90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals 100 East 77th Street New York, N.Y. 10075 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Name, Position Author/Approvin g Authority: Oana Vele, MD Director of Clinical Pathology Date Issued: 6/7/2010 Annual Review/ Revisions Date Signature Signature on file 2/05, 5/09, 5/10, 2/11, 9/12, 3/02 06/2014; 01/2015; 02/16/15; 10/2015 Oana Vele, MD - signature on file Signature Date 6/7/2010 Date Signature 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 OVERVIEW: CHEMISTRY FLUIDS CHEMISTRY URINE CHEMISTRY HEMATOLOGY COAGULATION SEROLOGY MISCELLANEOUS APPENDIX 2-8 8-10 10-13 13-14 14-16 16-18 19 20-23 METHOD KEY: C CER CIA CMIA D DSI EIA IR LOCI LA N PA PETINIA R RICA Colorimetric Coupled enzyme reaction Chemiluminescent Immunometric Assay Chemiluminescent Microparticle Immunoassay Dipstick Direct Solid-phase Immunoassay Enzyme Immunoassay Immuno Rate Luminescent Oxygen Channeling Assay Latex agglutination Nephelometric Particle agglutination Particle Enhanced Turbidimetric Inhibition Immunoassay Rate bichromatic Rapid Immunochromatographic Assay Page 1 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 COLLECTION TUBE REQUIREMENTS AND NORMAL INTERVALS CHEMISTRY METHOD SEE INDIVIDUAL TEST SEE INDIVIDUAL TEST PANEL/TEST NAME BASIC METABOLIC PANEL COMPREHENSIVE METABOLIC PANEL TEST(S) SODIUM, POTASSIUM, CHLORIDE, CARBON DIOXIDE, CALCULATED ANION GAP GLUCOSE, BUN, CREATININE, CALCULATED EGFR, CALCIUM SODIUM, POTASSIUM CHLORIDE, CARBON DIOXIDE, CALCULATED ANION GAP, GLUCOSE, BUN CREATININE, CALCULATED EGFR, CALCIUM TOTAL PROTEIN, ALBUMIN TOTAL BILIRUBIN, ALKALINE TUBE NORMAL INTERVAL MINT TOP SEE INDIVIDUAL TEST ANION GAP: 9-16 MMOL/L MINT TOP SEE INDIVIDUAL TEST PHOSPHATASE ALT/SGPT, AST/SGOT SEE INDIVIDUAL TEST CARDIAC PANEL CREATINE KINASE, MMB FRACTION, TROPONIN I MINT TOP SEE INDIVIDUAL TEST TOTAL CK WITH MMB CREATINE KINASE, MASS OF MB ISOENZYME (MMB) FRACTION CALCULATED MMB/CK % WHEN MMB >=5 NG/ML MINT TOP MMB 0.5-3.6 NG/ML MMB/CK % =<4% ELECTROLYTE PANEL SODIUM, POTASSIUM, CHLORIDE, CARBON DIOXIDE MINT TOP SEE INDIVIDUAL TEST HEPATIC FUNCTION PANEL TOTAL PROTEIN, ALBUMIN TOTAL BILIRUBIN, DIRECT BILIRUBIN, CALCULATED INDIRECT BILIRUBIN, ALKALINE PHOSPHATASE ALT/SGPT, AST/SGOT MINT TOP SEE INDIVIDUAL TEST R AND LOCI SEE INDIVIDUAL TEST SEE INDIVIDUAL TEST Page 2 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 SEE INDIVIDUAL TEST MINT TOP SEE INDIVIDUAL TEST FOR LDL-C <100 MG/DL OPTIMAL 100-129 MG/DL LOW RISK 130-159 MG/DL BORDERLINE HIGH 160-189 MG/DL HIGH 190 MG/DL OR > VERY HIGH GOLD TOP SEE INDIVIDUAL TEST TRANSFERRIN SATURATION 20-38 % FEMALES 26-39 % MALES LIPID PANEL CHOLESTEROL HDL-C, LDL-C (CALCULATED VALUE), TRIGLYCERIDES SEE INDIVIDUAL TEST IRON PANEL IRON TIBC TRANSFERRIN SATURATION (CALCULATED) FERRITIN C ACETAMINOPHEN ACETAMINOPHEN GOLD TOP THERAPEUTIC INTERVAL: 10.0-30.0 MCG/ML C ALBUMIN ALBUMIN MINT TOP 3.4-5.0 GM/DL R ALCOHOL ALCOHOL GOLD TOP < 3 MG/DL R ALKALINE PHOSPHATASE ALKALINE PHOSPHATASE MINT TOP 0 days 40-350 U/L 10 years 40-280 U/L 15 years 40-150 U/L 20 years 45-117 U/L MINT TOP 12-42 U/L 10-32 MCMOL/L R ALT/SGPT SERUM ALANINE AMINOTRANSFERASE GLUTAMIC PYRUVATE TRANSAMINASE R AMMONIA AMMONIA MINT TOP