Supplementary Fig S1. Genetic linkage analysis of the hygromycin (hyg) insertion and highCO2 (HC)-requiring phenotype. Genomic DNA was extracted from tetrad progeny (A-L) from
crosses of H82 and CC-1690. Genomic PCR was performed with the primer sets (F1 and UP-S
or R1 and DP-S) depicted in Fig. 4B. For spot test, progenies grown to logarithmic phase were
diluted to the indicated optical density (OD430 0.15, 0.07, or 0.03), 3 µL of each suspension was
spotted onto HSM plates, and incubated in a growth chamber supplied with LC for 4 days.
Supplementary Fig S2. Genetic linkage analysis of the hygromycin (hyg) insertion and highCO2 (HC)-requiring phenotype. Genomic DNA was extracted from tetrad progeny (M-X) from
crosses of H82 and CC-125. Genomic PCR was performed with the primer set (DP-S and R2)
depicted in Fig. 4B. For spot test, progenies grown to logarithmic phase were diluted to the
OD430 of 0.07, 3 µL of each suspension was spotted onto HSM plates, and incubated in a growth
chamber supplied with HC or LC for 6 days. For hyg resistance test, progenies were directly
streaked onto TAP plates containing 30 µg mL-1 hyg, and incubated under HC condition for 6
days.