Twenty Third Annual Biotechnology Training Retreat March 22nd, 2014 CHRISTIAN BROTHERS RETREAT CENTER, NAPA VALLEY Attendee: Status (Faculty, Student, Industry): Riding Bus Y/N: Dept./Company: E-mail: Phone: Lab (if applicable): Title of Talk (if applicable): (AV provided at Retreat) Title of Poster (if applicable): Poster Easel Size Required (if applicable): Large (40x60)____________ Small (30x40)____________ Food Allergies: Do you, or anyone in your group, have a food allergy? Yes______ No ______ If answered “Yes” to the above question, please list allergy(ies) below as lunch will be provided at noon and the chef must know in advance of any special considerations for a guest. Please be aware that break refreshments will not be served with any dietary restrictions so participants with food allergies need to be aware of this risk. If you have food allergies, please provide your own snack items. Name: _____________________________________ Email: ________________________________ I have a food allergy to: ______________________________________________________________ I will bring an EpiPen or other such medication in the event that I have a reaction to an allergen. Yes _______ No _________ Biotechnology Program, One Shields Avenue, UC Davis, Tel: 530-752-3260, Fax: 530 752-4125 e-mail: biotechprogram@ucdavis.edu web: www.biotech.ucdavis.edu The following individuals in my group plan to attend: Name Status (Company scientist, faculty, student, postdoc, etc.) Name: Email: ☐Faculty Name: Email: ☐Faculty Name: Email: ☐Faculty Name: Email: ☐Faculty Name: Email: ☐Faculty ☐Student ☐Student ☐Student ☐Student ☐Student ☐PostDoc ☐PostDoc ☐PostDoc ☐PostDoc ☐PostDoc Will present poster? Will travel by UCD Bus? Yes No Yes No Yes No Yes No Yes No Yes No Yes No Yes No Yes No Yes No ☐Industry ☐Industry ☐Industry ☐Industry ☐Industry Return REGISTRATION FORM by March 1, 2014 and ABSTRACTS/PRESENTATIONS by March 8, 2014 to: Jacki Balderama, Event Manager, Biotechnology Program University of California, Davis CA, 95616 Tel: 530-752-8183 Fax 530-752-4125 jbalderama@ucdavis.edu Fee (per person): $45 (UC students*, post docs, staff, & community college affiliates) $55 UC Faculty $65 (all others) Make Payable To: Regents of Univ. of Calif. Or supply recharge #: ___________ *No fee for fellows currently receiving fellowships administered through the Biotechnology Program (NIH biotech fellows & Biotechnology fellows). Biotechnology Program, One Shields Avenue, UC Davis, Tel: 530-752-3260, Fax: 530 752-4125 e-mail: biotechprogram@ucdavis.edu web: www.biotech.ucdavis.edu Twenty Third Annual Biotechnology Training Retreat March 22, 2014 Christian Brothers Retreat Center Request for abstracts Due March 1, 2014 Please submit (1) e-mail attachment of electronic abstract, (2) registration form, and (3) this form filled out to: Jacki Balderama, jbalderama@ucdavis.edu . Authors: Title of Abstract: This abstract is for an oral presentation (Current trainees, trainers and companies only) for a poster presentation (All participants) for both oral and poster presentation (Current trainees, trainers and companies only) Poster Presentation: Please check easel size required (if applicable): Large (40x60)____________ Small (30x40)____________ Stand-alone easels with white boards (small boards: approx. 32"x40,” large boards approx. 40” x60”) will be available for mounting of your poster presentations. Push pins will also be supplied for your assistance. Abstract: Margins at top and sides to be 1", 4" at bottom. Use left justification only. A sample abstract is enclosed. Please use "Times" font, 12 point if it is available to you, except as may be necessary for special symbols. In any case, do not use less than 12 point font. Use upper case letters for the title, except where a lower case letter is the convention, e.g. "tRNA." The title is to be no more than 3 lines long. Skip a line and type the authors' names in bold, marking the speaker or presenter with an asterisk, *. On the next line type the department and institutional affiliation. Street