SUPPLEMENTARY DATA
Reconciliation of MATX gene tree with species tree
We have used the software Jane (http://www.cs.hmc.edu/~hadas/jane/) to reconcile the MATX gene tree with
the species tree. For this analysis we have excluded taxa with very incomplete sequence (Prymnesium) or taxa,
whose MATX sequences could be result of contamination (Lactuca and Dencroctonus). If we set the cost of
gene loss to 0, which could be a realistic value in case of loss of one of two paralogues, then the discrepancy
between MATX gene tree and species tree can be explained by the same number of events if we consider
duplications and differential losses (A) or horizontal gene transfers (B).
A
B
Although we cannot objectively reject alternative (A), we consider it less likely. Note that this alternative
expects that the ancestors of dinoflagellates and stramenopiles would harbour four paralogues of the enzyme
(three paralogues of MATX and one paralogue of MAT) over a long period of evolutionary time (marked by
curly brace). This is unrealistic in our opinion because most extant species contain only one paralogue.
MAT and MATX genes (translated proteins) of euglenids assembled from transcriptome projects.
>Euglena clara methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTCCTTTTCTCCTCTGAGTCAGTCAACGAGGGTCATCCCGACAAGCTCTGTGATCAGGTCTCTGATTCTGTGCTGGACGCATGTTTGACTGAGGACCCC
AAGAGCAAGGTGGCCTGTGAGACAGCTGTCAAGGACAACATGGTTATGGTTCTGGGCGAGATCACCACCCAAGCTAAGCTGGATTATGACGCCATTGTGC
GCAAGGCCGTTCGAAACATTGGCTTTGACTCCTTCGTAGATGACTTGTCCAGCGTGGATTCCAAGGGCCTGAGCTGTGACGACTGTGAGGTGTTGGTGCGA
ATCAACAAGCAATCCCCAGACATTGCTGGCGGTGTGCACGTTGGGCGTGATGAGATGGATTTGGGAGCTGGTGATCAGGGCATTATGTTTGGATATGCCT
CTGATGAGACCCAGAGCACCATGCCCCTTACCCATTACCTCGCCACTAGGCTTGGCAAGACCCTCACCGACGTCCGCAAAGATGGCTCCCTCTGGTGGCTCC
GTCCCGACGGCAAGACCCAGGTGACCATCAAGTACAAGCAGTGCCCCGATGGCTCTGTGGAGCCCCTGCTGATTCACACAGTGTTGATCTCCACGCAGCAC
TGCGAGCCCGGGAAGCGCACGAAGGGGGAGGAGATCAAGGGATATACCGGCCCGGATGCCGATCTGGTGGCCCCTTCCATGGAGGAGATGAATGAGCT
GATTATTCAGCATGTCGTGATCCGCACCCTGCAGGACATCACCTTGAAGAACGGGCAGCCTGCTTTGAGCATCTTCGACCGGAATACCTGCCATCTGCACAT
GAACCCCTCAGGGAAATTTATTATTGGAGGCCCGCAGGGGGATGCTGGCCTGACTGGCCGCAAGATCATCATTGATACATACGGTGGCTGGGGCGCCCAC
GGTGGTGGTGCTTTCTCCGGCAAGGACCCCACCAAGGTGGACCGTTCCGCGGCGTACATCACCCGCCAAATCGCCAAGTCCATCGTGGTCTCCAAGTTGGC
AAAGCGAGCATTGGTCCAGTTGTCGTACGCCATTGGCGTTGCCAAGCCCCTGTCCATCTTCGTGGAGACATATGGCTCGGAGCAGGGAGCCCTCTCGGCCG
ATGACATCACGGACATTGTGAAGCTCGCATTTGACTGCCGTCCCGGTGCCATCGCCAAGTCCCTGCAGCTGAGGGAGCCCAAGTATGTCGAGACAGCAGC
CTATTGCCATTTTGGGCGGGAGCCCCGCACGGAGAATGGTATCAAGTATTTTGAGTGGGAGAATCCGGTAGACCTGTCCAAG
> Euglena clara methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLTEDPKSKVACETAVKDNMVMVLGEITTQAKLDYDAIVRKAVRNIGFDSFVDDLSSVDSKGLSCDDCEVLVRINKQS
PDIAGGVHVGRDEMDLGAGDQGIMFGYASDETQSTMPLTHYLATRLGKTLTDVRKDGSLWWLRPDGKTQVTIKYKQCPDGSVEPLLIHTVLISTQHCEPGKRTK
GEEIKGYTGPDADLVAPSMEEMNELIIQHVVIRTLQDITLKNGQPALSIFDRNTCHLHMNPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQIAKSIVVSKLAKRALVQLSYAIGVAKPLSIFVETYGSEQGALSADDITDIVKLAFDCRPGAIAKSLQLREPKYVETAAYCHFGREPRTENGIKYFEWENP
VDLSK
>Euglena viridis methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTTCTCTTCTCTTCAGAATCTGTGAATGAGGGCCATCCCGATAAGCTGTGCGACCAGGTCTCTGACTCTGTGCTGGATGCCTGCCTTGCCCAAGATCCC
AAGAGTAAGGTCGCGTGTGAGACTGCAGTGAAGGACAACATGGTCATGGTTCTGGGTGAGATCACCACCCAAGCTAAATTGGACTATGAGGCCATTGTCC
GCAAGGCTGTGCGGAACATCGGATTTGATTCCTTTGTAGACGATCTTTCGAGTGTGGATTCTAAGGGACTGAACTGCGATGACTGTGAAGTACTGGTCCGC
ATCAACAAGCAGTCCCCCGACATTGCCGGTGGAGTTCACGTTGGTCGTGATGAGCTGGACTTTGGGGCAGGAGATCAGGGCATTATGTTTGGCTATGCAA
CTGATGAGACCAAGAGCACCATGCCCCTCACCCACTACCTAGCTACCCGCTTGGGTAAGACATTGACTGATGTCCGCAAAGATGGCACCCTTTGGTGGCTG
CGCCCTGATGGTAAGACACAGGTGACAATCAAATATAAGCAATGTGCCGATGGGTCTGTGGAACCACTGTTGATCCACACTGTTTTGATCTCTACCCAGCA
TTGTGAACCTTCCAAGCGAACCAAGGGAGAGGAGATCAAGGGATACAAGGGGGAAGATGCTGGAGAAGTGGCTCCTTCCATGGACCAGATGAATGAGCT
CATCATCCAGCATGTGGTTATCCGTACCCTGCAGGACATCACGTTGAAGAACGGACAGCCTGCTCTGAGTATCTTTGACCGTAATACCTGCCATCTTCACAT
CAACCCCTCCGGCAAGTTCATTATTGGAGGACCTCAGGGTGATGCTGGTCTGACAGGCCGAAAGATTATCATTGACACCTATGGAGGGTGGGGTGCTCAT
GGAGGTGGTGCTTTCTCTGGAAAGGATCCCACCAAGGTTGATCGATCTGCAGCCTACACCACCCGTCAGATTGCCAAATCTATTGTTGTCTCCAAGCTGGCC
AAACGTGCTCTTGTCCAGCTGTCCTATGCCATTGGCGTGGCCAAGCCTCTGTCCATCTTTGTGGAAACCTATGGCTCCGAACAGGGAGCATTGACAGCGGA
TGACATCACAGACATTGTCAAGCTTGCCTTTGACTGCCGACCAGGTGCCATTGCCAAGTCCCTCCAGCTGACAGAGCCCAAGTATGTGGAGTCTGCTGCCTA
CTGCCATTTTGGCCGGGAGCCCCGCACCGAGAATGGCATCAAGTTCTTCGAGTGGGAGAACCCTGTCGACCTCTCCTCT
>Euglena viridis methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAQDPKSKVACETAVKDNMVMVLGEITTQAKLDYEAIVRKAVRNIGFDSFVDDLSSVDSKGLNCDDCEVLVRINKQ
SPDIAGGVHVGRDELDFGAGDQGIMFGYATDETKSTMPLTHYLATRLGKTLTDVRKDGTLWWLRPDGKTQVTIKYKQCADGSVEPLLIHTVLISTQHCEPSKRTK
GEEIKGYKGEDAGEVAPSMDQMNELIIQHVVIRTLQDITLKNGQPALSIFDRNTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD
RSAAYTTRQIAKSIVVSKLAKRALVQLSYAIGVAKPLSIFVETYGSEQGALTADDITDIVKLAFDCRPGAIAKSLQLTEPKYVESAAYCHFGREPRTENGIKFFEWENPV
DLSS
>Phacus inflexus methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTCCTTTTCTCATCGGAATCCGTGAATGAAGGCCATCCTGACAAGCTCTGTGACCAGGTTTCAGACTCTGTGCTGGATGCTTGCCTTGCGGTCGACCCG
AAGAGCAAGGTGGCTTGTGAATGCGCGGTGAAGGACAACATGGTAATGGTTCTCGGGGAAATCACGACTCAGGCAAAGCTGGATTATGAGAAGATTGTC
CGGACTGCCGTGCGCAACATCGGCTTTGATTCCTTTGTTGACGACCTTTCCAGCGTTGACTCGAAGGGGCTGAACTGCGATTCCTGCGAGGTGTTGGTGCG
GATCAACAAGCAATCCCCTGATATTGCTGGGGGTGTCCACGTGGGCCGCGATGAGCTCGACTTCGGCGCTGGTGACCAAGGCATCATGTTTGGATATGCG
