George R. Bousfield, PhD
Professor
Protein Core Laboratory Director
Office: 431 Hubbard Hall
Phone: (316) 978-6088
Fax: (316) 978-3772
Email:george.bousfield@wichita.edu
Education:
Institution and Location
Degree
Year
Field of Study
Saginaw Valley State College,University Center, MI B.S.
1974
Biology and Chemistry
Indiana University, Bloomington, IN
M.A.
1976
Zoology
Indiana University, Bloomington, IN
Ph.D.
Professional Experience:
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1978 to 1990: training in glycoprotein chemistry in the laboratory of Darrell N. Ward at
The University of Texas, M.D. Anderson Cancer Center, Houston, TX.
1991: Wichita State University, Department of Biological Sciences.
Research Interests
My laboratory is interested in the structure-function relations of the gonadotropins. These are
members of the glycoprotein hormone family which consists of the three gonadotropins,
luteinizing hormone (LH), follicle-stimulating hormone (FSH), and chorionic gonadotropin
(CG). The fourth member of this protein family is thyroid stimulating hormone (TSH). The
human and the horse are the two species from which both pituitary gonadotropins, LH and FSH,
as well as the placental gonadotropin, CG, have been isolated and characterized. The equine
gonadotropins are a particularly interesting group of gonadotropins because they are exceptions
to some of the general rules for gonadotropin structure-function relationships that were
established by experiments which employed human and other mammalian gonadotropins.
Research Projects
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Characterization of eCG isoforms
Characterization of eLH isoforms
Characterization of alpha subunit Asn-56 oligosaccharides
Characterization of eLH/CG beta O-glycosylation
Folding domains of alpha subunits
Glycosylation of hCG free-alpha subunit preparations
Expression of equine gonadotropins in a baculovirus expression system
Recent Publications
1. Butnev V.Y., Gotschall R.R., Baker V.L., Moore W.T., Bousfield G.R.: ""Negative
Influence of O–linked Oligosaccharides of High Molecular Weight Equine Chorionic
Gonadotropin (eCG) on its LH and FSH Receptor Binding Activities." Endocrinology
137: 2530-2542, 1996
2. Gotschall R.R. and Bousfield G.R.: "Oligosaccharide mapping reveals hormone–
spcecific glycosylation patterns on eqiune gonadotropin ?-subunit Asn56."
Endocrinology 137: 2543-2557, 1996
3. Butnev V.Y., Gotschall R.R., Baker V.L., Moore, W.T., Gout, P.W. and Bousfield .GR.:
Glycosylated equine prolactin and its carbohydrate moiety. J Protein Chem 15: 413-426,
1996
4. Bousfield GR, Butnev VY, Gotschall RR, Baker VL, Moore WT: Structural features of
mammalian gonadotropins. Mol. Cell. Endocrinol. 125: 3–19, 1996
5. Butnev VY, Gotschall RR, Butnev, VY, Baker VL, Moore WT, Bousfield GR: Inhibitory
influence of ?-subunit Asn56 oligosaccharide on in vitro subunit association and FSH
receptor binding of equine gonadotropins. Biol. Reprod. 58: 458–469, 1998
6. Bousfield, G.R. LH (Luteinizing Hormone), in Encyclopedia of Reproduction, Knobil, E
and Neill, JD, eds. Academic Press, San Diego,Vol. 2, 1034-1054, 1999
7. Bousfield, G.R., Baker, V.L., Gotschall, RR., Butnev, V.Y., and Butnev, V.Y.:
Carbohydrate Analysis of Glycoprotein Hormones. Methods. 21: 15-39, 2000
8. Bousfield, G.R., Butnev, V.Y. and Butnev, V.Y.: Identifcation of 12 O-glycosylation
sites in eLH and eCG ? subunits by solid-phase Edman degradation. Biol. Reprod. 64:
136-147, 2001
9. Walton, W.J., Nguyen, V.T., Butnev, V.Y., Singh, V., Moore, W.T., and Bousfield, G.R.:
Characterization of Human Follicle-Stimulating Hormone Isoforms Reveals a NonGlycosylated ?-subunit In Addition to the Conventional Glycosylated ?-subunit. J. Clin.
Endocrinol. Metab. 86:3675-3585, 2001.
10. Butnev, V.Y., Singh, V., Nguyen, V.T., and Bousfield, G.R.: Truncated eLH?,
Asparagine56-deglycosylated-eLH? Combine to Produce a Potent Follicle-Stimulating
Hormone Antagonist. J. Endocrinol. 172: 545-555, 2002
11. Nguyen VT, Singh V, Butnev VY, Gray CM, Westfall S, Davis JS, Dias JA, Bousfield
GR. Inositol Phosphate Stimulation by LH Requires the Entire ? Asn56 Oligosaccharide.
Molec. Cell. Endocrinol. 2003; 199: 73-86.
Courses Taught
Number
Course Name
Syllabus
BIOL 210 Introductory Biology I
BIOL 310 Human Reproduction - Issues and Perspectives
BIOL 640K Reproductive Biology
BIOL 640U Glycobiology
BIOL 767 Mechanisms of Hormone Action
Biol. 210. General Biology I. (4). 3R; 2L. General education introductory course. Introduces fundamental concepts
in cellular and molecular biology. Includes basic biological chemistry; cell and membrane structure and function;
aerobic and anaerobic respiratory pathways; intermediary metabolism and photosynthesis; regulation of cellular
activities at genetic and protein levels; cellular reproduction; mechanisms of inheritance at molecular, organismal
and population levels; phylogeny; and evolution. The laboratory develops student's skills in the experimental
method, basic laboratory procedures, and written communication of scientific information using topics related to the
lecture. Students may not receive credit for both Biol. 204 (no longer offered) and Biol. 210. Students wishing to
repeat Biol. 204 may enroll in this course, subject to the credit limitations indicated above. Prerequisite: concurrent
enrollment in Chem. 111 is recommended.