Table S5: Eigen values, loadings, and scoring coefficients from a Discriminant Function Analysis (DFA) on 9 acoustic parameters measured from calls recorded from male Pseudacris crucifer in allopatry and sympatry during 2003-2011. For 6 of these we used residuals from separate regressions on temperature (Table S4). Also included is a DFA of 9 acoustic parameters controlling both for body size (SVL) and temperature. A ‘t’ denote acoustic parameters that were corrected for temperature for the first DFA, and an ‘m” indicates which were corrected for body size as well for the second DFA. Canon. Axes Eigen Value Cum. Percent Scoring Coefficients Max Freqt,m Min Freqt,m Delta Freq Call Rise Time Call Fall Time Carrier Lengtht,m Call Call t,m Interval Dur t,m Call Rate t,m 1 0.35 62.0% 0.43 -0.17 0.11 0.47 0.58 0.99 0.53 -0.96 0.36 2 0.12 83.7% -1.66 1.36 -0.18 -0.34 0.37 0.14 -0.09 0.64 -0.14 3 0.06 94.7% 0.66 -0.97 -0.88 0.05 -0.05 -0.09 1.74 -0.55 1.19 Correcting for body size & temperature 1 0.35 62.9% -0.002 -0.002 -0.0002 -4.75 4.71 14.40 0.11 -5.46 0.14 2 0.17 92.8% 0.004 0.004 -0.0001 -29.86 29.87 4.20 0.75 5.25 -0.26 3 0.04 99.8% 0.005 0.005 0.0010 -26.87 26.88 8.68 -1.01 17.32 -0.45