ACGGACGAGACCAAGAGCACCATGCCTTTGACGCACTACATGGCGACTCGCCTTGGGAAGACCCTGACCGACGTGCGCAAGAATGGTACGCTTTGGTGGC
TGCGCCCTGATGGCAAGACTCAGGTCACCATTAAGTACAAGCAGTGCGCTGATGGCTCTGTTGAGCCCTTAATGATCCACACGGTTCTTATCTCCACCCAGC
ACTGTGAACCCGGGAAGAGGAAGAAGGGTGAAGAAATTCAGGGCTACACAGGCCCTGATGCCAATGAGGTGGCCCCCAGCATGGAAGAAATGAACGAG
CAGATCATCCAGCACGTCATCATCAAGACGCTGCAGGACATCACCCTCAAGAACGGCCAGCCCGCTCTGAGCATTTTCGACCGGAAGACCTGCCATTTGCA
CATCAATCCCTCTGGCAAGTTCATCATTGGAGGCCCGCAAGGAGATGCCGGTCTGACAGGCCGCAAGATCATCATTGACACCTATGGAGGCTGGGGTGCC
CATGGTGGTGGTGCGTTCTCTGGGAAGGATCCGACCAAGGTGGACCGCTCTGCTGCTTACATTACCCGCCAGATTGCCAAGTCCATTGTGGTCTCCAAGTT
GGCGAAGCGTGCCCTGGTTCAGTTGTCTTATGCCATTGGTGTTGCCAAGCCCCTTTCCATCTTCGTTGAGACGTATGGCACGGAGCAGGGCGACCTCACTG
CCGATGACATCACGGACGTGGTGAAGCTCGCCTTCGATTGCCGCCCTGGCGCGATTGCCAAATCCCTGCAGCTCACCGAGCCTAAGTACGTGGAGACTGCA
GCTTATTGCCATTtCGGCCGGGAGCCACGCACCGAGAATGGCATCAAGTATTTTGAGTGGGAGAACCCGAAGGACCTGTCCAAG
>Phacus inflexus methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAVDPKSKVACECAVKDNMVMVLGEITTQAKLDYEKIVRTAVRNIGFDSFVDDLSSVDSKGLNCDSCEVLVRINKQS
PDIAGGVHVGRDELDFGAGDQGIMFGYATDETKSTMPLTHYMATRLGKTLTDVRKNGTLWWLRPDGKTQVTIKYKQCADGSVEPLMIHTVLISTQHCEPGKRK
KGEEIQGYTGPDANEVAPSMEEMNEQIIQHVIIKTLQDITLKNGQPALSIFDRKTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQIAKSIVVSKLAKRALVQLSYAIGVAKPLSIFVETYGTEQGDLTADDITDVVKLAFDCRPGAIAKSLQLTEPKYVETAAYCHFGREPRTENGIKYFEWEN
PKDLSK
>Phacus orbicularis methionine adenosyltransferase (MATX) mRNA, partial CDS
GGTCATCCAGACAAGCTCTGTGACCAAGTCTCTGATTCAGTGCTCGATGCTTGCCTGGCAGTGGACCCCAAAAGCAAAGTCGCATGTGAGACGGCTGTGA
AAGACAATATGGTGATGGTGCTTGGTGAAATTACCACACAAGCCAAGCTTGATTATGAAAAGATCGTCCGAACAGCAGTCAAAAACATTGGCTTCGATTCG
TTTGTGGATGACTTGTCCAGTGTTGATTCCAAGGGGTTGGACCACAAGACATGTGAGGTTCTAGTTCGCATCAACAAGCAGTCCCCTGACATCGCTGGAGG
TGTTCACATCGGTCGCGATGAATTGGATTTTGGTGCTGGAGACCAAGGAATTATGTTTGGCTATGCCTCAGATGAGACAAAAAGCACAATGCCCCTGACAC
ATTTTCTTGCCACGCGCCTCGGCAAAACGCTGACAGAAGTTCGCAAGAATGGCACTTTGTGGTGGCTGCGCCCGGACGGAAAGACTCAAGTCACCATTAA
GTACAAGCAATGCGCTGATGGTTCTGTGGAGCCACTGATGATTCACACAGTCCTGATTTCCACTCAACACTGTGAGCCAGGCAAAAGGAAAAAAGGCGAA
GAGATTCAAGGTTACACGGGAGCAGATGCAGGAGAAATTGCCCCCACCAAGGAGGAAATGGACGAGTTGATGATCCAGCACGTTGTCATCAAGACCTTG
CAAGACATCACGTTGAAAAATGGTCAACCTGCCCTGAGCATTTTTGACCGCAACACATGCCATTTGCACATGAACCCCTCAGGCAAATTCATCATTGGTGGT
CCTCAGGGAGATGCCGGACTCACTGGCCGTAAAATCATCATTGACACTTACGGTGGCTGGGGCGCTCACGGTGGCGGTGCCTTCTCTGGAAAAGATCCAA
CCAAGGTTGATCGGTCTGCAGCATACATCACAAGGCAGATTGCCAAGTCCATTGTTGTGTCAAACCTTGCTCGGCGTGCTCTTGTTCAACTGTCATACGCAA
TTGGGGTGGCGAAGCCCCTTTCAATTTTTGTCGAGACTTATGGATCAGAAAaGGGcGATTTGACTGCTGATGACATCACTGACATCGTGAAGTTGTCTTTCG
ATTGCCGACCTGGGGCAATTGCAAAGTCTCTTCAGCTCACTGAGCCAAAGTATGTCGAAACAGCAGCTTACTGCCACTTTGGCAGGGACCCTCGCACAGAG
AATGGCATCAAGTTCTTCGAGTGGGAGAACCCAGTTGATTTGTCCAAG
>Phacus orbicularis methionine adenosyltransferase (MATX) amino acid translation, partial protein
GHPDKLCDQVSDSVLDACLAVDPKSKVACETAVKDNMVMVLGEITTQAKLDYEKIVRTAVKNIGFDSFVDDLSSVDSKGLDHKTCEVLVRINKQSPDIAGGVHIG
RDELDFGAGDQGIMFGYASDETKSTMPLTHFLATRLGKTLTEVRKNGTLWWLRPDGKTQVTIKYKQCADGSVEPLMIHTVLISTQHCEPGKRKKGEEIQGYTGA
DAGEIAPTKEEMDELMIQHVVIKTLQDITLKNGQPALSIFDRNTCHLHMNPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSAAYITRQI
AKSIVVSNLARRALVQLSYAIGVAKPLSIFVETYGSEKGDLTADDITDIVKLSFDCRPGAIAKSLQLTEPKYVETAAYCHFGRDPRTENGIKFFEWENPVDLSK
> Lepocinclis tripteris methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTTCTTTTTTCCTCGGAATCAGTCAATGAGGGTCACCCTGACAAACTTTGTGACCAAGTGTCGGACTCCGTCTTGGATGCCTGCCTGAAGGAGGATCCC
AAGAGCAAGGTGGCATGTGAAACTGCTGTGAAGGACAACATGGTGATGGTGCTGGGAGAAATCACCACGCAAGCTAGGTTGGACTACGAGAAGATCGTG
AGGACTGCCGTGCGCAACATTGGTTTCGATTCTTTTGTGGACGACTTGTCCAGCGTGGATTCCAAGGGTTTGAACTGCGACACGTGTGAAGTGCTGGTCCG
CATCAACAAGCAGTCGCCCGACATTGCTGGCGGTGTACACGTCGGCAGAGATGACATGGATTTCGGGGCAGGTGACCAAGGAATCATGTTTGGTTATGCC
ACCGATGAAACCAAGAGTACCATGCCCCTCACCCACTATCTGGCAACCCGCCTTGGCAAAACTTTGACTGAGGTGCGCAAAAATGGAACACTGTGGTGGTT
GCGGCCAGATGGGAAAACCCAAGTGACAATCAAGTACAAGCAGTGCAAAGATGGCTCAGTGGAGCCATTGATGATCCACACCGTTCTCATTTCCACTCAG
CACTGCGAGCCAGGGAAAAGGAAGAAGGGCGAAGAGATCAAGGGCTACACAGGTGCCGATGCTGGTGAAGTTGCCCCCTCCATGGAACAAATGAATGA
GCTCATCATTCAGCACGTCATCATCAAGACCCTGCAGGACATCACCCTGAAGAACGGGCAGCCCGCCCTGACCATCTTCGACCGCAACACCTGCCACCTGCA
CATCAACCCCTCCGGGAAGTTCATCATCGGCGGGCCGCAGGGGGACGCCGGGCTCACCGGCCGCAAGATCATCATCGACACCTACGGCGGGTGGGGCGC
GCACGGTGGGGGGGCCTTCTCCGGAAAGGACCCCACCAAGGTGGACCGCTCCGCCGCGTACATCACCCGCCAGATGGCCAAGTCCATCGTCGTCAGCGG
GCTGGCGAAGCGAGCCCTGGTGCAGCTGTCGTATGCGATTGGCGTTGCCAAGCCCCTGTCCATTTTTGTGGAAACCTACGGTTCGGAGCAGGGGGCATTG
ACAGCTGCGGACATCACGGACATTGTGAAGCTCGCCTTCGACTGCCGGCCGGGCGCCATCGCCAAGTCCCTGCAGCTGACGGAGCCCAAGTACGTGGAGA
CCGCGGCATACTGCCACTTCGGCAGGGAGCCGCGCACCGAGAATGGCATCAAGTTCTTTGAATGGGAGAACCCCGTTGACCTGTCCAAG
>Lepocinclis tripteris methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLKEDPKSKVACETAVKDNMVMVLGEITTQARLDYEKIVRTAVRNIGFDSFVDDLSSVDSKGLNCDTCEVLVRINKQS
PDIAGGVHVGRDDMDFGAGDQGIMFGYATDETKSTMPLTHYLATRLGKTLTEVRKNGTLWWLRPDGKTQVTIKYKQCKDGSVEPLMIHTVLISTQHCEPGKRK
KGEEIKGYTGADAGEVAPSMEQMNELIIQHVIIKTLQDITLKNGQPALTIFDRNTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD
RSAAYITRQMAKSIVVSGLAKRALVQLSYAIGVAKPLSIFVETYGSEQGALTAADITDIVKLAFDCRPGAIAKSLQLTEPKYVETAAYCHFGREPRTENGIKFFEWENP
VDLSK
>Trachelomonas ellipsoidalis methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTCCTGTTTTCTTCTGAATCAGTCAATGAAGGTCACCCTGATAAGTTGTGTGATCAAGTGTCCGACTCTGTTTTGGATGCCTGTTTGGCAGTTGATCCAA
AGAGCAAGGTAGCTTGTGAAACTGCAGTGAAGGACAACATGGTCATGGTTCTTGGGGAAATCACCACCCAGGCAAAGTTGGATTACGAAAAGATTGTCCG
