Mapping of biological data

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Service package 3: Mapping of biological data

The bioinformatics platform in integrated system biology (SYSBIO) offers three service packages:

SP1: Sequence assembly and annotation

SP2 : DNA sequence analysis

SP3: Mapping of biological data

In SP3 (mapping of biological data) SYSBIO offers to perform mapping of biological data such as expression data of genes from microarray studies to metabolic pathways. SYSBIO can also perform mapping of high-throughput sequence data (RNA-seq) to bacterial genomes or large

DNA fragments.

Mapping of gene expression data: Results can be used to view the expression of complete metabolic pathway rather than single genes.

Mapping of RNA-seq data: Results can be used to calculate differentially expressed genes (if samples from two or more conditions) and to improve gene annotation.

Table 1 Standard commissioning in SP3 mapping of biological data

Service

Package (SP) Task Specification

SP3.a

SP3.b

Mapping of gene expression data:

Mapping of RNA-seq data:

Sigma factors: If -10 and -35 binding motifs are known and conserved or organism closely related to e. coli , SYSBIO may perform prediction of different sigma factors.

Search matrices are built from known binding sites and genomes (or other DNA) are scanned for similar motifs.

Rho independent terminators: Genomes (or other DNA) are scanned for Rho independent terminators.

Platform of Integrated System Biology

University of Tromsø, N-9037 Tromsø, Telephone: (+47) 77 64 40 00, Fax (+47) 77 64 47 65

Direct telephone: (+47) 77 62 33 72, E-mail: sysbio@ik.uit.no

http://uit.no/sysbio

Preliminary order form Service package 3:

Mapping of biological data

.

Please place tick marks to indicate which tasks are desired. For simplicity this icon

 can be copied and replaced by the empty boxes. Then return this electronic form by E-mail to SYSBIO , and you will receive in response a tailored order form and prizes for placing the final order.

Order:

Date: …../…../20……

Title:

Customer:

Institution:

Address:

Contact person 1:

E-mail:

Phone:

Contact person 2:

E-mail:

Phone:

Order:

(Tick  )

SP Task

SP3.a Mapping of gene expression data

SP3.b Mapping of RNA-seq data

Platform of Integrated System Biology

University of Tromsø, N-9037 Tromsø, Telephone: (+47) 77 64 40 00, Fax (+47) 77 64 47 65

Direct telephone: (+47) 77 62 33 72, E-mail: sysbio@ik.uit.no

http://uit.no/sysbio

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Service package 3 (to be used for SP3.a and b)

Details Information

Project details:

Organism name:

Size of DNA:

Mapping of gene expression data:

Gene expression data:

Number of datasets:

…………………………………………………………

………………..kb

yes no

yes no

………………..datasets

Mapping of RNA-seq data:

Annotation update:

Differential expression

Number of datasets: a :

yes no

yes no

yes no

………………..datasets a If RNA-seq data from more than one conditions are available.

Platform of Integrated System Biology

University of Tromsø, N-9037 Tromsø, Telephone: (+47) 77 64 40 00, Fax (+47) 77 64 47 65

Direct telephone: (+47) 77 62 33 72, E-mail: sysbio@ik.uit.no

http://uit.no/sysbio

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