2015 ZEBRAFISH GENETICS AND DEVELOPMENT 2015 COURSE SCHEDULE
Sun 9 Aug
18:00 – 18:30
18:30 – 18:45
18:45 – 19:45
19:45 – 21:30
Light dinner – cold cuts & veggies (Loeb G70)
Course Welcome: Erez Raz and Corinne Houart (course directors)
Lecture: Overview of zebrafish fish development and genetics: Lila Solnica-Krezel
Poster Session & Beer
Mon 10 Aug
08:30 – 12:00
13:00 – 13:15
13:15 – 13:30
13:30 – 17:30
19:30 – 21:30
21:30 – 22:00
Pit Talk and Lab: Early zebrafish development: Lila Solnica-Krezel
IACUC training: Amy Hancock-Ronemus
Introduction to stereoscopes: Dave Raible
Development and embryo anatomy: Lila Solnica-Krezel and Dave Raible
Research Lectures and Discussion: Will Talbot and Lila Solnica-Krezel (Starr 209)
Lab: Overnight time lapse set up with Andres Collazo & Jim Fadool
Tue 11 Aug
08:30 – 12:30
13:30 – 14:30
14:30 – 17:00
17:00 – 18:00
19:30 – 21:30
21:30 – 22:00
Pit Talk and Lab: in vitro fertilization, sperm freezing, fin clipping: Cecilia Moens
Pit Talk: meiotic mapping, cloning mutations: Will Talbot and Ian Woods
Lab: Mapping with Bulk Segregant Analysis: Talbot/Woods
Review Lecture: Developmental Genetics: Mary Mullins
Research Lectures & Discussion: Alex Schier and Mary Mullins (Starr 209)
Lab: Overnight time lapse set up with Andres Collazo & Jim Fadool
Wed 12 Aug
08:30 – 11:30 Pit talk and lab: mRNA over-expression/mutant rescue: Mary Mullins
13:00 – 14:30 Pit Talk: genomics and web resources: Will Talbot and Ian Woods
14:30 – 16:00 Bioinformatics Lab: Will Talbot and Ian Woods
16:00 – 16:30 Wrap-up: In vitro fertilization
16:30 – 18:00 Round Table: Genome Editing in zebrafish Lila Solnica Krezel, Cecilia Moens, Sharon
Amacher
19:30 – 21:30 Research Lectures & Discussion: Sharon Amacher and Cecilia Moens (Starr 209)
21:30 - 22:30 Wrap-up mRNA rescue: Mary Mullins.
Thu 13 Aug
08:30 – 09:00 Pit Talk: Morpholino/ TALENS, CRISPR injections: Mary Mullins
09:00 – 10:45 Lab: Morpholino/ TALENS, CRISPR injections: Mary Mullins
10:45 - 11:45 Round table discussion: Morpholino limitations Mullins, Houart, Moens, Amacher
13:30 – 14:30 Lecture: Introduction to Microscopy: Andres Collazo
14:30 – 17:00 Hands-on introduction to microscopes & imaging: Andres Collazo, Dave Raible, Jim
Fadool, Sharon Amacher and Corinne Houart.
19:15 – 21:15 Research Lectures and Discussion: Erez Raz and Chris Beattie (Starr 209)
21:15 – 22:15 Wrap-up: morpholino and DNA injections: Mary Mullins
Fri 14 Aug
09:00 – 10:00 Round Table: Zebrafish husbandry, building a facility, breeding and raising fry
10:00 – 12:00 Round Table discussion: Aquatic Systems, Fish Facility Management: Corinne Houart,
Michael Granato (with suppliers).
13:00 – 14:30 Pit Talk: Timelapse imaging of germ cell migration: Erez Raz
14:30 – 18:00 Lab: Imaging germ cell migration: Erez Raz
19:00 – 20:00 Research Lecture and Discussion: Rolf Karlstrom (Starr 209)
20:00 – 23:00 Making movies and analysis of germ cell migration: Erez Raz
Sat 15 Aug
09:00 – 11:00 Wrap-up: student presentations: Erez Raz
11:00 – 12:00 Round table discussion: Zebrafish as a disease model; Chris Beattie
13:00 – 13:30 Pit Talk: RGC labelling and local gene expression by focal heat shock: Rolf Karlstrom
13:30 – 18:30 Lab: RGC labelling and local gene expression via focal heat shock: Rolf Karlstrom
19:00 – 23:30 Staff Party; Student Movie Night
Meal times at Swope: Breakfast: 07:00-08:30 Lunch: 11:30-13:00 Dinner: 17:00-19:00
Sun 16 Aug
(Falmouth Road Race)
Breakfast on your own.
