Alysa and Dani
Table One:
DNA Size
Markers
Fragment 1
Fragment 2
Fragment 3
Fragment 4
Fragment 5
Fragment 6
Fragment 7
Fragment 8
Fragment
Length in Base
Pairs
Distance
Migrated (mm)
A
1353
1078
872
603
310
281
234
194
28
34
38
49
70
73
78
84
Distance to
Reference
Point (mm)
B
109
109
109
109
109
109
109
109
Rf
A÷B
.26
.31
.35
.45
.64
.67
.72
.77
1. Obtain a Student Response Sheet from your teacher.
2. Draw a standard curve plotting Rf versus fragment length (in base pairs) on your
Student Response Sheet.
3. Calculate the Rf value for each DNA fragment for each family member and fill-in
the table below. Round values to the nearest one hundredth.
DNA Sample:
Diana
Jennifer
Laura
Judy
Fragment:
Fragment 1
Fragment 2
Fragment 1
Fragment 2
Fragment 1
Fragment 2
Fragment 1
Fragment 2
Distance
Migrated (mm)
A
47
56
51
63
47
63
37
63
Distance to
Reference
Point (mm)
B
109
109
109
109
109
109
109
109
Rf
A÷B
.43
.51
.47
.58
.43
.58
.34
.58
4. Use your standard curve to determine the base pair size of the fragments of all
family members’ DNA samples and add this information to Table Two.
Table Two:
DNA Sample:
Diana
Jennifer
Laura
Fragment:
Fragment 1
Fragment 2
Fragment 1
Fragment 2
Fragment 1
Fragment Length (in
base pairs)
700
500
600
400
600
Allele Present:
6
4
5
3
5
Judy
Fragment 2
Fragment 1
Fragment 2
400
900
400
3
8
3
5. Use the data table below to determine which alleles were present in each family
member’s DNA samples. Fill in the Allele Present column in Table Two.
Fragment Length in Base Pairs:
Allele:
200
300
400
500
600
700
800
900
1000
Allele 1
Allele 2
Allele 3
Allele 4
Allele 5
Allele 6
Allele 7
Allele 8
Allele 9