The CWRU Genomics Core Facility
Library Prep & NGS User Agreement Form
Simone Edelheit, Manager
BRB 747A
368-1887
sxw94@case.edu
Date: ______________________________________
Your Name: __________________________________
Primary Investigator: __________________________
Email: ______________________________________
Department: _________________________________ Account Number: _____________________________
Phone Number: ______________________________
Affiliation:
Project Title: ________________________________
Funding:
CWRU
NIH
CCCC
NSF
CTSC
CCF
OTHER
OTHER
1. Sample Submission:
Submit all samples in 1.5ml tubes, clearly labeled with sample name, the date, your name/initials and any QC data.
A. Organism: __________________ # of Samples: ________
B. Core Makes Library - Fill out Section 2
User-supplied DNA
User-supplied RNA
User-Supplied Library - Skip to Section 3 – Library Type / Company ________________________
2. Library Prep Services: (Skip to Section 3 if submitting User-supplied Library.)
DNA / Amplicon Libraries
TruSeq DNA PCR Free
Concentration
100ng/ul
Volume
20ul
QC
260/280: 1.8-2.0
Nextera Rapid Capture Exome
25ng/ul
20ul
260/280: 1.8-2.0
Nextera XT DNA
10ng/ul
20ul
260/280: 1.8-2.0
PCR product
Concentration
50ng/ul
10ul
Volume
20ul
Gel Image
QC
RIN: 7.0-10.0
10ng/ul
20ul
RIN 7.0-10.0
2-Step PCR (CRISPR)
RNA Libraries
TruSeq Stranded Total RNA (0.1 – 1000 ng)
Clontech Low Input Stranded RNA (10 – 100 ng)
PRICE/Library*
$250 (library #1-4)
$125 (library #5-24)
$250 (< 18 libraries)
$175 (> 18 libraries)
$70 / library for reagents
$50 / set of 48 processing
$10
PRICE/Library*
$250 (library #1-8)
$185 (library #9-96)
$250 (library #1-8)
$185 (library #9-24)
*Prices of library preps include all reagents, QC of initial & final library, and processing fees.
3. Sample Information: (If additional space is needed, provide an excel sheet with all information.)
# ID
Sample Name
Index
Volume
Given
Concentration
(if known)
Comments
1
2
3
4
5
6
7
8
Library Prep & NGS User Agreement Form, updated July 2015
The CWRU Genomics Core Facility
Library Prep & NGS User Agreement Form
Simone Edelheit, Manager
BRB 747A
368-1887
sxw94@case.edu
4. Sequencing Strategy: (Fill out Sections A thru E – see run options and processing fees* below.)
A.
HiSeq Rapid Run B.
HiSeq High Output
MiSeq
Single Read
Paired-end
C.
Multiplex Run D. ______ # total Runs E.
(pool samples)
______ # cycles (bp)
Custom Seq primer
Custom Index Primer
_________50ul @ 100uM
HiSeq 2500 – Rapid Run mode – v2 (300M max)
# of Cycles (bp)
Full flowcell (2 lanes)
Split flowcell (1 lane)
Split flowcell (2 lanes)
Single Read Run
50
$1,650
$1,025
$2,050
$2,300
$1,350
$2,700
Paired-end Run
100
10
50
$2,800
$1,600
$3,200
100
$3,600
$2,000
$4,000
250
50 cycle run (8 lanes)
$6,800
100 cycle run (8 lanes)
$3,600
125 cycle run (8 lanes)
$7,200
HiSeq 2500 – High Output mode – v4 (2 Bil max)
Single Read*
$7,000
$9,500
-
NO SINGLE LANE PRICES
Paired-end Read*
$12,000
-
$17,000
MUST FILL ALL 8 LANES
MiSeq
1x150 / 2x75 bp
1x300 / 2x150 bp
2x250 bp
2x300 bp
MiSeq v3 (25M reads)
$1,050
$1,700
$1,700
$1,700
MiSeq v2 (15M reads)
-
$1,100
$1,200
-
MiSeq Micro v2 (4M reads)
-
$1,000
-
-
MiSeq Nano v2 (1M reads)
-
$800
-
-
FINAL Library QC
Cost
Library QC (Qubit & DNA HS Bioanalyzer)
$14.00 / library
qPCR Analysis - Final Library Pool (incl Qubit)
$17.00 / library pool
MiSeq Library QC (2x26bp v2) – req. for HOP run
$800 / run
*require MiSeq Library QC run
*Sequencing prices include all Illumina sequencing reagents and Core processing fees. All final libraries are QC’d using the Agilent
Bioanalyzer DNA HS chip & KAPA Library Quantification kit. QC fees are separate.
5. Data Analysis, delivery format(s) and additional Information about the sample to be analyzed:
User Agreement:
Name
Signature
Date
Library Prep & NGS User Agreement Form, updated July 2015