GACAGCAGTCAGAAACATTGGCTTTGATTCATTTGTTGATGACTTGTCCAGCGTTGATTCCAAGGGGTTAAATTGTGATGACTGTGAAGTTCTTGTGCGGAT
TAACAAGCAATCTCCTGACATTGCTGGAGGAGTGCACGTTGGGAGGGATGACATGGATGTTGGAGCTGGCGATCAAGGAATCATGTTTGGATATGCCACA
GATGAAACTCAGAGCACCATGCCACTGACACATTACCTTGCAACTCGCCTTGGGAAAACCCTCACGGAAGTGCGAAAGGATGGTTCTCTTTGGTGGTTGCG
TCCCGATGGAAAGACACAAGTGACAATCAAGTACAAGCAATGTGCAGATGGTTCTGTGGAGCCCCTGTCGATACACACAGTGCTTATTTCCACGCAGCACG
CTGAACCTGGCAAGAGAAAGAAGGGGGAGGAGATACGGGGTTACAAAGGGCCAGATGCTGATGAAGTGGCACCAAGCATGGAACAAATGAATGAATTG
ATCATTCAACATGTGGTCATTCGGACTCTGGAAAACATTACTTTGAAGAATGGGCAGCCAGCTTTGAGCCTCTTTGATCGCAACTCATGCCACCTTCACATC
AATCCTTCTGGCAAGTTCATCATTGGAGGCCCACAAGGTGATGCTGGGTTGACAGGGCGGAAGATCATCATTGACACATATGGTGGTTGGGGTGCCCATG
GTGGTGGTGCCTTTTCTGGGAAGGACCCCACCAAGGTTGACCGGTCTGCTGCATACATCACACGCCAGATTGCCAAGTCCATCGTTGTTTCCAAGCTTGCCA
AACGTGCTTTGGTGCAGCTGTCATATGCCATCGGGGTTGCCAAGCCTCTCTCTATCTTTGTGGAGACCTATGGGTCAGAGCAGGGGGCCCTGACAGCAAGT
GACATCACAGATATTGTGAAGCTTGCATTCGATTGCCGCCCTGGCGCCATTGCCAAGTCATTGCAGCTGACAGAGCCCAAATATGTGGAGACTGCTGCGTA
TTGCCATTTTGGGAGGGAGCCCCGCACTGAGAATGGCATCAAGTATTTTGAGTGGGAGAACCCAGTGGACCTCTCCAAA
>Trachelomonas ellipsoidalis methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAVDPKSKVACETAVKDNMVMVLGEITTQAKLDYEKIVRTAVRNIGFDSFVDDLSSVDSKGLNCDDCEVLVRINKQS
PDIAGGVHVGRDDMDVGAGDQGIMFGYATDETQSTMPLTHYLATRLGKTLTEVRKDGSLWWLRPDGKTQVTIKYKQCADGSVEPLSIHTVLISTQHAEPGKRK
KGEEIRGYKGPDADEVAPSMEQMNELIIQHVVIRTLENITLKNGQPALSLFDRNSCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQIAKSIVVSKLAKRALVQLSYAIGVAKPLSIFVETYGSEQGALTASDITDIVKLAFDCRPGAIAKSLQLTEPKYVETAAYCHFGREPRTENGIKYFEWENP
VDLSK
>Euglena stellata methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTCCTTTTCTCTTCCGAATCTGTTAATGAAGGTCATCCTGACAAGCTTTGCGACCAGGTTTCAGACTCCGTATTGGATGCTTGCCTCGCTCAAGATCCCA
AGAGTAAAGTTGCTTGTGAAACAGCCGTGAAGGATAATATGGTGATGGTTTTGGGAGAAATTACAACCCAAGCCAAGCTGGACTATGAGGCAATTGTCCG
CCAGGCCGTCCGAAATATCGGCTTTGATTCCTTTGTTGACGACCTTGCCAGTGTTGATTCCAAGGGGCTGAACTGTGATGATTGTGAAGTGCTTGTCCGTAT
CAACAAGCAATCCCCAGATATTGCTGGTGGAGTGCACGTTGGTAAGGATGAGATGGATGTGGGTGCTGGAGATCAGGGAATTATGTTTGGATATGCTTCT
GATGAGACCAAGAGCACCATGCCCTTGACTCACTACATGGCTACCCGACTGGGCAAAACGCTCACTGAAGTTCGCAAGAATGGCACCCTCTGGTGGTTGC
GTCCTGATGGCAAGACCCAGGTGACCATCAAGTACAAGCAATGCGCTGATGGGTCAGTTGAGCCTCTGATGATCCACACCGTGCTGATCTCCACCCAGCAC
TCTGAACCCGGCAAGCGGACCAAGGGAGAGGAAATCCGCGGGTACAAGGGTGCCGATGCCGGTGAGGTAGCCCCAAGCATGGAGCAGATGAACGAGCT
CATCATCCAGCACGTTGTCGTTAAAACCTTGGAGGAGATCACTCTGAAGAACGGTCAATCCGCTCTGAGCATCTTCGACCGCAACACTTGCCATCTGCACAT
CAACCCCTCTGGCCGGTTCATCATTGGTGGACCACAGGGAGACGCTGGTCTGACTGGCCGCAAGATCATCATTGACACCTATGGCGGCTGGGGAGCACAT
GGTGGTGGAGCTTTCTCTGGCAAGGACCCGACCAAGGTTGACCGGTCGGCCGCTTACATCACCCGCCAGATCGCTAAGTCCATCGTAGTCTCCGGCCTGGC
CAAGCGAGTGCTGGTGCAGCTCTCGTACGCCATCGCCGTTGCTAAGCCTTTGTCCGTCTTTGTTGAGACCTATGGCTCAGAGCAGGGCGCTCTGACCGCCG
ATGACATCACGGATATCGTGAAGCTGAACTTCGACTGCCGTCCCGGAGCGATCGCTAAATCACTGCAGCTGCGAGAGCCCAAGTACACCGAATCCGCCGCC
TACTGCCACTTTGGGCGGGAGCCACGCACCGAGAACGGCATCAAGTATTTTGAGTGGGAGAACCCCGTTGATCTGTCCAAG
>Euglena stellata methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAQDPKSKVACETAVKDNMVMVLGEITTQAKLDYEAIVRQAVRNIGFDSFVDDLASVDSKGLNCDDCEVLVRINKQ
SPDIAGGVHVGKDEMDVGAGDQGIMFGYASDETKSTMPLTHYMATRLGKTLTEVRKNGTLWWLRPDGKTQVTIKYKQCADGSVEPLMIHTVLISTQHSEPGK
RTKGEEIRGYKGADAGEVAPSMEQMNELIIQHVVVKTLEEITLKNGQSALSIFDRNTCHLHINPSGRFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPT
KVDRSAAYITRQIAKSIVVSGLAKRVLVQLSYAIAVAKPLSVFVETYGSEQGALTADDITDIVKLNFDCRPGAIAKSLQLREPKYTESAAYCHFGREPRTENGIKYFEW
ENPVDLSK
> Euglena hiemalis methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTTCTCTTCTCCTCTGAGTCAGTCAATGAGGGTCATCCGGATAAGCTGTGCGACCAGGTTTCAGACTCTGTTCTGGATGCCTGCTTGGCTCAGGACCCA
CTCAGCAAAGTGGCTTGTGAGGCAGCTGTGAAGGATAATATGGTCATGGTTCTGGGTGAAATCACCACTCAAGCCAAGCTGGACTACGATGCCATTGTGC
GCAATGCTGTCCGCAACATCGGCTTTGATGCATTTGTTGATGACTTGAGCAGTGTGGACTCCAAGGGGTTGAACTGTGATGATTGTGAGGTCCTTGTCCGG
ATCAACAAGCAGTCCCCAGACATTGCTGGCGGTGTGCACGTTGGTCGTGATGAGATGGATGTTGGAGCTGGAGATCAGGGCATTATGTTTGGCTATGCCA
CTGATGAAACTGAGAGCACCATGCCCTTGACACACTACGTTGCTACTCGCCTTGGCAAGACGCTGACCGAGGTGCGCAAGGATGGAAGTTTGTGGTGGTT
GCGTCCTGATGGCAAGACGCAGGTGACCATCAAGTACAAGCAGTGCGCGGATGGAGCCGTGGAGCCACTAATGATCCACACCATCCTGATCTCCACCCAG
CACGTGGAGCCGGGCAAGCGCAAGAAGGGGGAGGAGCTCAAGGGGTACAAGGGGCCCGACGCGGAGGAGGTGGCCCCAAGCATGGAGCAGATGAAC
GAGCTGATGATCCAGCACGTCGTGATCCGCACCCTGGAGAACATCACGCTGAAGAACGGGAAGCCGGCCATCAGCATCTTCGACCGCAGCACGTGCCACC
TGCACATGAACCCGTCAGGGAAGTTCATCATCGGCGGCCCGCAGGGGGACGCCGGCCTCACCGGCCGCAAGATCATCATTGACACCTACGGCGGGTGGG
GCGCTCACGGGGGCGGGGCCTTCTCTGGCAAGGACCCCACCAAGGTGGACCGCTCCGCGGCCTACATCACCCGCCAGATCGCCAAGTCCATCGTCGTCTC
CAAGCTGGCGCGCCGGGCACTGGTGCAGCTGTCCTACGCCATCGCCGTCGCCAAGCCCCTGTCCGTGTTCGTGGAGACGTACGGGTCGGAGCAGGGCGCC
CTGACCGCCGACGACATCACCGACATCGTCAAGCTGAACTTCGACTGCCGCCCCGGTGCCATCGCCAAGTCTCTGCAGCTGCGGGAACCGAAGTACGTGG
AGTCTGCGGCCTACTGCCACTTCGGACGGGAGCCCCGCACCGAGAACGGCATCAAGTATTTTGAGTGGGAGAACCCGGTGGACCTCTCCAAG
>Euglena hiemalis methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAQDPLSKVACEAAVKDNMVMVLGEITTQAKLDYDAIVRNAVRNIGFDAFVDDLSSVDSKGLNCDDCEVLVRINKQ
SPDIAGGVHVGRDEMDVGAGDQGIMFGYATDETESTMPLTHYVATRLGKTLTEVRKDGSLWWLRPDGKTQVTIKYKQCADGAVEPLMIHTILISTQHVEPGKR
KKGEELKGYKGPDAEEVAPSMEQMNELMIQHVVIRTLENITLKNGKPAISIFDRSTCHLHMNPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPT
KVDRSAAYITRQIAKSIVVSKLARRALVQLSYAIAVAKPLSVFVETYGSEQGALTADDITDIVKLNFDCRPGAIAKSLQLREPKYVESAAYCHFGREPRTENGIKYFEW
ENPVDLSK
