Supplementary material
An efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain obtained
through adaptive evolution and its global transcription profile
Yu Shen¶, Xiao Chen¶, Bingyin Peng, Liyuan Chen, Jin Hou, and Xiaoming Bao*
The State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China
¶
Shared first author
*Corresponding author. Mailing address: The State Key Laboratory of Microbial Technology,
Shandong University, Shan Da Nan Road 27#, Jinan, 250100, China. Phone and Fax: +86 531
88365826. E-mail Address: bxm@sdu.edu.cn.
16
Doubling time (hour)
14
12
10
8
6
4
2
0
0
100
200
300
400
500
600
700
Adaptive evolution time (hour)
Fig. S1 The adaptive evolution of strains in xylose. The BSPC095 (Pi-XI, XK, gre3::PPP,
cox4) was cultured at 30 °C and sequentially transferred into fresh xylose minimal medium
during the early stationary phase.
Fig. S2 The morphologic identification and Calcofluor white staining of the strains. The cells
were harvested during the exponential phase. Morphologic identification of the (A) BSPC016
and (B) BSPC016/cwp1 strains. The (C) BSPC016 and (D) BSPC016/cwp1 cells were
treated with 5 g mL−1 Calcofluor white for 10 min.
Fig. S3 Cultivation characteristics of the strains on 4 g l-1 xylose. Symbols: ■, BSPC016; ▲,
BSPC016
Table S1. Oligonucleotides used in this study
Oligonucleotides
PiXylAs
PiXylAa
p6KanFor
p6KanRev
INphoKanFor
INKanphoRev
cwp1For
cwp1Rev
pho84For
pho84Rev
Ypho13-For
Ypho13-Rev
Ypho84-For
Ypho84-Rev
Ycwp1-For
Ycwp1-Rev
PFK27BglFor
PFK27BglRev
PDC6BamHFor
PDC6BamHRev
OPT2BglFor
OPT2BglRev
YLRBglFor
YLRBglRev
RTACT-F
RTACT-R
RTPFK27-F
RTPFK27-R
RTPDC6-F
RTPDC6-R
RTOPT2-F
Sequence (5’-3’)
TTTggatccACAATGGCTAAGGAATATTTCCCACAAA
ATCctgcagTTTATTGGTACATGGCAACAATAGCTTC
CAGCTGAAGCTTCGTACGCTG
GCATAGGCCACTAGTGGATCTG
GATGTGACATCTTTACTATTCTCCAGCACGTTTTCAGCAGCTGAAGCTTCGTACGCTG
TTCTTCACCAATACCGCTTTCTCCAAATACCCATACGCATAGGCCACTAGTGGATCTG
TATTCGCCTTGGCTAAGATGGTCATTGCCGATTCCGAAGAACAGCTGAAGCTTCGTACG
CTGTTTGTTCGTAAACTGTGTTTGGAGCTTGGATTTGTCCAGCATAGGCCACTAGTGGA
TCCTCATCTCTTGTATAAAGTAAAGTTCTAAGTTCACTTCTCAGCTGAAGCTTCGTACG
TATGGTCGATTAGAGTACCCAAAGCGGTTTGTGCAATAATGGCATAGGCCACTAGTGGA
GATGTGACATCTTTACTATTCTCC
CAATTCTTCACCAATACCGCTTTC
CCTCATCTCTTGTATAAAGTAAAGTTC
AGAGCGATGATACCACCGGAG
TATTCGCCTTGGCTAAGATGGTC
CAACAAGTAAGCAGCTGCGAC
GATCagatctATGGGTGGTTCTTCCGA
CATGagatcTTCAAGCAAATCCGTTGCT
GATCggatccATGTCTGAAATTACTCTTG
GTACggatccTGGCTTATTGTTTGGCAT
GATCagatctATGAGTGAAACAGTCAAAG
CATGagatctTCATGGATAGTGTCCTGG
GATCagatctATGATATTTGCTCCTTCT
CATGagatctCACTTAAAGGGCAACTGC
ATGCAAACCGCTGCTCAA
AGTTTGGTCAATACCGGCAGA
CGACCCGTTACACTTACCCATTAC
CACAACTGATTTGCCTTCTTATCTTACC
GGTATCTCGCAGGTGTTGTGG
ATAGTGTAGCCGTCGTTGTTAAGG
TTGCCATATTGATAGGTTCACTTGTTG
Purpose
Amplifying the Pi-xylA gene
Amplifying the DNA fragment
loxP-kanMX-loxP
