1
90
ATG GGA ATA ACT AAG CAG AAA TTA GGA AAT GAG CAC GAA ATG TCT ATA AAT GAA TTT TCT CAT TAT TTG TTA TTT TCG GGT CTT TTC GTT
M
G
I
T
K
Q
K
L
G
N
E
H
E
M
S
I
N
E
F
S
H
Y
L
L
F P>S G
L
F
V
2
3 180
GCA TTC ACT TAC AAC AAG AAA CAA CCA CCA GCG TTT GGT GCA GCA CTT GCA TTT TGG TGC ATT CTT CTT TCT TTC CTT GGT CTT TTG TTC
A
F
T
Y
N
K
K
Q
P
P
A
F
G
A
A P>L A
F
W
C
I
L
L
S
F
L
G
L S>L F
4
5
270
TGT CAT ATT TCA AAT AAC TTA TCT AAT TAC AAC GTA TTA ACC GCT AAT GCA CCT TTT TTT TAT CAA ATC TCA GGG ACA TGG TCT AAT CAT
R>C H
I P>S
N
N
L
S
N
Y
N
V
L
T
A
N
A
P
F
F
Y
Q
I
S
G
T
W
S
N
H
6
7
360
GAG GGT AGT ATT TTC TTA TGG TGT TGG ATC CTA AGT TTT TAT GGA TTC CTT TTT TGT TAC CGG TAC CGG GGT CGA CCC CAA AGC CAT AAT
E
G
S
I
F S>L W
C
W
I P>L
S
F
Y
G
F
L
F
C
Y
R
Y
R
G
R
P
Q
S
H
N
8
9
10
450
GTC TCA AAA CGC AGA GGC TAT AGA GAA ACT TTT ATT TTT TTC TTT GTC TTG AAC TTT GTG AAG AAC TCC ATT CTA TCT CTC CAA CAA AAA
V
S
K
R
R
G
Y
R
E
T
F
I
F S>F F
V S>L N
F
V
K
N
S
I
L
S
L
Q
Q
K
540
AGT GGA GCT GCG CCC CAG TTG TAC ACT CCC TTC GTT CGG AGA ACC CTT GTT GAT TCT GAA CTT CGT TCG CAA AGT AAG CGT CCT TTT AAC
S
G
A
A
P
Q
L
Y
T
P
F
V
R
R
T
L
V
D
S
E
L
R
S
Q
S
K
R
P
F
N
11
630
GGG CCA GCC CTT TTT AAT GCG CCG CTT GAC CCA GTT TTG AAA ATG AGC TTT GCT TTT CTG GGC GCT GGG CGC TCC CGT GGT TCG CGA GAA
G
P
A
L
F
N
A
P
L
D
P
V
L
K
M
S
F
A L>F L
G
A
G
R
S
R
G
S
R
E
720
GGA AAA AGG ACT AAT CTT TTG TTA CAT CTG GCA CGA GAT GAA AAA GAG AGA GCT TCG TCT ATC GAT GAA CAG CAG ATT GAC GGA GCT CTT
G
K
R
T
N
L
L
L
H
L
A
R
D
E
K
E
R
A
S
S
I
D
E
Q
Q
I
D
G
A
L
12
13
14
15
810
GGC ATT GCT TTG TTT TTC TCT CCT TTT CTA TTA GCG AGT TCT GAT CCT TTT GTT CGA AAT TTC TTC GTT TGT ACC GAA CCG CTT GCA GAA
G
I
A
L
F
F
S
P
F
L S>L A
S
S
D
P
F
V
R
N
F
F
V R>C
T
E
P
L
A
E
16
17
18
900
TTA AAT CCT GTT CTA CAA GAT CCT ATA TTA GCT ATA CAT CCT CCT TGC ATT TAT GCC GGA GAC GTC GCC AGT GCT ATG GGC TTT GGG TTA
S>L N
P
V P>L Q
D
P
I S>L A
I
H
P
P
C
I
Y
A
G
D
V
A
S
A
M
G
F
G
L
19
TGT AGA TCA AAA ATG ATG AAT GGG ATT GTG GCA CTC CAC TCG CCG CCA ATG CGG AAG GAT GCC GCC GAA AAG AAT GGA ACG CTG CTT TGC
C
R
S
K
M
M
N
G
I
V
A
L
H
S
P
P
M
R
K
D
A
A
E
K
N
G
T
L
L R>C
1080
TCT GCT GGA TGC GTC GGA TCC CAT ATA AGA AGC TCG CTC TTT ACC CGA TCA TTC AAA CAT TTT GTG GGC GGC GCG CCT GCT CTA TTG TTG
