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Figure S7. Testing specificity of multiplex primer mix and uniplex primer mix vs.
Saccharomycetaceae.
The Saccharomyces species-specific primers were tested for specificity
against a panel comprising 15 strains representing 13 groups of the
Saccharomycetaceae, and type strains of six Saccharomyces species. A multiplexed
mix of all primers was tested against these strains as well as the individual primer
pairs in separate reactions.
L
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10 11 12 13
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20 21 22
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Figure S7a. S. bayanus primer mix in PCR reactions with members of the
Saccharomycetaceae::
1 – T. delbrueckiiNT CBS1146, 2 – T. microellipsoidesT NCYC 2568, 3 – Zt.
florentinusT NCYC 2513, 4 – C. glabrataT CBS 138, 5 – H. uvarumT CBS 314, 6 – L.
fermentatiT NCYC 2508, 7 – Z. rouxiiT CBS 732, 8 – Tp. blattaeT CBS 6248, 9 –
Naumovozyma dairenensis NCYC 1474, 10 – S’mycodes ludwigiiT CBS 821, 11 –
Kazachstania exiguaNT CBS 379, 12 – Vanderwaltozyma yarrowiiT NCYC 2754, 13
– H. osmophilaT CBS 313, 14 – Kl. marxianusT CBS 712, 15 – E. coryliT CBS2608,
16 – S. cerevisiaeT, 17 – S. bayanusT, 18 – S. arboricolaT, 19 – S. mikataeT, 20 – S.
kudriavzeviiT, 21 – S. paradoxusT, 22 – PCR negative, L – 100bp ladder.
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Figure S7b. S. cerevisiae primers in PCR reactions with members of the
Saccharomycetaceae:
1, 16 & 17 – 100bp ladder, 2 – T. delbrueckii, 3 – Z. microellipsoides, 4 – Z.
florentinus, 5 – C. glabrata, 6 – H. uvarum, 7 – Z. fermentati, 8 – Z. rouxii, 9 – T.
blattae, 10 – N. dairenensis, 11 – S. ludwigii, 12 – Ka. exigua, 13 – V. yarrowii, 14
– H. osmophila, 15 – K. marxianus, 18 – E. coryli, 19 – S. cerevisiae, 20 – S.
bayanus, 21 – S. arboricola, 22 – S. mikatae, 23 – S. kudriavzevii, 24 – S.
paradoxus, 25 – PCR negative.
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Figure S7c. S. arboricolus primer mix in PCR reactions with members of the
Saccharomycetaceae:
1, 16, 17 & 26 – 100bp ladder, 2 – T. delbrueckii, 3 – Z. microellipsoides, 4 – Z.
florentinus, 5 – C. glabrata, 6 – H. uvarum, 7 – Z. fermentati, 8 – Z. rouxii, 9 –
T. blattae, 10 – N. dairenensis, 11 – S. ludwigii, 12 – Ka. exigua, 13 – V.
yarrowii, 14 – H. osmophila, 15 – K. marxianus, 18 – E. coryli, 19 – S.
cerevisiae, 20 – S. bayanus, 21 – S. arboricola, 22 – S. mikatae, 23 – S.
kudriavzevii, 24 – S. paradoxus, 25 – PCR negative.
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Figure S7d. S. mikatae primer mix in PCR reactions with members of the
Saccharomycetaceae:
1, 16, 17 & 26 – 100bp ladder, 2 – T. delbrueckii, 3 – Z. microellipsoides, 4 – Z.
florentinus, 5 – C. glabrata, 6 – H. uvarum, 7 – Z. fermentati, 8 – Z. rouxii, 9 – T.
blattae, 10 – N. dairenensis, 11 – S. ludwigii, 12 – Ka. exigua, 13 – V. yarrowii,
14 – H. osmophila, 15 – K. marxianus, 18 – E. coryli, 19 – S. cerevisiae, 20 – S.
bayanus, 21 – S. arboricola, 22 – S. mikatae, 23 – S. kudriavzevii, 24 – S.
paradoxus, 25 – PCR negative.
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Figure S7e. S. kudriavzevii primer mix in PCR reactions with members of the
Saccharomycetaceae:
1, 16, 17 & 26 – 100bp ladder, 2 – T. delbrueckii, 3 – Z. microellipsoides, 4 – Z.
florentinus, 5 – C. glabrata, 6 – H. uvarum, 7 – Z. fermentati, 8 – Z. rouxii, 9 – T.
blattae, 10 – N. dairenensis, 11 – S. ludwigii, 12 – Ka. exigua, 13 – V. yarrowii, 14
– H. osmophila, 15 – K. marxianus, 18 – E. coryli, 19 – S. cerevisiae, 20 – S.
bayanus, 21 – S. arboricola, 22 – S. mikatae, 23 – S. kudriavzevii, 24 – S.
paradoxus, 25 – PCR negative.
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Figure S7f. S. paradoxus primer mix in PCR reactions with members of the
Saccharomycetaceae:
1, 16, 17 & 26 – 100bp ladder, 2 – T. delbrueckii, 3 – Z. microellipsoides, 4 – Z.
florentinus, 5 – C. glabrata, 6 – H. uvarum, 7 – Z. fermentati, 8 – Z. rouxii, 9 –
T. blattae, 10 – N. dairenensis, 11 – S. ludwigii, 12 – Ka. exigua, 13 – V.
yarrowii, 14 – H. osmophila, 15 – K. marxianus, 18 – E. coryli, 19 – S.
cerevisiae, 20 – S. bayanus, 21 – S. arboricola, 22 – S. mikatae, 23 – S.
kudriavzevii, 24 – S. paradoxus, 25 – PCR negative.
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