“Best Practices in Mucosal Sampling Workshop” Sponsored by NIAID/NIH Image courtesy Otto Yang, Maria Teresa Ochoa, Gloria Preza, and Peter Anton Thursday, May 1, 2014 Masur Auditorium, NIH campus Day 1: Wednesday 4/30/14 MIG Program Meeting Invitation only Open to the public Day 2: Thursday: 5/1/14 8:00 – 8:30am On-Site Registration 8:30 – 8:45am Introduction and MIG PLOS collection launch: Florian Hladik and Thandi Onami 8:45 – 10:00am Collecting clinical and demographic data to evaluate mucosal samples. Chair, Alan Landay 8:45 – 9:10am Immunological changes during the menstrual cycle: Charles Wira 9:10 – 9:35am STI testing and treatment-influences on mucosal sampling: Susan Cu-Uvin 9:35 – 10:00am Launch of Enterprise Mucosal Sampling Guide: Patricia D’Souza 10:00 – 10:20am Coffee break- On Your Own 10:20 – 12:30pm Standardizing cervico-vaginal mucosal specimen analysis. Chair, Otto Yang 10:20 – 10:40am Cervico-vaginal mucosal sampling-- cytobrushes vs. vaginal biopsies: Florian Hladik Conference attendees and/or presenters are responsible for meals and/or light refreshments on their own and at their own cost. Government staff and/or Government contractors may not be involved in the provision or facilitation of food and/or light refreshments for conference attendees and/or presenters. 10:40 – 11:00am Using soluble immune mediators to study the mucosal milieu: Charlene Dezutti 11:00 – 11:20am Monitoring mucosal humoral responses following vaccination: Georgia Tomaras 11:20 – 11:40am Mucosal T cells and HIV susceptibility: Rupert Kaul 11:40 – 12:00pm Proteomic methods for monitoring mucosal responses: Blake Ball 12:00 – 12:30 Panel discussion 12:30 – 1:20pm Lunch – On Your Own 1:20 – 3:00pm Standardizing gastrointestinal mucosal specimen analysis. Chair, Phil Bergin 1:20 – 1:40pm GI mucosal sampling: options for protocol feasibility and participant compliance Peter Anton 1:40 – 2:00pm Monitoring mucosal T cell responses to vaccines: Steve DeRosa 2:00 – 2:20pm Early initiation of ART preserves Th17 cells and reverses HIV-related immune activation: Alexandra Schuetz 2:20 – 2:45pm Mixing OMICS to monitor mucosal inflammation: Rafick Sekaly/Mark Cameron 2:45 – 3:00pm Coffee break – On Your Own 3:00 – 4:30pm Novel methods to interrogate mucosal samples: Chair, Jean Charles Grivel 3:00 - 3:20pm Nanowell approaches to measuring mucosal immune responses: Chris Love 3:20- 3:40pm FcR bearing effector cells in the human female genital tract: Rena Astronomo 3:40 - 4:00pm Optimizing cryopreserved functional responses: Barbara Shacklett 4:00 – 4:30pm Discussion and wrap-up Conference attendees and/or presenters are responsible for meals and/or light refreshments on their own and at their own cost. Government staff and/or Government contractors may not be involved in the provision or facilitation of food and/or light refreshments for conference attendees and/or presenters.