Ashley Chan (Peterson) 3181 Margie Ave SLC, UT 84109 (801) 910-9735 ashl.chan@gmail.com EDUCATION Bachelor of Science Degree, Biology University of Utah (Spring 2006) Undergraduate Laboratory Concentration: Genetics under Dr. Jon Seger Member of BioScience Undergraduate Research Program Secretary of BioScience Undergraduate Research Club Teaching Assistant of Basic Laboratory Technique 2115 Presented research results at National Conference for Undergraduate Research PROFESSIONAL EXPERIENCE University of Utah—SLC, UT Senior Laboratory Specialist/Lab Manager, Dr. Joshua Schiffman Jan 2012-present University of Utah—SLC, UT Temporary Laboratory Technician, Dr. Ila Singh Sept 2011-Dec 2012 SuperGen—SLC, UT Contract Research Associate II, Dr. Jason Foulks May 2011-Sept 2011 Myrexis (Myriad) Pharmaceuticals—SLC, UT Research Associate II, Dr. Daniel Cimbora February 2008-March 2011 Myriad Genetics—SLC, UT Laboratory Technician II, James McCulloch January 2006-February 2008 University of Utah—SLC, UT & HNL, HI Research Assistant, Dr. Coleen Farmer/Dr. Ben Chan May 2005-December 2005 University of Utah—SLC, UT Laboratory Technician, Dr. Jon Seger May 2003-May 2005 RELEVANT LABORATORY EXPERIENCE Histological Immunohistochemistry FFPE sectioning (microtome) Biochemical and Molecular Biological PCR, q-PCR, RTPCR Sequencing Gel Electrophoresis—SDSPAGE, agarose gel Macromolecule Blotting/Probing—Southern, Northern, Western Protein/NA isolation and purification Radiometric (33P) & ADP Glo assays Electroporation Immunofluorescence Immunoprecipitation Flow Cytometry ELISA Immuno/Physiological Cell culture—bacterial, mammalian, primary, stem cell Stem Cell Differentiation Cellular cloning Transfection—liposome Xenograft preparation Cytotoxicity, proliferation Phenotypic Assays—2D Colony, luciferase, fluorescence Mycoplasma Testing Ecological Behavioral Observations Collection Managerial Supply inventory & ordering Developing assays & protocols Budgeting Interviewing Training IACUC/IRB protocols Abstract/paper/grant writing, proof reading & submission Robotic Operations & Maintenance Liquid Handlers Sequencers PCR NA isolators Chemiluminescent Microplate Readers Spectrophotometers Flow Cytometers Data Analysis R programming (some) Microsoft Office Copy Number Prism Graph Sequencher MacClade In-house Analyzation Software Training & Certifications OSHA BSL2 IRB HIPPA Radioactive Bloodborne Pathogen Good Laboratory Practices SDS ADDENDUM 1 PUBLICATIONS Peterson, A.L., Purwar, J., Mason, C., Cairns, B., Schiffman, J.S. Paraganglioma cell lines: A novel clinical model for mutations within the succinate dehydrogenase complex. (in prep) Purwar, J., Pflueger, C., Srinivasan, R., Chan, A.L., Wysocka, J., Ellis, G., Schiffman, J.D., Cairns, B.R. Profiling epigenetic misregulation in SDH mutated PGLs when compared to cell type of origin, neural crest cells. (in prep, submission planned Dec. 2013) Caulin, A., Peterson, A.L., Bharaska, S.V., Schiffman, J.S., Malley, C. The Elephant’s Solution to Peto’s Paradox: Go Forth and Multiply TP53. (in prep submission planned imminently) Peterson, A.L., Mason, C., Jaromi, M., Purwar, J., Tripp, S., Ellis, G., Stewart, R., Cairns, B., Schiffman, J.S. CNV in paraganglioma tumors with known SDH mutations. (in prep) Keith D. Tardif, Aaron Rogers, Jared Cassiano, Bruce L. Roth, Daniel M. Cimbora, Rena McKinnon, Ashley Peterson, Tom Douce, Rosann Robinson, Irene Dorweiler, Ryan Doering, Thaylon Davis, Kirill Ostanin, Damon I. Papac, Vijay Baichwal, Ian McAlexander, J. Adam Willardsen, Michael Saunders, Christophe Hoarau, D. Vijay Kumar, Daniel A. Wettstein, Robert O. Carlson, Brandi L. Williams. Characterization of the Cellular and anti-Tumor Effects of MPI-0479605, a Small Molecular Inhibitor of the Mitotic Kinase MPsl. Mol Cancer Ther. 2011 Oct Bursavich, M.G., Parker, D.P., Willardsen, A., Gao, Z., Davis, T., Ostanin, K., Robinson, R., Peterson, A.L., Cimbora, D.M., Zhu, J., Richards, B. 2-anilin-4-aryl-1,3-thiazole inhibitors of valosin-containing protein (VCP or p97). Bioorg Med Chem, 2010 Mar 1;20(5):1677-9 Roeser-Mueller, K., Peterson, A.L., Strohm, E., Stubblefield, J.W., Seger, J., Kaltenpoth, M. Phylogentic relationships among Philanthinae (Hymenoptera: Sphecidae). (in review PLOS) Chan, B. K., Peterson, A.L., and C. G. Farmer. 