Additional Table 1. Proteins differentially expressed during skin regeneration
This table provides Gene symbol, Gene number (Entrez gene database from NCBI, http://www.ncbi.nlm.nih.gov/) and UniprotKB
(http://www.uniprot.org) of each protein, obtained from Genecards database search process (http://www.genecards.org). UniprotKB number was
used for further gene ontology enrichment analysis showed in Additional Table 2.
GENE
UniProtK GENE
Spot no.a
Protein Identityb
Protein function in fishf
symbolc
Bd
numbere
Down-regulated
90
myosin heavy polypeptide
MYH1
P12882
4619
McGuigan et al. (2004)
93
myosin heavy chain type 2 *
MYH2
Q9UKX2
4620
McGuigan et al. (2004)
175 / 286
transferrin
TF
P02787
7018
Stafford et al. (2001,2003)
204
keratin 8
KRT8
P05787
3856
Garcia et al. (2005) Zimek and Weber (2005)
207 / 427 / 448 keratin 4
KRT4
P19013
3851
Garcia et al. (2005) Zimek and Weber (2005)
214
cytokeratin 19,type I *
KRT19
P08727
3880
Zimek and Weber (2005)
235
skeletal alpha-actin type 2b
ACTB
P60709
60
Moutou et al. (2001)b
258
annexin max 3 *
ANXA3
P12429
306
Farber et al. (2006)
287
complement c1q-like protein 4
C1QL4
Q86Z23 338761
Lao et al. (2008)
288
myosin light chain 3 *
MYL3
P08590
4634
Moutou et al. (2001)a
306
dihydropyrimidinase
DPYS
Q14117
1807
Huang and Yang (2003)
348
collagen type I alpha 1
COL1A1
P02452
1277
LeGuellec et al. (2004)
392 / 396
muscle-type creatine kinase
CKM
P06732
1158
Martin-Pérez et al. (2012)
395
creatine kinase isoform a
CKM
P06732
1158
Martin-Pérez et al. (2012)
445
keratin 5
KRT5
P13647
3852
Garcia et al. (2005) Zimek and Weber (2005)
Up-regulated
210
cytokeratin 13, type I *
KRT13
P13646
3860
Zimek and Weber (2005)
239
mimecan precursor (osteoglycin)
OGN
P20774
4969
not ref
285
cofilin 2
CFL2
Q9Y281
1073
Martin-Pérez et al. (2012)
512
tributyltin binding protein type 2 *
LCN2
P80188
3934
Oba et al. (2007)
119
glyceraldehide 3P dehydrogenase
GAPDH
P04406
2597
Ibarz et al. (2010)
120
keratin 2 *
KRT2
P35908
3849
Garcia et al. (2005) Zimek and Weber (2005)
334
myosin 4 *
MYH4
Q9Y623
4622
McGuigan et al. (2004)
514
transferrin
TF
P02787
7018
Stafford et al. (2001,2003)
157 / 162
collagen type VI alpha 1
COL6A1
P12109
1291
LeGuellec et al. (2004)
516
lamin A
LMNA
P02545
4000
not ref
188
aldehyde dehydrogenase
ALDH1A1 P00352
216
Ibarz et al. (2010)
466
myo-inositol monophosphatase
IMPA1
P29218
3612
Kalujnaia and Cramb (2009)
402
immunoglobulin light chain *
IGKV
P15814
3519
not ref
515
fast skeletal myosin light chain 3
MYL3
P08590
4634
Moutou et al. (2001)a
a
Spot number from Figure 6 of the manuscript.
b
Protein identity according to zebrafish database (*indicated identified only from mammals databases).
c
Gene symbol, dGene number (Entrez gene database from NCBI, http://www.ncbi.nlm.nih.gov/) and UniprotKBd (http://www.uniprot.org) of each protein
was obtained from Genecards database search process (http://www.genecards.org).
e
UniprotKB number was used for further gene ontology enrichment analysis showed in Additional Table 2f
References referring gene determination or demonstrative protein function in fish species. Not ref: Not relevant references in fish for those proteins.
Additional References
Carrera M, Cañas B, Piñeiro C, Vázquez J, Gallardo JM (2007). De novo mass spectrometry sequencing and characterization of species-specific peptides from
nucleoside diphosphate kinase b for the classification of commercial fish species belonging to the family Merlucciidae. Journal of Proteome Research
2007, 6, 3070-3080
Farber SA, De Rose RA, Olson ES, Halpern ME (2003). The zebrafish annexin gene gamily. Genome Research 13: 1082-1096.
García DM, Bauer H, Dietz T, Schubert T, Markl J, Schaffeld M (2005). Identification of keratins and analysis of their expression in carp and goldfish:
comparison with the zebrafish and trout keratin catalog. Cell Tissue Research 322: 245–256.
Huang CY, Yang YS (2003). A novel cold-adapted imidase from fish Oreochromis niloticus that catalyzes hydrolysis of maleimide. Biochemical and
Biophysical Research Communications 312: 467–472.
Kalujnaia S, Cramb G. (2009). Regulation of expression of the myo-inositol monophosphatase 1 gene in osmoregulatory tissues of the European eel Anguilla
anguilla after seawater acclimation. Trends in Comparative Endocrinology and Neurobiology: Ann. N.Y. Acad. Sci. 1163: 433–436.
McGuigan K, Phillips,_PC, Postlethwait JH (2004). Evolution of sarcomeric myosin heavy chain genes: evidence from fish. Molecular Bioliology and
Evolution. 21(6):1042–1056.
Moutou KA, Canario AVM , Mamuris Z, Power DM (2001). Molecular cloning and sequence of Sparus aurata skeletal myosin light chains expressed in
white muscle: developmental expression and thyroid regulation. The Journal of Experimental Biology 204, 3009–3018
Moutou KA, Socorro S, Power DM, Mamuris Z, Canario AVM (2001). Molecular cloning and sequence of gilthead sea bream (Sparus aurata) -skeletal
actin: tissue and developmental expression. Comparative Biochemistry and Physiology Part B 130, 13-21
Santos CR, Power DM, Kille P, Llewellyn L, Ramsurn V, Wigham T, Sweeney GE. (1997). Cloning and sequencing of a full-length sea bream (Sparus
aurata) beta-actin cDNA. Comparative Biochemestry and Physiology B 117(2):185-9.
Zimek A, Weber K (2005). Terrestrial vertebrates have two keratin gene clusters; striking differences in teleost fish. European Journal of Cell Biology 84,
623–635