AOTGR1-42 Protocol for ‘Paired Paddocks’ in the DAFF project: ‘Farmers leading and learning about the soil carbon frontier’ The theory behind ‘paired paddocks’ as an extension tool, is to effectively communicate and demonstrate how management practices can impact on soil organic matter. A ‘paired paddock’ demonstration consists of comparing two paddocks, with the same, or at least similar soil type, but with different management histories. During discussions on what soil organic matter is, what its functions are, what it consists of, and what the benefits of soil organic matter are, it is also very important to show farmers how to sample for soil Carbon following SCaRP guidelines. Background information for ‘Paired Paddocks’ Select two paddocks (often on adjoining farms) with similar soil types but with different histories (eg. pasture vs. cropping, or NoTill vs. cultivation, or long term NoTill vs. short term NoTill, or with sheep vs. without sheep etc.) See attached as an example of a paired paddock comparison Paddock History: For each paddock collect 5 years of historical information (eg. 2008 to 2012): (i) Crop type, fallow or pasture (include crop yield) (ii) Grazing pressure (light, moderate or heavy) (iii) Tillage (how often per year) (iv) Crop establishment: NoTill, Traditional cultivated seed bed etc (v) N fertiliser use (vi) Take a photo of the paddock when soil sampling In the paddock: sampling for (i) soil Carbon and (ii) Bulk Density Sampling for Soil C is relatively straight forward. Unfortunately without Bulk Density (BD) it is not possible to calculate the quantity (kg/ha) of Soil C in a paddock. We have to determine both Soil C and BD for a minimum of ten sampling sites per paddock so that the variability within the paddock can be determined - this is important if the paddock is sampled again in the future because without a measure of variability you cannot accurately compare Soil C levels over time. What you need: GPS For Soil C: A soil tube (around 40mm diam) and the means to push/hit it accurately into the ground For Soil BD: the proper set up for measuring BD using undisturbed rings (see note on BD below) Tape measure Duct tape to mark 10 and 30 cm on the soil tube Ruler Spade Soil bags either sealed plastic or strong paper bags clearly marked with the date, owners name, paddock name, sample site number, sampling depth and BD or SC. 1 NOTE on Bulk Density sampling: We prefer using the undisturbed ring technique for bulk density sampling, but appreciate that most groups may not have access to this equipment nor had the training on how to use it. An excellent reference to Bulk Density sampling can be found in ‘Soil Matters, by Neale Dalgliesh and Mike Foale 1998, published by APSRU. You can download the whole book or sections from the web: www.apsim.info/Wiki/public/Upload/ApSoil/SoilMatters You will find the information on sampling for Bulk Density in Section 4 (Determining plant available water capacity). Just briefly, there are two techniques: (i) using undisturbed rings which is the most accurate but also the most labour intensive (and you need the equipment); and (ii) using a normal tube used for soil sampling. NOTE of warning: one reason why this technique tends to be less accurate is that you need to use a large diameter tube, anything less than a 75mm diameter tube is not good enough (see Soil Matters, p 77). Step wise: 1. Determine with the owner of the paddock the main soil types in the paddock. Draw a mud map of the main soil types in the paddock. Make sure you include some easily identifiable features in the paddock (locate north, road, gate, dam, trees etc). 2. Sample the same soil type in each ‘paired paddock’. 3. On the selected soil type sample for Soil Carbon at 10 randomly distributed sampling sites at two depths (0-10 and 10-30cm), locate rough position of each site on your mud map and record each site with a GPS lat/long, numbering them 1 to 10. 4. At each of the 10 sampling sites for Soil Carbon you will also have to collect Bulk Density samples for both the topsoil (0-10cm) and subsoil (10-30cm). You will need to take 10 BD topsoil samples regardless if you are using the ‘tube’ or ‘ring’ technique (there is high variability in BD across the paddock in the topsoil). For subsoil BD sampling we take 5 samples (every second sampling site) when using the rings; and 10 if using a soil tube (because of the decreased accuracy). 5. On the soil tube, accurately measure and mark with duct tape, 10 cm and 30 cm from the base of the tube. Measure and record the diameter of the soil tube with callipers (mm). 6. NOTE: Do NOT Use CRC, WD40 or Canola Oil LUBRICANTS. THESE ALL CONTAIN CARBON! If a lubricant is required use only a Silicone based product. 7. Always sample between the crop rows. If the paddock is in pasture, sample randomly or if you can see old crop rows sample between the rows. 8. When you have selected a sampling site, cut any vegetation to ground level and remove. Carefully scrape off any loose vegetative material from the sampling site. 2 9. SOIL CARBON 0-10. On the cleared area push or hit the tube into the topsoil so that the 10 cm mark is level with the soil surface, remove the tube and collect the 0-10cm soil for the Soil Carbon measurement and put into a labelled bag. You may have to take more than one sample to ensure at least 200g is collected. 