表一、海生所 103 學年專題討論(二) 摘要表
IMB 103 Seminar (II) Abstract form
報告日期/Date：2015 年/year 03 月/month 25 日/day
班別/學生姓名：
Class/Name: 碩士二級/馮偉超
題目 Title：Characterization of two splice variants of human organic anion
transporting polypeptide 3A1 isolated from human brain
作者 Author(s): Robert D. Huber, Bo Gao, Marguerite-Anne Sidler Pfa¨ndler,
Wenting Zhang-Fu, Simone Leuthold, Bruno Hagenbuch, Gerd Folkers, Peter
J. Meier, and Bruno Stieger
期刊 Journal name: Am J Physiol Cell Physiol
期頁數 Issue and page nos: 292: C795–C806, 2007
摘要 Abstract:
Characterization of two splice variants of human organic anion transporting
polypeptide 3A1 isolated from human brain. In the present study we isolated two
splice variants of organic anion transporting polypeptide 3A1 (OATP3A1_v1
and OATP3A1_v2) from human brain. OATP3A1_v2 lacks 18 amino acids (aa)
at the COOH-terminal end (692 aa) but is otherwise similar in sequence to
OATP3A1_v1 (710 aa). OATP3A1_v1 exhibits a wide tissue distribution, with
expression in testis, various brain regions, heart, lung, spleen, peripheral blood
leukocytes, and thyroid gland, whereas OATP3A1_v2 is predominantly
expressed in testis and brain. On the cellular and subcellular levels OATP3A1_v1
could be immunolocalized in testicular germ cells, the basolateral plasma
membrane of choroid plexus epithelial cells, and neuroglial cells of the gray
matter of human frontal cortex. Immunolocalization of OATP3A1_v2 included
Sertoli cells in testis, apical and/or subapical membranes in choroid plexus
epithelial cells, and neurons (cell bodies and axons) of the gray and white matter
of human frontal cortex. The rodent ortholog Oatp3a1 was also widely
distributed in rat brain, and its localization included somatoneurons as well as
astroglial cells.Transport studies in cRNA-injected Xenopus laevis oocytes and
in stably transfected Chinese hamster ovary FlpIn cells revealed a similar broad
substrate specificity for both splice variants. Transported substrates include
prostaglandin (PG)E1 and PGE2, thyroxine, and the cyclic oligopeptides BQ123 (endothelin receptor antagonist) and vasopressin. These studies provide
further evidence for the involvement of OATPs in oligopeptide transport. They
specifically suggest that OATP3A1 variants might be involved in the regulation
of extracellular vasopressin concentration in human brain and thus might
influence the neuromodulation of neurotransmission by cerebral neuropeptides