Exome-Sequencing, with Prior Linkage Analysis, Identifies a Candidate Gene Causing Maturity-Onset Diabetes of the Young (MODY) in Thais Family Ninareeman Binnima1,* , Watip Tangjittipokin1, Kanchana Chanprasert1, Jatuporn Sujjitjoon1, Prapaporn Jungtrakoon1, Pa-thai Yenchitsomanus2, Nattachet Plengvidhya3,# 1 Department of Immunology and Immunology Graduate Program, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand 2 Division of Molecular Medicine, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand 3 Division of Endocrinology and Metabolism, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand *en_nina@hotmail.com, #sinpv.natpl@gmail.com Abstract Maturity-onset diabetes of the young (MODY) is a monogenic form of diabetes. It is characterized by an early onset (usually before age 25 years), non-insulin dependence and autosomal dominant inheritance. Genetic factors play crucial roles in development of MODY. Recently, MODY is caused by mutation in at least thirteen different genes including HNF-4α, GCK, HNF-1α, IPF-1, HNF-1β, NeuroD1, KLF11, CEL, PAX4, INS, BLK, ABCC8 and KCNJ11. However, MODY with unknown genetic etiology, or MODY-X, is still common in many ethnic groups. Because 80% of Thai MODY are MODY-X, this study aimed to identify novel MODY genes by linkage analysis and exome-sequencing. Linkage analysis was done in 27 members from a Thai MODY-X family. LOD score ≥ 2.5 was identified in chromosome 9, 11 and 22. Exome-sequencing was executed in two affected and two unaffected family members. Two novel variants, PTCH1 p.M1071L and GGT5 p.A48T, located in these high LOD regions were selected. Four in silico programs including PolyPhen V2.0.23, SIFT, Mutation Taster and VarioWatch were used to predict the possible deleterious effect of these certain variants on protein functions. Only PTCH1 p.M1071L has deleterious effects. This variant was validated by Sanger sequencing and genotyped in family members. PTCH1 p.M1071L was partially segregated with diabetes in this family. This variant was not detected in 200 non-diabetic controls and other 66 MODY-X probands. Interestingly, PTCH1 is expressed in pancreatic islets and involved in islets development and function. Therefore, PTCH1 may be a pathogenic gene causing MODY in this family. Thus, linkage analysis combined with exome-sequencing is a new strategy to identify novel MODY genes Acknowledgements: This work was supported by Siriraj Graduate Thesis Scholarship, Siriraj Grant for Research and Development, Mahidol University Grant and Thailand Research Fund. Keywords: maturity-onset diabetes of the young, linkage analysis, exome-sequencing, MODY, diabetes