ON ICE R AMYLASE AMYLASE MINT TOP R AST/SGOT C BETA HYDROXY BUTYRATE ASPARATE AMINOTRANSFERASE GLUTAMATE OXALACETATE TRANSAMINASE BETA HYDROXYBUTYRATE, SERUM MINT TOP 25-115 U/L 15-37 U/L 0.0 - 0.4 MMOL/L GOLD TOP Page 3 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 LOCI C C CALCULA TED BETA HUMAN CHORIONIC GONADOTROPIN QUANTITATIVE DIRECT BILIRUBIN TOTAL BILIRUBIN BOTH DIRECT AND TOTAL BILIRUBIN ARE TESTED NON-PREGNANT FEMALES, AGES 18-62: 1-3 MIU/ML BHCG, QUANTITATIVE DIRECT (CONJUGATED) BILIRUBIN MINT TOP MINT TOP MALES, AGES 19-67: =< 1 MIU/ML 0-0.2 MG/DL MINT TOP 0 days 2.0-6.0 MG/DL 1 day 6.0-10.0 MG/DL 3 days 4.0-8.0 MG/DL 6 days 0.2-1.2 MG/DL 29 days 0.2-1.2 MG/DL 18 y.o. 0.2-1.0 MG/DL INDIRECT BILIRUBIN MINT TOP 0 days 2.0-5.8 MG/DL 1 day 6.0-9.8 MG/DL 3 days 4.0-7.8 MG/DL 6 days 0.2-1.2 MG/DL 29 days 0.2-1.0 MG/DL TOTAL BILIRUBIN R BLOOD UREA NITROGEN BLOOD UREA NITROGEN MINT TOP 7-23 MG/DL C CALCIUM CALCIUM MINT TOP 8.5-10.5 MG/DL N CARBAMAZEPINE CARBAMAZEPINE MINT TOP THERAPEUTIC INTERVAL: 4.0-12.0 MCG/ML C CARBON DIOXIDE P CHLORIDE C CMIA CMIA CHOLESTEROL CARBON DIOXIDE, TOTAL CHLORIDE MINT TOP ADULTS: 22-31 MMOL/L NEONATES: 13-22 MMOL/L MINT TOP 96-108 MMOL/L MINT TOP < 200 MG/DL DESIRABLE 200-239 MG/DL BORDERLINE HIGH >239 MG/DL HIGH CHOLESTEROL CORTISOL A.M. A.M. CORTISOL LEVEL 8-10 A.M. GOLD TOP 3.9- 37.5 MCG/DL CORTISOL P.M. P.M. CORTISOL LEVEL 4-6 P.M. GOLD TOP 2.3-13.8 MCG/DL Page 4 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 N C-REACTIVE PROTEIN R CREATINE KINASE CE CREATININE CKD-EPI EQUATION CALCULATED WHEN CREATININE IS TESTED on PATIENTS >18 Y.O. R LOCI LOCI DIGOXIN FERRITIN FREE TRIIODOTHYRONINE C-REACTIVE PROTEIN CREATINE KINASE MINT TOP < 0.30 MG/DL MINT TOP FEMALE: 26-192 U/L MALE: 39-308 U/L MINT TOP 0 days 0.20-0.70 MG/DL 10 years 0.50-1.30 MG/DL MINT TOP >=60 ML/MIN MINT TOP THERAPEUTIC INTERVAL: 0.8-2.0 NG/ML GOLD TOP FEMALE:8-252 NG/ML MALE: 26-388 NG/ML CREATININE, IDMS ESTIMATED GLOMERULAR FILTRATION RATE DIGOXIN FERRITIN FREE TRIIODOTHYRONINE MINT TOP 1.71-3.71 PG/ML MINT TOP 0.70-1.48 NG/DL LOCI FREE THYROXINE FREE THYROXINE R GAMMA GLUTAMYL TRANSFERASE GAMMA GLUTAMYL TRANSFERASE (GGT) N GENTAMICIN TROUGH GENTAMICIN TROUGH GOLD TOP THERAPEUTIC INTERVAL: 0-2.0 MCG/ML N GENTAMICIN PEAK GENTAMICIN PEAK GOLD TOP THERAPEUTIC INTERVAL: 4.0-8.0 MCG/ML N GENTAMICIN RANDOM GENTAMICIN RANDOM C GLUCOSE, NON FASTING, PART OF BMP AND CMP GLUCOSE MINT TOP C GLUCOSE, FASTING GLUCOSE GRAY TOP MINT TOP GOLD TOP FEMALE: 5-55 U/L MALE: 15-85 U/L NO REFERENCE RANGE 70-99 MG/DL 70-99 MG/DL Page 5 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 GRAY TOP C GLUCOSE, RANDOM GLUCOSE C GLUCOSE CHALLENGE TEST, GESTATIONAL SCREEN (50 G GLUCOSE LOAD) GLUCOSE C C C ORAL GLUCOSE TOLERANCE TEST 2 HRS (75 G GLUCOSE LOAD) ORAL GLUCOSE TOLERANCE TEST 3 HRS (100 G GLUCOSE LOAD) GLUCOSE, 2 HRS POST GLUCOLA (75 G GLUCOSE LOAD) GRAY TOP GRAY TOP GLUCOSE GRAY TOP <140 MG/DL GRAY TOP <140 MG/DL N HAPTOGLOBIN HAPTOGLOBIN PETINIA HEMOGLOBIN A1C PERCENTAGE OF GLYCATED HEMOGLOBIN C HDL-C HDL-C CMIA INTRAOPERATIVE PARATHORMONE LACTOSE TOLERANCE TEST (LACTOSE DOSE: 50 G) SEE REPORT GLUCOSE GLUCOSE C SEE REPORT GRAY TOP GLUCOSE, 2 HRS, POSTPRANDIAL LACTIC ACID <140 MG/DL GLUCOSE C C 70-139 