Supplemental Table S1. Sorghum genes annotated under the major anthracnose resistance QTL on sorghum chromosome 5. Genes shown are those that lie underneath the QTL interval where the LOD
score exceeded the 3.9 LOD significance threshold. Amino acid changes in genes associated with plant disease resistance were detected using resequencing data of parent SC748-5 in comparison to parent
BTx623. Arabidopsis orthologs were found using the Rice Genome Annotation Project (www. http://rice.plantbiology.msu.edu).
Gene
AA
Changes
Start
Position
Stop Position
Biological Process
Sb05g022160
3
53814166
53815489
Sb05g022230
4
Arabidopsis
Ortholog
Annotated Function
Citation in Literature
AT5G43590
patatin putative
(Yang et al., 2007)
53883773
53888305
defense response to fungus
salicylic acid biosynthesis,
systematic acquired resistance
AT5G22000
RHF2A (RING-H2 GROUP F2A); protein binding / zinc ion binding
(Vannini et al., 2006)
Sb05g022500
54441250
54442464
wound response
AT5G13930
TT4 (TRANSPARENT TESTA 4); naringenin-chalcone synthase
(Tohge et al., 2007)
Sb05g022510
54452544
54453758
wound response
AT5G13930
TT4 (TRANSPARENT TESTA 4); naringenin-chalcone synthase
(Yu et al., 2005)
Sb05g022800
55112819
55114699
defense response, apoptosis
AT1G58410
disease resistance protein (CC-NBS-LRR class) putative
(Ibraheem et al., 2010)
Sb05g022940
55294132
55295938
defense response to fungus
AT3G04720
PR4 (PATHOGENESIS-RELATED 4); chitin binding
(Narusaka et al., 2004)
Sb05g022950
55302124
55302693
defense response to fungus
AT3G04720
PR4 (PATHOGENESIS-RELATED 4); chitin binding
(Biruma et al., 2012)
Sb05g022960
55307197
55308168
defense response to fungus
AT3G04720
PR4 (PATHOGENESIS-RELATED 4); chitin binding
Sb05g023580
56306811
56311240
defense response, apoptosis
AT3G50950
disease resistance protein (CC-NBS-LRR class) putative
(Tan et al., 2007)
Sb05g023690
56425879
56427007
wound response
AT5G24090
acidic endochitinase (CHIB1)
(Lu et al., 2012)
Sb05g023700
56434322
56435584
wound response
AT5G24090
acidic endochitinase (CHIB1)
Sb05g023710
56447566
56448691
wound response
AT5G24090
acidic endochitinase (CHIB1)
Sb05g024020
56929808
56933159
defense response, apoptosis
AT3G14470
disease resistance protein (NBS-LRR class) putative
(Ashfield et al., 2004)
Sb05g024030
56937190
56942639
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
(Yang et al., 2006)
Sb05g024126
57066642
57069341
AT3G46710
disease resistance protein (CC-NBS-LRR class) putative
(Rose et al., 2004)
Sb05g024200
57225660
57227381
AT3G14470
57370289
57371722
(Hoffmann et al., 2003)
Sb05g024240
57379883
57386627
defense response to fungus
AT1G15520
disease resistance protein (NBS-LRR class) putative
HCT (HYDROXYCINNAMOYL-COA SHIKIMATE/QUINATE
HYDROXYCINNAMOYL TRANSFERASE)
PDR12 (PLEIOTROPIC DRUG RESISTANCE 12); ATPase coupled to
transmembrane movement of substances
(Nandety et al., 2013)
Sb05g024230
defense response, apoptosis
salicylic acid biosynthesis,
systematic acquired resistance
wound response, regulation of
flavonoid biosynthesis pathway
Sb05g024380
57568617
57570520
defense response to fungus
AT2G26560
PLA2A (PHOSPHOLIPASE A 2A); lipase/ nutrient reservoir
(Rietz et al., 2004)
(Vaid et al., 2012)
Sb05g024880
58055848
58059255
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
Sb05g024900
58076916
58080591
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
Sb05g024940
58124482
58125223
defense response
AT5G42510
58184326
58186026
cuticle development
AT5G43760
disease resistance-responsive family protein
KCS20 (3-KETOACYL-COA SYNTHASE 20); fatty acid elongase, stilbene
synthase
58333533
58337365
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
58724313
58729677
AT3G46730
disease resistance protein (CC-NBS class) putative
59089807
59098658
defense response, apoptosis
defense response, lignin
biosynthesis
AT1G65870
disease resistance-responsive family protein
Sb05g025040
1
AT5G48930
8
Sb05g025190
Sb05g025440
2
Sb05g025670
Sb05g025870
153
59350967
59354066
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
Sb05g025880
32
59362285
59365186
defense response, apoptosis
AT1G58400
disease resistance protein (CC-NBS-LRR class) putative
(Gechev et al., 2004)
(Ralph et al., 2007)