Amplifying the DNA fragment for the PHO13
gene deletion
Amplifying the DNA fragment for the CWP1
gene deletion
Amplifying the DNA fragment for the PHO84
gene deletion
For gene pho13 certification
For gene pho84 certification
For gene cwp1 certification
Amplifying the PFK27 gene
Amplifying the PDC6 gene
Amplifying the OPT2 gene
Amplifying the YLR040C gene
The qPCR primer for ACT1
The qPCR primer for PFK27
The qPCR primer for PDC6
The qPCR primer for OPT2
RTOPT2-R
RTYLR040C-F
RTYLR040C-R
XI-F
XI-R
Actin-F
Actin-R
GTTGCTTGAAGGATAGTAGTTGGAATC
CCTCCGCCACCACCATATACC
CGAGAAAGGAACAGAATTGGAAACG
ACCACCAGTAACGAAACCAC
GTGCTGTTGATGCTGGTATG
CAAACCGCTGCTCAATCTTC
AGTTTGGTCAATACCGGCAG
The qPCR primer for YLR040C
Plasmid copy number determination
Table S2. The ratio of gene transcriptional level for the adapted strain BSPX013 (Pi-XI, XK, gre3::PPP, cox4, AE) versus the parental strain
BSPC095 (Pi-XI, XK, gre3::PPP, cox4)
Systematic name
Standard name
Fold-change
Description
Glycolysis, TCA circle,pentose phosphate pathway, glucose transporter
YOL136C
PFK27
1.69
6-phosphofructo-2-kinase 2
YGR087C
PDC6
1.75
Pyruvate decarboxylase isozyme 3
YLR174W
IDP2
1.75
Isocitrate dehydrogenase
YFR053C
HXK1
0.47
Hexokinase-1
YDR516C
EMI2
0.65
Glucokinase EMI2
YMR105C
PGM2
0.34
Phosphoglucomutase-2
YGR248W
SOL4
0.4
Probable 6-phosphogluconolactonase 4
YDL022W
GPD1
0.46
Glycerol-3-phosphate dehydrogenase [NAD+] 1
YER062C
HOR2
0.53
DL-glycerol-3-phosphatases
YDL194W
SNF3
0.57
High-affinity glucose transporter
YDR342C
HXT7
0.62
High-affinity hexose transporter
YDR343C
HXT6
0.64
High-affinity hexose transporter
YHR092C
HXT4
0.64
Low-affinity glucose transporter
YHR094C
HXT1
0.46
Low-affinity glucose transporter
YJL219W
HXT9
0.57
Hexose transporter
YJR158W
HXT16
0.56
Hexose transporter
YMR011W
HXT2
0.65
High-affinity glucose transporter
YOL156W
HXT11
0.5
Hexose transporter
Nitrogen and amino acid metabolism
YIR027C
DAL1
2.4
Allantoinase
YIR029W
DAL2
2.91
Allantoicase
YIR032C
DAL3
2.02
Ureidoglycolate hydrolase
YJR152W
DAL5
7.73
Allantoate permease
YIR031C
DAL7
3.28
Malate synthase 2, glyoxysomal
YBR208C
DUR1,2
2.7
Urea amidolyase
YLR155C
ASP3-2
6.37
L-asparaginase 2 precursor
YLR157C
ASP3-2
6.84
L-asparaginase 2 precursor
YLR158C
ASP3-2
6.22
L-asparaginase 2 precursor
YLR160C
ASP3-2
5.46
L-asparaginase 2 precursor
YLR142W
PUT1
5.63
Proline oxidase, mitochondrial precursor
YOR348C
PUT4
2.99
Proline-specific permease
YFR055W
IRC7
2.65
Putative cystathionine beta-lyase
YKR039W
GAP1
2.71
General amino-acid permease
YDL215C
GDH2
12.48
NAD-specific glutamate dehydrogenase
YPR035W
GLN1
1.89
Glutamine synthetase
YFL030W
AGX1
2.21
Alanine--glyoxylate aminotransferase 1
YHR137W
ARO9
1.95
Aromatic amino acid aminotransferase 2
YDR428C
ARO10
2.74
Transaminated amino acid decarboxylase
YJR148W
BAT2
