BIOSCIENCES RESEARCH PRECINCT MOLECULAR BIOLOGY
AND BIOCHEMISTRY CORE FACILITIES
The Biosciences Research Precinct in the Faculty of Health Sciences of Curtin University is a
multi-user facility. The information in the form below will be used to organise training and to
make sure that the appropriate legislative requirements around health and safety, GMOs and
radiation are met. If you have any queries regarding this form please contact Rob Steuart
(contact details at bottom of form).
User Information
Name
Staff/Student
Staff/Student
Number
Department
Address
Telephone Number
Work
Mobile
Email
Principal Supervisor Details (Students Only)
Name
Staff ID
Department
Address
Telephone Number
Work
Mobile
Email
Please mark on the table below the equipment you require access to.
Room
Lab 10
Lab 7
Room Description
Post PCR,
Electrophoresis and
imaging
Equipment
Typhoon
Veriti PCR x7
Viia7
Equipment description
Use
Training
Required
Laser scanning imager
Normal endpoint PCR pseudo
gradient
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Quantitative PCR and High
resolution melt analysis.
Single tube, strips, 96w,
384w, arrays
LightCycler 480
Ion Torrent
Agarose gel
equipment
PAGE
equipment
2D PAGE
equipment
Vilber Lourmat
Imager
ChemiDoc MP
NanoDrop
Mol Prep 1
Mol Prep 2
PCR Prep
Biochemistry
DNA extraction
RNA purification and
conversion
PCR prep area. No
template DNA allowed
Purification and
functional analysis of
proteins and other
biomolecules
Misonix Probe
Sonicator
Bioruptor
sonicating
waterbath
Biospec Nano
AKTA Prime
AKTA Purifier
Biacore T200
Biacore 2000
Enspire
Dark Room
Radioisotope
suite
Envision
Lab for use of high count
radioactive material
UV
transilluminator
Rad eye monitor
Quantitative PCR and high
resolution melt analysis.
96w format only
Next generation sequencing
platform. Can do amplicon
and small genome sequencing
plus RNA seq
DNA separation
Protein separation in mini,
midi and 16cm gel formats
Two isoelectric focusing
platforms and PAGE for mini,
midi and 16cm gel formats
UV based imager
Advanced imager for
colourmetric, fluorescent,
chemiluminescent and stain
free.
Spectrophotometer for
microliter quantities.
Commonly used of nucleic
acid concentration estimation
With micro tip for 0.2-5mL
and normal tip 10-250mL
Model for next gen
sequencing library prep
Spectrophotometer for
microliter quantities.
Commonly used for nucleic
acid concentration estimation
FPLC for protein purification.
Used for proven purification
methodologies
FPLC for protein purification.
Allows for greater control of
unit for optimisation of
purification.
Surface Plasmon Resonance
for real time monitoring of
cell, protein and small
molecule interactions
As for T200. The 2000 is an
earlier version of the Biacore.
Plate reader with wide variety
of applications, not
temperature controlled
Plate reader with higher level
of control for sensitive assays.
Temperature controlled
Cutting gel bands from
Agarose gels
Radiation contamination
monitor
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Project Details
Project Title
Brief Project
Description
Max 300
words
OGTR
Do you plan to use or produce genetically modified organisms during your project?
Yes ☐
No ☐
If yes has your project been approved by the University’s Institutional Biosafety Committee?
Yes ☐
No ☐
(If no, go to this website http://healthandsafety.curtin.edu.au/hazardous_substances/biosafety.cfm)
Radiation
Do you plan to use radioisotopes during your work?
Yes ☐
No ☐
If yes which radioisotopes?
Do you or your supervisor hold a current radiation licence for the above radioisotopes?
Yes ☐
No ☐
License
Number
If using radioisotopes you will need to fill out a usage plan to document where you will be working,
what equipment you will be using and your testing regime, for both high and low count work.
If working with radioisotopes do you currently have a radiation safety badge?
Yes ☐
No ☐
Chemical Safety
Do you currently use Ethidium Bromide for staining DNA gels?
Yes ☐
No ☐
If yes please desist immediately and speak to Rob
Are you aware of the universities policy on chemical labelling?
Yes ☐
No ☐
If no please visit http://healthandsafety.curtin.edu.au/hazardous_substances/label.cfm any questions
see Rob
Are you aware of the universities policy on Risk Assessments for using chemicals?
Yes ☐
No ☐
If no please visit http://healthandsafety.curtin.edu.au/hazardous_substances/risk.cfm
User
Name
Date
Signature
Principal Supervisor (Students Only)
Name
Signature
Please return signed form to Rob Steuart
305:153
Email: r.steuart@curtin.edu.au
Phone: 9266 7362
Date