2007. Dendrobates auratus (Green and Black Poison Dart Frog) Larval Predation. Herpetological Review 38(3): 321-322 Chan, B.K., Peterson, A.L., Farmer, C. Post-depositional paternal care of larvae in a tropical anuran. Herpetological Review (in prep) POSTERS Chan, A.L., Caulin, A.F., Bhaskara, S., Peterson, E., Maley, C.C., Schiffman, J.D. Peto’s Paradox: Is increased apoptosis the answer to cancer resistance? Purwar, J., Pflueger, C., Srinivasan, R., Chan, A.L., Wysocka, J., Ellis, G., Schiffman, J.D., Cairns, B.R. Profiling epigenetic misregulation in paragangliomas Hawkins, S., Chan, A.L., Abegglen, L., Jones, D., Burt, R., Mason, C., Schiffman, J.D. Copy Number Analysis to determine genetic alterations in paired primary and metastatic colorectal cancer samples Foulks, J.M., Xu, Y., Saunders, M., Liu, X.H., Brenning, B., Clifford, A., Wilkes, M., Luo, B., Lai, S., Merx, S., Chan, A., Huang, L., Vollmer, D., Senina, A., Liu, J., Ho, K., McCullar, M.V., Kanner, S.B. Second generation PIM inhibitors exhibit improved activity in solid tumor models Boniface, J.J., Baichwal, V.R., Cimbora, D.M., DeMie, L., Fleischer, T.C., Gassman, A.D., Ghaffari, S., Green, J.P., Flick, J.S., Terry-Lorenzo, R.T., Papac, D.I., Peterson, A.L., Robinson, R., Carlson, R.O. Basal NAD levels and Nampt expression correlates with in vitro and in vivo sensitivity of tumor cell lines to the Nampt Inhibitor MPC-9528 Fleischer, T.C., Baichwal, V.R., Boniface, J.J., Cimbora, D.M., DeMie, L., Douce, T.B., Gassman, A.D., Papac, D.I., Peterson, A., Robinson, R., Terry-Lorenzo, R.T., Carlson, R.O. Coadministration of nicotinic acid with the Nampt inhibitor MPC-9528 enhances anti-tumor activity in Naprt deficient cancer cells in culture and in xenografts Boniface, J.J., Baichwal, V.R., Bradford, C.L., Peterson, A.L., Cimbora, D.M., Demie, L., Flick, J.S., Gassman, A.D., Gordillo, R., Fleischer, T.C., Green, J.P., Lockman, J.W., Murphy, B.R., Papac, D.I., Robinson, R., Terry-Lorenzo, R.T., Willardsen, J.A., Carlson, R.O. MPC-9528, a cancer metabolism inhibitor, demonstrates greater therapeutic index in a Naprt1 deficient cancer xenograft model with co-administration of nicotinic acid Richards, B., Cronin, M., Seager, N., Niederjohn, J. Baichwal, V. Peterson. A.L., Robinson, R., Hess, M., Davis, T. Papac, D. Suzuki, K., Holcomb, R., Carlson, R. Cellular and In Vivo Properties of MPI-0485520, a Novel and Potent Small Molecule Inhibitor of IKKε AWARDS BioScience Undergraduate Research Program Minigrant to study Phylogentic relationships among Philanthinae. Total award: $14,480, (5/03-5/05) ADDENDUM 2 REFERENCES Joshua Schiffman, Director, Medical Director/Associate Professor at Huntsman Cancer Institute Joshua.schiffman@hci.utah.edu or 801-587-4745 Dan Cimbora, Clinical Research Scientist at Myrexis Pharmaceuticals dlcimbora@comcast.net or 801-485-3477 Harry Austin, (formerly) Scientist II at Myrexis Pharmaceuticals harry_austin09@comcast.net or 801-214-7800 Jason M. Foulks, Senior Manager of Discovery Biology at Astex Pharmaceuticals (formerly SuperGen Inc) Jason.foulks@astx.com or 801-386-2974 Scott Morham, (formerly) Director of Research Services at Myrexis Pharmaceuticals Vaccinia@comcast.net Mark Wolf, Supervisor at Myriad Genetics mwolf@myriad.com Dr. Jon Seger, Professor of Biology at University of Utah seger@biology.edu or 801-581-4758