2.56
Branched-chain-amino-acid aminotransferase, cytosolic
YEL046C
GLY1
1.54
Low specificity L-threonine aldolase
YDR428C
BNA7
1.52
Serine hydrolase
YLL055W
YCT1
1.51
High-affinity cysteine-specific transporter
YNL142W
MEP2
4.08
Ammonium transporter
YGR121C
MEP1
1.69
Ammonium transporter
YKL218C
SRY1
1.83
Threo-3-hydroxyaspartate ammonia-lyase
YMR088C
VBA1
2.95
Vacuolar basic amino acid transporter 1
YMR250W
GAD1
0.5
Glutamate decarboxylase
YPL111W
CAR1
0.56
Arginase
YDR046C
BAP3
0.3
Valine amino-acid permease
YDR508C
GNP1
0.19
High-affinity glutamine permease
YCL025C
AGP1
0.21
General amino acid permease
YBR132C
AGP2
0.55
General amino acid permease
YOL020W
TAT2
0.64
Tryptophan permease
YPR138C
MEP3
0.56
Ammonium transporter
Stress response element genes
YDR256C
CTA1
0.38
Peroxisomal catalase A
YGR088W
CTT1
0.16
Cytosolic catalase T
YML100W
TSL1
0.38
Trehalose synthase complex regulatory subunit TSL1
YEL011W
GLC3
0.46
1,4-alpha-glucan-branching enzyme
YFR015C
GSY1
0.17
Glycogen [starch] synthase isoform 1
YDR074W
TPS2
0.52
Trehalose-phosphatase
YMR261C
TPS3
0.53
Trehalose synthase complex regulatory subunit TPS3
YPR160W
GPH1
0.6
Glycogen phosphorylase
YLR258W
GSY2
0.63
Glycogen [starch] synthase isoform 2
YKR058W
GLG1
0.46
Glycogen synthesis initiator protein
YIL045W
PIG2
0.54
GSY2-interacting protein
Hot shock element genes
YBR072W
HSP26
3.15
Heat shock protein 26
YDR171W
HSP42
0.46
Heat shock protein 42
YLL026W
HSP104
0.63
Heat shock protein 104
YJL034W
KAR2
0.61
ATPase involved in protein import into the ER
YCR021C
HSP30
0.34
30 kDa heat shock protein.
YKL073W
LHS1
0.67
Heat shock protein 70 homolog LHS1 precursor
YJL153C
INO1
5.6
Inositol-3-phosphate synthase
YOL103W
ITR2
1.52
Myo-inositol transporter
YMR095C
SNO1
2.18
Probable glutamine amidotransferase
YNL334C
SNO2
1.52
Probable glutamine amidotransferase
YMR096W
SNZ1
1.68
Pyridoxine biosynthesis protein
YOL143C
RIB4
0.57
6,7-dimethyl-8-ribityllumazine synthase
Vitamin metabolism
Iron and copper metabolism
YOL152W
FRE7
8.18
Ferric reductase transmembrane component 7
YLR214W
FRE1
3.76
Ferric reductase transmembrane component 1 precursor
YHR055C
CUP1-1
2.15
Metallothionein precursor
YHR053C
CUP1-1
2.25
Metallothionein precursor
YHL040C
ARN1
1.94
Siderophore iron transporter ARN1
YMR058W
FET3
2.37
Iron transport multicopper oxidase FET3 precursor
YDR270W
CCC2
1.5
Copper-transporting ATPase
YER145C
FTR1
1.78
Plasma membrane iron permease
YPL135W
ISU1
1.85
Iron sulfur cluster assembly protein 1, mitochondrial precursor
Mating and sporulation
YIL037C
PRM2
4.93
Pheromone-regulated membrane protein 3
YGR032W
GSC2
4.67
1,3-beta-glucan synthase component
YLR307W
CDA1
2.75
Chitin deacetylase 1 precursor
YPL130W
SPO19
2.41
Sporulation-specific protein 19 precursor
YIL015W
BAR1
2.37