and city are not required. Skip a line and begin typing the body of the abstract at the left margin. Abstract should give the rationale for the work, indication of methods, results and conclusions. References are optional. If references are used, they are to be cited in the text by superscript numbers: 1, 2, 3... Skip a line before typing reference 1, beginning with "1. ..." (i.e., not superscript). References should include title and inclusive pagination, with a colon between the volume number and the number of the first page. Do not skip a line between references. Electronic submission only. Microsoft Word is the preferred format. If you wish to submit an abstract but cannot supply a file in Word, please contact Jacki Balderama by email at jbalderama@ucdavis.edu or by phone at 530-752-1048. Biotechnology Program, One Shields Avenue, UC Davis, Tel: 530-752-3260, Fax: 530 752-4125 e-mail: biotechprogram@ucdavis.edu web: www.biotech.ucdavis.edu EXAMPLE Twenty Third Annual Biotechnology Training Retreat March 22, 2014 Christian Brothers Retreat Center Request for abstracts Due March 8, 2014 Please submit (1) e-mail attachment, (2) abstract, (3) this form, to the Biotechnology Program, University of California, Davis Authors: Manuel Torres, Marta Matvienko and John Yoder Title of abstract: CLUSTER ANALYSIS OF MULTI-DIMENSIONAL EXPRESSION DATA This abstract is _X for an oral presentation (Current trainees, trainers and companies only) ___ for a poster presentation (All participants) ___ for both oral and poster presentation (Current trainees, trainers and companies only) Biotechnology Program, One Shields Avenue, UC Davis, Tel: 530-752-3260, Fax: 530 752-4125 e-mail: biotechprogram@ucdavis.edu web: www.biotech.ucdavis.edu ABSTRACT EXAMPLE-1 CLUSTER ANALYSIS OF MULTI-DIMENSIONAL EXPRESSION DATA Manuel Torres*, Marta Matvienko, and John I. Yoder Department of Vegetable Crops, University of California, Davis, CA, 95616 We are interested in characterizing the signal, regulatory and developmental pathways of haustorium formation in parasitic plants. The defining feature of parasitic plants is their ability to form haustoria in response to host-plant recognition signals secreted in the rhizosphere. This parasite-specific organ attaches and invades host-plant tissue and transports fluid nutrients via a vascular connection. We have developed an in-vitro system that allows the identification of plant transcriptional responses during early haustorium development in parasitic and non-parasitic Scrophulariaceae. A phenolic derivative DMBQ (2,6 dimethoxybenzoquinone) present in plant-root exudates and a maize root exudate known to activate haustoria formation were used as haustorial inducers. We have isolated cDNAs of differentially abundant transcripts using PCR based subtraction hybridization. We assigned putative functions to 141 of these Early DMBQ Induced (EDI) transcripts using BLAST homology searches. These EDI transcripts were arrayed on nylon membranes and interrogated with normalized labeled gene–specific probes. Ninety-five percent of EDI transcripts induced by DMBQ were also induced by maize-root exudates. Only 6% of the EDI transcripts are induced in the non-parasite Lindenbergia after DMBQ exposure. Fifty percent of EDI transcripts are reduced in abundance in DMBQ exposed Lindenbergia. We are interested in differences in transcript abundance between these two closely related species and in identifying clusters of genes with similar expression patterns. These clusters of expressed transcripts are likely involved in recognition and transduction of host-root signals or in early morphological changes associated with haustorium formation in parasitic plants. One of our goals is to develop an image-analysis software application or to modify an existing one for analyzing gene expression profiles based on hybridization characteristics of multiple cDNA arrays. Biotechnology Program, One Shields Avenue, UC Davis, Tel: 530-752-3260, Fax: 530 752-4125 e-mail: biotechprogram@ucdavis.edu web: www.biotech.ucdavis.edu