> Lepocinclis playfairiana methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTTTTATTTTCTTCCGAGTCTGTGAACGAAGGCCATCCCGACAAGCTTTGCGACCAAGTATCCGATTCAGTTCTTGATGCTTGCTTGCGTGAGGATCCAC
TGAGCAAAGTGGCCTGTGAGACTGCTGTGAAGGACAATATGGTAATGGTGCTTGGAGAAATTACAACCAACGCTAAATTGGACTATGAAAAGATTGTCCG
CGATGCTGTCCGTAACATTGGCTTTGATTCCTTCGTGGACGATCTGTCAAGTGTGGATTCGAAGGGTTTGAATTGTGAAGACTGTGAAGTTTTGGTTCGCAT
AAACAAGCAATCTCCTGACATTGCAGGGGGTGTTCATGTTGGCCGAGATGAAATGGATGTTGGTGCTGGGGATCAGGGAATTATGTTTGGATATGCCTCA
GATGAAACCAAGAGCACCATGCCTCTTACTCATTACTTGGCGACAAGGCTTGGGAAGACATTGACTGAAGTAAGGAAAAGTGGGCAGCTATGGTGGCTGC
GCCCCGATGGGAAGACTCAGGTCACCATTAAGTACAAGCAAAATGCAGATGGCTCAGTTGAACCCCTGCTGATCCACACAGTCCTAATTTCCACGCAGCAC
TGTGAACCTGGAAAGAGGAAGAAGGGTGAAGAAATTAAGGGTTACAAGGGAGCTGATGCTGACGATGTGGCTCCTTCGATGGAACAAATGAATGAGCTT
ATCATCGAGCACGTGATCATCAAAACCCTTCAAGACATCACACTGAAGAATGGAAAACCTGCTTTATCAATCTTTGACCGCAGCACTTGCCACTTGCATATC
AATCCTTCTGGGAAGTTCATCATTGGCGGACCCCAGGGAGATGCTGGTCTGACCGGAAGGAAAATCATCATTGATACCTATGGAGGCTGGGGAGCTCATG
GCGGTGGAGCATTCTCTGGGAAAGATCCCACCAAAGTGGACCGATCTGCCGCATACATTACCCGCCAAATCGCCAAGTCTGTCGTTGCCAGTGGACTTGCA
CGCCGAGCACTGGTTCAATTATCTTATGCCATTGGGGTTGCTAAACCTTTATCCATCTTTGTGGAGACCTATGGCTCGGAGCAGGGCAAATTGACTGCTGAT
GACATCACTGACATTGTTAAGCTTGAATTTGACTGCCGTCCTGGGGCCATTGCGAAGTCTTTGCAGCTCAGGGAACCCAAGTACGTTGAGACTGCTGCATA
CTGCCACTTTGGGCGAGAGCCCCGTACAGAGAATGGCATCAAGTACTTCGCTTGGGAGAACCCTGTTGACTTGTCCAAA
>Lepocinclis playfairiana methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLREDPLSKVACETAVKDNMVMVLGEITTNAKLDYEKIVRDAVRNIGFDSFVDDLSSVDSKGLNCEDCEVLVRINKQS
PDIAGGVHVGRDEMDVGAGDQGIMFGYASDETKSTMPLTHYLATRLGKTLTEVRKSGQLWWLRPDGKTQVTIKYKQNADGSVEPLLIHTVLISTQHCEPGKRKK
GEEIKGYKGADADDVAPSMEQMNELIIEHVIIKTLQDITLKNGKPALSIFDRSTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDR
SAAYITRQIAKSVVASGLARRALVQLSYAIGVAKPLSIFVETYGSEQGKLTADDITDIVKLEFDCRPGAIAKSLQLREPKYVETAAYCHFGREPRTENGIKYFAWENPV
DLSK
>Euglenaria anabaena methionine adenosyltransferase (MATX) mRNA, partial CDS
GTCAATGAGGGCCACCCAGACAAGCTGTGCGATCAGGTGTCGGACTCCGTGCTGGATGCCTGCCTGGCTCAGGACCCCAAGAGCAAGGTGGCCTGTGAA
ACGGCTGTGAAGGACAACATGGTCATGGTACTCGGGGAGATCACCACACAGGCCAAGCTTGACTACGAGAAGATCGTGCGTACAGCAGTGCGTAACATC
GGCTTCGACTCGTTCATTGACGACCTTACCAGTGTGGAGTCGAAGGGGCTGGACTGCGACAACTGCGAGGTGCTGGTGCGGATCAACAAGCAGTCCCCCG
ACATTGCGGGAGGCGTGCATGTTGGTCGCGACGATATGGACGTGGGCGCTGGCGATCAGGGCATTATGTTCGGCTACGCCACAGACGAGACGCAGAGCA
CGATGCCCCTGACGCACTACATGGCTACCAGGCTGGGCAAGACCTTGACGGAAGTTCGCAAGGACGGCACCCTGTGGTGGCTGCGCCCCGACGGCAAGA
CGCAGGTGACCATCAAGTACCGGCAGTGCGCGGATGGGTCGGTGGAGCCGCTGCTGATCCACACAGTGCTCATCTCTACCCAGCACTGTGAGCCCGCGAA
GCGCACGAAGGGGGAGGAGATCAAGGGCTACAAGGGGTCCGATGCCGACGAGGTTGCCCCGTCGATGGAGGACATGAACGAGCTGATCGTGCAGCACG
TCGTGATCCGCACCCTGCAGAACATCACCCTGAAGAACGGAAAGCCTGCGCTGTCCATCTTCGACCGGAACACCTGCCATTTGCACATCAACCCGTCTGGG
AAGTTCATCATCGGCGGGCCCCAGGGCGACGCGGGGCTGACGGGCCGGAAGATCATCATCGACACCTACGGCGGGTGGGGCGCCCACGGCGGCGGGGC
GTTCTCCGGGAAGGACCCCACCAAGGTGGACCGCTCCGCGGCGTACATCACGCGGCAGATCGCGAAGTCCATCGTGGTGTCCAATTTGGCGCGGCGTGCT
CTGGTGCAGCTGTCCTACGCCATCGGGGTGGCCAAGCCCCTCTCCGTCTTCGTGGAGACCTACGGGTCGGAGCAGGGCGACCTAACGGCGGCGGACATCA
CGGACATCGTGAAGCTTGCATTCGACTGTCGCCCGGGGGCTATCGGGAAGTCACTGCAGCTGAGGGAGCCGAAGTACACGGAGACGGCGGCGTACTGCC
ACTTCGGGCGGGAGCCCCGCGTTGAGGGCACCATCAAGTTCTTCGAGTGGGAGCGGCCGGTGGACCTATCCAAG
>Euglenaria anabaena methionine adenosyltransferase (MATX) amino acid translation, partial protein
VNEGHPDKLCDQVSDSVLDACLAQDPKSKVACETAVKDNMVMVLGEITTQAKLDYEKIVRTAVRNIGFDSFIDDLTSVESKGLDCDNCEVLVRINKQSPDIAGGV
HVGRDDMDVGAGDQGIMFGYATDETQSTMPLTHYMATRLGKTLTEVRKDGTLWWLRPDGKTQVTIKYRQCADGSVEPLLIHTVLISTQHCEPAKRTKGEEIKG
YKGSDADEVAPSMEDMNELIVQHVVIRTLQNITLKNGKPALSIFDRNTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSAAYI
TRQIAKSIVVSNLARRALVQLSYAIGVAKPLSVFVETYGSEQGDLTAADITDIVKLAFDCRPGAIGKSLQLREPKYTETAAYCHFGREPRVEGTIKFFEWERPVDLSK
>Monomorphina parapyrum methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTCCTCTTCTCCTCCGAGTCAGTCAATGAGGGTCACCCAGACAAACTGTGCGATCAGGTCTCGGACTCAGTGCTTGATGCCTGCCTCAAGGTGGATCCC
AAAAGCAAGGTGGCCTGTGAGACAGCTGTGAAGGACAACATGGTGATGGTGCTCGGCGAGATCACCACCCAGGCGAAGCTGGACTATGAGGCCATCGTG
CGCACCGCTGTCCGCAACATTGGATTCGACTCGTTCGTGGACGACCTGAGCAGCGTGGATTCCAAGGGACTGAACTGTGATGATTGTGAAGTGTTGGTGC
GCATCAACAAGCAGTCGCCCGACATTGCAGGCGGGGTGCACGTGGGGCGCGATGACATGGACTTTGGAGCGGGCGACCAGGGCATCATGTTCGGGTACG
CCACGGACGAGACTCAGAGCACGATGCCGCTGACGCACTACCTTGCCACGCGCCTCGGGCGCACGCTGACGGAGGTGCGCAAGAACGGCACGCTGTGGT
GGCTGCGCCCCGACGGGAAGACGCAGGTGACAATCAAGTACAGGCAGTGCGCCGACGGCTCCGTGGAGCCGCTGATGATCCACACCGTGCTGATCTCCA
CACAGCACTGCGAGCCCGGCAAGCGCACGAAGGGCGCGGAGATCAAGGGCTACAAGGGCGCGGACGCCGGCGAGGTGGCCCCGTCGATGGAGCAGAT
GAACGAGCTGATCATCCAGCACGTTGTGATCCGCACCCTGCAGGACATCACGTTGAAGAACGGCAAGCCGGCGCTGAGCATCTTCGACCGCAAGACGTGC
CACCTGCACATCAACCCCTCCGGCAAGTTCATCATCGGCGGCCCCCAGGGCGACGCCGGGCTGACCGGCCGCAAGATCATCATCGACACCTATGGTGGCT
GGGGTGCGCACGGTGGTGGCGCCTTCTCTGGCAAGGACCCCACCAAGGTGGACCGCTCCGCGGCATACATCACGCGCCAGATCGCCAAGTCCATCGTCGT
CTCGCGCCTCGCGAAGCGCGCGCTGGTGCAGCTGTCGTACGCCATCGGTGTTGCCAAGCCGCTCTCCATCTTCGTCGAGACGTACGGGTCGGAGATGGGC
GAGCTGACGGCCGATGACATCACGGACATCGTGAAGCTGGCGTTCGACTGCCGCCCCGGCGCGATCGCCAAGTCGCTGCAGCTGACCGAGCCCAAGTACA
CGGAGACGGCGGCGTACTGCCACTTCGGGCGCGAGCCGCGCACCGAGAACGGCATCAAGTTCTTCGAGTGGGAGAACCCCGTGGACCTGTCCGCC
>Monomorphina parapyrum methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLKVDPKSKVACETAVKDNMVMVLGEITTQAKLDYEAIVRTAVRNIGFDSFVDDLSSVDSKGLNCDDCEVLVRINKQS
PDIAGGVHVGRDDMDFGAGDQGIMFGYATDETQSTMPLTHYLATRLGRTLTEVRKNGTLWWLRPDGKTQVTIKYRQCADGSVEPLMIHTVLISTQHCEPGKRT
