Additional files
Additional file 1 – supplementary table of PCR primers employed in this study
Primer Name
1911_Lup
1911_Ldwn
1911_Rup_Hom
1911_Rdwn
1923_Lup
1923_Ldwn
1923_Rup_Hom
1923_Rdwn
1924_Lup
1924_Ldwn
1924_Rup_Hom
1924_Rdwn
1925_Lup
1925_Ldwn
1925_Rup_Hom
1925_Rdwn
1926_Lup
1926_Ldwn
1926_Rup_Hom
1926_Rdwn
AcsA_Lup
AcsA_Ldwn
AcsA_Rup_Hom
AcsA_Rdwn
hmwp1_Lup
hmwp1_Ldwn
hmwp1_Rup_Hom
hmwp1_Rdwn
1925compFw
1925compRv
Sequence
GGGGCTCGAGGCCTTGCACACGCCGGATC
AGACGCACTGTCCAGCGTATCG
CGTCGATACGCTGGACAGTGCGTCTAGCGTGCCGGTGTTCGTG
CCCCTCTAGACTTGCGGTCAAGCTTGCC
GGGGGGATTCGATGTACGTGCGTAACTACGGC
GTTCATGGGTCGATCCTTGC
ATTCAGCAAGGATCGACCCATGAACGCATGACCGGGACCACCG
GGGGGGATCCTTCGGTTTCAGCACGGCCG
GGGGCTCGAGATGACCGGGACCACCGCT
AGCCAGACCGGACAAATCAATG
CAGCATTGATTTGTCCGGTCTGGCTCTGAAGACCGAGCTCCGTGC
CCCCTCTAGAGTCCAGCGCATGGTCACTTTC
GGGGGGAGCTCACAGTTGCTTGACTCCGTTA
GCACTTCCTGATCGACATAA
GAGCGTTATGTCGATCAGGAAGTGCAAACACTGCCCGATTACAT
GGGGGAAGCTTTTTTTCAATATCGGACAAGC
GGGGCTCGAGATGGACAAGTCTGCAGCAGAGC
TTCGATGTCGGCTGGCGC
CTCGGTTGCGCCAGCCGACATCGAAGTGGGGCTTATCGAAACCGC
CCCCTCTAGATCACACAAGCCGCTCGGC
GGGGCTCGAGATGAACTTCACTTCACTCGCCG
CTCCAGAAACAGCGCTATGCC
AAAGGGCATAGCGCTGTTTCTGGAGTGGCTTGAGCGTAACGGCA
GGGGTCTAGATCAGGTTGCACGGCGCAA
CCCCCTCGAGATGTACACATCGACCAGAAAAGC
CTGATACAGATGACAGCC
TGGAGTCGGCTGTCATCTGTATCAGCTGGCTGAGCAACCCCAG
CCCCTCTAGATCATGAACCACCTCCGCT
CCCCCATATGCAACAGTTGCTTGACTCCG
CCCCAAGCTTTCAGATACCCGCCATTTCATTC
Lup-Forward primer for amplification of 5’ gene fragment for in-frame gene knockout.
Ldwn-Reverse primer for amplification of 5’ gene fragment for in-frame gene knockout.
Rup-Forward primer for amplification of 3’ fragment for in-frame gene knockout; contains
25 bp homology region to corresponding 5’ fragment for SOE PCR (bold).
Rdwn-Reverse primer for amplification of 3’ gene fragment.
Restriction sequences are underlined and homology regions for SOE PCR are highlighted in
bold.
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