Aspartyl protease
YHR184W
SSP1
2.27
Sporulation-specific protein 1
YIL117C
PRM5
2.25
Pheromone-regulated protein
YLR213C
CRR1
2.03
Probable glycosidase CRR1 precursor
YLR054C
OSW2
1.88
Outer spore wall protein 2
YEL072W
RMD6
1.87
Sporulation protein
YDR461W
MFA1
1.86
Mating hormone A-factor 1 precursor
YOR313C
SPS4
1.77
Sporulation-specific protein 4
YIL073C
SPO22
1.77
Sporulation-specific protein 22
YIL099W
SGA1
1.72
Glucoamylase
YPL156C
PRM4
1.71
Pheromone-regulated membrane protein 4
YGR032W
AGA2
1.62
A-agglutinin-binding subunit precursor
YMR306W
FKS3
1.61
1,3-beta-glucan synthase component
YKL178C
STE3
1.61
Pheromone a factor receptor
YOR177C
MPC54
1.51
Meiotic plaque component protein 54
YJL108C
PRM10
0.5
Pheromone-regulated membrane protein 10.
YPR002W
PDH1
1.54
2-methylcitrate dehydratase
YEL024W
RIP1
1.61
Ubiquinol-cytochrome c reductase iron-sulfur subunit, mitochondrial precursor
YOR065W
CYT1
1.6
Cytochrome c1, heme protein, mitochondrial precursor
Respiration
Q0250
COX2
1.51
Cytochrome c oxidase subunit 2 precursor
YNL052W
COX5A
1.59
Cytochrome c oxidase polypeptide Va, mitochondrial precursor
YMR256C
COX7
1.54
Cytochrome c oxidase subunit 7
YBR039W
ATP3
1.54
ATP synthase gamma chain, mitochondrial precursor
YDR298C
ATP5
1.53
ATP synthase subunit 5, mitochondrial precursor
YDL181W
INH1
1.55
ATPase inhibitor, mitochondrial precursor
Q0105
COB
0.3
Cytochrome b
Q0045
COX1
0.46
Cytochrome c oxidase subunit 1
YML054C
CYB2
0.61
Cytochrome b2, mitochondrial precursor
Q0120
BI4
0.63
Intron-encoded RNA maturase bI4 precursor
Q0050
AI1
0.62
Putative COX1/OXI3 intron 1 protein.
YEL039C
CYC7
0.38
Cytochrome c iso-2.
YLR056W
ERG3
1.82
C-5 sterol desaturase
YLR133W
CKI1
1.99
Choline kinase
YKR053C
YSR3
1.87
Dihydrosphingosine 1-phosphate phosphatase
YJR019C
TES1
1.56
Peroxisomal acyl-coenzyme A thioester hydrolase 1
YMR101C
SRT1
2.04
Putative dehydrodolichyl diphosphate synthetase
YOR317W
FAA1
0.58
Long-chain-fatty-acid--CoA ligase 1
YKR034W
DAL80
9.99
Nitrogen regulatory protein
YPL054W
LEE1
2.4
Zinc finger protein
YBR182C
SMP1
2.13
Transcription factor
YLR136C
TIS11
2.33
mRNA-binding zinc finger protein
Lipid Metabolism
Transcription factor
YNL093W
YPT53
2.16
GTP-binding protein
YGL121C
GPG1
2.43
Heterotrimeric G protein gamma subunit
YOR315W
SFG1
2.34
Superficial pseudohyphal growth protein 1
YKL109W
HAP4
1.83
Transcriptional activator
YPR065W
ROX1
1.94
Repressor
YIL101C
XBP1
1.94
Transcriptional repressor
YDR043C
NRG1
1.65
Transcriptional regulator
YEL009C
GCN4
1.53
General control protein
YFL031W
HAC1
1.64
Transcriptional activator
YPL202C
AFT2
1.54
Iron-regulated transcriptional activator
YBL049W
MOH1
1.51
Protein yippee-like
YKL043W
PHD1
1.55
Putative transcription factor
YBR033W
EDS1
1.97
Uncharacterized transcriptional regulatory protein