(Wang et al., 2004)
(Seo et al., 2007)
(Mun et al., 2009)
Supplemental Table S1. Sorghum genes annotated under the major anthracnose resistance QTL on sorghum chromosome 5. Genes shown are those that lie underneath the QTL interval where the LOD
score exceeded the 3.9 LOD significance threshold. Amino acid changes in genes associated with plant disease resistance were detected using resequencing data of parent SC748-5 in comparison to parent
BTx623. Arabidopsis orthologs were found using the Rice Genome Annotation Project (www. http://rice.plantbiology.msu.edu)
Sb05g026070
7
59692426
59693376
defense response
AT5G42510
disease resistance-responsive family protein
Sb05g026250
5
60044232
60046143
xenobiotic stress response
AT1G07530
SCL14 (SCARECROW-LIKE 14); transcription factor
Sb05g026260
18
60067359
60069221
AT1G07530
SCL14 (SCARECROW-LIKE 14); transcription factor
Sb05g026470
51
60360741
60364202
AT3G14470
disease resistance protein (NBS-LRR class) putative
Sb05g026480
118
60372348
60375425
xenobiotic stress response
salicylic acid biosynthesis,
systematic acquired resistance
salicylic acid biosynthesis,
systematic acquired resistance
AT3G14470
Sb05g026490
1
60376700
60377964
AT1G10360
(Kuśnierczyk et al., 2007)
Sb05g026540
1
60474285
60476244
AT5G20400
oxidoreductase 2OG-Fe(II) oxygenase family protein
(Davies, 1993)
Sb05g026570
1
60526640
60528039
AT4G08850
kinase
(Qi et al., 2011)
Sb05g026920
60938221
60940458
toxin catabolic process
encodes a flavonone-3hydroxylase protein
regulation of defense response,
systematic acquired resistance
salicylic acid biosynthesis,
systematic acquired resistance
disease resistance protein (NBS-LRR class) putative
ATGSTU18 (GLUTATHIONE S-TRANSFERASE TAU 18); glutathione
transferase
AT3G14470
disease resistance protein (NBS-LRR class) putative
Sb05g026930
60963661
60965513
defense response, apoptosis
AT1G50180
disease resistance protein (CC-NBS-LRR class) putative
Sb05g026965
61009529
61010503
defense response, apoptosis
AT1G50180
disease resistance protein (CC-NBS-LRR class) putative
Sb05g027000
61082967
61094282
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
Sb05g027090
61216936
61222508
defense response to fungus
AT1G21250
WAK1 (CELL WALL-ASSOCIATED KINASE); kinase
Sb05g027260
61490220
61499773
defense response, apoptosis
AT1G50180
disease resistance protein (CC-NBS-LRR class) putative
Sb05g027270
61505660
61506610
defense response, apoptosis
AT1G50180
disease resistance protein (CC-NBS-LRR class) putative
Sb05g027280
61515609
61524117
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
Sb05g027290
61529781
61530731
defense response, apoptosis
AT1G50180
disease resistance protein (CC-NBS-LRR class) putative
Sb05g027300
61569054
61572701
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
Sb05g027310
61588226
61589176
defense response, apoptosis
AT1G50180
disease resistance protein (CC-NBS-LRR class) putative
61589927
61591939
defense response, apoptosis
AT3G46730
disease resistance protein (CC-NBS class) putative
61657960
61659130
AT5G24090
acidic endochitinase (CHIB1)
61814780
61815331
AT3G53600
61831234
61832958
AT1G19640
zinc finger (C2H2 type) family protein
JMT (JASMONIC ACID CARBOXYL METHYLTRANSFERASE); jasmonate Omethyltransferase
(Wang et al., 2008)
Sb05g027465
wound response
defense response, response to
chitin
response to fungus, regulation
of hypersensitivity response
Sb05g027480
61868780
61869562
AT1G20030
pathogenesis-related thaumatin family protein
(El-kereamy et al., 2011)
Sb05g027620
62055349
62057361
defense response
salicylic acid biosynthesis,
systematic acquired resistance
AT3G14470
disease resistance protein (NBS-LRR class) putative
Sb05g027630
62073987
62075284
defense response
AT3G50950
disease resistance protein (CC-NBS-LRR class) putative
xenobiotic stress response
defense response, response to
chitin
AT1G07530
SCL14 (SCARECROW-LIKE 14); transcription factor
AT1G07520
scarecrow transcription factor family protein
Sb05g027320
3
Sb05g027380
Sb05g027450
2
Sb05g027740
1
62150295
62152965
Sb05g027760
4
62162581
62164664
(Fode et al., 2008)
(Tan andWu, 2012)
(Peleg-Grossman et al., 2012)
(Libault et al., 2007)
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