Add correlation coeeficient to this assignment (Ds to soil temp, air temp or humidity) Your values for X and Y should come in pairs. First of all calculate Xbar and Ybar: these are the means of the X's and the Y's respectively, so for Xbar, add up your values for X and divide by 8. Similarly, for Ybar, add up your values for Y and divide by 8. Next, we calculate Sx and Sy, the sample standard deviations of X and Y. To calcuate Sx, we proceed as follows: for each X value, calcuate (X - Xbar)^2 (the square of X - Xbar), add all these up and then divide by 7; taking the square root of your result yields Sx. The procedure for Sy is analagous. Now we come to the final hurdle. For each pair of X and Y-values, calculate (X - Xbar).(Y - Ybar) (ie. multiply (X - Xbar) by (Y - Ybar)), then add up the results you get for all the pairs, and then divide by 7.Sx.Sy, (ie. the product of Sx, Sy and (n-1) = 8 -1 = 7). This gives you the correlation coefficient. Positive correlation: If x and y have a strong positive linear correlation, r is close to +1. An r value of exactly +1 indicates a perfect positive fit. Positive values indicate a relationship between x and y variables such that as values for x increases, values for y also increase. Negative correlation: If x and y have a strong negative linear correlation, r is close to -1. An r value of exactly -1 indicates a perfect negative fit. Negative values indicate a relationship between x and y such that as values for x increase, values for y decrease. No correlation: If there is no linear correlation or a weak linear correlation, r is close to 0. A value near zero means that there is a random, nonlinear relationship between the two variables Note that r is a dimensionless quantity; that is, it does not depend on the units employed. A perfect correlation of ± 1 occurs only when the data points all lie exactly on a straight line. If r = +1, the slope of this line is positive. If r = -1, the slope of this line is negative. A correlation greater than 0.8 is generally described as strong, whereas a correlation less than 0.5 is generally described as weak. These values can vary based upon the "type" of data being examined. A study utilizing scientific data may require a stronger correlation than a study using social science data. Calculator: http://easycalculation.com/statistics/correlation.php Correlation Co-efficient : Correlation(r) =[ NΣXY - (ΣX)(ΣY) / Sqrt([NΣX2 - (ΣX)2][NΣY2 - (ΣY)2])] where N = Number of values or elements X = First Score Y = Second Score ΣXY = Sum of the product of first and Second Scores ΣX = Sum of First Scores ΣY = Sum of Second Scores ΣX2 = Sum of square First Scores ΣY2 = Sum of square Second Scores Correlation Co-efficient Example: To find the Correlation of X Values Y Values 60 3.1 61 3.6 62 3.8 63 4 65 4.1 Step 1: Count the number of values. N=5 Step 2: Find XY, X2, Y2 See the below table X Y Value Value 60 3.1 61 3.6 62 3.8 63 4 65 4.1 X*Y 60 * 3.1 = 186 61 * 3.6 = 219.6 62 * 3.8 = 235.6 X*X 60 * 60 = 3600 61 * 61 = 3721 62 * 62 = 3844 63 * 63 = 63 * 4 = 252 3969 65 * 4.1 = 65 * 65 = 266.5 4225 Y*Y 3.1 * 3.1 = 9.61 3.6 * 3.6 = 12.96 3.8 * 3.8 = 14.44 4 * 4 = 16 4.1 * 4.1 = 16.81 Step 3: Find ΣX, ΣY, ΣXY, ΣX2, ΣY2. ΣX = 311 ΣY = 18.6 ΣXY = 1159.7 ΣX2 = 19359 ΣY2 = 69.82 Step 4: Now, Substitute in the above formula given. Correlation(r) =[ NΣXY - (ΣX)(ΣY) / Sqrt([NΣX2 - (ΣX)2][NΣY2 (ΣY)2])] = ((5)*(1159.7)-(311)*(18.6))/sqrt([(5)*(19359)-(311)2]*[(5)*(69.82)(18.6)2]) = (5798.5 - 5784.6)/sqrt([96795 - 96721]*[349.1 - 345.96]) = 13.9/sqrt(74*3.14) = 13.9/sqrt(232.36) = 13.9/15.24336 = 0.9119 AP Environmental Science Wheeler High School Mr. Walstead Leaf Litter Biodiversity (adapted from Dr. Kim Largen’s at George Mason University EVPP 111 lab write up) ________________________________________________________ Background Species diversity is a characteristic that is unique to a community level of biological organization. The biodiversity in different communities has been severely affected by human activities. The number of species in a community is very important. There seems to be evidence that the greater the species diversity, the more stable the community. When diversity is low, the community is less stable. A community with low diversity is less able to rebound from severe disturbance such as pollution and habitat disruption. The purpose of this lab is to measure the biodiversity of organisms found in a sample of leaf litter collected on campus. A Berlese funnel apparatus will be used to separate from the leaf litter the small organisms dwelling within it. The organisms will then be organizing into similar taxonomic categories and counted. Finally, the species diversity of the leaf litter sample will be calculated. ________________________________________________________ Procedure Day 1 1. Collect a leaf litter sample as follows: a. on a patch of the forest floor, envision a square that is approximately 30cm on each side (roughly 1 square foot) b. from this area, collect all of the leaf litter to the top layer of soil-like material (down to a depth of approximately 1.5cm, to the extent that you can remove it with your fingers) and place into your plastic collection bag 2. Record the following information at the site: a. Soil Temperature b. Air Temperature c. Relative humidity d. Description of the litter (depth, type, dry/moist, etc) e. Description of the area (Light penetration/canopy, types of vegetation, land features, soil type, etc.) f. Sketch the area (from a birdseye view) 3. Return to the lab. Day 1 (cont) 4. Obtain a Berlese funnel apparatus. Place a piece of marking tape on the beaker and label it with your names. 