YDR309C
GIC2
1.59
GTPase-interacting component 2
YPL141C
YPL141C
1.72
Putative serine/threonine-protein kinase
YGR161C
RTS3
1.81
Putative component of the protein phosphatase type 2A complex
YMR199W
CLN1
1.5
G1/S-specific cyclin
YCL027W
FUS1
1.63
Nuclear fusion protein
YBL016W
FUS3
1.98
Mitogen-activated protein kinase
YGR040W
KSS1
1.67
Mitogen-activated protein kinase
YHR124W
NDT80
1.64
Meiosis-specific transcription factor
YDR277C
MTH1
0.41
Negative regulator
YGR249W
MGA1
0.37
Protein similar to heat shock transcription factor
YGR052W
FMP48
0.39
Probable serine/threonine-protein kinase
YJL141C
YAK1
0.51
Dual specificity protein kinase
YMR104C
YPK2
0.54
Serine/threonine-protein kinase
YOL016C
CMK2
0.57
Calcium/calmodulin-dependent protein kinase II
YJR059W
PTK2
0.6
Serine/threonine-protein kinase
YPR106W
ISR1
0.66
Serine/threonine-protein kinase
YMR291W
YMR291W
0.65
Probable serine/threonine-protein kinase
YDL079C
MRK1
0.66
Serine/threonine-protein kinase
YJL106W
IME2
0.59
Meiosis induction protein kinase
YNR031C
SSK2
0.66
MAP kinase kinase kinase
YAR003W
SWD1
0.77
COMPASS component SWD1
YGR097W
ASK10
0.64
Component of RNA polymerase II holoenzyme
YPR194C
OPT2
11.87
Oligopeptide transporter 2
YJR078W
BNA2
2.21
Indoleamine 2,3-dioxygenase family protein
YJL172W
CPS1
2.08
Vacuolar carboxypeptidase S
YKR061W
KTR2
2.26
Probable mannosyltransferase
YLR121C
YPS3
1.85
Aspartic proteinase yapsin-3 precursor
YKL103C
LAP4
1.63
Vacuolar aminopeptidase 1 precursor
YNL015W
PBI2
1.63
Cytosolic inhibitor of vacuolar proteinase B
YLR297W
YLR297W
1.72
Uncharacterized vacuolar protein
YLR099C
ICT1
1.57
Putative serine protease
YPL058C
PDR12
1.5
ATP-dependent permease
YGR142W
BTN2
0.34
v-SNARE binding protein
YPL019C
VTC3
0.61
Vacuolar transporter chaperone 3
YNL153C
GIM3
0.62
Prefoldin subunit 4
YMR094W
CTF13
2.2
Centromere DNA-binding protein complex CBF3 subunit C
YER171W
RAD3
3.08
DNA repair helicase
others
YPL127C
HHO1
1.57
Histone H1
YIL123W
SIM1
1.58
Protein SIM1 precursor
YFR038W
IRC5
0.6
Uncharacterized ATP-dependent helicase
YJL168C
SET2
0.66
Histone-lysine N-methyltransferase, H3 lysine-36 specific
YML128C
MSC1
0.62
Meiotic sister chromatid recombination protein 1
YJR077C
MIR1
1.5
Mitochondrial phosphate carrier protein
YDR281C
PHM6
1.56
Phosphate metabolism protein 6
YML123C
PHO84
0.27
Inorganic phosphate transporter
YOL084W
PHM7
0.54
Phosphate metabolism protein 7
YGR189C
CRH1
2.06
Probable glycosidase CRH1 precursor
YBR299W
MAL32
0.36
Alpha-glucosidase
YGR292W
MAL12
0.45
Alpha-glucosidase
YBR298C
MAL31
0.33
Maltose permease
YGR289C
MAL11
0.22
General alpha-glucoside permease
YGR287C
YGR287C
0.53
Probable alpha-glucosidase
YEL040W
UTR2
0.64
Probable glycosidase CRH2 precursor
YMR305C
SCW10
1.85
Probable family 17 glucosidase SCW10 precursor
