Table S9. Selected sequencing Primers designed for 18S, 28S, 12S&16S, and EF-1α. Primer name lists
the following information: Gene, fragment, direction, year, series letter. UP = Forward Primer. DN =
Reverse Primer. Amplicon length is approximate and for EF-1α includes introns.
Primer Name
Primer Sequence
5’- 3’
Amplicon Length
18sV4UP95a
18sV4DN95a
CGT ATA CTA AAG TTG TTG CGG
GCC CTT CCG TCA ATT CCT TT
610bp
28sD1UP95b
28sD1DN95a
AGG GGA GGA AAA GAA ACT AAC
CAA CTT TCC CTC ACG GTA CT
345bp
28sD2UP95g
28sD2DN95d
GCT TGA GAG TGC AGC CCA
CCC AAC GAC TYG CGC GCA TGT T
660-730bp
28sD3UP95a
28sD3DN95a
ACC CGT CTT GAA ACA CGG AC
CGG AGG GAA CCA GCT ACT AGA TG
300-310bp
28sD46UP95a
28sD46DN95a
TGG GTA TAG GGG CGA AAG AC
TTG CTA CTA CCA CCA AGA TCT
595-675bp
28sD7UP95a
28sD7DN95a
GCS GCG ACG AGT AGG AGG G
CTT CAG AGC CAA TCC TTA T
645bp
28sD8UP96b
28sD8DN96c
GGT AAG GGA AGT CGG CAA
GCT CCC ACT TAT GCT ACA C
615bp
28sD10UP96b
28sD10DN96a
GAA AGA AGA CCC TGT TGA GC
GGC CGC CAC AAG CCA GTT AT
600bp
28sD12UP96b
28sD12DN96e
28sD12DN97b
GAG GTG TCA GAA AAG TTA C
ATT CCG AGA CCC GAC GCC G
CCG GTA CTY AAG TCG T
600bp
625bp
12sM1UP97c
12sM1DN97a
12sM1DN97d
GAC AAA ATT CGT GCC AGC
TGA GAG CGA CGG GCG AT
TAC TAT GTT ACG ACT TAT C
560bp
590bp
16sM2aUP97a
16sM2bDN97d
AAA UAU GCA CAY AUC GCC
GGU AGC AUA AUC AUU AGU CT
910bp
16sM2aUP97a
16sM2aDN97a
AAA UAU GCA CAY AUC GCC
ATA GAG CTT ATC CCC TA
565bp
16sM2bUP97b
16sM2bDN97d
GGT ATC TRG TTT TTY AAG
GGU AGC AUA AUC AUU AGU CT
450bp
16sM3UP97b
16sM3DN97a
GAA GTC TGA CCT GCC CRG TG
CGC CGG TCT GAA CTC AGA TCA TGT A
535bp
EF1UP97a
EF1DN97a
TCC TTC AAG TAY GCN TGG GT
CCR ATC TTC TTR ATR TAR TT
400bp
EF2UP97a
EF2DN97a
GGA GAR TTY GAA GCY GGT ATY TC
TCA ACR GAY TTR ACY TCA GT
520bp
EF3UP97a
EF3DN97a
ATY GGT GGT ATY GGA ACR GT
CCA CRC ACA TGG GCT TGC T
460bp