KGAEIKGYKGADAGEVAPSMEQMNELIIQHVVIRTLQDITLKNGKPALSIFDRKTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQIAKSIVVSRLAKRALVQLSYAIGVAKPLSIFVETYGSEMGELTADDITDIVKLAFDCRPGAIAKSLQLTEPKYTETAAYCHFGREPRTENGIKFFEWENP
VDLSA
> Eutreptiella braarudii methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTCCTTTTCTCCTCTGAGTCTGTGAATGAGGGTCACCCTGATAAGCTGTGCGACCAGGTTTCCGACTCCGTTCTGGATGCCTGCTTGACCGCTGACCCC
CTGAGCAAGGTGGCCTGCGAGACTGCCGTGAAGGACAACATGGTCATGGTTCTTGGTGAGATCACCACCGGCGCCAAGTTGGACTACGAGGCCATTGTTC
GCAAGGCTTGCCGCCACATCGGTTTCGACTCCTATGTGGATGACTTGTCCAGTGTGGAGTCCAAGGGTTTGAACTGCGATGACTGCGAGGTGCTTGTCCGT
ATCAACGCTCAGTCTCCCGACATTGCTGGCGGTGTGCACGTTGGCCGTGACGAGATGGACGTTGGTGCTGGTGACCAGGGTATTATGTTCGGATATGCCTC
TGACGAGACCAAGAGCACCATGCCTTTGACTCACTATCTTGCCACCAAGCTGGGCAAGACCCTCACTGACGTTCGCAAGAACGGCAAGTTGTGGTGGTTGC
GACCTGACGGTAAGACTCAGGTGACCATCAAGTACAAGCAGCACGCTGACGGTTCCGTGGAGCCTCTTATGATTCACACCGTGCTGATCTCCACCCAGCAC
GCCGAGCCTCTGAAGAGGACCAAGGGTGCCGAGATCGCTGGCTACAAGGGTGCGGACGCCGGTGAGGTGGCCCCTTCCATGGAGGACATGAACAAGCA
GATCCTTGAGCAGGTCATCAAGGCAACCCTTCGTGACATCACGTTGAAGAACGGCAAGCCTGCCATCAGCATCTTTGACGAGAAGACCTGCCACACCCACA
TCAACCCATCTGGCAAGTTCATCATCGGTGGTCCTCAGGGCGATGCCGGTCTTACTGGCCGTAAGATCATCATCGACACCTATGGTGGTTGGGGTGCCCAC
GGTGGTGGTGCTTTCTCCGGCAAGGACCCCACCAAGGTGGACCGCTCTGCTGCCTACATCACCCGCCAGATGGCTAAGTCCATCGTGACTTCCGGACTTGC
CAAGCGCGCCCTGGTGCAGCTTTCCTACGCCATTGGTGTGGCCAAGCCTCTGTCCGTGTTCGTCGAGACCTACGGCTCCGAGCAGGGTGCCTTGACTGCTG
ATGATATCACTGACGTCATCAAGCTTGCCTTCGACTGCCGCCCTGGTGCCATCGCCAAGTCCTTGGCCCTGCGTGAGCCCAAGTACGTGGAGAGTGCAGCT
TACTGCCATTTCGGCCGTGAGCCTCGCACCGAGAACGGCATCAAGTACTTCGAGTGGGAGAACCCTGTGGATCTCTCCAAG
>Eutreptiella braarudii methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLTADPLSKVACETAVKDNMVMVLGEITTGAKLDYEAIVRKACRHIGFDSYVDDLSSVESKGLNCDDCEVLVRINAQS
PDIAGGVHVGRDEMDVGAGDQGIMFGYASDETKSTMPLTHYLATKLGKTLTDVRKNGKLWWLRPDGKTQVTIKYKQHADGSVEPLMIHTVLISTQHAEPLKRT
KGAEIAGYKGADAGEVAPSMEDMNKQILEQVIKATLRDITLKNGKPAISIFDEKTCHTHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQMAKSIVTSGLAKRALVQLSYAIGVAKPLSVFVETYGSEQGALTADDITDVIKLAFDCRPGAIAKSLALREPKYVESAAYCHFGREPRTENGIKYFEWE
NPVDLSK
>Trachelomonas volvocina methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTTTTGTTTTCCTCTGAGTCAGTCAATGAGGGACATCCTGACAAGCTGTGTGATCAAGTTTCAGACTCTGTGCTGGATGCTTGTCTTGCAGCTGACCCC
AAAAGCAAGGTGGCATGTGAAACAGCCGTGAAGGATAACATGGTGATGGTGTTGGGTGAGATCACCACCCAGGCCAAGCTGGACTATGAAAAGATTGTC
CGCAGTGCAGTCCGTAACATCGGATTCGATTCCTTTGTGGATGATTTGTCCAGCGTGGATTCCAAGGGGCTGAACTGCGATGATTGTGAAGTGTTGGTCCG
CATCAACAAGCAGTCTCCGGACATTGCTGGGGGCGTGCATGTTGGACGAGATGACATGGATGTGGGTGCTGGGGACCAGGGTATCATGTTTGGCTATGCC
TCTGATGAGACTCAAAGCACCATGCCCCTCACCCACTACTTGGCAACCCGCTTGGGCAAGACCCTGACCGACGTTCGCAAGAATGGCACCTTGTGGTGGTT
GCGCCCCGATGGCAAGACCCAGGTCACAATCAAGTATCGCCAGTGTGCTGATGGTGCTGTTGAACCCTTGATGATCCACACAGTGCTGATCTCCACCCAGC
ATGCCGAGCCAGGGAAGCGCACGAAGGGGGAGGAGATCCGTGGCTACAAGGGGGCAGATGCAAACGAAGTGGCCCCATCGATGGACCAGATGAATGA
ATTGATCATCCAGCACGTCGTCATTCGTACATTGGAGGACATCAAGCTGAAGAACGGCAAGCCAGCCCTCAGCATCTTCGACCGCAAGACATGCCATTTGC
ACATCAACCCATCGGGCAAGTTCATTATCGGAGGCCCACAGGGAGACGCCGGGTTGACGGGACGCAAGATCATCATCGACACCTATGGCGGATGGGGTG
CGCATGGTGGTGGTGCATTCTCCGGAAAGGACCCCACAAAGGTCGACCGATCAGCAGCATACATCACACGCCAGATCGCAAAGTCCATCGTCGTTTCCAAG
CTTGCCCGCCGGGCCCTCGTTCAGCTGTCCTATGCCATCGGTGTGGCAAAACCACTGTCCGTCTTTGTGGAGACATACGGATCGGAGCAAGGCAGCTTGAC
GGCTGATGACATCACCGATATCGTGAAGTTGGCGTTTGACTGCCGCCCCGGCGCCATCGCCAAGTCCCTGCAACTGACCGAGCCAAAGTATGTGGAGACC
GCGGCGTACTGCCATTTCGGGCGGGAACCACGGACACAGAACGGCATCAAGTTCTTCGAATGGGAGAACCCCGTTGATTTGTCCAAG
>Trachelomonas volvocina methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAADPKSKVACETAVKDNMVMVLGEITTQAKLDYEKIVRSAVRNIGFDSFVDDLSSVDSKGLNCDDCEVLVRINKQS
PDIAGGVHVGRDDMDVGAGDQGIMFGYASDETQSTMPLTHYLATRLGKTLTDVRKNGTLWWLRPDGKTQVTIKYRQCADGAVEPLMIHTVLISTQHAEPGKR
TKGEEIRGYKGADANEVAPSMDQMNELIIQHVVIRTLEDIKLKNGKPALSIFDRKTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQIAKSIVVSKLARRALVQLSYAIGVAKPLSVFVETYGSEQGSLTADDITDIVKLAFDCRPGAIAKSLQLTEPKYVETAAYCHFGREPRTQNGIKFFEWEN
PVDLSK
>Eutreptia viridis methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTCTTGTTCTCCTCTGAATCTGTTAACGAGGGTCATCCTGATAAGCTCTGCGACCAAGTGTCCGACTCGGTTCTAGATGCCTGCCTGAAAGAGGATCCT
CTCAGCAAGGTTGCCTGTGAAACCGCTGTCAAGGACAACATGGTGATGGTCTTGGGTGAAATCACCACCAAGGCCAAGTTGGACTACGAAGCGATTGTGC
GCAAGGCAGTCCGCAACATCGGTTTCGACTCGTTTGTTGACGACCTTGCCAGTGTCGACTCGAAGGGTCTGAACTGTGACGATTGCGAGGTTCTTGTTCGC
ATCAACAAGCAGTCTCCTGATATTGCAGGTGGTGTGCACGTTGGTCGCGACGAGATGGATGTTGGAGCCGGGGATCAAGGTATTATGTTTGGTTATGCCA
CTGACGAAACCAAGAGCACGATGCCCTTGACCCACTACCTTGCCACGAAACTGGGAAAGACTCTCACCGACGTTCGGAAGAGTGGTGAGCTTTGGTGGTT
GCGCCCTGATGGCAAGACCCAGGTTACCATCAAGTACAAACAATGTGCCGACGGCTCGGTAGAGCCTCTGATGATCCACACGATCTTGATCTCCACTCAGC
ATGCCGAGCCTATGAAGAGGACCAAGGGTGCCGAAATAGCCGGTTACAAGGGTGCTGACTCTGGAGAAACCGCTCCTTCAATGGAGAAGATGAACGAAC
TCATTGTAGAGTATGTAGTTAAGAGGACGCTGAAGGAGATCACGTTGAAGAACGGCGAGCCTGCCCTCAGCATCTTCGATGAGAAGACTTGCCACTTGCA
CATCAACCCATCTGGCAAGTTCATCATTGGTGGTCCACAAGGTGATGCCGGTCTCACTGGTCGCAAGATCATCATTGACACCTATGGAGGCTGGGGTGCCC
ATGGAGGTGGTGCCTTCTCTGGCAAGGATCCCACCAAGGTTGATCGTTCCGCTGCCTACATCACCCGTCAAATGGCAAAGTCTATTGTGACCTCTGGACTTG
CAAAACGTGCTCTCGTGCAGCTTTCATATGCCATTGGTGTTGCAAAGCCGCTTTCCATTTTTGTTGAGACCTACGGGTCCGAGCTCGGCGAGTTGACAGCTG
ACGACATCACAGACGTCATCAAGCTTGCATATGACTGCCGGCCAGGTGCCATTGCTAAACATTTGCAGCTGACTGAACCCAAGTACGTCGAGACAGCAGCT
TACTGCCACTTTGGACGAGAGCCTCGTACTGAAAATGGCATCAAATATTTTGAGTGGGAGAATCCAGTTGACCTTTCGAAG
>Eutreptia viridis methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLKEDPLSKVACETAVKDNMVMVLGEITTKAKLDYEAIVRKAVRNIGFDSFVDDLASVDSKGLNCDDCEVLVRINKQS