S
A
G
C
V
G
S
H
I
R
S
S
L
F
T
R
S
F
K
H
F
V
G
G
A
P
A
L
L
L
1170
CGT AGC AAT AGA AGC CTG CTT CGG CGG CGC TTT TTC GCC TTC TCT TCG CTC TGG ACA GGA GCG CTA ATG GAC ACG GGG AGG GAG CAG GCG
R
S
N
R
S
L
L
R
R
R
F
F
A
F
S
S
L
W
T
G
A
L
M
D
T
G
R
E
Q
A
1260
AAG CGT GTT GTC GTT CGT AAT GGA AAG AAA GAC ACC ACT ACT TCG CCT CTT TGT TGG ACC GCC GGC GCG AAC ACA GTG GTC TCT GAC CAG
K
R
V
V
V
R
N
G
K
K
D
T
T
T
S
P
L
C
W
T
A
G
A
N
T
V
V
S
D
Q
20
21
1350
GAC CAG GAA CCA ATT CGA ATT TGG ATC TTG ACA TGT TGG TTG TTT TTA ACC GTA GGC ATC TCG CTA GGA AGT TGG TGG GCT TAT CAT GAA
D
Q
E
P
I
R
I
W
I
L
T
C
W
L
F
L
T
V
G
I
S P>L G
S
W
W
A H>Y H
E
22
23
24
1440
TTA GGT TGG GGT GGC TGG TGG TTT TGG GAT CCC GTA GAA AAT GCG TCT TTT ATG CCT TGG GTA TTA GCC ACA GCT CTT ATT CAT TCA GTA
L
G R>W G
G
W
W
F R>W D
P
V
E
N
A
S
F
M
P R>W V
L
A
T
A
L
I
H
S
V
25
26
27
28
1530
ATT CTA CCC CTT CTT CAT TAT TGG ACC TTG CTT CTG AAT ATT TTG ACT TTT CTA TGC TGT GTC TTA GGA ACC TTT TCA ATA CGG TCC GGA
I
L
P
L
L
H
Y
W
T S>L L
L
N
I
L
T L>F P>L C
C
V S>L G
T
F
S
I
R
S
G
29
30
31
1620
TTG CTA GCT TCC GTT CAT AGT TCT GCT ACA GAC GAT ACA CGA GGA AGA TTT TTA TGG TGG TTC TTC CTT CTA ATT ACA GGC ATA TCT ATG
L
L
A P>S V
H
S
S
A
T
D
D
T
R
G
R
F
L
W R>W F
F
L
L
I
T
G
I
S
M
1710
ACT CTT TTT TAC CAG ATG AAG CAG GTC CGT AGA ACC TAT AAA AAA GAG ATG GTT GTG GCG CGA AGT ACT CTT GTG CAC CTA CGT CAC CCG
T
L
F
Y
Q
M
K
Q
V
R
R
T
Y
K
K
E
M
V
V
A
R
S
T
L
V
H
L
R
H
P
1800
GCT CGC GCG CAA CCC CGC CCC GTT ATG TTA TGG AAG AAT TTA GCT TCT TGC TGG GCT GGT TAT TCC GAG CCA GCA ACT GGC TGG CTG CCG
A
R
A
Q
P
R
P
V
M
L
W
K
N
L
A
S
C
W
A
G
Y
S
E
P
A
T
G
W
L
P
1863
GAT TTC GTC CCG TCC GTA GCG CTT CGG AAG TCA CTC TGG CGT GGC GCT GCT GGA TAC TTC TGA
D
F
V
P
S
V
A
L
R
K
S
L
W
R
G
A
A
G
Y
F
*
Figure S8. Nucleotide sequence of ccmFN cDNA from emp7 mutant. The deduced amino acid sequence is
shown under the cDNA sequence. Edit sites (T resulting from C editing) are shown in red and numbered.
Modifications in the amino-acid sequence resulting from RNA editing are indicated. The non-edited cytidine in
emp7 mutant is highlighted in yellow. Conserved histidine residues and WWD domain in bacterial and
mitochondrial CcmF proteins are highlighted in green.