YKL217W
JEN1
1.69
Carboxylic acid transporter protein homolog
YGR260W
TNA1
1.54
High-affinity nicotinic acid transporter
YBR287W
ZSP1
1.56
Uncharacterized transporter
YKL221W
MCH2
1.72
Probable transporter
YPL088W
YPL088W
1.6
Putative aryl-alcohol dehydrogenase
YML087C
YML087C
1.79
Uncharacterized oxidoreductase
YIR030C
DCG1
2.01
Protein
YDR368W
YPR1
0.48
Putative reductase 1
YDL246C
SOR2
0.66
Sorbitol dehydrogenase 2
YDR368W
YPR1
0.48
NADPH-dependent aldo-keto reductase
YML130C
ERO1
0.67
Endoplasmic oxidoreductin-1 precursor
YOL151W
GRE2
0.47
3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase GRE2
YKL216W
URA1
0.65
Dihydroorotate dehydrogenase
YKR076W
ECM4
1.6
Glutathione transferase omega-like 2
YER175C
TMT1
1.68
Trans-aconitate methyltransferase, Cytosolic enzyme
YOL164W
BDS1
1.53
Alkyl/aryl-sulfatase
YEL066W
HPA3
1.53
N-acetyltransferase
YOL076W
MDM20
0.67
N-terminal acetyltransferase B complex subunit
YMR085W
YMR085W
0.54
Putative glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
YPR105C
COG4
0.58
Conserved oligomeric Golgi complex subunit 4
YEL060C
PRB1
1.72
Cerevisin precursor
YBR233WA
N/A
0.35
RNA binding protein
YHR087W
YHR087W
0.32
Protein of unknown function involved in RNA metabolism
YBR294W
SUL1
1.86
Sulfate permease 1
YNL237W
YTP1
2.1
Probable type-III integral membrane protein of unknown function
YBL043W
ECM13
2.86
YLR042C
YLR042C
2.63
Cell wall protein YLR042C precursor
YLR194C
YLR194C
2.09
Cell wall protein YLR194C precursor
YDR034W
N/A
2.16
Predicted tail-anchored plasma membrane protein
YBR162C
TOS1
1.8
Covalently-bound cell wall protein of unknown function
YFL061W
DDI3
1.81
Protein of unknown function
YKR091W
SRL3
1.63
Cytoplasmic protein
YKR013W
PRY2
1.85
Protein of unknown function
YDR262W
YDR262W
1.55
FAS1 domain-containing protein YDR262W precursor
YGR239C
PEX21
1.78
Peroxisomal membrane protein
YKL071W
YKL071W
1.49
Uncharacterized protein
YOR382W
FIT2
1.81
Facilitator of iron transport 2 precursor
YNR002C
ATO2
1.73
Putative transmembrane protein
YOL019W
YOL019W
1.58
Uncharacterized membrane protein
YLR414C
YLR414C
1.56
Cell membrane protein YLR414C precursor
YBL069W
AST1
1.51
Peripheral membrane protein
YPR075C
OPY2
1.51
Integral membrane protein
YNL036W
NCE103
1.71
Non-classical export protein 3
YIL121W
QDR2
1.68
Uncharacterized transporter
YDR055W
PST1
1.79
Protoplast secreted protein 1 precursor
YKL096W
CWP1
0.15
Cell wall protein CWP1 precursor.
YDR077W
SED1
0.49
Cell wall protein SED1 precursor.
YDL049C
KNH1
0.57
Cell wall synthesis protein KNH1 precursor.
YML116W
ATR1
0.62
Aminotriazole resistance protein.
YOR161C
PNS1
0.26
Postulated to be a choline transporter
YLR413W
YLR413W
0.34
Cell membrane protein YLR413W precursor.
YDL206W
YDL206W
0.55
Putative cation exchanger YDL206W precursor.