PDIAGGVHVGRDEMDVGAGDQGIMFGYATDETKSTMPLTHYLATKLGKTLTDVRKSGELWWLRPDGKTQVTIKYKQCADGSVEPLMIHTILISTQHAEPMKRT
KGAEIAGYKGADSGETAPSMEKMNELIVEYVVKRTLKEITLKNGEPALSIFDEKTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVD
RSAAYITRQMAKSIVTSGLAKRALVQLSYAIGVAKPLSIFVETYGSELGELTADDITDVIKLAYDCRPGAIAKHLQLTEPKYVETAAYCHFGREPRTENGIKYFEWENP
VDLSK
>Euglena proxima methionine adenosyltransferase (MATX) mRNA, partial CDS
GAATTCCTCTTCTCTTCAGAATCTGTGAACGAAGGACATCCTGACAAGCTTTGTGATCAGGTCTCTGATTCAGTTTTAGATGCTTGCCTTGCGGTTGATCCAA
AAAGTAAGGTGGCCTGCGAAACCGCAGTCAAGGACAACATGGTCATGGTTCTTGGTGAAATTACGACCCAAGCTAAGTTGGACTACGAAACTATTGTTCG
GACAGCAGTACGCAACATTGGCTTTGACTCTTTCATAGATGATCTTTCAAGTGTGGACTCTAAGGGCTTGAACTGTGATGATTGTGAGGTCCTGGTGCGCAT
CAACAAGCAATCTCCTGACATTGCCGGAGGTGTGCACGTTGGCCGCGATGAAATGGACTTTGGGGCTGGCGATCAAGGAATCATGTTTGGGTATGCCAGT
GATGAGACCAAGAGTACCATGCCTCTTACCCACTATCTTGCAACACGCCTGGGCAAAACATTGACCGAAGTTCGCAAAGATGGATCTCTATGGTGGCTGCG
ACCTGATGGGAAGACTCAGGTCACCATCAAGTATAGACAATGTGCAGATGGTTCCGTCGAACCACTCCTCATCCACACTGTCCTGATTTCCACTCAACACGC
TGAACCTGGCAAGCGCAAGAAAGGAGAAGAGATCAAAGGGTACAGCGGTCCCGATGCAGATGAATTGGCCCCATCAATGGATAAGATGAATGAGCTCAT
CATTCAGCACGTCGTAATCCGAACGTTGCAGAATATCACACTGAAGAATGGCGAACCCGCTCTTAGCATCTTCAACCGAGACACATGCCACCTGCACATCAA
CCCCTCTGGCAAATTCATCATTGGAGGTCCCCAAGGAGACGCTGGGTTGACCGGAAGGAAGATCATCATTGACACATATGGTGGCTGGGGTGCCCACGGA
GGTGGTGCCTTTTCTGGCAAGGATCCAACCAAGGTGGATCGATCAGCAGCATACATCACTCGCCAAATTGCCAAGTCCATTGTGACTTCTCAGCTGGCGAA
ACGTGCCTTGGTGCAATTATCCTATGCCATCGGGGTGGCAAAGCCTCTGTCCATTTTTGTTGAGACCTATGGATCTGAGCAAGGGAGCCTCACCGCCGATG
ACATCACCGACATCGTAAAATTGGCATTTGATTGCCGTCCAGGTGCCATTGCTAAATCTCTGCAACTGACAGAGCCGAAGTACGTCGAAACCGCAGCTTATT
GCCATTTTGGCCGCGAGCCACGCACCGAGAATGGGATCAAGTTCTTTGAGTGGGAAAACCCCGTTGATCTGTCGGCT
>Euglena proxima methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAVDPKSKVACETAVKDNMVMVLGEITTQAKLDYETIVRTAVRNIGFDSFIDDLSSVDSKGLNCDDCEVLVRINKQS
PDIAGGVHVGRDEMDFGAGDQGIMFGYASDETKSTMPLTHYLATRLGKTLTEVRKDGSLWWLRPDGKTQVTIKYRQCADGSVEPLLIHTVLISTQHAEPGKRKK
GEEIKGYSGPDADELAPSMDKMNELIIQHVVIRTLQNITLKNGEPALSIFNRDTCHLHINPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDR
SAAYITRQIAKSIVTSQLAKRALVQLSYAIGVAKPLSIFVETYGSEQGSLTADDITDIVKLAFDCRPGAIAKSLQLTEPKYVETAAYCHFGREPRTENGIKFFEWENPVD
LSA
>Strombomonas accuminata methionine adenosyltransferase (MATX) mRNA, partial CDS
GAGTTCCTTTTTTCCTCTGAATCAGTTAATGAAGGACATCCAGACAAGCTCTGTGACCAAGTGTCAGATTCCGTCCTTGATGCTTGCCTTGCTCAGGACCCGA
AGAGCAAAGTGGCTTGTGAGACAGCAGTCAAGGATAATATGGTGATGGTGCTGGGTGAAATTACAACACAGGCAAAATTGGATTATGAGGCCATCGTTC
GCAAGGCGGTGCGTAACATTGGTTTCGATTCCTTTGTTGATGACTTGGACAGCGTGGATTCCAAGGGGCTGAATTGTGACGATTGTGAGGTGCTGGTGCG
AATCAACAAGCAATCTCCTGATATTGCAGGAGGGGTGCATGTGGGGCGTGATGAAATGGACCTGGGTGCTGGAGACCAGGGCATCATGTTTGGATATGC
CAGTGATGAGACGAAGAGCACAATGCCACTGACTCATTACTTGGCAACAAGGTTGGGGAAAACCTTGACGGAGGTGCGGAAGGATGGTACGCTGTGGTG
GTTGCGGCCTGATGGCAAGACCCAGGTTACCATCAAGTACAAGCAGTGCGCAGATGGTTCAGTGGAGCCACTGCAAATTCATTCAGTGCTGATCTCCACAC
AGCACGTGGAACCTGGCAAGCGGAAGAAGAAGGAGGAGATCCGTGGATACCAGGGGGCCGATGGGGATGAAGTGGCACCCACGATGAAGGAAATGAA
TGAGCACTTGATCGAGCACGTGGTCATCCGCACATTGAAGGGCATCACTCTGAAGAATGGTGAGTCTGCACTCAGCATCTTCGACCGCAACACCTGTCACC
TTTACATGAACCCGTCTGGCAAGTTCATCATTGGTGGACCACAAGGCGATGCTGGGCTCACAGGACGCAAAATTATCATCGACACCTACGGTGGTTGGGG
AGCTCATGGTGGTGGTGCTTTCTCTGGCAAAGACCCGACCAAGGTGGACAGGTCAGCTGCATACATCACCAGGCAGATTGCCAAGTCGATTGTGGCGTCC
AAGTTGGCCAAGCGGGCCCTGGTGCAGCTGTCATATGCCATCGGGGTGGCCAAGCCCCTCTCCATCTTCGTGGAGACATATGGCTCCGAGCAGGGTGCCC
TGACTGCCGATGACGTTACGGACATTGTGAAGCTGGCATTCGACTGCCGCCCCGGAGCCATCGCCAAGTCCCTGCAGCTGACTGAGCCCAAGTACACTGA
GACGGCCGCATACTGCCACTTTGGCCGTGAGCCCCGCACCGAGAATGGCATCAAGTTCTTCGAGTGGGAGAACCCCGTTGACTTGTCCAAG
>Strombomonas accuminata methionine adenosyltransferase (MATX) amino acid translation, partial protein
EFLFSSESVNEGHPDKLCDQVSDSVLDACLAQDPKSKVACETAVKDNMVMVLGEITTQAKLDYEAIVRKAVRNIGFDSFVDDLDSVDSKGLNCDDCEVLVRINKQ
SPDIAGGVHVGRDEMDLGAGDQGIMFGYASDETKSTMPLTHYLATRLGKTLTEVRKDGTLWWLRPDGKTQVTIKYKQCADGSVEPLQIHSVLISTQHVEPGKRK
KKEEIRGYQGADGDEVAPTMKEMNEHLIEHVVIRTLKGITLKNGESALSIFDRNTCHLYMNPSGKFIIGGPQGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKV
DRSAAYITRQIAKSIVASKLAKRALVQLSYAIGVAKPLSIFVETYGSEQGALTADDVTDIVKLAFDCRPGAIAKSLQLTEPKYTETAAYCHFGREPRTENGIKFFEWENP
VDLSK
>Petalomonas cantuscygni methionine adenosyltransferase (MAT) mRNA, partial CDS
TTCATCTTCTCGTCCGAGCATGTGACCGAGGGTCACCCGGACAAGATGGCCGACCAGGTGTCGGACCGCATCCTTGATGCGTGCCTCGCCGTCGACCCCCT
TGCCAAGGTTGCCTGTGAGTCGGCACTGAAGACCGGTATGGTCATGGTCTTCGGTGAGATCACGACGAAGGCGCACATCGACTACCAGGAGCTCATCCGC
GCCACCGTCAAGGACATCGGCTTCGATGACTCGGAGAAGGGGTTCGACTACAAGACGTGCAACGTTCTTGTCGCCATCGAGCAGCAGTCCCCGGAGATCG
GCATGTCCGTTCACGGTGATGACGCTAAGTCGCACAGCGATGACCTTGGTGCTGGTGATCAGGGCCTCATGTTCGGCTATGCGACCAACGAGGACGCTGA
GACCCTCATGCCGGTTTCCTATGTACTCGCGCGCAACCTCGCGCAGCGCCTCTCCCAGGTGCGCAACGACAAGACCTGCGCGTGGGTGCGCCCGGATGGC
AAGACCCAGGTCTCTGTCGAGTACCAGGAGACGAAGGACGCCAACGGCCGGACGAAGCTCACCCCCGTTCGCGTGCACACCATCCTCATCTCCACCCAGC
ACGCGCCGAGCATCTCCCTGGACGAGATCCGCGCTGAGCTCACGCAGCACGTGATCAAGCCGGTCATCCCGGCCCACCTCCTGGACGACAAGGTCATCCTC
CACTTGAACCCCTCCGGGTCCTTCGTCGTCGGTGGCCCCAAGGGCGATGCCGGGCTCACTGGCCGCAAGATCATTGTTGACACCTACGGCGGCTGGGGCG
CCCACGGCGGAGGCGCCTTCTCGGGCAAGGACCCCACGAAGGTGGATCGTTCCGCGGCCTACGCGGCCCGCTGGGTCGCGAAGTCGCTCGTGGCCGCGG
GCTTCGCGGACCGCGCCCTTGTCCAGGTCGCGTACGCTATTGGGCTGGCGGCCCCCCTCTCGATCCACGTCGAGTCTTATGGCACGGGCAAGGTCCCGGAT
GCCGTCCTCACGGAGATCGTGAAGCACAACTTCGACCTGCGCCCGCAGCGCATCATTAAGGATCTGGACCTCCGCCGGCCGATCTACTACAAGACGGCGA
