Supplemental Figure 1: Antibody specificity for Hnf4 (C-19). ChIP-qPCR
analysis was done on WT and Hnf4-HNull mouse livers. DNA fragments
immunoprecipitated with Hnf4 antibody were PCR amplified using primers
designed against known Hnf4 binding regions within Apoc3, Baat, and Apoa1,
and a negative control region. Data are reported at fold enrichment (y-axis) of
Hnf4binding in Hnf4-HNull (black bar) mouse liver compared to WT (white
bar) mouse liver. ‘*’ p < 0.05. As expected, binding of Hnf4 to Apoc3, Baat, and
Apoa1 (detected by C-19 anti-HNF4 antibody) dramatically decreased in Hnf4HNull mouse livers when compared to WT controls, but does not change at
negative control region. Thereby validating specificity of Hnf4 antibody used for
ChIP-qPCR analysis.