5. Place a small volume of methanol in the bottom of the beaker 6. Place the funnel in the top of the plastic container and place the screen plug in the base of the funnel. 7. Place the leaf litter that you collected in the woods into the top of the funnel. As the leaf litter dries, from the top down, the organisms in the leaf litter will migrate downward (trying to stay in the moist litter) and will eventually fall into the methanol which will preserve them for later observation. Day 2 1. Discard the leaf litter from the funnel of your Berlese apparatus. PLEASE BE SURE THAT YOU DO NOT THROW AWAY THE SCREEN PLUG FROM THE BOTTOM OF THE FUNNEL!!!!!!!!!!! 2. Obtain a dissecting microscope 3. Pour the methanol from the bottom of the container, which now contains the organisms from the leaf litter, into one or more petri dishes (depending on how many in your group will be assisting with identification). 4. Observe the organisms in the petri dish under a microscope. a. Separate the sample into piles of like organisms within the petri dish. b. Determine the total number of each type of organism identified in your group's sample. Record this data in Table 1 in the column headed “absolute abundance” ________________________________________________________ Data Analysis 1. Determine the relative abundance of each individual species and record in Table 1 (you determined the absolute abundance of each species by counting the number of individuals of each different species). Relative abundance compares the number of organisms of a particular species with the total number of organisms found in the sample. Relative abundance of a species in a sample is calculated by dividing the number of individuals of that species by the total number of individuals in the entire sample. An example calculation follows: Relative abundance = ni / N ni = actual number of individuals of species i N = the total number of individuals of all types collected in sample Example data and calculations for relative abundance: 2. Determine the diversity of your sample using Simpson’s Index of Diversity and record in Table 1. The following example illustrates how to calculate Simpson's Index of Diversity: Ds = 1 - {50(49) + 25(24) + 10(9)} 85(84) Ds = 1 - (Σni(ni-1) / N(N-1)) Ds values closer to 0 = low diversity Ds = closer to 1 = greater diversity Ds = 1 - 3140 / 7140 Ds = 1 - 0.44 Ds = 0.56 Record your group's value for Simpson's Index of Diversity in Table 2 and on the transparency (or blackboard) Record other groups' values for Simpson's Index of Diversity and location information in your Table 2. ________________________________________________________ Sample Table 1 Sample Table 2 This is a formal lab report. Be sure you include the following: Introduction (10pts) – 1-2 Paragraphs discussing types of biodiversity, why we should care about it, and factors that influence it. (A hypothesis is not necessary) Materials and Method (5pts) – Describe the procedure in paragraph form. Results (10pts) – Site description and sketch 3 Scatter Plots and Correlation Properly labeled tables 1 & 2 Coefficients for (air temp. vs. Ds, Information summarized in a soil temp vs. Ds, & humidity vs. Ds) paragraph. Discussion (10pts) - 1-2 paragraphs that explain the “WHY" of your results. Is their a correlation of Ds to Air temp, soil temp. or humidity? References (5 pts) – Any source used in the introduction or to clarify an explanation in the discussion (minimum 2) Layout/Neatness (5pts) Conventions of writing (5 pts) **See the Formal Lab Report Guide for Complete Guidelines** Identifying Arthropods Major External Characteristics 1. Exoskeleton containing chitin 2. Body bilaterally symmetrical 3. Body segments grouped into specialized regions (= tagmata, plural) 4. Jointed appendages 5. These jointed appendages variously specialized for feeding, locomotion, sensing Key to the Adults of the Common Classes of Arthropoda 1a. Two pairs of antennae (one pair may be reduced, difficult to see); Number of legs variable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . Class Crustacea, go to 2 1b. One pair of antennae or none. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2a. Two distinct body regions (cephlothorax and abdomen); Five pairs of thoracic legs. . . . . . . . . . . . .. . . crayfish, lobsters, shrimp; Order Decapoda 2b. Three distinct body regions (head, thorax, abdomen); Seven pairs of thoracic legs . . . . . . . . . . . . sowbugs, pillbugs, roly-polys; Order Isopoda 3a. No antennae; Two distinct body regions (cephlothorax and abdomen); Four pairs of legs;. . . . . . . . . . . . . . . . . . spiders, ticks, scorpions, etc; Class Arachnida 3b. One pair of antennae. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 4a. Three distinct body regions (head, thorax, abdomen); Three pairs of thoracic legs. Wings present or absent. . . . . . . . . . . . . . Class Insecta 4b. Two distinct body regions (head and trunk). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 5a. One pair of legs per trunk segment. . . . . . . . . . . . . . . . . . . . . . centipedes; Class Chilopoda 5b. Two pairs of legs per trunk segment. . . . . . . . . . . . . . . . millipedes; Class Diplopoda Arachnida Isopoda Chilopoda Diplopoda Insecta