AGTTCGGCCACTTCGGCCGCACGGACCCCGACTTCACCTGGGAGCAGCCCAAGACCCTCACCCTC
>Petalomonas cantuscygni methionine adenosyltransferase (MAT) amino acid translation, partial protein
FIFSSEHVTEGHPDKMADQVSDRILDACLAVDPLAKVACESALKTGMVMVFGEITTKAHIDYQELIRATVKDIGFDDSEKGFDYKTCNVLVAIEQQSPEIGMSVHG
DDAKSHSDDLGAGDQGLMFGYATNEDAETLMPVSYVLARNLAQRLSQVRNDKTCAWVRPDGKTQVSVEYQETKDANGRTKLTPVRVHTILISTQHAPSISLDEI
RAELTQHVIKPVIPAHLLDDKVILHLNPSGSFVVGGPKGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPTKVDRSAAYAARWVAKSLVAAGFADRALVQVAYAI
GLAAPLSIHVESYGTGKVPDAVLTEIVKHNFDLRPQRIIKDLDLRRPIYYKTAKFGHFGRTDPDFTWEQPKTLTL
>Phacus orbicularis methionine adenosyltransferase (MAT) mRNA, partial CDS
TCCGAGTCCGTGAACGAGGGCCACCCCGACAAGCTGTGCGACCAGGTGTCTGATGCCGTCCTGGATGCCTGCATCCGCGAGGACCCCACCTCCCGTGTGG
CTTGCGAGACCTGCACCAAGACTGGTATGGTGATGATCTTCGGTGAGATCACCACCGCTGCCACCGTCAACTACGAGCAGGTGATCCGCGAGGCTCTGAA
GGAGATTGGCTACGATGACGTGAACAAGGGTCTGGACTACAGAACCTGCAACGTGATCGTCGCCATCGAGGAGCAGTCTCCCGATATCGCCCAGTCCGTC
GATGCCACCAAGCTGGAGGACATCGGAGCTGGTGACCAGGGCATCATGTTCGGCTACGCCACTGATGAGACCGAGTCTTACATGCCCCTGACCCACAACC
TGGCCACCTCTCTGGGCGCCCGCCTGACTGAGGTGCGCAAGACCGGTGTCTGCCCCTGGGTGCGCCCCGACGGCAAGACCCAGGTGACCTGCGAGTACCG
CCTGGACAATGGCCGCCCCATCCCCACCCGCGTGCACACCATCGTCATCTCCACCCAGCACTCCGAGGACATCACCCAGGACGAGATCAAGGCCCAGCTGA
TGGAGCACGTGATCAAGCCCGTGGTGCCCGCCGAGTACCTGGACGAGAAGACCGTGTACCACCTGAACCCCTCTGGCCGCTTCGTCATTGGTGGCCCTCAC
GGTGATGCCGGTCTGACTGGCCGCAAGATCATCATCGACACCTACGGTGGTTGGGGTGCCCACGGTGGTGGAGCCTTCTCCGGCAAGGACACCACCAAGG
TGGACCGCTCCGCTGCCTACGCCGCCCGTTGGGTTGCCAAGTCCCTGGTGGCTGCCGGCCTGGCCCACAGAGTCCTGGTGCAGCTGTCCTACGCCATCGGT
GTCTCCTACCCCCTGTCCGTGTTCGTCGACTCCTACGGCACCGGCATCACCCGCTCCGGCAAGACCGATGCCCAGCTGACCGAGATCGTCAAGAACAACTTC
GACCTGCGCCCCGGAGGAATCATCCGCGACCTGAACCTGCGCAGACCCGTCCTGAAGAAGACCGCCGCCTACGGCCACTTCGGACGCAACGACCCCGACT
TCACCTGGGAGACCCCCAAGCCCCTGACCCTG
>Phacus orbicularis methionine adenosyltransferase (MAT) amino acid translation, partial protein
SESVNEGHPDKLCDQVSDAVLDACIREDPTSRVACETCTKTGMVMIFGEITTAATVNYEQVIREALKEIGYDDVNKGLDYRTCNVIVAIEEQSPDIAQSVDATKLED
IGAGDQGIMFGYATDETESYMPLTHNLATSLGARLTEVRKTGVCPWVRPDGKTQVTCEYRLDNGRPIPTRVHTIVISTQHSEDITQDEIKAQLMEHVIKPVVPAEY
LDEKTVYHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDTTKVDRSAAYAARWVAKSLVAAGLAHRVLVQLSYAIGVSYPLSVFVDSYGTGIT
RSGKTDAQLTEIVKNNFDLRPGGIIRDLNLRRPVLKKTAAYGHFGRNDPDFTWETPKPLTL
Species tree topologies used for topology tests
MATX data set
SSU rRNA tree
(Thalassionema_nitzschioides,Asterionella_glacialis,(Cylindrotheca_closterium,((Ditylum_brightwellii,(Thalassiosira_pseudonana,(Detonula
_confervacea,Skeletonema_costatum))),(Aureococcus_anophagefferens,((Heterocapsa_triquetra,(Karlodinium_veneficum,(Karenia_brevis,
(Amphidinium_carterae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),((Lactuca_serriola,((((Phacus_inflexus,Phacus_orbicularis),(L
epocinclis_playfairiana,Lepocinclis_tripteris)),(Euglena_proxima,(((Euglena_viridis,Euglena_stellata),(Euglena_clara,(Euglena_gracilis,Euglen
a_hiemalis))),((Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_parapyrum,Monomorphina_pyrum))),((Trachelomonas
_sp,Trachelomonas_volvocina),(Strombomonas_accuminata,Trachelomonas_ellipsoidalis)))))),(Eutreptia_viridis,(Eutreptiella_gymnastica,E
utreptiella_braarudii)))),(Dendroctonus_frontalis,(Prymnesium_parvum,(Emiliania_huxleyi,Isochrysis_galbana)))))))));
Manually constructed tree
(Thalassionema_nitzschioides,Asterionella_glacialis,(Cylindrotheca_closterium,((Ditylum_brightwellii,(Thalassiosira_pseudonana,(Detonula
_confervacea,Skeletonema_costatum))),(Aureococcus_anophagefferens,((Heterocapsa_triquetra,(Karlodinium_veneficum,(Karenia_brevis,
(Amphidinium_carterae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),((Lactuca_serriola,(Dendroctonus_frontalis,((((Phacus_infle
xus,Phacus_orbicularis),(Lepocinclis_playfairiana,Lepocinclis_tripteris)),(Euglena_proxima,(((Euglena_viridis,Euglena_stellata),(Euglena_clar
a,(Euglena_gracilis,Euglena_hiemalis))),((Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_parapyrum,Monomorphina_
pyrum))),(((Trachelomonas_sp,Trachelomonas_volvocina),Trachelomonas_ellipsoidalis),Strombomonas_accuminata))))),(Eutreptia_viridis,(
Eutreptiella_gymnastica,Eutreptiella_braarudii))))),(Prymnesium_parvum,(Emiliania_huxleyi,Isochrysis_galbana))))))));
MATX data set excluding Aureococcus_anophagefferenss, Prymnesium, Lactuca and Dendroctonus
SSU rRNA tree
(Thalassionema_nitzschioides,Asterionella_glacialis,(Cylindrotheca_closterium,(((Heterocapsa_triquetra,(Karlodinium_veneficum,(Karenia_
brevis,(Amphidinium_carterae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),(((((Phacus_inflexus,Phacus_orbicularis),(Lepocinclis_
tripteris,Lepocinclis_playfairiana)),(Euglena_proxima,(((Strombomonas_accuminata,(Trachelomonas_ellipsoidalis,(Trachelomonas_volvocin
a,Trachelomonas_sp))),(Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_pyrum,Monomorphina_parapyrum)))),(Eugle
na_clara,((Euglena_gracilis,Euglena_hiemalis),(Euglena_stellata,Euglena_viridis)))))),(Eutreptia_viridis,(Eutreptiella_gymnastica,Eutreptiella
_braarudii))),(Isochrysis_galbana,Emiliania_huxleyi))),(Ditylum_brightwellii,(Thalassiosira_pseudonana,(Detonula_confervacea,Skeletonem
a_costatum))))));
Manually constructed tree
(Thalassionema_nitzschioides,Asterionella_glacialis,(Cylindrotheca_closterium,(((Heterocapsa_triquetra,(Karlodinium_veneficum,(Karenia_
brevis,(Amphidinium_carterae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),(((((Phacus_inflexus,Phacus_orbicularis),(Lepocinclis_
