Program Director/Principal Investigator (Wolf-Yadlin, Alejandro):
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors in the order listed on
Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
Alejandro Wolf-Yadlin
POSITION TITLE
Assistant Professor
Department of Genome Sciences
University of Washington
eRA COMMONS USER NAME (credential, e.g., agency
login)
ayadlin
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include
postdoctoral training and residency training if applicable.)
DEGREE
INSTITUTION AND LOCATION
MM/YY
FIELD OF STUDY
(if applicable)
Universidad de Chile. Santiago, Chile
B.S
07/1999
Biotechnology Engineering
Universidad de Chile. Santiago, Chile
Engineering
04/2001
Biotechnology Engineering
Universidad de Chile. Santiago, Chile
M.S
04/2001
Chemical Engineering
Massachusetts Institute of Technology. Cambridge,
PHD
02/2007
Bioengineering
MA
Harvard University. Cambridge, MA
Postdoc
06/2010
Systems Biology
A. Personal Statement
The overarching goal of our research program focuses on the study of cellular signaling networks, their relationship to
gene expression and cellular phenotype. During my first year at UW I have been developing the framework to apply this
approach to projects in basic cell biology as well as cancer biology and neurodegenerative and infectious diseases. More
specifically, our laboratory focuses on the development and application of new proteomic based technologies to
understand cellular signaling networks in the context of cellular homeostasis and disease. We use mass spectrometry to
detect and quantify changes in protein expression, post-translational modifications and protein-protein and protein-DNA
interactions over a wide range of biological conditions. In parallel, we have developed and are currently using
high-throughput techniques to monitor gene expression and cellular phenotype under the same biological conditions we
use for proteomics analysis. Finally, we integrate these datasets using and/or developing computational tools to determine
the structure and dynamic behavior of signaling and gene-expression networks to reveal causal relationships between our
distinct classes of measurements.
Although, we are interested in various types of post-translational modifications, currently our main focus is protein
phosphorylation. Phosphorylation is one of the most common regulatory mechanisms in the cell. It plays a role in
determining various cellular outcomes including proliferation, migration, gene expression, apoptosis, cellular size/shape
and differentiation. The question we aim to answer is how dynamic changes in phosphorylation regulate these outcomes
and how the information encoded by these modifications is integrated into a single coherent biological response. We are
doing this by attempting to find causal relationships between protein phosphorylation, protein-protein interactions, gene
expression and cell phenotype. We hope that our efforts will provide a better understanding of the cell and highlight
network features that correlate or cause changes in gene expression and/or cell behavior in disease states.
B. Positions and Honors
Positions and Employment
2007-2010
Postdoctoral Fellow, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA
2011Assistant Professor, Department of Genome Science, University of Washington, Seattle, WA
Other Experience and Professional Memberships
2011Member of STRIDE Center for Systems & Translational Research on Infectious Disease, University of
Washington
2011Member of Proteomic Resource, University of Washington
2011Member of American Society of Mass Spectrometry
Honors
2000
2001-2002
European Community alpha scholarship for graduate students
Du Pont Fellowship for first year graduate students at MIT
PHS 398/2590 (Rev. 06/09)
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Biographical Sketch Format Page
Program Director/Principal Investigator (Wolf-Yadlin, Alejandro):
2005
2006
2009
2010
2012
2012
2013
Best Poster award GTCbio 2nd International Conference on Tumor Progression & Therapeutic
Resistance
Daniel K. Ludwig Fellowship recipient for cancer research
Awarded Best Poster at System Biology for Human Disease Conference
Invited lecturer: “Lecture Series on Proteomics” at Cold Spring Harbor
Laboratory
Invited reviewer for SkTech Research Center Development Proposals. The Skolkovo Institute of Science
and Technology (SkTech) is a new graduate university outside of Moscow, Russia
Invited lecturer: “Lecture Series on Proteomics” at Cold Spring Harbor
Laboratory
Invited reviewer for SkTech Research Center Development Proposals. The Skolkovo Institute of Science
and Technology (SkTech) is a new graduate university outside of Moscow, Russia
C. Selected Peer-reviewed Publications (Selected from 10 peer-reviewed publications)
Most relevant to the current application
1. * Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, White FM. “ Time-resolved mass
spectrometry of tyrosine phosphorylation sites in the EGF receptor signaling network reveals dynamic modules”. Mol
Cell Proteomics. 2005; 4(9):1240-50. Epub 2005 Jun 11.
