Estimation of haemoglobin concentration Principle :-To estimate the concentration of haemoglobin in the provided sample of blood. Sahli Method:Preparation:a) Fill haemometer tube to level (0.02g) with standard hydrochloric acid diluted 1:10. b) Place ready water for dilution,clock with second for checking reaction time. c) Sterilize finger tip and prick it with lancet and suck blood into capillary pipette upto 20cu.mm. d) Blow blood into hydrochloric acid in the tube. e) Put the tube in the stand. f) Dilute with pure water until color the same. g) Read the result after 3 minutes. Discussion:Haemoglobin and abbreviated Hb is the iron – containing oxygen transport metalloprotein in the red blood cells. Haemoglobin concentration measurement is among the most commonly performed blood tests, usually as part of a complete blood count. Result are reported in g/L,g/dl . Men 13.5 to 16.5 g/dl Women 12 to 15 g/dl Children 11 to 16 g/dl Pregnant women 11 to 12 g/dl If the concentration is below normal, this is called anemia. Anemia are classified by the size of red blood cells . The anemia is called microcytic if red cells are small. Macrocytic if they are large,and normocytic otherwise.