The Norwegian High Throughput Sequencing Centre
Sample submission form
ILLUMINA SEQUENCING
INSTRUCTIONS
Fill out ALL boxed sections (light grey, sections 1 - 7), then save with a filename of your
choice (recommended to include name and date) and return to us, as a .doc or .docx file
(NOT .pdf), by email to ous-seq@sequencing.uio.no
PLEASE READ the accompanying guideline file, available here:
http://www.sequencing.uio.no/forms/samplesubmissionforms.html
This contains essential information on sample quality and amounts required, in addition to
advice on completing the following sections. Submissions not fulfilling the criteria cannot
be sequenced.
REQUIRED ATTACHEMENTS
Evidence of sample size & quality (gel image or bioanalyzer results) is mandatory unless
agreed in advance. We regret we can not perform these analyses on your behalf. We may
verify sample quality upon receipt, and will contact you if they do not meet our
requirements.
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1. General Sample Information (ALL fields mandatory)
Method used to purify DNA / RNA
Click here to enter text.
(e.g. Phenol / Chloroform & EtOH precip.)
Method used to determine concentration
Click here to enter text.
(e.g. Nanodrop / Qubit etc.)
Click here to enter text.
Buffer in which samples dissolved (see
guidelines for restrictions). Please also
provide a sample.
Yes
No
Are samples hazardous (if yes, give details)?
Click here to enter text.
Are the samples ready to sequence?
Yes, they are Illumina-compatible libraries I
prepared myself.
If yes, please state which kit / method you used
here: Click here to enter text.
No, these are DNA / RNA samples. I would like
the NSC to perform sample prep.
2. Sample prep requests (mandatory if NSC is to perform sample prep)
For DNA samples:
(pick one)
Regular TruSeqTM Nano adapter ligation
TruSeqTM PCR-free prep
Tagmentation (Nextera XTTM) sample prep
I´m unsure, please advise
For RNA samples:
(pick one)
Strand-specific TruSeqTM RNA-seq library prep
small RNA library preparation
I´m unsure, please advise
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3. Sample Information table (Fields in red are mandatory)
Please complete the table, one line per sample. Add extra rows as required by right mouse click -> Insert -> Insert rows above/below.
If you have more than 16 samples, please use our excel template file, available here: http://www.sequencing.uio.no/forms/
Sample Name
Type*
(MAX 12 characters,
only letters, numbers
and hyphen allowed)
example
gDNA
Conc.
A260
(ng/µl) /280

21.0
1.79
A260
/230
1.79
Volume
provided
(µl)
95
Total DNA
/ RNA (µg)
2
Index
(barcoding)
used or
requested §
index together
with sample 2
Primers,
Linkers or RE
sites present?
†
EcoRI digest.
All reads start
AATTC
Approx no.
Reads, Gb or
lanes
requested
1 Lane
Comments
Indexed
(pool of 2)
10 nM
library ready
to sequence
1
2
3
4
5
6
7
8
Please also include a sample (~10 µl) of the elution buffer in which your sample is dissolved
* / § / † See guidelines for accepted definitions
 Restrictions apply - consult accompanying guidelines
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4. Sequencing Requested (ALL fields mandatory):
Species:
Reference genome & release version:
Sequencing type:
Desired insert size DNA sample and paired
end reads: (default 350 bp)
Sequencing Instrument requested:
(leave blank if unsure)
Read Length:
(reagents stocked as standard in bold)
H = HiSeq
M = MiSeq
N = NextSeq
Total number lanes (HiSeq) or runs (MiSeq /
NextSeq) requested:
Project Goal (brief description):
REK approval number if sequencing human
samples:
Click here to enter text.
Click here to enter text.
Single Read
Paired End
Click here to enter text.
HiSeq 2500, high output mode
HiSeq 2500, rapid mode
NextSeq 500 (mid output reagents)
NextSeq 500 (high output reagents)
MiSeq
40 bp (N – high output paired end only)
50 bp (H, M, H-rapid)
75 bp (N – high output single read only)
100 bp (H-rapid)
125 bp (H, high output)
150 bp (M, N)
250 bp (M, H-rapid)
300 bp (M)
Click here to enter text.
Click here to enter text.
Click here to enter text.
5. Select Data delivery format (Select one - mandatory):
Data will be stored at our local storage facility for a period of 2 months from the date of the
delivery mail after which it will be permanently deleted. A reminder notice will be sent to
the contact email address two weeks prior to deletion.
Upload to https site hosted by Norstore (option only available for non-sensitive
samples). We will send you the necessary information to download the data in the
delivery mail.
Portable hard drive (exFAT format) to be picked-up from Ullevål by user (primarily for
human samples).
You may choose whether you wish to deliver your own portable hard drive to NSC
or
whether NSC should purchase a portable hard drive for you (you will be charged for
this at approx. 1,000 NOK per hard drive).
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6. Contact & Billing details (All fields mandatory):
Forms submitted without a valid purchase order number (or internal Kontostreng for OUS
projects) will be returned to you for completion. Please contact us if you require a price
estimate in order to obtain this number from your institute. We will not initiate your project
until this has been completed.
Submission Contact Information
Contact Name*:
Institution:
Address:
(including postcode)
Click here to enter text.
Click here to enter text.
Click here to enter text.
Email:
Telephone:
Click here to enter text.
Click here to enter text.
* This person will be our contact should we have any questions regarding sample
preparation. They should also be prepared to act as the single point of contact for their
research group for this project with respect to delivery of data and billing.
Billing Details
Institution:
Billing contact person:
Email:
Telephone:
Billing Address:
Click here to enter text.
Click here to enter text.
Click here to enter text.
Click here to enter text.
Click here to enter text.
Postcode:
Purchase Order Number
Click here to enter text..
Click here to enter text..
Project is fully or partly funded by The Research Council of Norway
(Norsk Forskningsrådet)?
Yes
No
(required for statistics and reporting purposes only)
Internal orders (Oslo Universitetssykehus) only:
Kontostreng:
(Art-KoststedProsjektnummer-Kilde)
Click here to enter text.
Note that we do not send multiple bills for a submission. Users must provide a single source
of payment.
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7. Sample Delivery Information
Delivery in person
Delivery by carrier
Carrier:
Click here to enter text.
Tracking number: Click here to enter text.
Deliver / ship samples to:
Kristine Fjelland / Gregor Gilfillan / Robert Lyle
NSC Illumina Node,
Medisinsk Genetikk,
Lab. bygget 2. etasje (Nord)
Oslo Universitetssykehus (Ullevål)
Kirkeveien 166
0407 Oslo
Contact Telephone: +47 2211 9724 / 2211 9860
Email completed form to: ous-seq@sequencing.uio.no
User Checklist:
Samples
Sample buffer sample (~ 10µl)
Completed Submission form
Sample table (Excel file if >24 samples)
Gel image / Bioanalyzer trace
DNA: Send with -20 oC
cooling blocks.
RNA: Send on dry ice.
Return by email
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