility will bear the cost of animals, their feeding and maintenance. Also contribute in term of human and financial resources for maintenance and smooth running of NIBGE animal house facility. 9. The Scientist /researcher /PI will be responsible to arrange/allocate necessary resources (animals, animal feed, material etc) for the entire duration of the experiment(s). 10. All users should wear personal protection material (e.g. gloves, mask, lab coat etc) while enter and working inside the NAH facility and must be removed and hands properly washed before leaving the facility. 11. Researcher must advice from incharge /committee members of NIBGE animal house facility for the procurement of animals. The use of animals in research requires healthy animals with known backgrounds. 12. Once animals are brought into NAH facility, they would be kept in quarantine for a few days (as suggested by the NAH committee) to avoid contamination / spread of any contagious diseases(s) in other animals in the facility. Any abnormal animal behavior or condition will be immediately reported to the investigator and facility manager 13. All users of the NIBGE Animal house Facility are required to keep daily record of animals on the data form/animal husbandry form as provided by the incharge NAH facility. Page 2 of 4 NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING (NIBGE) JHANG ROAD, P.O. BOX: 577, FAISALABAD, PAKISTAN Ph: +92-41-2651475 to 79; Fax: +92-41-2651472; URL: www.nibge.org ISO 9001:2008 14. Daily observation of animals for any disease/unwanted symptom is required by the user. 15. In case of abnormal animal behavior or severe disease develops into animal(s), these should be immediately reported and removed from the experiment. 16. Feeding, bedding and cleaning of animals/cages should be done by all users/deputed person as suggested by NAH committee /NAH technician. 17. No animal should be taken in or out without the knowledge/ permission of incharge, NIBGE Animal house Facility. 18. All users new to animal experimentation are required to get basic skills for handling/working with animals. 19. All accidents (bites, sharp cuts, fires) should be reported to in-charge, NAH Facility and documented in a register maintained at NAH facility for this purpose. 20. After the completion of experiment, the user can donate un-used extra animals to NAH Facility. However, users are responsible to remove/exterminate animals used in their experimentation from NAH facility in consultation with the Incharge of the facility. 21. Extermination and disposal of animals would be carried out as suggested by the NIBGE Animal house Facility committee. Page 3 of 4 NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING (NIBGE) JHANG ROAD, P.O. BOX: 577, FAISALABAD, PAKISTAN Ph: +92-41-2651475 to 79; Fax: +92-41-2651472; URL: www.nibge.org ISO 9001:2008 ISO# __________________ REGISTRATION FORM FOR USING NIBGE ANIMAL HOUSE FACILITY For Whom Required: _____________________________________________________________________________________________ Title of Experiment: ______________ ___ Project name under experiment is conducting: ___ Experiment starts date: ___ Experiment end date: ___ Animal type (please encircle): Mice Rats Rabbits Other (provide name) Number of animals to be used: ___ Name of animal house committee member to whom discussed/designed your experiments: __________________________________________________________________________________ Undertaking: 1. 2. By submitting this form for experiments on animal, I/We agree to follow the SOP for use of experimental animals as laid down by the NIBGE animal house committee. I/We will contribute human and financial resources (for animals maintenance/ feeding etc) to maintain NIBGE animal house/facility for the duration of my/our experiments as requested by the NIBGE animal house committee. Name and Signature of research worker (scientist/researcher/student) responsible for animal work: _________________________________Signature with dates: _________________________ Name & Designation of a person deputed to look after the animals: _________________________ Name and Signature of Group Leader/Supervisor /PI:_____________________________________ Signature with Date:______________________________ Head of Division: __________________________ Approved by NIBGE Animal House Committee members: 1……………………………………, 2…………..…………………., In-charge NIBGE Animal House: ____________________ 3……………………………………… Date: ___________________ ____________________ Director, NIBGE Page 4 of 4