13:00 – 15:00 Imaging of dye labelled RGCs and focal heat shocked embryos
15:00 – 16:15 Tour of the MRC: Caitlin Inglis & April Freeman
16:30 – 18:00 Student Faculty soccer challenge
18:30 – 21:00 Course BBQ
21:00 – 21:45 Lab: Overnight time lapse set up with Andres Collazo/Jim Fadool
Mon 17 Aug
08:30 – 09:00 Pit Talk: Immunostaining & in situ hybridization: Corinne Houart and Sharon Amacher
09:00 – 12:00 Lab: Mounting, analysis, and imaging of immunostained or in situ hybridized embryos:
Corinne Houart and and Sharon Amacher.
13:00 – 15:00 Lab: Continuation from morning
15:00 – 17:00 Lab: Making powerpoint presentations from immunostaining and in situ hybridization data
17:00 – 18:00 Wrap-up: student presentations with Corinne Houart and Sharon Amacher
20:00 – 21:15 Chi-Bin Chien lecture: Kelly Monk
Tue 18 Aug
09:00 – 11:00 Round Table Discussion: Transgenesis Dave Raible
11:00 – 12:00 Pit Talk: Chimeric Analysis: Cecilia Moens and Michael Granato
13:00 – 18:00 Lab: Chimeric analysis. Blastula transplants: Michael Granato, Jim Fadool, and Andres
Collazo. Germ Cell transplants & Gastrula transplants: Cecilia Moens.
19:30 – 21:30 Research Lectures and Discussion: Andres Collazo and Corinne Houart (G70)
21:30 – 22:15 Lab: Overnight time lapse set up with Andres Collazo/Jim Fadool
Wed 19 Aug
09:00 – 11:00 Imaging of chimeras and wrap-up of chimeric analysis: Cecilia Moens, Jim Fadool, Michael
Granato, and Andres Collazo
11:00 – 12:00 Pit Talk: Corinne Houart
13:00 – 18:00 Lab: Fate mapping, lineage analysis & transplants: Corinne Houart. Continue to take
pictures of blastula/gastrula transplants on compound and confocal scopes.
19:30 – 20:30 Research Lecture and Discussion: Dave Raible and Debbie Yelon (G70)
Thu 20 Aug
09:00- 11:00 Wrap up: Transplants, fate mapping and lineage analysis: Corinne Houart
11:00 – 12:00 Round Table discussion: Responsible Conduct of Research: Image Manipulation: Michael
Granato, Corinne Houart
13:00 – 14:00 Pit Talk: imaging subcellular structures: Dave Raible and Debbie Yelon
14:00 – 18:00 Demonstrations: imaging and labeling: Dave Raible and Debbie Yelon
19:30 – 20:30 Research Lectures and Discussion: Michael Granato and Steve Wilson (G70)
Fri 21 Aug
09:00 – 9:30 Pit Talk: Motor and visual behaviors: Michael Granato, Jim Fadool, Steve Wilson
9:30 –12:30 Lab: Motor and visual behaviors: Michael Granato, and Jim Fadool , Steve Wilson
13:30 – 16:00 Lab: continuation from morning: Motor and visual behaviors: Granato, Fadool, Wilson
16:00 – 17:00 Round Table Discussion: Imaging, Atlas and TG reporter resources: Corinne Houart
19:30 – 21:30 Research Lecture and Discussion: Jim Fadool and Ken Poss (G70)
Sat 22 Aug
09:00 – 10:00 Pit Talk: Adult dissection/biopsy and analysis of fin regeneration; Ken Poss
10:00 –12:00 Lab: Adult dissection/biopsy and analysis of fin regeneration Ken Poss
13:00 – 16:00 Lab: Adult dissection and Regeneration. Imaging of results: Ken Poss
17:00 – 18:00 Course Wrap-up: Corinne Houart, Sharon Amacher
18:00 – 21:00 Course Dinner sponsored by Pentair Aquatic Ecosystems and Techniplast (Swope Patio)
Meal times at Swope: Breakfast: 07:00-08:30 Lunch: 11:30-13:00 Dinner: 17:00-19:00