MG/DL MINT TOP LAVENDER TOP MINT TOP 31-207 MG/DL 4.8-5.6% <40 LOW (FLAGGED) >=60 HIGH INTRAOPERATIVE PARATHORMONE LAVENDER TOP 8.5-72.5 PG/ML LACTIC ACID, WHOLE BLOOD DARK GREEN TOP ON ICE 0.5-2.0 MMOL/L GLUCOSE GRAY TOP SEE REPORT Page 6 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 R R LACTATE DEHYDROGENASE LIPASE LACTATE DEHYDROGENASE MINT TOP FEMALE: 84-246 U/L MALE: 87-241 U/L LIPASE MINT TOP 73-393 U/L GOLD TOP THERAPEUTIC INTERVAL: 0.6-1.2 MMOL/L MINT TOP 1.6-2.4 MG/DL MINT TOP FEMALE: 13-71 NG/ML MALE: 16-116 NG/ML C LITHIUM LITHIUM C MAGNESIUM MAGNESIUM LOCI MYOGLOBIN MYOGLOBIN LOCI NT-PRO BNP NT-PRO BNP MINT TOP <300 PG/ML FP OSMOLALITY, SERUM OSMOLALITY GOLD TOP 280-301 MOSM/KG PETINIA PHENOBARBITAL PHENOBARBITAL GOLD TOP THERAPEUTIC INTERVAL: 15-40 MCG/ML C P PHOSPHORUS POTASSIUM, PLASMA N PREALBUMIN CMIA PROSTATE SPECIFIC ANTIGEN PETINIA PHENYTOIN (DILANTIN) C SALICYLATE MINT TOP 0-6 weeks: 4.2 - 9.0 MG/DL 6 weeks-18 months: 3.8 - 6.7 MG/DL 18 months-3 yrs = 2.9 - 5.9 MG/DL 3-15 yrs = 3.6 - 5.6 MG/DL >15yrs = 2.5 - 4.5 MG/DL MINT TOP 3.5-5.1 MMOL/L PHOSPHORUS POTASSIUM PREALBUMIN PROSTATE SPECIFIC ANTIGEN MINT TOP GOLD TOP 0.0-4.0 NG/ML GOLD TOP THERAPEUTIC INTERVAL 10-20 MCG/ML GOLD TOP THERAPEUTIC INTERVAL: 2.8-20.0 MG/DL PHENYTOIN (DILANTIN) SALICYLATE 20-40 MG/DL Page 7 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 P C SODIUM TOTAL PROTEIN SODIUM TOTAL PROTEIN MINT TOP MINT TOP 135-145 MMOL/L 6.4- 8.2 G/DL 3.17-11.72 MCG/DL IR THYROXINE THYROXINE PETINIA THEOPHYLLINE THEOPHYLLINE N TRANSFERRIN TRANSFERRIN C TRIGLYCERIDES TRIGLYCERIDES MINT TOP <150 MG/DL LOCI TROPONIN I TROPONIN I MINT TOP 0.015- 0.045 NG/ML THYROID STIMULATING HORMONE THYROID STIMULATING HORMONE (TSH) MINT TOP LOCI C URIC ACID URIC ACID PETINIA VALPROIC ACID PETINIA VANCOMYCIN TROUGH PETINIA VANCOMYCIN RANDOM VALPROIC ACID MINT TOP GOLD TOP MINT TOP THERAPEUTIC INTERVAL: 10-20 MCG/ML 200-360 MG/DL 0.35-4.94 MCIU/ML MINT TOP FEMALE: 2.6-6.0 MG/DL MALE: 3.5-7.2 MG/DL GOLD TOP 50-100 MCG/ML VANCOMYCIN TROUGH MINT TOP 10-20 MCG/ML VANCOMYCIN RANDOM MINT TOP NO REFERENCE RANGE Page 8 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 FLUIDS CHEMISTRY METHOD PANEL/TEST NAME SAME AS FOR PLASMA BASIC METABOLIC PANEL SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA COMPREHENSIVE METABOLIC PANEL TEST(S) SODIUM, POTASSIUM, CHLORIDE, CARBON DIOXIDE, GLUCOSE, BUN, CREATININE CALCIUM SODIUM,POTASSIUM CHLORIDE, CARBON DIOXIDE , GLUCOSE, BUN, CREATININE , CALCIUM TOTAL PROTEIN , ALBUMIN TOTAL BILIRUBIN , ALKALINE PHOSPHATASE ALT/SGPT, AST/SGOT CONTAINER NORMAL INTERVAL STERILE CONTAINER NO REFERENCE RANGE STERILE CONTAINER NO REFERENCE RANGE ALBUMIN, FLUID ALBUMIN STERILE CONTAINER NO REFERENCE RANGE AMYLASE, FLUID AMYLASE STERILE CONTAINER NO REFERENCE RANGE BILIRUBIN, FLUID BILIRUBIN STERILE CONTAINER NO REFERENCE RANGE CALCIUM, FLUID CALCIUM STERILE CONTAINER NO REFERENCE RANGE CHOLESTEROL, FLUID CHOLESTEROL STERILE CONTAINER CREATININE, FLUID CREATININE STERILE CONTAINER GLUCOSE, FLUID GLUCOSE STERILE CONTAINER NO REFERENCE RANGE NO REFERENCE RANGE NO REFERENCE RANGE Page 9 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA C LACTATE DEHYDROGENASEF LUID LACTATE DEHYDROGENASE STERILE CONTAINER NO