tripteris,Lepocinclis_playfairiana)),(Euglena_proxima,(((Strombomonas_accuminata,(Trachelomonas_ellipsoidalis,(Trachelomonas_volvocin
a,Trachelomonas_sp))),(Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_pyrum,Monomorphina_parapyrum)))),((Eugle
na_clara,(Euglena_gracilis,Euglena_hiemalis)),(Euglena_stellata,Euglena_viridis))))),(Eutreptia_viridis,(Eutreptiella_gymnastica,Eutreptiella_
braarudii))),(Isochrysis_galbana,Emiliania_huxleyi))),(Ditylum_brightwellii,(Thalassiosira_pseudonana,(Detonula_confervacea,Skeletonema
_costatum))))));
Rooted MATX data set
SSU rRNA tree
(Thalassionema_nitzschioides,Asterionella_glacialis,(Cylindrotheca_closterium,((Aureococcus_anophagefferens,(((Heterocapsa_triquetra,(
Karlodinium_veneficum,(Karenia_brevis,(Amphidinium_carterae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),(Dendroctonus_fro
ntalis,Lactuca_serriola)),((Prymnesium_parvum,(Isochrysis_galbana,Emiliania_huxleyi)),((TricVagi,EscherichK),((Euglena_proxima,(((Lepocin
clis_playfairiana,Lepocinclis_tripteris),(Phacus_orbicularis,Phacus_inflexus)),(((Trachelomonas_ellipsoidalis,Strombomonas_accuminata),(T
rachelomonas_sp,Trachelomonas_volvocina)),(((Euglena_stellata,Euglena_viridis),(Euglena_clara,(Euglena_gracilis,Euglena_hiemalis))),(Eu
glenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_parapyrum,Monomorphina_pyrum))))))),(Eutreptia_viridis,(Eutreptiella_
braarudii,Eutreptiella_gymnastica))))))),(Ditylum_brightwellii,(Thalassiosira_pseudonanai,(Detonula_confervacea,Skeletonema_costatum)))
)));
Manually constructed tree
((TricVagi,EscherichK),(((((Lepocinclis_playfairiana,Lepocinclis_tripteris),(Phacus_orbicularis,Phacus_inflexus)),(Euglena_proxima,((Strombo
monas_accuminata,(Trachelomonas_ellipsoidalis,(Trachelomonas_sp,Trachelomonas_volvocina))),(((Euglena_stellata,Euglena_viridis),(Eugl
ena_clara,(Euglena_gracilis,Euglena_hiemalis))),(Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_parapyrum,Monomo
rphina_pyrum))))))),(Eutreptia_viridis,(Eutreptiella_braarudii,Eutreptiella_gymnastica))),Dendroctonus_frontalis,(Lactuca_serriola,((Prymne
sium_parvum,(Isochrysis_galbana,Emiliania_huxleyi)),((Heterocapsa_triquetra,(Karlodinium_veneficum,(Karenia_brevis,(Amphidinium_car
terae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),(Aureococcus_anophagefferens,((Ditylum_brightwellii,(Thalassiosira_pseudon
ana,(Detonula_confervacea,Skeletonema_costatum))),(Cylindrotheca_closterium,(Asterionella_glacialis,Thalassionema_nitzschioides))))))))
);
Rooted MATX data set excluding Aureococcus_anophagefferenss, Prymnesium, Lactuca and Dendroctonus
SSU rRNA tree
(Thalassionema_nitzschioides,Asterionella_glacialis,(Cylindrotheca_closterium,(((Heterocapsa_triquetra,(Karlodinium_veneficum,(Karenia_
brevis,(Amphidinium_carterae,(Alexandrium_catenella,Lingulodinium_polyedrum))))),((Isochrysis_galbana,Emiliania_huxleyi),((TricVagi,Esc
herichK),((Euglena_proxima,(((Phacus_inflexus,Phacus_orbicularis),(Lepocinclis_tripteris,Lepocinclis_playfairiana)),((((Euglena_stellata,Eugl
ena_viridis),(Euglena_clara,(Euglena_gracilis,Euglena_hiemalis))),(Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_par
apyrum,Monomorphina_pyrum)))),((Strombomonas_accuminata,Trachelomonas_ellipsoidalis),(Trachelomonas_volvocina,Trachelomonas_
sp))))),(Eutreptia_viridis,(Eutreptiella_gymnastica,Eutreptiella_braarudii)))))),(Ditylum_brightwellii,(Thalassiosira_pseudonanai,(Skeletonem
a_costatum,Detonula_confervacea))))));
Manually constructed tree
((TricVagi,EscherichK),(((((Lepocinclis_playfairiana,Lepocinclis_tripteris),(Phacus_orbicularis,Phacus_inflexus)),(Euglena_proxima,((Strombo
monas_accuminata,(Trachelomonas_ellipsoidalis,(Trachelomonas_sp,Trachelomonas_volvocina))),(((Euglena_stellata,Euglena_viridis),(Eugl
ena_clara,(Euglena_gracilis,Euglena_hiemalis))),(Euglenaria_anabena,(Monomorphina_aenigmatica,(Monomorphina_parapyrum,Monomo
rphina_pyrum))))))),(Eutreptia_viridis,(Eutreptiella_braarudii,Eutreptiella_gymnastica))),((Isochrysis_galbana,Emiliania_huxleyi),((Heteroca
psa_triquetra,(Karlodinium_veneficum,(Karenia_brevis,(Amphidinium_carterae,(Lingulodinium_polyedrum,Alexandrium_catenella))))),((Dit
ylum_brightwellii,(Thalassiosira_pseudonana,(Detonula_confervacea,Skeletonema_costatum))),(Cylindrotheca_closterium,(Asterionella_gl
acialis,Thalassionema_nitzschioides)))))));
MATX data set of euglenids
SSU rRNA tree
(Monomorphina_pyrum,Monomorphina_parapyrum,(Monomorphina_aenigmatica,(Euglenaria_anabena,((Strombomonas_accuminata,(Tr
achelomonas_ellipsoidalis,(Trachelomonas_sp,Trachelomonas_volvocina))),(((Euglena_stellata,Euglena_viridis),(Euglena_clara,(Euglena_gr
acilis,Euglena_hiemalis))),(((Lepocinclis_playfairiana,Lepocinclis_tripteris),(Phacus_inflexus,Phacus_orbicularis)),(Euglena_proxima,(Eutrept
ia_viridis,(Eutreptiella_braarudii,Eutreptiella_gymnastica)))))))));
Manually constructed tree
(Monomorphina_pyrum,Monomorphina_parapyrum,(Monomorphina_aenigmatica,(Euglenaria_anabena,((Strombomonas_accuminata,(Tr
achelomonas_ellipsoidalis,(Trachelomonas_sp,Trachelomonas_volvocina))),(((Euglena_stellata,Euglena_viridis),(Euglena_clara,(Euglena_gr
acilis,Euglena_hiemalis))),(Euglena_proxima,(((Lepocinclis_playfairiana,Lepocinclis_tripteris),(Phacus_inflexus,Phacus_orbicularis)),(Eutrept
ia_viridis,(Eutreptiella_braarudii,Eutreptiella_gymnastica)))))))));