YBR067C
TIP1
1.78
Temperature shock-inducible protein 1 precursor
YER185W
YER185W
1.82
Uncharacterized protein
YFL010C
WWM1
1.54
WW domain-containing protein
YGR153W
YGR153W
1.55
Uncharacterized protein
YNL335W
DDI3
1.65
Uncharacterized protein
YDR379C-A
N/A
1.49
N/A
YIR042C
YIR042C
1.86
Uncharacterized protein
YEL073C
YEL073C
1.57
Uncharacterized protein
YBR266C
N/A
1.5
N/A
YJR079W
YJR079W
1.74
Uncharacterized protein
YBR071W
YBR071W
1.5
Uncharacterized protein
YHR138C
YHR138C
1.71
Uncharacterized protein YHR138C precursor
YMR107W
SPG4
2.13
Uncharacterized protein
YPL163C
SVS1
1.8
Protein SVS1 precursor
YNL208W
YNL208W
1.65
Uncharacterized protein
YOL163W
YOL163W
1.76
Putative protein of unknown function
YJL037W
IRC18
2.11
Uncharacterized protein
YLR407W
YLR407W
1.53
Uncharacterized protein
YHR029C
YHI9
1.84
Uncharacterized isomerase
YFR017C
YFR017C
0.59
Uncharacterized protein
YLR177W
YLR177W
0.61
Uncharacterized protein
YOL014W
YOL014W
0.45
Putative uncharacterized protein
YBR054W
YRO2
0.63
Putative protein of unknown function
YGR243W
FMP43
0.5
Putative protein of unknown function
YJL070C
YJL070C
0.66
Uncharacterized deaminase
YNL108C
YNL108C
0.67
Uncharacterized protein
YLR460C
YLR460C
0.67
Uncharacterized protein
YCL027CA
N/A
0.58
N/A
YML131W
YML131W
0.38
Uncharacterized membrane protein
YFL051C
YFL051C
0.49
Uncharacterized membrane protein YFL051C precursor.
YKR075C
YKR075C
0.52
Uncharacterized protein YKR075C.
YMR084W
YMR084W
0.51
Putative protein of unknown function
YER067W
YER067W
0.35
Uncharacterized protein
YDR367W
YDR367W
0.52
Uncharacterized membrane protein
YHL021C
FMP12
0.66
Uncharacterized oxidoreductase
YDL241W
YDL241W
0.63
Uncharacterized protein
YGR131W
YGR131W
0.65
Uncharacterized membrane protein
YPL014W
YPL014W
0.58
Uncharacterized protein
Table S3. The glucose-xylose cofermentation of BSPC112 (Pi-XI, XK) series strains under oxygen-limited conditions
Strains
BSPC112
Consumed Consumed Product yield (g (g consumed
glucose
xylose
sugars) -1)
(g l-1)
(g l-1)
Biomassb Xylitolc Ethanold
19.32
3.12
0.086
0.531
0.363
Specific consumption or production ratea (mmol (g
biomass) -1 h-1)
Xylose
Xylitol
Glycerol Acetate Ethanol
0.132
0.079
0.001
-0.033
0.017
Carbon
recovery
0.90
BSPC112/pho13 19.20
4.67
0.092
0.686
0.301
0.207
0.173
0.002
0.312
-0.238
0.97
BSPC112/cwp1
19.21
3.42
0.112
0.872
0.280
0.129
0.137
0.001
0.278
-0.312
0.96
BSPC112/PFK27
19.21
4.69
0.095
0.618
0.300
0.191
0.146
0.001
0.267
-0.241
0.94
BSPC112/PDC6
19.23
4.53
0.093
0.627
0.312
0.184
0.142
0.001
0.241
-0.207
0.95
Strains were cultured in the shake flasks for 72 h using 20 g l-1 glucose and 20 g l-1 xylose as carbon source. A rubber stopper was used to maintain
the oxygen-limited condition. The values are given as the average and experimental deviation of two independent experiments.
a
Specific consumption or production rates of metabolites were the data of xylose-consuming phase after glucose depletion.
b
Biomass yield was calculated based on glucose only.
c
Xylitol yield was calculated based on consumed xylose only.
d
Ethanol yield was calculated based on consumed glucose and xylose.