Score
1
2
3
Retinal Tissue
Optic Disc
Retinal vessel
Infiltrates
inflammation
cuffing
1-4 small lesions
or 1 linear lesion
Minimal
Moderate
Engorged vessels
with no perivacular
cuffing
Engorged vessels
and 1-4 mild
cuffings
>4 mild cuffings or
5-10 small
lesions or 2-3
linear lesions
>10 small lesions
or >3 linear
lesions
Mild
inflammation
1-3
inflammation
inflammation
moderate
cuffing
4
Linear lesion
Severe
confluent
inflammation
>3 moderate
cuffings or >1
severe cuffing
Structural damage
Retinal lesions or retinal
atrophy < 1/4 retinal area
Retinal lesions or retinal
atrophy involving 1/4 to 3/4
retina area
Pan retinal atrophy with
multiple small lesions (scars) or
<3 linear lesions (scars)
Pan-retinal atrophy with >3
linear lesions or confluent
lesions (scars)
Supplementary Table 1. Clinical grading criteria in C57BL/6 EAU mice. The clinical
inflammation score is separated from the structural damage score. Inflammation score is the average
score of retinal tissue infiltrates, optic disc inflammation and retinal vessel cuffing.
Supplementary Figure 1. Clinical evaluation of retinal inflammation at different stages of
EAU in WT, CCL2 KO and CX3CR1 KO mice. EAU was induced in WT, CCL2 KO, and
CX3CR1 KO mice. Fundus images were acquired by TEFI at different stages of EAU. A,
representative fundus images from control non-immunized mice and EAU mice at days 25, 60 and
90 p.i. B, clinical inflammation score (papillitis, vasculitis and infiltrates) in WT and DKO EAU
mice at different stages of EAU. C, clinical structural damage score (retinal atrophy, the number
and size of retinal lesion) in WT and DKO EAU mice at different stages of EAU.
Supplementary Figure 2. Retinal angiogenic membrane in different strains of day 90 p.i. EAU
mice. EAU was induced in WT, CCL2 KO, CX3CR1 KO and CCL2-/-CX3CR1 gfp/gfp mice. At day
90 p.i., mice were sacrificed and eye collected. Retinas were dissected and processed for flatmount
staining with collagen IV antibody. Samples were examined by confocal microscopy. A,
representative images showing angiogenic membrane (arrows) in different strains of EAU mice. B,
the percentage of reduction in the total area of retinal angiogenic membrane in DKO, CCL2 KO,
and CX3CR1 KO mice related to WT EAU mice. Data were presented as mean ± SEM, n = 5.
**P<0.01, unpaired Student t test.