GRADE 9 Biotechnology Cycles 8-20 (First 7 cycles – explore)
Cycle Overview
8
Lab Safety, Media preparation, metric system and tools,
Lab notebook and documentation, History of the biotechnology industry
Sterile techniques, pipetting, equipment overview, comparison between rennin and chymosin
in cheese production
9
Atoms, ions, carbon-based molecules, macromolecules – nucleic acids, carbohydrates, lipids,
proteins
History of the biotechnology industry (cont.)
Gram stains, estimation of microscopic field size, solution preparation, density studies,
macromolecule assays
10
Macromolecules – nucleic acids, carbohydrates, lipids, proteins (cont.)
Yeast as a model organism, growth conditions of model organisms, growth curves
Agarose gel electrophoresis, DNA structure, replication
11
Enzyme activity, DNA structure, replication, PCR
Identification of lagomorph species using restriction fragment length polymorphism of DNA
12
Enzyme activity, RNA transcription and translation, proteins
BCA protein assay, gel analysis of DNA restriction digests using lambda DNA
13
RNA transcription and translation (cont.)
Proteins (cont).
Breakdown of hydrogen peroxide by catalase
14
Proteins (cont.) History of drug development
Testing plants and other substances for antibacterial activity
15
16
Lagomorph species identification using DNA isolation and analysis:
DNA isolation, use of mitochondrial DNA for species identification, enzyme site polymorphisms,
gel analysis of DNA fragment polymorphisms, PCR, optimal enzyme conditions.
Polyacrylamide Gel Electrophoresis (PAGE) and bioethics
Identification of unknown bacterial species, differential indicators and growth conditions, gram
stains, streaking for isolation
17
Polyacrylamide gel electrophoresis (cont.)
Comparison of chicken and beef skeletal muscle, preparation of cell lysate, gel electrophoresis,
evolutionary studies based on conservation of structural proteins, levels of protein structures,
SDS ionic interactions
18
Assay development
Amylase assay using recombinant bacterial amylase, dilution series, spectrophotometer,
enzyme assays, time points, graphing, starch molecule biochemistry, types of enzyme activities,
substrate depletion, product accumulation, Benedicts test, quantitative versus qualitative data
19
Bioethics of DNA testing (May 2013 supreme court ruling on DNA testing)
Crime Scene Investigation (CSI)!!!! Chromatography, Polymerase Chain Reaction (PCR) and gel
analysis, Combined DNA Information System, polymorphic genetic loci, allelic variation,
resolution of light microscope, pollen morphology,
20
Bioethics of DNA testing (May 2013 supreme court ruling on DNA testing)
Crime Scene Investigation (CSI)!!!! Chromatography, Polymerase Chain Reaction (PCR) and gel
analysis, Combined DNA Information System, polymorphic genetic loci, allelic variation,
resolution of light microscope, pollen morphology,