REFERENCE RANGE LIPASE, FLUID LIPASE STERILE CONTAINER NO REFERENCE RANGE POTASSIUM, FLUID POTASSIUM STERILE CONTAINER NO REFERENCE RANGE SODIUM, FLUID SODIUM STERILE CONTAINER NO REFERENCE RANGE STERILE CONTAINER NO REFERENCE RANGE STERILE CONTAINER NO REFERENCE RANGE STERILE CONTAINER 12-60 MG/DL TOTAL PROTEIN, FLUID TOTAL PROTEIN TRIGLYCERIDES, FLUID TRIGLYCERIDES CEREBROSPINAL FLUID PROTEIN C CEREBROSPINAL FLUID GLUCOSE C SIP GLUCOSE CEREBROSPINAL FLUID CHEMISTRY GLUCOSE PROTEIN FETAL FIBRONECTIN FETAL FIBRONECTIN, VAGINAL FLUID STERILE CONTAINER STERILE CONTAINER STERILE CONTAINER 40-70 MG/DL 40-70 MG/DL 12-60 MG/DL NEGATIVE Page 10 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 URINE CHEMISTRY METHOD PANEL/TEST NAME NORMAL INTERVAL TEST(S) SPECIMEN TYPE/CONTAI NER AMYLASE URINE24 HRS 59-401 U/HR CALCIUM URINE24 HRS 42-353 MG/ 24 HRS RANDOM URINE* VARIABLE SAME AS FOR PLASMA AMYLASE SAME AS FOR PLASMA CALCIUM, TIMED SAME AS FOR PLASMA CALCIUM, RANDOM CALCIUM SAME AS FOR PLASMA CHLORIDE, TIMED CHLORIDE URINE24 HRS 110-250 MMOL/24 HRS SAME AS FOR PLASMA CHLORIDE, RANDOM CHLORIDE RANDOM URINE* VARIABLE SAME AS FOR PLASMA CREATININE, TIMED CREATININE URINE24 HRS 0.6-2.5 GM/ 24HRS SAME AS FOR PLASMA CREATININE, RANDOM CREATININE RANDOM URINE* VARIABLE SAME AS FOR PLASMA GLUCOSE, TIMED GLUCOSE SAME AS FOR PLASMA GLUCOSE, RANDOM SAME AS FOR PLASMA PHOSPHORUS, TIMED SAME AS FOR PLASMA PHOSPHORUS, RANDOM GLUCOSE URINE24 HRS < 0.5 GM/24HRS RANDOM URINE* 1-15 MG/DL PHOSPHORUS URINE24 HRS 0.4-1.3 GM/24HRS PHOSPHORUS RANDOM URINE* VARIABLE Page 11 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 SAME AS FOR PLASMA POTASSIUM, TIMED POTASSIUM URINE24 HRS POTASSIUM, RANDOM POTASSIUM RANDOM URINE* SAME AS FOR PLASMA URINE TOTAL PROTEIN, TIME TOTAL PROTEIN URINE24 HRS SAME AS FOR PLASMA URINE PROTEIN, RANDOM TOTAL PROTEIN RANDOM URINE* < 12.0 MG/DL SAME AS FOR PLASMA SODIUM, TIMED SODIUM URINE 24 HR 40-220 MMOL/24 HRS SAME AS FOR PLASMA SODIUM, RANDOM SODIUM RANDOM URINE* VARIABLE UREA NITROGEN, TIMED UREA NITROGEN URINE 24 HR 12-20 GM/24HRS UREA NITROGEN, RANDOM UREA NITROGEN RANDOM URINE* VARIABLE URIC ACID, TIMED URIC ACID URINE24 HR 50-990 MG/24HRS SAME AS FOR PLASMA URIC ACID, RANDOM URIC ACID N MICROALBUMIN, TIMED ALBUMIN N RATIO ALBUMIN/ CREATININE ALBUMIN URINE PREGNANCY TEST URINE HCG SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA SAME AS FOR PLASMA D RANDOM URINE* URINE 24 HR RANDOM URINE* FIRST MORNING URINE 25-125 MMOL/24HRS VARIABLE <150 MG/24 HR. VARIABLE <30.0 MG/24HRS <30.0 MG/G NEGATIVE Page 12 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 D R FP URINALYSIS PHYSICAL, CHEMICAL AND MICROSCOPIC ANALYSIS OF URINE DRUG SCREEN, URINE OSMOLALITY, URINE PHYSICAL, CHEMICAL AND MICROSCOPIC ANALYSIS OF URINE AMPHETAMINES BARBITURATES BENZODIAZEPINES CANNABINOIDS COCAINE METHADONE OPIATES PHENCYCLIDINE OSMOLALITY RANDOM URINE (3 ML) * RANDOM URINE * RANDOM URINE * YELLOW CLEAR GLUCOSE NEGATIVE PH-4.6-8.0 KETONES NEGATIVE PROTEIN BILRUBIN NEGATIVE BLOOD NEGATIVE UROBILINOGEN0.1 1-10 EHRLICH UNITS SPECIFIC GRAVITY 1.001-1.035 NITRITE NEGATIVE URINE LEUKOCYTE ESTERASE= NEGATIVE MICRO SCOPIC: RBC-P.3/HPF WBC-O.5/HPF HYALINE CASTS0-1/HPF CUTOFF CONCENTRATION (ng/mL): AMPHETAMINES 500 