10. SOIL CARBON 10-30. Using a spade, carefully dig away the topsoil to 10 cm (measure this accurately!) so that none of the topsoil can fall into the hole for the 10-30 sample. Push (with a soil sampling rig) or hit (with a sledgehammer) the tube into the ground until the 30 cm mark is level with the surface of the 10cm deep platform. Remove the tube and collect the 10-30cm sample into a labelled bag. 11. Move to an undisturbed sample area next to the last hole and cut any vegetation to ground level and remove. Carefully scrape off any loose vegetative material from the sampling site. 12. Bulk Density 0-10. (i) Ring technique: drive the ring assembly into the soil till you have reached the exact depth of the ring (making sure you don’t compress the sample!). Carefully extract the ring assembly to ensure no soil is lost from the bottom of the ring. Clean soil flat with the top and bottom of the ring and empty the ring into a marked sealed plastic or paper bag for drying and weighing. (ii) Tube technique: push or drive the tube into the soil to exactly 10 cm level with the soil surface. Gently remove the tube without losing any soil from the base of the tube. Put all of the soil into a marked sealed plastic or paper bag for drying and weighing. Measure the exact depth of the hole with a measuring tape, to the nearest mm, and record on the bag if it is not exactly 10cm. 13. Bulk Density 10-30. (i) Ring technique: With a spade dig away the soil to about 15cm depth and make a small working platform so that topsoil cannot fall into the subsoil sample. Drive the ring assembly into the soil till you have reached the exact depth of the ring (making sure you don’t compress the sample!). Carefully extract the ring assembly to ensure no soil is lost from the bottom of the ring. Clean soil flat with the top and bottom of the ring and empty the ring into a marked sealed plastic or paper bag for drying and weighing. (ii) Tube technique: Using a spade dig away the soil to 10 cm (measure with ruler) and make a small working platform so that none of the topsoil can fall into the 10-30 sample. Drive the tube into ground to the 20cm mark where it is exactly level with the soil surface of the platform at 10cm depth. Gently remove the tube making sure you don’t lose any soil from the base of tube. Put the 10-30 cm sample into a marked and sealed plastic or paper bag for drying and weighing. Measure the exact depth of the hole you have made with a measuring tape, to the nearest mm, and record on the bag. If you loose soil from the tube or bag you will need to repeat the sample. 14. Repeat for each of the 10 sites. 3 In the Laboratory Soil C analysis 1. Dry the samples for Soil Carbon analysis at 40oC in a force draught oven for 48 hours. After drying, store samples in labelled bags until they are dispatched to the laboratory. Bulk Density samples 1. Prepare a lab sheet and record: > paddock name > sample number and depth > aluminium tray number (if the samples are in strong paper bags they can stay in the bag) > tray or paper bag weight (in grams, to one decimal place) > wet weight (in grams, to one decimal place) of the soil (soil + tray) 2. Place in a force draught oven at 105oC for 72 hours. Take out of the oven and record dry weight (soil + tray). (A wet heavy clay may take longer than 72 hours to dry). 3. If there is gravel, buckshot or rocks in the sample you will need to sieve the sample to pass through a 2mm sieve. Separately weigh the gravel or rocks remaining on top of the sieve, record weight. 4. Determine the diameter of the ring or tube you used in the paddock. With callipers measure the inside diameter of the tube. Use the accompanying spreadsheet to calculate the bulk density (Bulk density calculator.xlsx). Note the calculation is (as a guide BD should be between 0.9 and 1.8 g/cm3): BD = (Weight of Dry Soil – Tray or bag weight – Gravel weight) (Volume of soil core – Gravel weight / 2.65) Calculation of Soil C content in the paddock 5. When you get the Soil C results from the lab (and its fractions) you can calculate the amount of soil Carbon in the paddock using: http://soilquality.org.au/calculators/gravel_bulk_density It is an easy to use web site and the calculations are straight forward. Harm van Rees 31/08/2012 4 A few photos with handy hints Clear stubble and fine material away to make a clean working surface soil Hammer tube into the ground (for the deep samples we used a sledge hammer) Have a ruler level to the ground so you can easily see the depth you need to sample to BD ring assembly BD sampling topsoil BD sampling subsoil 5 Check depth with a tape measure (up against the ruler), record if it is not exactly 30cm Check to make sure there is a clear break of soil at the bottom of the tube (twist the tube before pulling it out) Working example only Farmers leading and learning about the soil carbon frontier Paired paddock comparison for soil carbon Normanville Group August 2012 Mallee alkaline clay loam, gentle slope towards the southwest Contrast in Land-use: Main issues: Year: Paddock history: Yield (t/ha): N inputs (kg N/ha): Grazing: Total Soil Carbon % Total Nitrogen % C:N ratio Bulk density g/cm3 Particulate Carbon kg/ha Humus kg/ha Charcoal kg/ha Bremner Hunt p17 35.52.10oS, 143.41.19oE 35.52.09oS, 143.41.26oE No Till stubble retained Traditional cultivated crop establishment Does long term NoTill stubble retained systems have higher soil Carbon levels compared to traditional crop establishment practices? Are there differences in humus content (stable Soil C fraction) between the two management systems? How can you raise soil Carbon levels in cropping soils? 2008 2009 2010 2011 2012 2008 2009 2010 2011 2012 Canola Wheat Wheat Barley Canola 0.1 1.3 3.8 4.2 xx 2.5 9.1 2.4 34.4 43.1 Light grazing on agistment over summer Topsoil (0-10cm): 1.29 Subsoil (10-30cm): 1.42 Nitrous Oxide emissions kg/ha CO2e (2008 to 2012) from N fertiliser 6 Topsoil (0-10cm): Subsoil (10-30cm):