2. Wolf-Yadlin A, Kumar N, Zhang Y, Hautaniemi S, Zaman M, Kim HD, Grantcharova V, Lauffenburger DA, White FM.
“Effects of HER2 overexpression on cell signaling networks governing proliferation and migration”. Mol Syst Biol.
2006; 2:54. Epub 2006 Oct 3.
3. Kumar N, Wolf-Yadlin A, White FM, Lauffenburger DA. “Modeling HER2 Effects on Cell Behavior from Mass
Spectrometry Phosphotyrosine Data”. PLoS Comput Biol. 2007; 3(1):e4. Epub 2006 Nov 21.
4. * Zhang Y, Wolf-Yadlin A, White FM. “Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular
Signaling Networks”. Methods Mol Biol. 2007; 359(14):203-212.
5. Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM. “Multiple Reaction Monitoring for Robust Quantitative
Proteomic Analysis of Cellular Signaling Networks”. Proc Natl Acad Sci U S A. 2007; 104(14):5860-5. Epub 2007 Mar
26.
6. Navare A, Sova P, Purdy DE, Weiss JM, Wolf-Yadlin A, Korth MJ, Chang ST, Proll SC, Krasnoselsky AL, Palermo
RE, Katze MG. “Quantitative Proteomic Analysis of HIV-1 Infected CD4+ T Cells Reveals an Early Host Response in
Important Biological Pathways: Protein Synthesis, Cell Proliferation, and T-cell Activation”. Virology. 2012; 429(1):3746.
7. Dai X, James RG, Habib T, Singh S, Jackson S, Khim S, Moon RT, Liggitt D, Wolf-Yadlin A, Buckner J, and Rawlings
D. “The PTPN22 risk variant promotes systemic autoimmunity by altering lymphocyte selection and activation”.
Accepted by Journal of Clinical Immunology. Reference 66963-RG-RV-3.
8. Moore KE, Carlson SM, Cheung P, Chua KF, Wolf-Yadlin A, Gozani O. “Engineering a Natural Affinity Reagent for
Detection and Proteomic Analysis of Lysine Methylation”. Accepted by Molecular Cell. Reference D-12-01894R2.
9. James RG, Bosch KA, Yang PT, Robin NC, Toroni RA, Kulikauskas RM, Biechele TL, Berndt JD, von Haller PD, Eng
J, Wolf-Yadlin A, Chien AJ, and Moon RT. "Protein Kinase PKN1 represses Wnt/ß-catenin signaling in human
melanoma cells”. Submitted.
10. Wagner JP*, Wolf-Yadlin A*, Sevecka M, Grenier JK, Root DE, Lauffenburger DA, and MacBeath G. “Receptor
Tyrosine Kinases Fall Into Distinct Classes Based on Their Inferred Signaling Networks”. Submitted.
*
In all papers marked with an * Wolf-Yadlin A is co-first author.
Additional recent publications of importance to the field (in chronological order)
1. Gordus A, Krall JA, Beyer EM, Kaushansky A, Wolf-Yadlin A, Sevecka M, Chang BH, Rush J, MacBeath G. “Linear
combinations of docking affinities explain quantitative differences in RTK signaling”. Mol Syst Biol. 2009; 5:235. Epub
2009 Jan 20.
2. Wolf-Yadlin A, Sevecka M, MacBeath, G. “Dissecting Protein Function and Signaling Using Protein Microarrays”.
Curr. Opin. Chem. Biol. 2009; 13(4):398-405. Epub 2009 Aug 5.
3. Locasale JW, Wolf-Yadlin A,“Maximum entropy reconstructions of dynamic signaling networks from quantitative
proteomics data”. PLoS One. 2009 Aug 26; 4(8):e6522.
4. * Sevecka M, Wolf-Yadlin A, MacBeath, G “Lysate Microarrays Enable High-throughput, Quantitative Investigations of
Cellular Signaling”. Mol Cell Proteomics. 2011 Apr; 10(4):M110.005363. Epub 2011 Feb 4.
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Program Director/Principal Investigator (Wolf-Yadlin, Alejandro):
D. Research Support
Startup Funds Genome Sciences University of Washington
NIH 1DP2OD017358-01, pending
Mallinckrodt Foundation Grant 201302 deadline, pending
Role: PI
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