BARBITURATES 200 BENZODIAZEPINES 200 CANNABINOIDS 50 COCAINE 300 METHADONE 300 OPIATES 300 PHENCYCLIDINE 25 100-650 MOSMOL/KG *STERILE CONTAINER OR YELLOW TOP TUBE (NO ADDITIVE) Page 13 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 HEMATOLOGY METHOD PANEL/TEST NAME TEST(S) TUBE NORMAL INTERVAL FLUORESCE NT FLOW CYTOMETRY AUTOMATED BLOOD COUNT (CBC WITHOUT DIFFERENTIAL) WBC, RBC, HGB, HCT, MCH, MCHC, RDW,CPLT, MCV LAVENDER TOP See Appendix FLUORESCE NT FLOW CYTOMETRY CBC WITH AUTO DIFFERENTIAL DIFFERENTIAL WBC COUNT (STKS 5 PART) LAVENDER TOP See Appendix G-6 PD LAVENDER TOP See report CER GLUCOSE-6 PHOSPHATE DEHYDROGENASE KLEINHAUER BETKE STAIN FLUORESCE NT FLOW CYTOMETRY RESISTANCE OF FETAL HEMOGLOBIN TO ACID ELUTION LAVENDER TOP RETICULOCYTE AUTOMATED RETICULOCYTE COUNT LAVENDER TOP SEDIMENTATION RATE SEDIMENTATION RATE LAVENDER TOP SICKLE CELL PREPARATION TESTING FOR THE PRESENCE FOR THE PRESENCE OF SICKLING HEMOGLOBINS MANUAL SOLUBILITY TESTING FLOW TIME VISCOSITY, SERUM FLOW TIME VISCOSITY, WHOLE BLOOD VISCOSITY, SERUM VISCOSITY, WHOLE BLOOD LAVENDER TOP LESS THAN 1% OR NOT FETAL CELLS PRESENT NEW BORN 1.0-7.0% AFTER 6 MO. 0.4-2.5% AGE, 0-50 0-15 MM/HR MALE, AGE >50 0-20.0 MM/HR FEMALE, AGE>50 0-26.0 MM/HR NEGATIVE RED TOP NO GEL RATIO: 1.4-1.8 LAVENDER TOP RATIO: 4.8-5.2 Page 14 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 COAGULATION PANEL/TEST NAME TEST(S) TUBE NORMAL INTERVAL PHOTO-OPTICAL DETECTION D-DIMER D-DIMER BLUE TOP < 230 D-DU NG/ML PHOTO-OPTICAL DETECTION FACTOR II ASSAY FACTOR II ASSAY BLUE TOP 74-142% METHOD PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION CLAUSS METHOD ELECTROMECHANICAL PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION FACTOR V ASSAY FACTOR V ASSAY BLUE TOP 70-143% FACTOR VII ASSAY FACTOR VII ASSAY BLUE TOP 53-149% FACTOR VIII ASSAY FACTOR VIII ASSAY BLUE TOP 51-138% FACTOR IX ASSAY FACTOR IX ASSAY BLUE TOP 69-167% FACTOR X ASSAY FACTOR X ASSAY BLUE TOP 68-149% FACTOR XI ASSAY FACTOR XI ASSAY BLUE TOP 73-148% FACTOR XII ASSAY FACTOR XII ASSAY BLUE TOP 51-180% FIBRINOGEN FIBRINOGEN BLUE TOP 158 - 438 MG/DL LUPUS ANTICOAGULA NT PANEL Hexagonal DRVVT BLUE TOP SEE REPORT PLATELET AGGREGATION PLATELET AGGREGATION 8 BLUE TOPS AND 1 LAVENDER TOP HAND DELIVERED IMMEDIATELY AFTER COLLECTION SEE REPORT Page 15 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 HIGH-SHEAR FORCE DYNAMIC FLOW SYSTEM PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PHOTO-OPTICAL DETECTION PIFA TURBIDIMETRICBASED OPTICAL DETECTION TURBIDIMETRICBASED OPTICAL DETECTION 2 BLUE TOPS AND 1 LAVENDER TOP HAND DELIVERED IMMEDIATELY AFTER COLLECTION SEE REPORT PROTHROMBIN TIME BLUE TOP PT 10.0-13.1 SEC INR 0.87-1.16 PT MIXING STUDY BLUE TOP PLATELET FUNCTION ASSESMENT PLATELET FUNCTION ASSESMENT PROTHROMBIN TIME AND INR PT MIXING STUDY (APTT) ACTIVATED PARTIAL THROMBOPLASTIN TIME PARTIAL THROMBOPLASTIN TIME, ACTIVATED PTT MIXING STUDY PTT MIXING STUDY THROMBIN TIME THROMBIN TIME HEPARIN INDUCED THROMBOCYT OPENIA ANTIBODIES TO PF4 VERIFYNOW P2Y12 VERIFYNOW ASPIRIN HEPARIN INDUCED THROMBOCYT OPENIA ABS P2Y12 PLATELET RESPONSE (REACTIVITY) ASSAY ASPIRIN PLATELET RESPONSE (REACTIVITY) SEE REPORT BLUE TOP BLUE TOP BLUE TOP RED TOP TUBE NO GEL TWO 2 ML GREINER TUBES (PLEASE CONTACT LAB) HAND DELIVERED IMMEDIATELY AFTER COLLECTION TWO 2 ML GREINER TUBES (PLEASE CONTACT LAB) HAND DELIVERED IMMEDIATELY AFTER COLLECTION 27.5-37.4 SEC SEE REPORT 19.1-26.1 SEC NEGATIVE INTERPRETATION PROVIDED INTERPRETATION PROVIDED Page 16 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 SEROLOGY METHOD PANEL/TEST NAME BACTERIAL MENINGITIS PANEL IN SERUM LA BACTERIAL MENINGITIS PANEL IN URINE TEST(S) HEMOPHILUS INFLUENZA TYPE B, PNEUMOCCUS AND NEISSERIA A, B, C, AND Y MENINGITIDES SERO GROUPS TUBE CEREBROSPINAL FLUID NORMAL INTERVAL NEGATIVE NEGATIVE AS ABOVE LA URINE LA BACTERIAL MENINGITIS PANEL, SERUM EIA C. DIFFICILE GDH AND TOXINS A&B, STOOL CLOSTRIDIUM DIFFICILE GDH AND TOXINS A&B STERILE CONTAINER NEGATIVE CRYPTOCOCCAL ANTIGEN, SERUM CRYPTOCOCCAL ANTIGEN SCREEN AND TITER GOLD TOP NEGATIVE CRYPTOCOCCAL ANTIGEN SCREEN AND TITER CSF NEGATIVE LA LA CRYPTOCOCCAL ANTIGEN, CSF AS ABOVE GOLD TOP NEGATIVE Page 17 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 BIOFIRE FILMARRAY RVP RESPIRATORY PANEL, VIRAL AND BACTERIAL TARGETS STREPTO-COCCUS GROUP B ANTIGEN, CSF STREPTOCOCCUS GROUP B ANTIGEN STREPTO-COCCUS GROUP B ANTIGEN, SERUM STREPTOCOCCUS GROUP B ANTIGEN LA STREPTO-COCCUS GROUP B ANTIGEN URINE STREPTOCOCCUS GROUP B ANTIGEN CMIA HIV –1 &2 ANTIGEN/ANTIBODY HIV AG/AB SCREEN CMIA HEPATITIS A AB IG G HEPATITIS A AB IG G CMIA HEPATITIS A AB IG M CMIA HEPATITIS B CORE AB TOTAL HEPATITIS B CORE AB TOTAL CMIA HEPATITIS B CORE AB IGM HEPATITIS B CORE AB IGM CMIA HEPATITIS B SURFACE ANTIGEN PCR LA LA NASOPHARYNGEAL SWAB NEGATIVE CSF NEGATIVE GOLD TOP NEGATIVE 2ML - URINE NEGATIVE RED or GOLD TOP NEGATIVE RED or GOLD TOP NON-REACTIVE RED or GOLD TOP HEPATITIS A AB IG M NON-REACTIVE RED or GOLD TOP NON-REACTIVE RED or GOLD TOP NON-REACTIVE RED or GOLD TOP HEPATITIS B SURFACE ANTIGEN NON-REACTIVE RED or GOLD TOP CMIA HEPATITIS B SURFACE ANTIBODY HEPATITIS B SURFACE ANTIBODY EXPECTED VALUES: NON-IMMMUNE: NON REACTIVE IMMUNE: REACTIVE Page 18 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 CMIA HEPATITIS C AB HEPATITIS C AB DSI INFECTIOUS MONO-NUCLEOSIS HETEROPHILE ANTIBODIES MONOTEST LEGIONELLA LEGIONELLA URINARY ANTIGEN MRSA/MSSA PCR MRSA SCREEN RICA PCR STREPTOCOCCUS GROUP A ANTIGEN STREP A RICA RED or GOLD TOP GOLD TOP URINE STERILE CONTAINER OR GRAY TOP TUBE NASAL SWAB, COPAN TRANSPORT TUBE STERILE BBL CULTURESWAB PLUS AMIES GEL. DUAL SWAB FROM THE TONSILSAND/OR THE BACK OF THE THROAT NON-REACTIVE NEGATIVE NEGATIVE NEGATIVE NEGATIVE MISCELLANEOUS TEST NAME LEUKOCYTES IN STOOL, WRIGHT STAIN OCCULT BLOOD IN STOOL CLOTEST RAPID UREASE TEST FOR DIAGNOSIS OF H. PYLORI CONTAINER STERILE CONTAINER HEMOCCULT SLIDE OR STERILE CONTAINER GASTRIC BIOPSY IN STERILE SALINE, STERILE TUBE NORMAL INTERVAL NO WHITE BLOOD CELLS PRESENT NEGATIVE NEGATIVE Page 19 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 MALARIA SMEAR (AND OTHER BLOOD PARASITES) LAVENDER TOP CSF FLUID IN STERILE TUBE CEREBROSPINAL FLUID CELL COUNT BODY FLUIDS CELL COUNT NEGATIVE SEE RESULT REPORT PERITONEAL, PLEURAL, SYNOVIAL FLUID, ETC. COLLECTED IN LAVENDER TOP TUBE Tubes containing anticoagulants must be filled completely and mixed immediately after collection by gently inverting the tube several times. Gold or red top vacuum (serum) tubes contain clot activators and should also be mixed to accelerate clotting. Serum tubes with clot activators may be centrifuged about 20 minutes after collection. For coagulation tests draw a few milliliters of blood into a vacuum collection tube and discard. Then collect a completely filled blue top citrate tube. Mix by gently inverting several times, immediately after collection. To ensure accurate results all the specimens have to be delivered to the laboratory within maximum 2 hours from collection, unless stricter rules apply. The transport temperature for specimens in transit to the laboratory has to be room temperature, unless otherwise specified above. When specimens cannot be delivered to the laboratory within 2 hours from collection please contact the laboratory at 212 434 2300 for temperature storage recommendations. Blood collection tubes in use include: MINT 4.5 ml tube with lithium heparin and a gel barrier (plasma) GOLD 5 ml tube with gel barrier and clot activator (serum) RED 6 ml with clot activator (serum) BLUE 5 ml tube (4.5 ml draw) with citrate anticoagulant GRAY 6 ml tube with fluoride and oxalate Page 20 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 LAVENDER 3ml tube with EDTA anticoagulant DARK GREEN 4 ml tube with heparin anticoagulant GREINER 2 ml partial fill blue top tube containing 3.2% Sodium Citrate. The tubes must be filled to its intended whole blood capacity (indicated by small black line). Minimum volume for each test, unless otherwise specified above: 1 ml of whole blood, serum or plasma for chemistry tests 2 ml urine for urinalysis (macroscopic only) 3 ml urine for urinalysis (macroscopic and microscopic) 2 ml body fluids for chemistry and cell count 3 ml whole blood on EDTA for CBC (The tube must be more than half full for proper anticoagulant ratio. For pediatrics, draw blood in a MICROTAINER. Do not draw a small volume into a standard VACUTAINER). APPENDIX TEST AGE MALE NORMAL FEMALE NORMAL UNITS CBCD - Complete blood cell count w/automated differential WBC 0D 3D 7D 14D 1M 1 2 4 6 10 12 9.0-30.0 5.0-21.0 5.0-20.0 5.0-19.5 6.0-17.5 6.0-17.0 5.0-15.5 5.0-14.5 4.5-13.5 4.5-13.0 3.8-10.5 9.0-30.0 5.0-21.0 5.0-20.0 5.0-19.5 6.0-17.5 6.0-17.0 5.0-15.5 5.0-14.5 4.5-13.5 4.5-13.0 3.8-10.5 K/uL RBC 0D 1D 3D 7D 14D 1M 3.95-6.55 3.84-6.44 3.81-6.41 3.56-6.16 2.90-5.50 2.70-5.30 3.95-6.55 3.84-6.44 3.81-6.41 3.56-6.16 2.90-5.50 2.70-5.30 M/uL Page 21 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 HGB HCT MCV 2M 3M 6M 2 10 12 2.60-4.20 2.90-4.50 3.80-5.40 4.05-5.35 4.10-5.50 4.20-5.80 2.60-4.20 2.90-4.50 3.80-5.40 4.05-5.35 4.10-5.50 3.80-5.20 0D 1D 7D 21D 1M 2M 3M 6M 2 8 10 12.8-20.4 14.2-21.5 12.8-20.5 11.1-20.1 12.5-16.0 9.0-12.5 9.6-13.1 10.4-13.9 10.1-15.1 10.4-15.4 12.8-20.4 14.2-21.5 12.8-20.5 11.1-20.1 12.5-16.0 9.0-12.5 9.6-13.1 10.4-13.9 10.1-15.1 10.4-15.4 13.0-17.0 11.5-15.5 0D 1D 3D 7D 14D 21D 1M 2M 3M 6M 2 6 12 50.0-62.0 48.0-65.5 49.0-65.0 43.0-62.0 41.0-62.0 40.0-52.0 37.0-49.0 26.0-36.0 28.0-38.0 31.0-41.0 33.0-43.5 34.5-45.0 50.0-62.0 48.0-65.5 49.0-65.0 43.0-62.0 41.0-62.0 40.0-52.0 37.0-49.0 26.0-36.0 28.0-38.0 31.0-41.0 33.0-43.5 34.5-45.0 39.0-50.0 34.5-45.0 0D 1D 3D 7D 14D 21D 1M 2M 3M 6M 110.6-129.4 109.6-128.4 106.6-125.4 96.0-134.0 93.0-131.0 92.0-130.0 86.0-124.0 83.0-103.0 78.0-98.0 71.0-84.0 73.0-87.0 110.6-129.4 109.6-128.4 106.6-125.4 96.0-134.0 93.0-131.0 92.0-130.0 86.0-124.0 83.0-103.0 78.0-98.0 71.0-84.0 73.0-87.0 2 g/dL % fL Page 22 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 MCH 6 8 12 74.0-89.0 74.5-91.5 80.0-100.0 74.0-89.0 74.5-91.5 80.0-100.0 0D 1D 3D 7D 14D 21D 1M 2M 3M 6M 31.0-37.0 33.9-39.9 33.5-39.5 33.2-39.2 33.8-39.8 34.1-40.1 32.5-38.5 28.5-34.5 27.5-33.5 24.0-30.0 22.0-28.0 24.0-30.0 27.0-34.0 31.0-37.0 33.9-39.9 33.5-39.5 33.2-39.2 33.8-39.8 34.1-40.1 32.5-38.5 28.5-34.5 27.5-33.5 24.0-30.0 22.0-28.0 24.0-30.0 27.0-34.0 pg 29.7-33.7 29.6-33.6 29.1-33.1 30.0-34.0 30.1-34.1 31.9-35.9 31.5-35.5 32.1-36.1 32.8-36.8 32.0-36.0 31.0-35.0 32.0-36.0 31.0-35.0 32.0-36.0 29.7-33.7 29.6-33.6 29.1-33.1 30.0-34.0 30.1-34.1 31.9-35.9 31.5-35.5 32.1-36.1 32.8-36.8 32.0-36.0 31.0-35.0 32.0-36.0 31.0-35.0 32.0-36.0 g/dL 12.5-17.5 11.7-16.3 11.6-15.1 11.1-14.6 10.3-16.9 % 2 10 12 12.5-17.5 11.7-16.3 11.6-15.1 11.1-14.6 10.3-16.9 0D 1D 150-350 120-340 150-350 120-340 K/uL 1 4 12 MCHC 0D 1D 3D 7D 14D 21D 1M 2M 3M 6M 1 4 6 12 RDW 0D 2M PLT Page 23 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 7D 1M 120-370 150-400 120-370 150-400 AUTOMATED DIFFERENTIAL % NEUTROPHILS 0D 3D 7D 14D 1M 1 2 4 6 10 12 43.0-77.0 30.0-60.0 33.0-57.0 18.0-52.0 15.0-49.0 16.0-50.0 26.0-60.0 35.0-69.0 38.0-72.0 40.0-74.0 43.0-77.0 43.0-77.0 30.0-60.0 33.0-57.0 18.0-52.0 15.0-49.0 16.0-50.0 26.0-60.0 35.0-69.0 38.0-72.0 40.0-74.0 43.0-77.0 % % LYMPHOCYTES 0D 16.0-47.0 26.0-56.0 33.0-63.0 41.0-71.0 46.0-76.0 44.0-74.0 35.0-65.0 27.0-57.0 18.0-49.0 14.0-45.0 13.0-44.0 16.0-47.0 26.0-56.0 33.0-63.0 41.0-71.0 46.0-76.0 44.0-74.0 35.0-65.0 27.0-57.0 18.0-49.0 14.0-45.0 13.0-44.0 % 2.0-8.0 2.0-11.0 2.0-9.0 2.0-7.0 2.0-14.0 2.0-8.0 2.0-11.0 2.0-9.0 2.0-7.0 2.0-14.0 % 0.0-4.0 0.0-5.0 0.0-6.0 0.0-4.0 0.0-5.0 0.0-6.0 % 7D 10 0D 0.0-2.0 0.0-2.0 % 3D 7D 14D 1M 1 2 4 6 10 12 % MONOCYTES 0D 3D 14D 1M 12 % EOSINOPHILS % BASOPHILS 0D Related documents For Microbiology Specimens see policy 90.1.6.11.4 (TX. PC 885) Page 24 of 23 90.1.6.11.2 Specimen Collection: Clinical Laboratories, Blood Collection, Tube Requirements and Normal Intervals Effective Date: 12-01-15 100 East 77th Street New York, N.Y. 10075 Revision/Version No. Date Additions/Amendments 1 6/7/2010 New. Replacing 886TXPC with original issue date of 3/1998 2 02/16/2015 3 10/26/2015 4 12/01/2015 Removed: ACET, SLAP, RET Added: BHB Changed: ALPi Number reassigned from 886TXPC to 90.1.6.11.2 Changed reference ranges for: ALP, ALT, Ammonia, TBIL, IOPTH, Lactic acid, Phosphorus, Mg, BNP, Haptoglobin, Sodium (plasma), TT4, FT4, FT3, TSH, cortisol, Coagulation tests. Removed: Flu Antigen, RPR, UCRP, Homocysteine, RF, C3, C4, AFP, CEA, Fluid CEA, cortisol (random), Factor XIII deficiency screen, Hepatitis A Ig Total. Added: Osmolality serum and urine, Biofire RVP, VerifyNow testing, PIFA HIT, Indirect Bilirubin, Anion Gap, Appendix, eGFR, Strep A, MRSA/MSSA by PCR, GDH to the C. difficille testing, Ag to HIV screen, Hepatitis A Ig G. Updated reference range for PT from 9.4-13.0 to 10.0-13.1 sec. Added minimum volume requirements and discription of collection tubes. Page 25 of 23